Conc.: 0.5 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES / Details: This sample was monodisperse
Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 285 K Details: Blot time was for 4 seconds with 0 blot force before plunging.
-
Electron microscopy imaging
Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company
Microscopy
Model: FEI TALOS ARCTICA
Electron gun
Electron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: FLOOD BEAM
Average exposure time: 3 sec. / Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) / Num. of grids imaged: 1 / Num. of real images: 2683 / Details: Images were collected in movie-mode at
EM imaging optics
Energyfilter name: GIF Bioquantum / Energyfilter slit width: 20 eV
Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 260910 / Num. of class averages: 4 / Symmetry type: POINT
Atomic model building
Protocol: FLEXIBLE FIT / Space: REAL / Target criteria: Correlation coefficient Details: A initial model was from SWISS modeling using 6TGA as a template. Initial local fitting was done using Chimera and then ISOLDE plugged in ChimeraX for flexible fitting. The model was refined ...Details: A initial model was from SWISS modeling using 6TGA as a template. Initial local fitting was done using Chimera and then ISOLDE plugged in ChimeraX for flexible fitting. The model was refined by using Phenix and Coot.
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