a half map of MeFDH1_aN_b (only one set of half maps could be uploaded so rest half maps were uploaded as additional EM maps with a corresponding FSC curve)
a half map of MeFDH1_aN_b (only one set of half maps could be uploaded so rest half maps were uploaded as additional EM maps with a corresponding FSC curve)
Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 285 K / Instrument: FEI VITROBOT MARK IV Details: Blot time was for 4 seconds with 0 blot force before plunging..
Details
This sample was monodisperse
-
Electron microscopy
Microscope
FEI TALOS ARCTICA
Specialist optics
Energy filter - Name: GIF Bioquantum / Energy filter - Slit width: 20 eV
Image recording
Film or detector model: GATAN K3 (6k x 4k) / Digitization - Dimensions - Width: 5760 pixel / Digitization - Dimensions - Height: 4092 pixel / Number grids imaged: 1 / Number real images: 2683 / Average exposure time: 3.0 sec. / Average electron dose: 50.0 e/Å2 / Details: Images were collected in movie-mode at
Electron beam
Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
A initial model was from SWISS modeling using 6TGA as a template. Initial local fitting was done using Chimera and then ISOLDE plugged in ChimeraX for flexible fitting. The model was refined by using Phenix and Coot.
Refinement
Space: REAL / Protocol: FLEXIBLE FIT / Target criteria: Correlation coefficient
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