PDB-7dw5: Crystal structure of DUX4 HD1-HD2 domain complexed with ERG sites

ID or keywords:

Entry

Database: PDB / ID: 7dw5

Title

Crystal structure of DUX4 HD1-HD2 domain complexed with ERG sites

Components

(DNA (5'-D(P*CP*GP*AP*CP*TP*TP*GP*AP*TP*GP*AP*GP*AP*TP*TP*AP*GP*AP*CP*TP*G)-3')) x 2
Double homeobox protein 4-like protein 2

Keywords

DNA BINDING PROTEIN/DNA / acute lymphoblastic leukemia / DUX4/IGH / ERGalt / Clamp-like transactivation / DUX4-Responsive-Element / alternative splicing / DNA BINDING PROTEIN / DNA BINDING PROTEIN-DNA complex

Function / homology

Function and homology information

RNA polymerase II transcription regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity, RNA polymerase II-specific / chromatin / regulation of transcription by RNA polymerase II / nucleus
Similarity search - Function

Biological species

Homo sapiens (human)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 2.83 Å

Authors

Zhang, H. / Cheng, N. / Li, Z. / Zhang, W. / Dong, X. / Huang, J. / Meng, G.

Funding support

China, 2items

Citation

Journal: Cancer Commun (Lond) / Year: 2021
Title: DNA crosslinking and recombination-activating genes 1/2 (RAG1/2) are required for oncogenic splicing in acute lymphoblastic leukemia.
Authors: Zhang, H. / Cheng, N. / Li, Z. / Bai, L. / Fang, C. / Li, Y. / Zhang, W. / Dong, X. / Jiang, M. / Liang, Y. / Zhang, S. / Mi, J. / Zhu, J. / Zhang, Y. / Chen, S.J. / Zhao, Y. / Weng, X.Q. / ...

History

Deposition	Jan 15, 2021	Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0	Nov 3, 2021	Provider: repository / Type: Initial release
Revision 1.1	Feb 16, 2022	Group: Database references / Category: citation / citation_author Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID
Revision 1.2	Nov 29, 2023	Group: Data collection / Refinement description Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	7dw5.cif.gz	285.5 KB	Display	PDBx/mmCIF format
PDB format	pdb7dw5.ent.gz	230.9 KB	Display	PDB format
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Validation report

Summary document	7dw5_validation.pdf.gz	465.2 KB	Display	wwPDB validaton report
Full document	7dw5_full_validation.pdf.gz	470.6 KB	Display
Data in XML	7dw5_validation.xml.gz	13.6 KB	Display
Data in CIF	7dw5_validation.cif.gz	18.2 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/dw/7dw5 ftp://data.pdbj.org/pub/pdb/validation_reports/dw/7dw5	HTTPS FTP

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Related structure data

Related structure data	6a8rS S: Starting model for refinement
Similar structure data	3efl-d 2qu6-d 6v6a-d 1ogh-d 3dtw-d 6nag-d 6buu-d 5zv2-d 6itj-d 3b8q-d Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#67 - Jul 2005 TATA-Binding Protein similarity (1) #168 - Dec 2013 DNA Helicase similarity (1) #91 - Jul 2007 Thymine Dimers similarity (1) #255 - Mar 2021 Cisplatin and DNA similarity (1) #122 - Feb 2010 Enhanceosome similarity (2) #253 - Jan 2021 Expressome similarity (1) #181 - Jan 2015 Cascade and CRISPR similarity (1) #172 - Apr 2014 RecA and Rad51 similarity (1) #112 - Apr 2009 Oct and Sox Transcription Factors similarity (5) #150 - Jun 2012 Sliding Clamps similarity (1) #180 - Dec 2014 TAL Effectors similarity (1) #135 - Mar 2011 Integrase similarity (1) #84 - Dec 2006 Transposase similarity (1) #227 - Nov 2018 Telomerase similarity (1) #48 - Dec 2003 Catabolite Activator Protein similarity (1) #155 - Nov 2012 Vitamin D Receptor similarity (1) #40 - Apr 2003 RNA Polymerase similarity (1) #87 - Mar 2007 Zinc Fingers similarity (1) #73 - Jan 2006 Topoisomerase similarity (1) #3 - Mar 2000 DNA Polymerase similarity (1) #139 - Jul 2011 DNA Methyltransferase similarity (1) #131 - Nov 2010 Inteins similarity (1) #125 - May 2010 Parvoviruses similarity (1) #190 - Oct 2015 Two-component Systems similarity (1) #8 - Aug 2000 Restriction Enzymes similarity (1) #23 - Nov 2001 DNA similarity (1) #7 - Jul 2000 Nucleosome similarity (1) #119 - Nov 2009 Designed DNA Crystal similarity (1) #55 - Jul 2004 DNA Ligase similarity (1) #240 - Dec 2019 Hypoxia-Inducible Factors similarity (1) #45 - Sep 2003 Estrogen Receptor similarity (1) #39 - Mar 2003 lac Repressor similarity (1) #33 - Sep 2002 HIV Reverse Transcriptase similarity (1) #31 - Jul 2002 p53 Tumor Suppressor similarity (1) #160 - Apr 2013 Actinomycin similarity (1) #258 - Jun 2021 Glucocorticoid Receptor and Dexamethasone similarity (1) #257 - May 2021 Fetal Hemoglobin similarity (1) #92 - Aug 2007 Anabolic Steroids similarity (1) #241 - Jan 2020 Twenty Years of Molecules similarity (1) #220 - Apr 2018 Dehalogenases similarity (1)

PDB pages

PDBj /

wwPDB /

NCBI

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Deposited unit

A: Double homeobox protein 4-like protein 2

B: Double homeobox protein 4-like protein 2

E: DNA (5'-D(P*CP*GP*AP*CP*TP*TP*GP*AP*TP*GP*AP*GP*AP*TP*TP*AP*GP*AP*CP*TP*G)-3')

F: DNA (5'-D(P*CP*GP*AP*CP*TP*TP*GP*AP*TP*GP*AP*GP*AP*TP*TP*AP*GP*AP*CP*TP*G)-3')

C: DNA (5'-D(P*CP*GP*AP*CP*TP*TP*GP*AP*TP*GP*AP*GP*AP*TP*TP*AP*GP*AP*CP*TP*G)-3')

D: DNA (5'-D(P*CP*GP*AP*CP*TP*TP*GP*AP*TP*GP*AP*GP*AP*TP*TP*AP*GP*AP*CP*TP*G)-3')

hetero molecules

60.8 kDa, 6 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author&software
Evidence: SAXS

Unit cell

Length a, b, c (Å)	42.974, 64.451, 69.432
Angle α, β, γ (deg.)	95.82, 92.55, 103.31
Int Tables number	1
Space group name H-M	P1

#1: Protein	Double homeobox protein 4-like protein 2 Mass: 17483.920 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: DUX4L2 / Production host: Escherichia coli (E. coli) / References: UniProt: P0CJ85
#2: DNA chain	DNA (5'-D(PCPGPAPCPTPTPGPAPTPGPAPGPAPTPTPAPGPAPCPTPG)-3') Mass: 6502.220 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Homo sapiens* (human)
#3: DNA chain	DNA (5'-D(PCPGPAPCPTPTPGPAPTPGPAPGPAPTPTPAPGPAPCPTPG)-3') Mass: 6382.148 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Homo sapiens* (human)
#4: Chemical	ChemComp-BR / BROMIDE ION Mass: 79.904 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Br
#5: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 8 / Source method: isolated from a natural source / Formula: H₂O
Has ligand of interest	N

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 3.06 Å³/Da / Density % sol: 59.77 % Description: THE ENTRY CONTAINS FRIEDEL PAIRS IN I/F_PLUS/MINUS COLUMNS.
Crystal grow	Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: PEG3350

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Data collection

Diffraction	Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: SSRF / Beamline: BL19U1 / Wavelength: 0.9789 Å
Detector	Type: MAR CCD 165 mm / Detector: CCD / Date: Sep 20, 2018
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.9789 Å / Relative weight: 1
Reflection	Resolution: 2.83→41.7 Å / Num. obs: 12265 / % possible obs: 92 % / Redundancy: 2.9 % / CC_1/2: 0.99 / R_merge(I) obs: 0.18 / Net I/σ(I): 3.9
Reflection shell	Resolution: 2.83→2.85 Å / Redundancy: 2.6 % / R_merge(I) obs: 0.18 / Mean I/σ(I) obs: 0.4 / Num. unique obs: 453 / CC_1/2: 0.644 / % possible all: 85

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Processing

Software

Name	Version	Classification
PHENIX	(1.11.1_2575: ???)	refinement
HKL-3000		data reduction
SCALEPACK		data scaling
PHASER		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: 6A8R

6a8r

Resolution: 2.83→41.7 Å / SU ML: 0.32 / Cross valid method: FREE R-VALUE / σ(F): 1.83 / Phase error: 28.63

	R_factor	Num. reflection	% reflection
R_free	0.2416	559	4.56 %
R_work	0.2228	-	-
obs	0.2237	12254	71.21 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å

Refinement step

Cycle: LAST / Resolution: 2.83→41.7 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	2184	1722	1	8	3915

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.005	4176
X-RAY DIFFRACTION	f_angle_d	0.878	6001
X-RAY DIFFRACTION	f_dihedral_angle_d	27.305	2231
X-RAY DIFFRACTION	f_chiral_restr	0.045	633
X-RAY DIFFRACTION	f_plane_restr	0.005	501

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.83-3.1118	0.4974	50	0.3583	1453	X-RAY DIFFRACTION	35
3.1118-3.5618	0.2942	162	0.2615	2792	X-RAY DIFFRACTION	69
3.5618-4.4867	0.2462	168	0.2295	3550	X-RAY DIFFRACTION	86

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	0.9969	0.5927	-0.0717	1.7685	-0.0485	1.9528	0.0553	-0.2447	0.3099	0.2277	0.0817	0.3814	0.0521	-0.2657	0.0924	0.3407	-0.1279	0.1748	0.6229	0.0735	0.4092	2.9566	12.9343	50.6061
2	0.1811	0.1857	0.1347	0.3508	0.4022	0.5405	0.1583	-0.2513	-0.2105	-0.1849	-0.2966	0.4794	-0.1075	-0.729	-0.4197	0.1105	0.2023	-0.1637	0.5829	-0.0614	0.3567	-2.4746	21.097	36.1173
3	1.5648	1.2074	-0.5702	0.996	-0.3364	0.3567	-0.0345	-0.2453	0.5596	0.0024	0.2009	0.6617	-0.1564	-0.2286	-0.0941	0.158	0.3134	-0.0562	0.6381	-0.0188	0.4539	-2.2417	27.2701	45.0794
4	1.0501	-0.084	0.4438	0.4494	0.2017	1.4956	-0.2068	-0.1869	0.1948	0.0251	-0.1533	-0.0525	-0.0316	0.28	-0.8656	-0.3596	0.1889	-0.3352	0.3236	0.039	0.0819	6.3463	17.7715	37.9358
5	4.2447	1.6089	1.9575	2.9692	0.8444	0.9087	0.0325	-0.4702	-0.1105	0.5967	-0.1794	-0.1081	0.5333	-0.0051	-0.1476	0.4712	-0.0569	0.0115	0.5419	-0.03	0.0886	10.5003	10.4514	24.3317
6	5.543	-2.0202	-1.9237	8.2192	-2.8312	6.0294	0.2408	0.1934	-0.6448	-0.3067	0.2272	0.6821	0.0136	-0.5371	-0.1893	0.1471	0.2359	-0.0684	0.7086	-0.0519	0.3771	9.639	11.082	13.0365
7	8.8137	-7.132	-6.8808	6.0925	5.672	5.4265	0.0422	0.3465	0.2342	-0.4007	-0.1899	0.779	-0.4198	-0.5491	0.1255	1.56	-0.0304	0.6293	1.3504	-0.2197	1.4573	4.8497	2.6214	6.8111
8	0.763	-1.2726	0.3794	2.1456	-0.6202	0.1812	0.1161	0.0209	-0.0749	0.2393	-0.0689	0.0687	-0.3273	-0.2838	-0.2198	0.6132	-0.0699	-0.0475	0.5827	-0.1715	0.7955	19.5035	-10.1964	1.2857
9	1.4785	0.1282	-0.672	1.6257	0.3203	3.545	-0.1104	-0.2501	0.2453	0.5226	0.0006	-0.4781	-0.1588	0.2793	-0.3236	0.4809	0.0287	-0.3861	0.5081	-0.2343	0.3881	38.2361	-16.0204	3.6703
10	1.7948	-0.2015	0.0592	2.1665	1.4855	1.4953	0.163	-0.39	0.2173	-0.1476	0.1304	-0.944	-0.4065	0.55	-0.1246	0.3035	-0.0114	-0.0706	0.4289	-0.0845	0.4646	34.677	-7.7025	4.3896
11	6.8614	-2.9649	2.0642	2.0206	-0.5821	1.1436	-0.1147	-0.1897	-0.0119	0.3146	0.0489	-0.5312	0.0547	0.6038	-0.0492	0.747	-0.0439	0.1508	0.6364	0.0573	0.4633	33.2922	12.2903	-0.161
12	1.5412	1.2529	-1.6407	1.4946	-2.1987	3.331	-0.0367	0.4219	0.3571	-0.6689	-0.1089	0.1545	-0.7075	-0.0842	-0.0308	0.8883	0.1949	0.0333	0.4785	0.0503	0.5621	18.2432	16.2519	-1.6528
13	1.025	0.9841	-0.6552	1.0523	-0.7901	1.2488	-0.0102	0.6108	-0.0746	-1.1273	0.0813	0.0998	-0.0636	-0.0105	0.1534	0.5997	0.1357	-0.0219	0.7046	-0.1541	0.5409	16.8707	6.1835	-2.6597
14	1.9956	-0.0357	-0.7236	1.6935	-0.1654	1.6447	-0.1201	-0.1478	-0.3193	-0.2528	-0.2562	-0.4172	-0.057	0.5235	0.0393	0.4324	0.0415	-0.0056	0.4234	0.0774	0.2253	21.6195	9.8312	7.593
15	1.0581	0.3553	-0.639	0.6225	0.6059	2.7931	-0.0999	0.4161	0.1075	-0.4572	-0.0167	0.0122	-0.3466	0.2218	-0.115	0.6531	0.0965	-0.0595	0.529	0.0324	0.1489	11.3323	19.4118	22.6241
16	1.1177	0.6394	0.831	0.4919	0.5977	1.0235	0.1859	0.1569	-0.1857	-0.3544	0.0836	0.2881	0.3075	-0.137	-0.1554	1.0882	0.3087	-0.2852	0.6788	0.182	0.6803	4.2607	32.6579	27.4406
17	1.0315	-0.0517	0.3056	0.0585	-0.1133	0.2506	0.0302	-0.1699	-0.0165	-0.0431	-0.2039	-0.4224	0.0043	0.1866	0.4428	0.6285	0.088	-0.1417	0.3242	0.1269	0.3796	11.8008	35.568	46.5376
18	0.8441	0.0621	-0.3751	1.065	0.2747	1.2239	-0.2559	-0.3544	-0.1243	0.2072	0.1648	-0.0958	0.0491	-0.0165	0.0355	0.1615	0.0166	0.0137	0.2494	0.0136	0.1622	18.5808	25.6807	57.4405
19	0.7222	0.3967	1.0183	0.7751	-0.4016	3.3625	-0.4605	0.0843	0.2451	-0.6037	-0.2423	-0.1886	-1.3089	0.413	-0.8408	0.4712	-0.15	-0.0001	0.1878	-0.0089	0.5666	23.6294	36.4402	42.5535
20	1.1165	0.3424	-0.2341	2.3618	1.0453	3.2989	-0.0905	-0.4798	-0.3938	-0.2319	-0.6	0.3724	1.1923	-0.2377	-1.4985	0.5358	-0.0641	-0.0138	0.2752	0.1801	0.5666	9.7796	5.4051	42.6589
21	1.9679	0.1921	-0.3025	1.733	0.3793	2.0753	0.0354	-0.2002	-0.7991	-0.0092	-0.2681	-0.357	0.7304	0.1989	-0.1779	0.8251	-0.1801	0.2008	0.1986	0.098	0.8427	7.4827	-4.4357	40.2618
22	1.3221	0.4325	-0.02	0.7037	0.0085	0.8097	0.1215	-0.1689	-0.1278	-0.2145	-0.2678	-0.1447	-0.0612	0.1035	-0.0659	0.2086	-0.0777	0.07	0.2551	0.002	0.2016	16.7147	18.8071	42.5153
23	0.7864	-0.6884	-0.5643	1.6917	1.4597	1.2619	0.4027	-0.3562	0.6759	0.1038	-0.4934	0.107	-0.7115	0.044	0.1452	1.025	-0.3801	0.0531	0.5288	-0.0658	0.6532	21.6889	41.9027	44.5062
24	0.0225	-0.0019	0.0416	0.1613	-0.2701	0.5077	-0.0167	-0.0993	-0.0768	0.0292	-0.0601	-0.1263	-0.0456	0.1285	0.0295	1.0735	-0.5848	-0.2968	0.7567	0.4476	1.1111	32.628	48.7552	48.5101
25	2.5034	-0.0235	-1.335	3.4044	-0.9544	4.0199	-0.0952	-0.1683	-0.3277	-0.1014	0.048	0.8775	-0.0827	-0.6422	-0.0376	0.9971	-0.2559	0.0344	0.4826	0.0681	0.8176	17.0277	-23.9811	16.2873
26	0.8725	-0.1058	0.0553	1.0533	-0.3853	1.0589	0.2146	0.1774	0.1439	0.2347	-0.3219	-0.0471	0.2588	-0.2443	-0.0734	0.3343	0.0868	-0.0887	0.3184	-0.1362	0.3466	22.0632	-8.0455	8.9017
27	2.2146	1.2413	-0.9799	1.003	-0.6812	1.306	-0.1659	-0.5212	-0.0079	-0.708	-0.4146	-0.7993	-0.5673	0.7436	0.3125	0.622	-0.1924	0.1142	0.6025	-0.0107	0.7579	32.8605	13.651	12.3559
28	0.385	0.7939	0.5046	2.1724	1.3584	0.85	-0.0889	-0.164	0.3493	0.3705	0.0637	-0.6714	-0.3142	0.494	-0.0139	1.3027	-0.4703	-0.4036	1.0295	-0.3471	1.2274	34.8338	32.6953	10.2486
29	7.6756	1.2559	3.5773	2.3916	2.0837	3.8647	-0.4252	-0.1147	0.5047	0.224	-0.098	-0.7769	-0.8328	0.8174	0.2374	1.0315	-0.4122	0.0073	0.7451	0.0139	1.0354	36.2008	24.9566	15.0484
30	0.1357	0.1274	-0.1437	1.2237	0.0081	0.2423	0.6824	0.2011	0.6425	-0.5831	-0.6595	-0.2389	0.2507	0.55	-0.2391	0.5475	0.0729	0.146	0.4679	0.0348	0.3793	31.3275	8.9048	8.3692
31	0.851	-0.5833	-0.2625	2.7226	1.045	1.6967	-0.2794	-0.163	-0.6354	0.1013	-0.2244	0.1769	0.6918	-0.1019	0.2989	0.6818	-0.0349	0.1228	0.4088	-0.0356	0.6019	20.3833	-12.4566	12.9636
32	6.37	-5.7106	-3.1292	5.3204	3.0046	1.7352	0.0082	0.0931	-0.0241	-0.1242	-0.0254	-0.1786	0.2317	0.2553	-0.0609	0.7423	-0.2102	0.2269	0.6007	0.0863	1.1963	19.7495	-32.3081	12.0379

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 20 through 27 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 28 through 45 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 46 through 59 )
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 60 through 77 )
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 78 through 82 )
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 83 through 87 )
7	X-RAY DIFFRACTION	7	chain 'A' and (resid 88 through 92 )
8	X-RAY DIFFRACTION	8	chain 'A' and (resid 93 through 102 )
9	X-RAY DIFFRACTION	9	chain 'A' and (resid 103 through 115 )
10	X-RAY DIFFRACTION	10	chain 'A' and (resid 116 through 150 )
11	X-RAY DIFFRACTION	11	chain 'B' and (resid 20 through 27 )
12	X-RAY DIFFRACTION	12	chain 'B' and (resid 28 through 38 )
13	X-RAY DIFFRACTION	13	chain 'B' and (resid 39 through 59 )
14	X-RAY DIFFRACTION	14	chain 'B' and (resid 60 through 77 )
15	X-RAY DIFFRACTION	15	chain 'B' and (resid 78 through 87 )
16	X-RAY DIFFRACTION	16	chain 'B' and (resid 88 through 92 )
17	X-RAY DIFFRACTION	17	chain 'B' and (resid 93 through 102 )
18	X-RAY DIFFRACTION	18	chain 'B' and (resid 103 through 150 )
19	X-RAY DIFFRACTION	19	chain 'E' and (resid 1 through 10 )
20	X-RAY DIFFRACTION	20	chain 'E' and (resid 11 through 21 )
21	X-RAY DIFFRACTION	21	chain 'F' and (resid 1 through 5 )
22	X-RAY DIFFRACTION	22	chain 'F' and (resid 6 through 15 )
23	X-RAY DIFFRACTION	23	chain 'F' and (resid 16 through 20 )
24	X-RAY DIFFRACTION	24	chain 'F' and (resid 21 through 21 )
25	X-RAY DIFFRACTION	25	chain 'C' and (resid 1 through 5 )
26	X-RAY DIFFRACTION	26	chain 'C' and (resid 6 through 10 )
27	X-RAY DIFFRACTION	27	chain 'C' and (resid 11 through 20 )
28	X-RAY DIFFRACTION	28	chain 'C' and (resid 21 through 21 )
29	X-RAY DIFFRACTION	29	chain 'D' and (resid 1 through 5 )
30	X-RAY DIFFRACTION	30	chain 'D' and (resid 6 through 10 )
31	X-RAY DIFFRACTION	31	chain 'D' and (resid 11 through 20 )
32	X-RAY DIFFRACTION	32	chain 'D' and (resid 21 through 21 )

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