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基本情報
登録情報 | データベース: PDB / ID: 7dpz | ||||||
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タイトル | Cryo-EM structure of Coxsackievirus B1 virion in complex with CAR | ||||||
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![]() | VIRUS / Coxsackievirus B1 / CAR / Cryo-EM | ||||||
機能・相同性 | ![]() AV node cell-bundle of His cell adhesion involved in cell communication / cell adhesive protein binding involved in AV node cell-bundle of His cell communication / homotypic cell-cell adhesion / AV node cell to bundle of His cell communication / epithelial structure maintenance / regulation of AV node cell action potential / gamma-delta T cell activation / germ cell migration / apicolateral plasma membrane / transepithelial transport ...AV node cell-bundle of His cell adhesion involved in cell communication / cell adhesive protein binding involved in AV node cell-bundle of His cell communication / homotypic cell-cell adhesion / AV node cell to bundle of His cell communication / epithelial structure maintenance / regulation of AV node cell action potential / gamma-delta T cell activation / germ cell migration / apicolateral plasma membrane / transepithelial transport / cell-cell junction organization / connexin binding / cardiac muscle cell development / heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of RIG-I activity / intercalated disc / bicellular tight junction / cell adhesion molecule binding / picornain 2A / symbiont-mediated suppression of host mRNA export from nucleus / symbiont genome entry into host cell via pore formation in plasma membrane / mitochondrion organization / neutrophil chemotaxis / picornain 3C / acrosomal vesicle / T=pseudo3 icosahedral viral capsid / filopodium / PDZ domain binding / Cell surface interactions at the vascular wall / host cell cytoplasmic vesicle membrane / adherens junction / cytoplasmic vesicle membrane / neuromuscular junction / endocytosis involved in viral entry into host cell / beta-catenin binding / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / viral capsid / cell-cell junction / integrin binding / nucleoside-triphosphate phosphatase / cell junction / protein complex oligomerization / monoatomic ion channel activity / virus receptor activity / heart development / symbiont-mediated suppression of host gene expression / cell body / growth cone / actin cytoskeleton organization / basolateral plasma membrane / defense response to virus / DNA replication / host cell cytoplasm / RNA helicase activity / symbiont entry into host cell / neuron projection / induction by virus of host autophagy / membrane raft / RNA-directed RNA polymerase / viral RNA genome replication / cysteine-type endopeptidase activity / RNA-dependent RNA polymerase activity / signaling receptor binding / virus-mediated perturbation of host defense response / DNA-templated transcription / host cell nucleus / virion attachment to host cell / structural molecule activity / ATP hydrolysis activity / protein-containing complex / proteolysis / RNA binding / extracellular space / extracellular region / nucleoplasm / ATP binding / identical protein binding / metal ion binding / plasma membrane / cytoplasm 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.8 Å | ||||||
![]() | Li, S. / Zhu, R. / Xu, L. / Cheng, T. / Zheng, Q. | ||||||
![]() | ![]() タイトル: Cryo-EM structures reveal the molecular basis of receptor-initiated coxsackievirus uncoating. 著者: Longfa Xu / Qingbing Zheng / Rui Zhu / Zhichao Yin / Hai Yu / Yu Lin / Yuanyuan Wu / Maozhou He / Yang Huang / Yichao Jiang / Hui Sun / Zhenghui Zha / Hongwei Yang / Qiongzi Huang / Dongqing ...著者: Longfa Xu / Qingbing Zheng / Rui Zhu / Zhichao Yin / Hai Yu / Yu Lin / Yuanyuan Wu / Maozhou He / Yang Huang / Yichao Jiang / Hui Sun / Zhenghui Zha / Hongwei Yang / Qiongzi Huang / Dongqing Zhang / Zhenqin Chen / Xiangzhong Ye / Jinle Han / Lisheng Yang / Che Liu / Yuqiong Que / Mujin Fang / Ying Gu / Jun Zhang / Wenxin Luo / Z Hong Zhou / Shaowei Li / Tong Cheng / Ningshao Xia / ![]() ![]() 要旨: Enterovirus uncoating receptors bind at the surface depression ("canyon") that encircles each capsid vertex causing the release of a host-derived lipid called "pocket factor" that is buried in a ...Enterovirus uncoating receptors bind at the surface depression ("canyon") that encircles each capsid vertex causing the release of a host-derived lipid called "pocket factor" that is buried in a hydrophobic pocket formed by the major viral capsid protein, VP1. Coxsackievirus and adenovirus receptor (CAR) is a universal uncoating receptor of group B coxsackieviruses (CVB). Here, we present five high-resolution cryoEM structures of CVB representing different stages of virus infection. Structural comparisons show that the CAR penetrates deeper into the canyon than other uncoating receptors, leading to a cascade of events: collapse of the VP1 hydrophobic pocket, high-efficiency release of the pocket factor and viral uncoating and genome release under neutral pH, as compared with low pH. Furthermore, we identified a potent therapeutic antibody that can neutralize viral infection by interfering with virion-CAR interactions, destabilizing the capsid and inducing virion disruption. Together, these results define the structural basis of CVB cell entry and antibody neutralization. | ||||||
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構造の表示
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構造ビューア | 分子: ![]() ![]() |
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PDBx/mmCIF形式 | ![]() | 169.8 KB | 表示 | ![]() |
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PDB形式 | ![]() | 138.6 KB | 表示 | ![]() |
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-検証レポート
文書・要旨 | ![]() | 955 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 962.7 KB | 表示 | |
XML形式データ | ![]() | 31.9 KB | 表示 | |
CIF形式データ | ![]() | 46.9 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
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対称性 | 点対称性: (シェーンフリース記号: I (正20面体型対称)) |
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要素
#1: タンパク質 | 分子量: 31207.117 Da / 分子数: 1 / 由来タイプ: 天然 由来: (天然) ![]() 参照: UniProt: W8GTF7 |
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#2: タンパク質 | 分子量: 29122.744 Da / 分子数: 1 / 由来タイプ: 天然 由来: (天然) ![]() 参照: UniProt: A0A2S0RQC2 |
#3: タンパク質 | 分子量: 26328.764 Da / 分子数: 1 / 由来タイプ: 天然 由来: (天然) ![]() 参照: UniProt: L7UV52 |
#4: タンパク質 | 分子量: 7484.246 Da / 分子数: 1 / 由来タイプ: 天然 由来: (天然) ![]() 参照: UniProt: A0A2S1FMR1 |
#5: タンパク質 | 分子量: 13471.361 Da / 分子数: 1 / Fragment: D1 domain / 由来タイプ: 天然 / 由来: (天然) ![]() |
-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
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試料調製
構成要素 | 名称: Coxsackievirus B1 / タイプ: VIRUS / Entity ID: all / 由来: NATURAL |
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由来(天然) | 生物種: ![]() |
ウイルスについての詳細 | 中空か: YES / エンベロープを持つか: NO / 単離: STRAIN / タイプ: VIRION |
緩衝液 | pH: 7.4 |
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
急速凍結 | 凍結剤: ETHANE |
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電子顕微鏡撮影
実験機器 | ![]() モデル: Tecnai F30 / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TECNAI F30 |
電子銃 | 電子線源: ![]() |
電子レンズ | モード: BRIGHT FIELD |
撮影 | 電子線照射量: 25 e/Å2 フィルム・検出器のモデル: FEI FALCON III (4k x 4k) |
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解析
ソフトウェア | 名称: PHENIX / バージョン: 1.15.2_3472: / 分類: 精密化 | ||||||||||||||||||||||||
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CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
3次元再構成 | 解像度: 3.8 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 6538 / 対称性のタイプ: POINT | ||||||||||||||||||||||||
拘束条件 |
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