+Open data
-Basic information
Entry | Database: PDB / ID: 7a0n | ||||||
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Title | Structure of TSC1 NTD and linker domain | ||||||
Components | Uncharacterized protein,Uncharacterized protein | ||||||
Keywords | SIGNALING PROTEIN / TSC1 / GAP / GTPase | ||||||
Function / homology | Hamartin / Hamartin protein / Armadillo-type fold / Hamartin Function and homology information | ||||||
Biological species | Chaetomium thermophilum (fungus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 4.3 Å | ||||||
Authors | Fitzian, K. / Kuemmel, D. | ||||||
Funding support | Germany, 1items
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Citation | Journal: Mol.Cell / Year: 2021 Title: TSC1 binding to lysosomal PIPs is required for TSC complex translocation and mTORC1 regulation. Authors: Fitzian, K. / Bruckner, A. / Brohee, L. / Zech, R. / Antoni, C. / Kiontke, S. / Gasper, R. / Linard Matos, A.L. / Beel, S. / Wilhelm, S. / Gerke, V. / Ungermann, C. / Nellist, M. / Raunser, ...Authors: Fitzian, K. / Bruckner, A. / Brohee, L. / Zech, R. / Antoni, C. / Kiontke, S. / Gasper, R. / Linard Matos, A.L. / Beel, S. / Wilhelm, S. / Gerke, V. / Ungermann, C. / Nellist, M. / Raunser, S. / Demetriades, C. / Oeckinghaus, A. / Kummel, D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7a0n.cif.gz | 323.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7a0n.ent.gz | 264.9 KB | Display | PDB format |
PDBx/mmJSON format | 7a0n.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a0/7a0n ftp://data.pdbj.org/pub/pdb/validation_reports/a0/7a0n | HTTPS FTP |
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-Related structure data
Related structure data | 7a0mSC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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3 |
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Unit cell |
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-Components
#1: Protein | Mass: 54452.883 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus) Strain: DSM 1495 / CBS 144.50 / IMI 039719 / Gene: CTHT_0033260 / Production host: Escherichia coli (E. coli) / References: UniProt: G0S5K3 Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 5.47 Å3/Da / Density % sol: 77.53 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, sitting drop Details: 0.1 M HEPES pH 7.3, 30 % glycerol, 7 % 2-propanol, 0.01 M phenol |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: PETRA III, EMBL c/o DESY / Beamline: P13 (MX1) / Wavelength: 0.9802 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Jun 28, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9802 Å / Relative weight: 1 |
Reflection | Resolution: 4.3→20 Å / Num. obs: 27707 / % possible obs: 98.7 % / Redundancy: 15.5 % / CC1/2: 0.994 / Rrim(I) all: 0.367 / Net I/σ(I): 6.7 |
Reflection shell | Resolution: 4.3→4.53 Å / Mean I/σ(I) obs: 1.9 / Num. unique obs: 3968 / CC1/2: 0.917 / Rrim(I) all: 2.285 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 7A0M Resolution: 4.3→19.993 Å / SU ML: 0.61 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 37.5 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 4.3→19.993 Å
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Refine LS restraints |
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LS refinement shell |
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