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- PDB-6yei: Arabidopsis thaliana glutamate dehydrogenase isoform 1 in complex... -

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Basic information

Entry
Database: PDB / ID: 6yei
TitleArabidopsis thaliana glutamate dehydrogenase isoform 1 in complex with NAD
ComponentsGlutamate dehydrogenase 1
KeywordsOXIDOREDUCTASE / glutamate dehydrogenase / 2-oxoglutarate / NAD / amino acid metabolism
Function / homology
Function and homology information


response to absence of light / glutamate dehydrogenase [NAD(P)+] activity / glutamate dehydrogenase [NAD(P)+] / glutamate dehydrogenase (NAD+) activity / glutamate dehydrogenase (NADP+) activity / amino acid metabolic process / cobalt ion binding / plastid / copper ion binding / mitochondrion ...response to absence of light / glutamate dehydrogenase [NAD(P)+] activity / glutamate dehydrogenase [NAD(P)+] / glutamate dehydrogenase (NAD+) activity / glutamate dehydrogenase (NADP+) activity / amino acid metabolic process / cobalt ion binding / plastid / copper ion binding / mitochondrion / zinc ion binding / ATP binding
Similarity search - Function
Glutamate dehydrogenase / Leucine Dehydrogenase, chain A, domain 1 / NAD(P) binding domain of glutamate dehydrogenase / Leu/Phe/Val dehydrogenases active site / Glu / Leu / Phe / Val dehydrogenases active site. / Glutamate/phenylalanine/leucine/valine dehydrogenase / Glutamate/phenylalanine/leucine/valine dehydrogenase, dimerisation domain / Glu/Leu/Phe/Val dehydrogenase, dimerisation domain / Glutamate/Leucine/Phenylalanine/Valine dehydrogenase / Glutamate/phenylalanine/leucine/valine dehydrogenase, C-terminal ...Glutamate dehydrogenase / Leucine Dehydrogenase, chain A, domain 1 / NAD(P) binding domain of glutamate dehydrogenase / Leu/Phe/Val dehydrogenases active site / Glu / Leu / Phe / Val dehydrogenases active site. / Glutamate/phenylalanine/leucine/valine dehydrogenase / Glutamate/phenylalanine/leucine/valine dehydrogenase, dimerisation domain / Glu/Leu/Phe/Val dehydrogenase, dimerisation domain / Glutamate/Leucine/Phenylalanine/Valine dehydrogenase / Glutamate/phenylalanine/leucine/valine dehydrogenase, C-terminal / Glutamate/Leucine/Phenylalanine/Valine dehydrogenase / Aminoacid dehydrogenase-like, N-terminal domain superfamily / NAD(P)-binding Rossmann-like Domain / NAD(P)-binding domain superfamily / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
2-OXOGLUTARIC ACID / : / NICOTINAMIDE-ADENINE-DINUCLEOTIDE / Glutamate dehydrogenase 1
Similarity search - Component
Biological speciesArabidopsis thaliana (thale cress)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.02 Å
AuthorsRuszkowski, M. / Grzechowiak, M. / Jaskolski, M.
Funding support Poland, 1items
OrganizationGrant numberCountry
Polish National Science CentreSONATA 2018/31/D/NZ1/03630 Poland
CitationJournal: Front Plant Sci / Year: 2020
Title: Structural Studies of Glutamate Dehydrogenase (Isoform 1) FromArabidopsis thaliana, an Important Enzyme at the Branch-Point Between Carbon and Nitrogen Metabolism.
Authors: Grzechowiak, M. / Sliwiak, J. / Jaskolski, M. / Ruszkowski, M.
History
DepositionMar 24, 2020Deposition site: PDBE / Processing site: PDBE
Revision 1.0May 20, 2020Provider: repository / Type: Initial release
Revision 1.1Jul 22, 2020Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.identifier_ORCID
Revision 1.2Jan 24, 2024Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Glutamate dehydrogenase 1
B: Glutamate dehydrogenase 1
C: Glutamate dehydrogenase 1
D: Glutamate dehydrogenase 1
E: Glutamate dehydrogenase 1
F: Glutamate dehydrogenase 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)275,97441
Polymers269,1256
Non-polymers6,84935
Water13,637757
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area32810 Å2
ΔGint-257 kcal/mol
Surface area84490 Å2
MethodPISA
Unit cell
Length a, b, c (Å)93.843, 99.811, 317.948
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Space group name HallP2ac2ab
Symmetry operation#1: x,y,z
#2: x+1/2,-y+1/2,-z
#3: -x,y+1/2,-z+1/2
#4: -x+1/2,-y,z+1/2
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-ID
11
21
31
41
51
61

NCS domain segments:

Ens-ID: 1

Dom-IDComponent-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11ALAALAPHEPHE(chain 'A' and (resid 6 through 32 or resid 34...AA6 - 329 - 35
12GLUGLUTHRTHR(chain 'A' and (resid 6 through 32 or resid 34...AA34 - 12537 - 128
13LYSLYSLEULEU(chain 'A' and (resid 6 through 32 or resid 34...AA127 - 179130 - 182
14ASPASPASNASN(chain 'A' and (resid 6 through 32 or resid 34...AA182 - 312185 - 315
15PROPROTRPTRP(chain 'A' and (resid 6 through 32 or resid 34...AA314 - 409317 - 412
26ALAALAPHEPHE(chain 'B' and (resid 6 through 32 or resid 34...BB6 - 329 - 35
27GLUGLUTHRTHR(chain 'B' and (resid 6 through 32 or resid 34...BB34 - 12537 - 128
28LYSLYSLEULEU(chain 'B' and (resid 6 through 32 or resid 34...BB127 - 179130 - 182
29ASPASPASNASN(chain 'B' and (resid 6 through 32 or resid 34...BB182 - 312185 - 315
210PROPROTRPTRP(chain 'B' and (resid 6 through 32 or resid 34...BB314 - 409317 - 412
311ALAALAPHEPHE(chain 'C' and (resid 6 through 32 or resid 34...CC6 - 329 - 35
312GLUGLUTHRTHR(chain 'C' and (resid 6 through 32 or resid 34...CC34 - 12537 - 128
313LYSLYSLEULEU(chain 'C' and (resid 6 through 32 or resid 34...CC127 - 179130 - 182
314ASPASPASNASN(chain 'C' and (resid 6 through 32 or resid 34...CC182 - 312185 - 315
315PROPROTRPTRP(chain 'C' and (resid 6 through 32 or resid 34...CC314 - 409317 - 412
416ALAALAPHEPHE(chain 'D' and (resid 6 through 32 or resid 34...DD6 - 329 - 35
417GLUGLUTHRTHR(chain 'D' and (resid 6 through 32 or resid 34...DD34 - 12537 - 128
418LYSLYSLEULEU(chain 'D' and (resid 6 through 32 or resid 34...DD127 - 179130 - 182
419ASPASPASNASN(chain 'D' and (resid 6 through 32 or resid 34...DD182 - 312185 - 315
420PROPROTRPTRP(chain 'D' and (resid 6 through 32 or resid 34...DD314 - 409317 - 412
521ALAALAPHEPHE(chain 'E' and (resid 6 through 32 or resid 34...EE6 - 329 - 35
522GLUGLUTHRTHR(chain 'E' and (resid 6 through 32 or resid 34...EE34 - 12537 - 128
523LYSLYSLEULEU(chain 'E' and (resid 6 through 32 or resid 34...EE127 - 179130 - 182
524ASPASPASNASN(chain 'E' and (resid 6 through 32 or resid 34...EE182 - 312185 - 315
525PROPROTRPTRP(chain 'E' and (resid 6 through 32 or resid 34...EE314 - 409317 - 412
626ALAALAPHEPHE(chain 'F' and (resid 6 through 32 or resid 34...FF6 - 329 - 35
627GLUGLUTHRTHR(chain 'F' and (resid 6 through 32 or resid 34...FF34 - 12537 - 128
628LYSLYSLEULEU(chain 'F' and (resid 6 through 32 or resid 34...FF127 - 179130 - 182
629ASPASPASNASN(chain 'F' and (resid 6 through 32 or resid 34...FF182 - 312185 - 315
630PROPROTRPTRP(chain 'F' and (resid 6 through 32 or resid 34...FF314 - 409317 - 412

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Components

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Protein , 1 types, 6 molecules ABCDEF

#1: Protein
Glutamate dehydrogenase 1 / GDH 1


Mass: 44854.219 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Gene: GDH1, At5g18170, MRG7.13 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21-Gold (DE3)
References: UniProt: Q43314, glutamate dehydrogenase [NAD(P)+]

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Non-polymers , 7 types, 792 molecules

#2: Chemical
ChemComp-NAD / NICOTINAMIDE-ADENINE-DINUCLEOTIDE


Mass: 663.425 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: C21H27N7O14P2 / Feature type: SUBJECT OF INVESTIGATION / Comment: NAD*YM
#3: Chemical
ChemComp-MPD / (4S)-2-METHYL-2,4-PENTANEDIOL


Mass: 118.174 Da / Num. of mol.: 15 / Source method: obtained synthetically / Formula: C6H14O2 / Comment: precipitant*YM
#4: Chemical
ChemComp-K / POTASSIUM ION


Mass: 39.098 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: K / Feature type: SUBJECT OF INVESTIGATION
#5: Chemical
ChemComp-MRD / (4R)-2-METHYLPENTANE-2,4-DIOL


Mass: 118.174 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C6H14O2 / Comment: precipitant*YM
#6: Chemical ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C2H6O2
#7: Chemical ChemComp-AKG / 2-OXOGLUTARIC ACID


Mass: 146.098 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Formula: C5H6O5 / Feature type: SUBJECT OF INVESTIGATION
#8: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 757 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.79 Å3/Da / Density % sol: 55.89 %
Crystal growTemperature: 292 K / Method: vapor diffusion, sitting drop / pH: 6.5 / Details: 15% w/v PEG 6000, 100 mM MES pH 6.5, 10% (v/v) MPD

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: PETRA III, EMBL c/o DESY / Beamline: P13 (MX1) / Wavelength: 0.9763 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jul 17, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9763 Å / Relative weight: 1
ReflectionResolution: 2.02→70.3 Å / Num. obs: 164077 / % possible obs: 95.7 % / Redundancy: 7.4 % / Biso Wilson estimate: 35.19 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.071 / Rpim(I) all: 0.028 / Net I/σ(I): 17.3
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. unique obsCC1/2Rpim(I) all% possible all
2.02-2.147.30.8042.581940.7810.44964.8
5.94-70.36.90.02748.882040.9990.01599.8

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Processing

Software
NameVersionClassification
PHENIX1.16_3549refinement
XDSdata reduction
XSCALEdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1b26

1b26


Resolution: 2.02→70.26 Å / SU ML: 0.1996 / Cross valid method: FREE R-VALUE / Phase error: 22.4684
Details: Hydrogen atoms at riding positions were added during refinement
RfactorNum. reflection% reflectionSelection details
Rfree0.196 1646 1 %random
Rwork0.1604 ---
obs0.1607 164061 83.28 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso mean: 53.86 Å2
Refinement stepCycle: LAST / Resolution: 2.02→70.26 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms18639 0 448 757 19844
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.007919509
X-RAY DIFFRACTIONf_angle_d0.952726442
X-RAY DIFFRACTIONf_chiral_restr0.05842953
X-RAY DIFFRACTIONf_plane_restr0.00673390
X-RAY DIFFRACTIONf_dihedral_angle_d15.219411691
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.46394366292-0.2459794094120.7306174803870.761182149536-0.4073450892572.54760786573-0.0214605204829-0.04672959186250.2514135868950.0986582947659-0.03096770076550.0791926676828-0.256635579003-0.162209375810.08387032949020.2054602624070.031993711994-0.0009256201946180.212740312382-0.07845476725650.28022417867613.887365071316.9433237204135.565780885
21.89410477502-0.873120400845-1.087397736241.493715496620.915699836361.46069168482-0.0475425353982-0.2305476212980.007582369994620.272127276372-0.05189085582210.1559897557440.0505937315851-0.1003769521790.1087274702240.281537843053-0.03119852368310.02674659769690.242311996897-0.05453734886140.2205055252263.3347779058811.2655541568155.296617936
32.884755842340.0768924943572-1.157488522516.42261911079-3.17803959084.551206686340.1031776309330.1124680162070.8204868080580.3689730415340.2129756235710.247197978619-1.06935494546-0.448962906772-0.272377947980.4791071726750.1340555137930.02792814349830.2837687499780.008205780917990.45425284152216.962523077837.3300372795115.297567585
42.57144404247-0.4260775846161.516818225471.67314360826-0.7983801667734.59778250849-0.081119116673-0.2375807540280.1981543770070.123450926830.0751500429501-0.0581402581752-0.4662659327560.164260565410.04252008598070.2252965850690.01649108369850.01047798512140.240975086691-0.08838536933040.28301559640132.494208088721.817484745128.272300526
52.47413483023-1.024049076130.8285207193571.73440197717-0.7208267127612.97804005486-0.0131121257962-0.1071222584590.2124695914330.04408593302940.0397557524947-0.158975689876-0.139568661160.144193946981-0.02093090196760.172908066588-0.009202576180020.01243191735090.172386550069-0.06956775626430.25647560402235.581671314820.024635749122.314782807
61.46091283121.04133152079-0.8541137616637.17289373478-2.989547127262.50543166918-0.02240105184990.09276843894030.0372720282234-0.2404862335150.0733013698279-0.488117857911-0.1521159043120.1117877018440.01485590613120.298332558024-0.00849335980440.03488370655580.280889293785-0.09509781289790.28796206090242.39162018626.6805965796105.724331255
73.025351422650.7641917800280.3657150039793.87642146234-0.06718203434192.89955603572-0.02375594217130.1436351755520.591076950822-0.07845644842570.327489638348-1.05763436555-0.6614429321510.860042033896-0.2861421082530.498066562765-0.140868488340.1225668758270.492990602097-0.1987435267890.79191108538252.69484121143.5764198806105.05205791
84.81498213667-0.3143562292220.6442176539182.341988663520.6696507495292.57810965353-0.100816036226-0.2666440464620.618978300640.2655067958870.289257973192-1.08205243455-0.8112460280591.0930075347-0.1692518079850.614094065443-0.205688045750.01598704804140.736058298749-0.2919588225961.0210407931256.948322768745.5484597822111.756949017
92.28289796384-1.075979125280.4335576021863.000377886331.670014746051.48755573115-0.139965715831-0.4707581304691.24763915070.356685067510.0513606798276-0.204975696723-1.267256654510.2804455054920.03743010513310.897878565016-0.09667125057240.1067746292830.353118933439-0.1594573974190.76081341393540.592171666552.2292762406112.416479442
101.65188889984-0.1935641986610.4077701786517.085524022940.7452704362481.446735279760.0306964247942-0.1427388511080.425124789337-0.005586222298360.01289879203750.065753270927-0.4902214149150.0198579396375-0.05922764392340.3489690159370.009931385349230.09254090637610.235623237095-0.04809154794070.32402406256436.551418433134.4358803672107.637575292
114.3402612222-4.39801343482-1.086573374514.727341735941.0572935712.952382832230.5502002996580.5731865400080.29677064481-1.20677367467-0.2641332862320.110413889859-0.351610670711-0.175175700893-0.2234463534920.446506091179-0.04095265216330.02336241904240.2935785576070.01676579492750.41690745678133.495881410132.804913818597.6689076369
123.443588723562.18691349106-0.7166011895536.151034670840.3982545101392.358448340420.07282874410240.09552331535620.736726804674-0.317594318037-0.00698210003690.556639016941-0.477065282867-0.163625667182-0.1118240338490.3402899409740.126921484158-0.02539072320670.2446009940380.01799411291490.32250848139119.411371135733.7103224711107.098209978
131.570378647970.945895497357-0.1423829006251.794402197750.4157705887842.23742592728-0.0604133636225-0.0375480728533-0.2968343825270.1458950765950.0947092662587-0.2480579208760.5314999792070.295443401146-0.06363340748840.313648798440.117404703811-0.04166361495860.221428728688-0.04033804273250.29937220884337.3980770065-15.5013121866131.503486659
141.691363196750.2826142993260.04335659134783.245617925640.3987976690342.41604978052-0.0124797332833-0.46194661-0.6316897731531.027104611660.0777769404956-0.9038485573620.9524850253190.289599996389-0.01151354659110.8292041828780.140424165487-0.1743768961720.3653026612870.08430116006780.64388171866530.4396480954-28.5260719598149.435370443
155.243480512790.283248521998-2.404116800952.17031243767-1.364162673811.797646681080.240149251175-0.222709610639-0.7461373773610.374228562602-0.161449874079-0.9333601753080.4154771801061.045921234680.09597106479120.2183966759590.143523921365-0.06437868716530.639878722306-0.114889170880.57843880104160.1562685391-3.49696958829118.464112514
160.8395605241490.250656672465-0.3859079829931.678156183690.2870379749651.387452432940.009330384199810.0749291366355-0.16111675951-0.08959651597040.0117943684358-0.1185651777060.2284841228270.117312145684-0.0006977013576070.2405914034810.0794642677351-0.02622742835890.241129722346-0.08547990870660.30028980877341.2146138399-11.4288402703104.482697911
172.66370326496-0.115744446962-0.4397544838663.008526496481.328125147823.86492348453-0.01678410196970.124859394525-0.262230597724-0.2321344193440.628794676362-1.040324237620.8998917345081.17106888976-0.2916071522620.5763524403620.1670090008440.1389100465220.645111068134-0.2961688340080.70684752070564.4020742221-10.838778509486.6716649739
182.95616051557-0.2251160400320.1265925836731.144369656160.3838662304043.402478478710.01021275551210.1618687814250.0840796826122-0.1582962886990.154707016856-0.359736804345-0.2542705768690.553823588713-0.1315873887870.2113283115780.01597523489620.03921793424570.235205123411-0.08127530443340.32377576679651.99264034285.0963296298102.449691555
191.08525790709-0.873530127622-0.8414609730141.930301240940.4733875513241.808435207680.04198229628240.163683837395-0.150824463679-0.225213695978-0.0386582212430.2647860292750.216760315088-0.374127254333-0.01541166118390.268138728637-0.0965226628684-0.04349359589410.404833674118-0.1156100928550.3036548527752.59202932608-13.6530184097111.771649046
202.21233092359-1.630744964380.9678669603793.39190791464-1.799088122173.538898347920.278717476228-0.189222381876-1.14023177445-0.4545573859590.4476993496951.54296349881.28866358594-1.24040713297-0.07800482503960.667719881802-0.511941059575-0.2151881159750.728408894290.03232860237831.02701132679-12.1395912321-24.196416872127.441050635
211.129438079060.292805100318-0.2599357821091.346074888920.4428535765611.49014987331-0.01604535095740.2153302912110.0498003071574-0.242745480729-0.03990897243850.227031626009-0.0379482437096-0.3682265686110.05746135743930.2218136216280.047528586768-0.07398441351660.382804418761-0.01157236504570.2357549156589.195533838624.3963363220697.5809491672
222.92225852016-0.9725890499260.1904190906271.867059006840.2672699673392.46232763771-0.09630291013620.546110359050.31382734257-0.5072784522590.0457064175045-0.0317340976157-0.354916652122-0.2630723757920.04723194794490.603081020525-0.0525396249828-0.1302224377520.5943972680040.1268287696630.3676694386629.5755469096118.647210415174.2283036509
235.87684965245-1.15671639375-0.2360999106667.548267368950.9951352666180.152837893675-0.2152532127830.2673884051640.463712313297-0.2552411218020.1493990352570.548226722309-0.326544509279-0.3426633709060.0709107885740.603196885370.111466595016-0.1607185774580.6205634331680.187569612990.391763516084-0.69796858961922.715811167677.6140380709
241.61687449798-0.03031164821571.533055719051.14034704484-0.1017872868242.44809364969-0.08134196331490.474277765698-0.120520465127-0.6222995417240.1791175282160.213081607736-0.0112984147257-0.0935070260459-0.09135723321270.407712862252-0.0398681348457-0.04171123174470.503606847749-0.01797946410920.2806685135415.84912122713.3427144263680.4810149155
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 2 through 161 )
2X-RAY DIFFRACTION2chain 'A' and (resid 162 through 410 )
3X-RAY DIFFRACTION3chain 'B' and (resid 6 through 32 )
4X-RAY DIFFRACTION4chain 'B' and (resid 33 through 76 )
5X-RAY DIFFRACTION5chain 'B' and (resid 77 through 161 )
6X-RAY DIFFRACTION6chain 'B' and (resid 162 through 200 )
7X-RAY DIFFRACTION7chain 'B' and (resid 201 through 249 )
8X-RAY DIFFRACTION8chain 'B' and (resid 250 through 287 )
9X-RAY DIFFRACTION9chain 'B' and (resid 288 through 308 )
10X-RAY DIFFRACTION10chain 'B' and (resid 309 through 358 )
11X-RAY DIFFRACTION11chain 'B' and (resid 359 through 384 )
12X-RAY DIFFRACTION12chain 'B' and (resid 385 through 411 )
13X-RAY DIFFRACTION13chain 'C' and (resid 4 through 161 )
14X-RAY DIFFRACTION14chain 'C' and (resid 162 through 410 )
15X-RAY DIFFRACTION15chain 'D' and (resid 5 through 32 )
16X-RAY DIFFRACTION16chain 'D' and (resid 33 through 200 )
17X-RAY DIFFRACTION17chain 'D' and (resid 201 through 338 )
18X-RAY DIFFRACTION18chain 'D' and (resid 339 through 411 )
19X-RAY DIFFRACTION19chain 'E' and (resid 5 through 161 )
20X-RAY DIFFRACTION20chain 'E' and (resid 162 through 410 )
21X-RAY DIFFRACTION21chain 'F' and (resid 1 through 183 )
22X-RAY DIFFRACTION22chain 'F' and (resid 184 through 249 )
23X-RAY DIFFRACTION23chain 'F' and (resid 250 through 287 )
24X-RAY DIFFRACTION24chain 'F' and (resid 288 through 410 )

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