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- PDB-6y6g: lipopolysaccharide outer core galactosyltransferase WaaB apo form -
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Open data
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Basic information
Entry | Database: PDB / ID: 6y6g | ||||||
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Title | lipopolysaccharide outer core galactosyltransferase WaaB apo form | ||||||
![]() | Lipopolysaccharide 1,6-galactosyltransferase | ||||||
![]() | BIOSYNTHETIC PROTEIN / WaaB / lipopolysaccharide / galactosyltransferase / lipopolysaccharide outer core | ||||||
Function / homology | Glycosyltransferase Family 4 / Glycosyltransferase subfamily 4-like, N-terminal domain / Glycosyl transferase, family 1 / lipopolysaccharide core region biosynthetic process / Glycosyl transferases group 1 / Transferases; Glycosyltransferases; Hexosyltransferases / glycosyltransferase activity / Lipopolysaccharide 1,6-galactosyltransferase![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zhang, Z.Y. / Ashworth, G. / Wang, Z.S. / Zhu, X.F. / Dong, C.J. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural insights into the lipopolysaccharide outer core galactosyltransferase WaaB Authors: Zhang, Z.Y. / Ashworth, G. / Wang, Z.S. / Zhu, X.F. / Dong, C.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 169.2 KB | Display | ![]() |
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PDB format | ![]() | 134.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 420.2 KB | Display | ![]() |
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Full document | ![]() | 420.8 KB | Display | |
Data in XML | ![]() | 17.5 KB | Display | |
Data in CIF | ![]() | 26.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 40943.172 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: WaaB Source: (gene. exp.) ![]() Gene: rfaB, waaB, STM3719 Production host: ![]() ![]() References: UniProt: Q06994, Transferases; Glycosyltransferases; Hexosyltransferases |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.94 Å3/Da / Density % sol: 58.1 % |
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Crystal grow | Temperature: 297 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 1% tryptone, 1mM sodium azide, 0.05M HEPES pH7.0 and 20% w/v PEG 3350. |
-Data collection
Diffraction | Mean temperature: 81 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4r / Detector: CCD / Date: Feb 9, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.823 Å / Relative weight: 1 |
Reflection | Resolution: 1.81→67.54 Å / Num. obs: 44277 / % possible obs: 98.5 % / Redundancy: 60.1 % / CC1/2: 1 / Rmerge(I) obs: 0.125 / Net I/σ(I): 29 |
Reflection shell | Resolution: 1.81→1.875 Å / Redundancy: 18.2 % / Rmerge(I) obs: 0.863 / Num. unique obs: 3879 / CC1/2: 0.6 / % possible all: 86.2 |
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Processing
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Refinement | Method to determine structure: ![]() Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 106.01 Å2 / Biso mean: 30.493 Å2 / Biso min: 16.25 Å2
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Refinement step | Cycle: final / Resolution: 1.81→67.54 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.81→1.857 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Origin x: 106.3279 Å / Origin y: 130.3869 Å / Origin z: 91.3015 Å
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