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Open data
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Basic information
Entry | Database: PDB / ID: 6wme | ||||||
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Title | Human Sun2-KASH3 complex | ||||||
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![]() | STRUCTURAL PROTEIN / LINC Complex | ||||||
Function / homology | ![]() nuclear migration along microfilament / nucleokinesis involved in cell motility in cerebral cortex radial glia guided migration / nuclear matrix anchoring at nuclear membrane / cytoskeleton-nuclear membrane anchor activity / meiotic nuclear membrane microtubule tethering complex / lamin binding / establishment of protein localization to membrane / nuclear outer membrane / nuclear migration / nuclear inner membrane ...nuclear migration along microfilament / nucleokinesis involved in cell motility in cerebral cortex radial glia guided migration / nuclear matrix anchoring at nuclear membrane / cytoskeleton-nuclear membrane anchor activity / meiotic nuclear membrane microtubule tethering complex / lamin binding / establishment of protein localization to membrane / nuclear outer membrane / nuclear migration / nuclear inner membrane / centrosome localization / protein-membrane adaptor activity / rough endoplasmic reticulum / cytoskeleton organization / Meiotic synapsis / mitotic spindle organization / condensed nuclear chromosome / meiotic cell cycle / actin filament binding / nuclear envelope / regulation of cell shape / microtubule binding / nuclear membrane / membrane => GO:0016020 / chromosome, telomeric region / endosome membrane / positive regulation of cell migration / identical protein binding / membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Cruz, V.E. / Schwartz, T.U. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural Analysis of Different LINC Complexes Reveals Distinct Binding Modes. Authors: Cruz, V.E. / Esra Demircioglu, F. / Schwartz, T.U. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 130.2 KB | Display | ![]() |
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PDB format | ![]() | 84 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6wmdC ![]() 6wmfC ![]() 6wmgC ![]() 4dxtS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 22349.930 Da / Num. of mol.: 1 / Mutation: Q534D, L574I, T683G, M684R, A685G Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 3616.972 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
#3: Chemical | ChemComp-K / |
#4: Water | ChemComp-HOH / |
Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.99 Å3/Da / Density % sol: 58.9 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 16-18% PEG 3350, 0.2M magnesium chloride, 0.1M ammonium citrate, 0.1M BisTris/HCl, 0.01M nickel chloride |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Jul 31, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9792 Å / Relative weight: 1 |
Reflection | Resolution: 1.53→63.43 Å / Num. obs: 47679 / % possible obs: 99.9 % / Redundancy: 20 % / Biso Wilson estimate: 27.71 Å2 / CC1/2: 0.999 / Rpim(I) all: 0.014 / Net I/σ(I): 68.3 |
Reflection shell | Resolution: 1.53→1.57 Å / Num. unique obs: 2783 / CC1/2: 0.753 / % possible all: 82 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4DXT Resolution: 1.53→63.43 Å / SU ML: 0.1972 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 25.9494 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 39.42 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.53→63.43 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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