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- PDB-6vie: Structure of caspase-1 in complex with gasdermin D -

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Basic information

Entry
Database: PDB / ID: 6vie
TitleStructure of caspase-1 in complex with gasdermin D
Components
  • Caspase-1 subunit p10
  • Caspase-1 subunit p20
  • Gasdermin-D
KeywordsIMMUNE SYSTEM / dual site recognition
Function / homology
Function and homology information


Release of apoptotic factors from the mitochondria / Pyroptosis / Regulation of TLR by endogenous ligand / pyroptotic cell death / Interleukin-1 processing / caspase-1 / protease inhibitor complex / AIM2 inflammasome complex assembly / IPAF inflammasome complex / The AIM2 inflammasome ...Release of apoptotic factors from the mitochondria / Pyroptosis / Regulation of TLR by endogenous ligand / pyroptotic cell death / Interleukin-1 processing / caspase-1 / protease inhibitor complex / AIM2 inflammasome complex assembly / IPAF inflammasome complex / The AIM2 inflammasome / AIM2 inflammasome complex / The IPAF inflammasome / icosanoid biosynthetic process / NLRP1 inflammasome complex / canonical inflammasome complex / cytokine precursor processing / positive regulation of interleukin-18 production / pore complex assembly / CARD domain binding / NLRP3 inflammasome complex assembly / NLRP3 inflammasome complex / positive regulation of interleukin-1 production / wide pore channel activity / interleukin-18-mediated signaling pathway / plasma membrane repair / phosphatidic acid binding / ceramide biosynthetic process / Interleukin-1 processing / positive regulation of tumor necrosis factor-mediated signaling pathway / cardiolipin binding / osmosensory signaling pathway / Interleukin-37 signaling / phosphatidylinositol-4-phosphate binding / interleukin-1-mediated signaling pathway / pattern recognition receptor signaling pathway / cysteine-type endopeptidase activator activity involved in apoptotic process / signaling receptor ligand precursor processing / phosphatidylserine binding / TP53 Regulates Transcription of Caspase Activators and Caspases / cytokine binding / protein autoprocessing / pyroptotic inflammatory response / protein secretion / The NLRP3 inflammasome / Pyroptosis / Purinergic signaling in leishmaniasis infection / phosphatidylinositol-4,5-bisphosphate binding / Neutrophil degranulation / positive regulation of interleukin-1 beta production / protein maturation / cellular response to mechanical stimulus / NOD1/2 Signaling Pathway / protein homooligomerization / cellular response to type II interferon / mitochondrial membrane / kinase binding / positive regulation of inflammatory response / SARS-CoV-1 activates/modulates innate immune responses / regulation of inflammatory response / cellular response to lipopolysaccharide / endopeptidase activity / regulation of apoptotic process / defense response to virus / defense response to Gram-negative bacterium / microtubule / positive regulation of canonical NF-kappaB signal transduction / defense response to bacterium / defense response to Gram-positive bacterium / innate immune response / cysteine-type endopeptidase activity / apoptotic process / nucleolus / signal transduction / protein-containing complex / proteolysis / extracellular space / nucleoplasm / identical protein binding / membrane / plasma membrane / cytoplasm / cytosol
Similarity search - Function
Gasdermin / Gasdermin, PUB domain / Gasdermin PUB domain / Gasdermin, pore forming domain / Gasdermin pore forming domain / Caspase-like / Caspase recruitment domain / CARD domain / CARD caspase recruitment domain profile. / Caspase recruitment domain ...Gasdermin / Gasdermin, PUB domain / Gasdermin PUB domain / Gasdermin, pore forming domain / Gasdermin pore forming domain / Caspase-like / Caspase recruitment domain / CARD domain / CARD caspase recruitment domain profile. / Caspase recruitment domain / Peptidase C14 family / Peptidase family C14A, His active site / Caspase family histidine active site. / Peptidase C14, caspase non-catalytic subunit p10 / Peptidase family C14A, cysteine active site / Caspase family cysteine active site. / Caspase family p10 domain profile. / Peptidase C14A, caspase catalytic domain / Caspase, interleukin-1 beta converting enzyme (ICE) homologues / Peptidase C14, p20 domain / Caspase family p20 domain profile. / : / Caspase domain / Caspase-like domain superfamily / Death-like domain superfamily / Alpha-Beta Plaits / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
Caspase-1 / Gasdermin-D
Similarity search - Component
Biological speciesHomo sapiens (human)
Mus musculus (house mouse)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 3.4 Å
AuthorsLiu, Z. / Xiao, T.S.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM127609 United States
CitationJournal: Immunity / Year: 2020
Title: Caspase-1 Engages Full-Length Gasdermin D through Two Distinct Interfaces That Mediate Caspase Recruitment and Substrate Cleavage.
Authors: Liu, Z. / Wang, C. / Yang, J. / Chen, Y. / Zhou, B. / Abbott, D.W. / Xiao, T.S.
History
DepositionJan 13, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jul 1, 2020Provider: repository / Type: Initial release
Revision 1.1Jul 29, 2020Group: Database references / Category: citation / Item: _citation.journal_volume / _citation.page_first
Revision 1.2Oct 11, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Caspase-1 subunit p20
B: Caspase-1 subunit p10
C: Gasdermin-D
D: Gasdermin-D


Theoretical massNumber of molelcules
Total (without water)133,6024
Polymers133,6024
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area8530 Å2
ΔGint-42 kcal/mol
Surface area53670 Å2
Unit cell
Length a, b, c (Å)138.500, 202.610, 124.270
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number20
Space group name H-MC2221
Space group name HallC2c2
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-ID
11
21

NCS domain segments:

Ens-ID: 1

Dom-IDComponent-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11SERSERSERSER(chain 'C' and (resid 3 through 95 or resid 117...CC3 - 694 - 70
12VALVALVALVAL(chain 'C' and (resid 3 through 95 or resid 117...CC83 - 9584 - 96
13ALAALAILEILE(chain 'C' and (resid 3 through 95 or resid 117...CC117 - 173118 - 174
14METMETGLUGLU(chain 'C' and (resid 3 through 95 or resid 117...CC206 - 242207 - 243
15ASPASPARGARG(chain 'C' and (resid 3 through 95 or resid 117...CC288 - 451265 - 428
16VALVALGLNGLN(chain 'C' and (resid 3 through 95 or resid 117...CC454 - 484431 - 461
27SERSERSERSER(chain 'D' and (resid 3 through 451 or resid 454 through 484))DD3 - 694 - 70
28VALVALVALVAL(chain 'D' and (resid 3 through 451 or resid 454 through 484))DD83 - 9584 - 96
29ALAALAILEILE(chain 'D' and (resid 3 through 451 or resid 454 through 484))DD117 - 173118 - 174
210METMETGLUGLU(chain 'D' and (resid 3 through 451 or resid 454 through 484))DD206 - 242207 - 243
211ASPASPARGARG(chain 'D' and (resid 3 through 451 or resid 454 through 484))DD288 - 451265 - 428
212VALVALGLNGLN(chain 'D' and (resid 3 through 451 or resid 454 through 484))DD454 - 484431 - 461

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Components

#1: Protein Caspase-1 subunit p20 / CASP-1 / Interleukin-1 beta convertase / IL-1BC / Interleukin-1 beta-converting enzyme / IL-1 beta- ...CASP-1 / Interleukin-1 beta convertase / IL-1BC / Interleukin-1 beta-converting enzyme / IL-1 beta-converting enzyme / p45


Mass: 21561.842 Da / Num. of mol.: 1 / Fragment: residues 120-316 / Mutation: C285A
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: CASP1, IL1BC, IL1BCE / Production host: Escherichia coli (E. coli) / References: UniProt: P29466, caspase-1
#2: Protein Caspase-1 subunit p10 / CASP-1 / Interleukin-1 beta convertase / IL-1BC / Interleukin-1 beta-converting enzyme / IL-1 beta- ...CASP-1 / Interleukin-1 beta convertase / IL-1BC / Interleukin-1 beta-converting enzyme / IL-1 beta-converting enzyme / p45


Mass: 10402.885 Da / Num. of mol.: 1 / Fragment: residues 317-404
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: CASP1, IL1BC, IL1BCE / Production host: Escherichia coli (E. coli) / References: UniProt: P29466, caspase-1
#3: Protein Gasdermin-D / Gasdermin domain-containing protein 1


Mass: 50818.738 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Gsdmdc1, Gsdmd / Production host: Escherichia coli (E. coli) / References: UniProt: Q9D8T2

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.3 Å3/Da / Density % sol: 62.69 %
Crystal growTemperature: 277 K / Method: vapor diffusion, sitting drop / pH: 7
Details: 0.2 M Ammonium citrate tribasic pH 7.0, 20% PEG3350

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-E / Wavelength: 0.979 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jun 19, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.979 Å / Relative weight: 1
ReflectionResolution: 3.4→78.52 Å / Num. obs: 24394 / % possible obs: 99.9 % / Redundancy: 6.906 % / Biso Wilson estimate: 151.63 Å2 / CC1/2: 1 / Rmerge(I) obs: 0.067 / Rpim(I) all: 0.028 / Rrim(I) all: 0.073 / Χ2: 1.082 / Net I/σ(I): 16.69
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. unique obsCC1/2Rpim(I) allRrim(I) all% possible all
3.4-3.496.9062.07117680.40.8532.2499.3
3.49-3.586.821.4191.5217080.5381.53899.8
3.58-3.696.3071.0042.1317130.7111.09699.9
3.69-3.87.3390.7943.0316320.8320.854100
3.8-3.937.3460.6053.9815960.910.651100
3.93-4.067.3160.425.4315460.9540.452100
4.06-4.227.2570.2857.8314910.9790.30799.9
4.22-4.397.0290.18111.5214380.9910.196100
4.39-4.587.1410.1381513850.9940.149100
4.58-4.817.0130.10718.4213130.9960.11599.9
4.81-5.076.8360.10119.0712580.9950.1199.9
5.07-5.386.2320.09718.7612100.9940.10699.9
5.38-5.756.8770.08721.8511110.9960.094100
5.75-6.217.270.07126.1410550.9980.07799.9
6.21-6.87.0910.05433.919720.9980.058100
6.8-7.66.7590.04340.78850.9990.046100
7.6-8.786.6570.02953.697940.9990.03199.9
8.78-10.755.810.02359.656680.9990.02599.4
10.75-15.216.5060.02167.275340.9990.023100
15.21-78.525.6470.0264.1531710.02298.8

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Phasing

PhasingMethod: molecular replacement
Phasing MR
Highest resolutionLowest resolution
Rotation3.5 Å114.34 Å
Translation3.5 Å114.34 Å

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Processing

Software
NameVersionClassification
PHENIX1.17.1_3660refinement
XDSdata reduction
XSCALEdata scaling
PHASER2.8.3phasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6N9N,6BZ9

6n9n

6bz9


Resolution: 3.4→78.52 Å / SU ML: 0.5585 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 33.834
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.3143 1237 5.07 %
Rwork0.2877 23141 -
obs0.289 24378 99.82 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 190.89 Å2
Refinement stepCycle: LAST / Resolution: 3.4→78.52 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms8038 0 0 0 8038
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00298171
X-RAY DIFFRACTIONf_angle_d0.781311060
X-RAY DIFFRACTIONf_chiral_restr0.04421294
X-RAY DIFFRACTIONf_plane_restr0.00441415
X-RAY DIFFRACTIONf_dihedral_angle_d20.48031078
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.4-3.540.39031390.36752523X-RAY DIFFRACTION99.29
3.54-3.70.37111320.35162525X-RAY DIFFRACTION99.85
3.7-3.890.31541370.33452548X-RAY DIFFRACTION99.93
3.89-4.140.3241230.31112554X-RAY DIFFRACTION100
4.14-4.460.27981380.27432562X-RAY DIFFRACTION99.96
4.46-4.90.30181310.2642563X-RAY DIFFRACTION99.93
4.9-5.610.31881500.30742575X-RAY DIFFRACTION99.93
5.61-7.070.35941440.3292586X-RAY DIFFRACTION99.96
7.07-78.520.29271430.25652705X-RAY DIFFRACTION99.58
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
19.703261157171.465516498992.806580650032.51646850139-3.7377374838.50066338747-1.65690796161-3.46543343472-1.20810295382-1.37568687610.380585736825-0.5726938860362.69875032528-2.74424239759-0.4059259620791.831197397410.476270960916-0.544582630991.09100623908-0.3478863141471.6735777166111.2186697821-7.80446981275-35.1504558508
22.01211492047-0.383858908637-2.261579244379.034952180232.633117011019.202094092510.367267730593-0.3570901148020.641341689675-0.05484436876610.166875444259-2.429681932741.59425391.42101470238-0.5857029479691.242382590620.3206560358190.2680697008592.030578530380.2290519056511.3976273869618.66992784651.57281170816-41.7472193797
38.895428471131.35979372805-0.4108469100526.077124748653.522298339082.228377376491.23740602993-2.29388807694-0.609466297582.46058722923-0.503457299528-1.474994837820.729986786698-0.224008793179-0.5520088866471.21178028367-0.382038840784-0.1239759309911.800415037480.09529702589581.2898152514222.167787757718.6721074635-22.4481185981
46.647614231151.048636355792.752792882442.425854494272.307153190117.263960126180.587228523664-0.634972446731.318349266090.789072427044-1.24665122459-0.164942780201-1.04720652052.415822999830.2820931193181.3017947083-0.55589986661-0.08317643778981.965466086720.4872171816681.2863118074723.911812408917.0427752503-30.502447572
53.425291668470.650123972112-1.305858317927.30400203344-3.327463662516.88076892578-0.101738122459-0.0274762305374-0.442097502097-0.718972408413-0.970097025435-2.411393232790.2533400802052.42891388591.044101098590.884325877706-0.1462125456930.1223648005311.689253103940.3983406459341.1302116257720.001257659518.674357565-37.7430663787
63.28554474542.14338736979-3.829102466381.62384254315-2.766552041434.848315842840.9651349972750.484085726807-0.3963748498072.18946324250.670265043347-2.39178237993-1.322692426111.20152356929-1.631352059671.60406129066-0.397781150995-0.1408852356891.29890415422-0.05628582757972.0594357790112.851559901932.8294285191-23.599356847
74.513538166673.88496441883.645784559043.491754315773.527932419783.714996340030.5644087981080.196213359857-0.756738495077-1.262328396890.3003233456310.257077085551-0.4914335213740.7476927038041.511966148013.72172080552-1.97482606409-1.607283577850.700692940867-0.4759434646810.2469349793519.3506471911625.5605870492-34.8606637164
88.83748676538-3.96649974229-0.3874430645266.021687545985.666519972267.304595608322.604572017870.8670444341471.42955862944-1.31271474276-0.327307803869-2.18755221084-1.64726397709-2.25100003499-1.65666701741.14755585818-0.005129893896670.2625145606281.043743053770.01989327375161.395140220679.3536922482318.1359379259-32.9793885128
92.642195745642.12389783586-0.8977235387019.67321384527-3.08755015310.9369343432470.4385818457080.0364139791513-0.731612806355-0.6803265144190.7117999901191.251189882631.617452569082.344731530510.370352524612.90499560696-0.715822262587-0.1270148307191.991581650250.3295933264271.07646593072-13.883604881510.1672170768-0.0272464221998
108.04903348206-0.0149742187617-3.573386416662.53114952206-2.83957528974.80415120441-0.306204562536-1.55904975154-2.966880801720.347986685526-0.926004035652.08174465441-0.552167352982-0.5984367909920.3792608128481.15153863624-0.313687944366-0.5024371442251.357244142380.4453781642391.20757377893-17.63345399148.1199343651117.1168632663
116.850070549670.513586620032-0.4732761082574.545265878580.770902334394.322720509770.258103590628-1.128215464070.708185899740.420073254466-0.294435694337-0.1960805287330.117771692480.28875710092-0.022022129420.946159417995-0.270572686855-0.0607609719891.16135515253-0.001909574433421.502359365489.8435881768812.7898995641-26.5470440517
127.31353697987-5.30695249346-5.834727910864.061703567975.311830030151.99035194851-0.3415129133820.482809143974-0.355198357230.293167410302-0.3732460045822.820148166962.59206954287-2.89938563042-0.4021573193180.478157667711-0.120530418087-0.272299419661.52481794947-0.143506998921.802190943294.908077524443.9767423087-25.2967479107
130.82321384167-0.988364275133-0.08186573101675.96638713751-0.7259922231037.439635108610.695317150644-1.30290537953-0.4623133966280.407683915562-0.0481981512151-0.2732263394490.1281782822331.11902750895-0.4548532195470.581826759875-0.299823606068-0.09204445981151.565227876660.2738405955171.343600745216.201357875098.45561503139-26.6534513949
147.022318726472.90411731083-3.379607890328.38505064933-0.7422274838087.354947159610.904463397196-2.169509798642.20379736482.448848436250.110297206481-0.691369499742-1.64559784493-1.04743989186-1.395709588671.519850225430.2826721764320.2450242338781.624889547230.08620833064181.7085675434315.868386705847.48980088824.873916382
157.01672389932-1.59393515004-1.266439801718.57292926765-1.007495151069.309434233460.1721469511530.4490486145080.3098930295620.738774008045-0.638052533283-1.166907871010.3220390776940.8249734470860.4644818838711.07062376043-0.193716738464-0.1791885760411.50204220990.04564987303361.4349269400721.785093783736.576204619622.9382753237
163.410879364361.263700628130.2835389613721.217959398261.461397121671.79063890364-0.5691395717722.681215455830.418459567604-0.03708792017390.659978154406-0.405357305986-0.0107431456291-0.126628176824-0.2957020276151.8263922246-0.4789099568370.5591712851592.84730184308-0.1515934786161.440914730039.3362415801330.2141188142-3.33507631355
174.442315121091.61133867703-0.7309346644135.791197575094.605633994114.283199806350.259255530738-1.23980516954-0.5208485600512.05155695681-0.5340303442580.7478435140010.0219441318509-0.1559488210430.0549279926841.78497373338-0.07851662727050.1076524116141.925921582780.07327172958491.00061559445-3.4970656537325.20761192338.07815832316
183.15044229207-2.629612458591.650456884968.18905965842-1.852446571614.11469523606-0.2891531508770.2093413558250.154703475861.918610067890.3213663132740.651803566476-3.48472210222-0.5964045144090.002963169269612.51172246223-0.1138817814910.4528733313421.5691111709-0.2830659993811.71939728481-2.5545353237550.72473284225.13920937745
192.90051424678-0.9500671968411.582582144833.697821897122.277724442093.072811657360.5455615711780.0285909579564-0.3338963307540.0938011956966-0.6965776228130.5396661758110.09209576720470.121957716376-0.2197802436291.10015438869-0.2431120214760.01714138578822.04244928813-0.05015773849541.3363816854937.632579739463.279106821313.8643379353
202.45794688913-2.263480132220.7550540809128.664598207172.515611193433.57114741478-0.230892387893-0.3804949489450.02908864802060.655899718261-0.4692807192730.894525704132-0.699389104203-0.6800723441740.8258696425481.68151676309-0.84101705876-0.2377192996511.84880211498-0.1645172164281.4896936505231.555596086177.002656646718.4170564167
212.623689553920.3655720359312.740319667888.95501157251.676645333187.363864928920.0383426460932-1.285335161780.740862599360.7354011874510.6237956789080.251695984154-1.5020799683-0.3267645288590.4607047463521.34550230841-0.9249895926530.2893896412082.1351754236-0.4746650043971.3243935005431.918512243771.19960951215.5485573529
222.864148316830.0378494898179-2.740037354745.464222619950.5537157811312.45186824044-1.07878134868-0.551535640056-1.04155769382-1.088240690480.892670885742-0.816125973498-0.22211245709-0.279337299310.1885171376732.0548810587-0.592279629573-0.2754571730391.80299209043-0.3202837863291.1138212706133.207259959378.0148588794-13.116893213
234.28866647105-0.287334470313-2.849594717564.91363391284-4.655679723941.99359921906-2.11012323551.64438689899-0.260207654480.922157441585-1.72765332937-1.255753109313.20115185404-0.2046777819880.006562256300292.70684263925-1.69937491769-1.130121940140.350366350650.3320931223053.1017888402726.640949861852.1705023093-10.8203667633
244.620540938760.0463501973257-1.429670187784.673849873941.022205214073.75600981658-0.959689832845-0.217446724213-1.27647995024-0.5612593696630.166742530798-1.324695756930.717644358464-2.857921982361.231379783342.22804004963-0.6826969118190.7572122756871.769177884810.03886804592382.1578324612233.482736163658.7713853055-10.4106451847
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 131 through 151 )
2X-RAY DIFFRACTION2chain 'A' and (resid 152 through 163 )
3X-RAY DIFFRACTION3chain 'A' and (resid 164 through 195 )
4X-RAY DIFFRACTION4chain 'A' and (resid 196 through 207 )
5X-RAY DIFFRACTION5chain 'A' and (resid 208 through 235 )
6X-RAY DIFFRACTION6chain 'A' and (resid 236 through 254 )
7X-RAY DIFFRACTION7chain 'A' and (resid 255 through 264 )
8X-RAY DIFFRACTION8chain 'A' and (resid 265 through 293 )
9X-RAY DIFFRACTION9chain 'A' and (resid 294 through 307 )
10X-RAY DIFFRACTION10chain 'A' and (resid 308 through 316 )
11X-RAY DIFFRACTION11chain 'B' and (resid 317 through 362 )
12X-RAY DIFFRACTION12chain 'B' and (resid 363 through 376 )
13X-RAY DIFFRACTION13chain 'B' and (resid 377 through 404 )
14X-RAY DIFFRACTION14chain 'C' and (resid 3 through 39 )
15X-RAY DIFFRACTION15chain 'C' and (resid 40 through 236 )
16X-RAY DIFFRACTION16chain 'C' and (resid 237 through 307 )
17X-RAY DIFFRACTION17chain 'C' and (resid 308 through 382 )
18X-RAY DIFFRACTION18chain 'C' and (resid 383 through 484 )
19X-RAY DIFFRACTION19chain 'D' and (resid 2 through 39 )
20X-RAY DIFFRACTION20chain 'D' and (resid 40 through 156 )
21X-RAY DIFFRACTION21chain 'D' and (resid 157 through 289 )
22X-RAY DIFFRACTION22chain 'D' and (resid 290 through 414 )
23X-RAY DIFFRACTION23chain 'D' and (resid 415 through 434 )
24X-RAY DIFFRACTION24chain 'D' and (resid 435 through 484 )

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