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- PDB-5uak: Dephosphorylated, ATP-free human cystic fibrosis transmembrane co... -

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Basic information

Entry
Database: PDB / ID: 5uak
TitleDephosphorylated, ATP-free human cystic fibrosis transmembrane conductance regulator (CFTR)
DescriptorCystic fibrosis transmembrane conductance regulator
R domain of humanCFTR
KeywordsMEMBRANE PROTEIN / HYDROLASE / ABC transporter / anion channel / cystic fibrosis / membrane protein / hydrolase
Specimen sourceHomo sapiens / human
MethodElectron microscopy (3.87 A resolution / Single particle)
AuthorsLiu, F. / Zhang, Z. / Chen, J.
CitationCell, 2017, 169, 85-95.e8

Cell, 2017, 169, 85-95.e8 StrPapers
Molecular Structure of the Human CFTR Ion Channel.
Fangyu Liu / Zhe Zhang / László Csanády / David C Gadsby / Jue Chen

DateDeposition: Dec 19, 2016 / Release: Jan 18, 2017 / Last modification: Apr 12, 2017

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Assembly

Deposited unit
A: Cystic fibrosis transmembrane conductance regulator
R: Cystic fibrosis transmembrane conductance regulator


Theoretical massNumber of molelcules
Total (without water)170,9892
Polyers170,9892
Non-polymers00
Water0
#1


TypeNameSymmetry operationNumber
identity operation1_5551

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Components

#1: Polypeptide(L)Cystic fibrosis transmembrane conductance regulator / CFTR / ATP-binding cassette sub-family C member 7 / Channel conductance-controlling ATPase / cAMP-dependent chloride channel


Mass: 169353.578 Da / Num. of mol.: 1 / Source: (gene. exp.) Homo sapiens / human / References: UniProt: P13569, EC: 3.6.3.49

Cellular component

Molecular function

Biological process

#2: Polypeptide(L)Cystic fibrosis transmembrane conductance regulator / CFTR / ATP-binding cassette sub-family C member 7 / Channel conductance-controlling ATPase / cAMP-dependent chloride channel


Mass: 1635.006 Da / Num. of mol.: 1 / Fragment: R domain / Source: (gene. exp.) Homo sapiens / human

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentReconstruction method: SINGLE PARTICLE

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Sample preparation

ComponentName: human cystic fibrosis transmembrane conductance regulator (CFTR)
Type: COMPLEX
VitrificationInstrument: FEI VITROBOT MARK I / Cryogen name: ETHANE / Humidity: 100 %

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyMicroscope model: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD

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Processing

3D reconstructionResolution: 3.87 / Resolution method: FSC 0.143 CUT-OFF / Number of particles: 415915

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