+Open data
-Basic information
Entry | Database: PDB / ID: 3ex7 | ||||||
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Title | The crystal structure of EJC in its transition state | ||||||
Components |
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Keywords | hydrolase/RNA binding protein/RNA / PROTEIN-RNA COMPLEX / mRNA processing / mRNA splicing / mRNA transport / Nonsense-mediated mRNA decay / Nucleus / RNA-binding / Spliceosome / Transport / Alternative splicing / Cytoplasm / Phosphoprotein / Acetylation / ATP-binding / Helicase / Hydrolase / Nucleotide-binding / rRNA processing / Coiled coil / hydrolase-RNA binding protein-RNA COMPLEX | ||||||
Function / homology | Function and homology information exon-exon junction subcomplex mago-y14 / negative regulation of selenocysteine incorporation / regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / cellular response to selenite ion / selenocysteine insertion sequence binding / exon-exon junction complex / intracellular mRNA localization / regulation of translation at postsynapse, modulating synaptic transmission / negative regulation of excitatory postsynaptic potential / Z-decay: degradation of maternal mRNAs by zygotically expressed factors ...exon-exon junction subcomplex mago-y14 / negative regulation of selenocysteine incorporation / regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / cellular response to selenite ion / selenocysteine insertion sequence binding / exon-exon junction complex / intracellular mRNA localization / regulation of translation at postsynapse, modulating synaptic transmission / negative regulation of excitatory postsynaptic potential / Z-decay: degradation of maternal mRNAs by zygotically expressed factors / regulation of mRNA processing / Deadenylation of mRNA / U2-type catalytic step 1 spliceosome / poly(A) binding / M-decay: degradation of maternal mRNAs by maternally stored factors / RNA stem-loop binding / mRNA 3'-end processing / embryonic cranial skeleton morphogenesis / Transport of Mature mRNA derived from an Intron-Containing Transcript / RNA Polymerase II Transcription Termination / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / exploration behavior / regulation of alternative mRNA splicing, via spliceosome / associative learning / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / ribonucleoprotein complex binding / mRNA export from nucleus / cellular response to brain-derived neurotrophic factor stimulus / catalytic step 2 spliceosome / mRNA Splicing - Major Pathway / RNA splicing / positive regulation of translation / response to organic cyclic compound / ISG15 antiviral mechanism / mRNA splicing, via spliceosome / rRNA processing / Regulation of expression of SLITs and ROBOs / cytoplasmic stress granule / regulation of translation / postsynapse / nuclear membrane / negative regulation of translation / RNA helicase activity / RNA helicase / nuclear speck / mRNA binding / dendrite / neuronal cell body / glutamatergic synapse / ubiquitin protein ligase binding / nucleolus / perinuclear region of cytoplasm / enzyme binding / ATP hydrolysis activity / RNA binding / nucleoplasm / ATP binding / membrane / identical protein binding / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.301 Å | ||||||
Authors | Andersen, G.R. / Nielsen, K.H. | ||||||
Citation | Journal: Rna / Year: 2009 Title: Mechanism of ATP turnover inhibition in the EJC Authors: Nielsen, K.H. / Chamieh, H. / Andersen, C.B. / Fredslund, F. / Hamborg, K. / Le Hir, H. / Andersen, G.R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3ex7.cif.gz | 590.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3ex7.ent.gz | 482.9 KB | Display | PDB format |
PDBx/mmJSON format | 3ex7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ex/3ex7 ftp://data.pdbj.org/pub/pdb/validation_reports/ex/3ex7 | HTTPS FTP |
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-Related structure data
Related structure data | 2hyiS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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-Components
-Protein , 4 types, 8 molecules AEBGCHDI
#1: Protein | Mass: 17189.625 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: MAGOH / Plasmid: pET30 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: P61326 #2: Protein | Mass: 14637.176 Da / Num. of mol.: 2 / Fragment: UNP residues 51-174 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: RBM8A / Plasmid: pET30 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: Q9Y5S9 #3: Protein | Mass: 47173.230 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: EIF4A3 / Plasmid: pET30 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 References: UniProt: P38919, Hydrolases; Acting on acid anhydrides; In phosphorus-containing anhydrides #4: Protein | Mass: 17275.068 Da / Num. of mol.: 2 / Fragment: UNP residues 138-283 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CASC3 / Plasmid: pET30 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: O15234 |
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-RNA chain , 1 types, 2 molecules FJ
#5: RNA chain | Mass: 1792.037 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: poly Uracil (Sigma) |
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-Non-polymers , 4 types, 386 molecules
#6: Chemical | #7: Chemical | #8: Chemical | #9: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.16 Å3/Da / Density % sol: 61.08 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 8.8 Details: 7% PEG 3350, 50mM Tris HCl pH 8.8, 200mM NaAcetate, VAPOR DIFFUSION, HANGING DROP, temperature 277K | ||||||||||||||||||||||||||||||||||||
Components of the solutions |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID23-1 / Wavelength: 0.91745 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Sep 19, 2006 |
Radiation | Monochromator: Si111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.91745 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→40 Å / Num. all: 108638 / Num. obs: 107635 / % possible obs: 99.1 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 4.6 % / Biso Wilson estimate: 34.55 Å2 / Rsym value: 0.108 / Net I/σ(I): 11.35 |
Reflection shell | Resolution: 2.3→2.4 Å / Redundancy: 4.6 % / Mean I/σ(I) obs: 2.58 / Num. unique all: 12443 / Rsym value: 0.666 / % possible all: 96 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2HYI Resolution: 2.301→39.065 Å / Occupancy max: 1 / Occupancy min: 1 / FOM work R set: 0.804 / SU ML: 0.34 / Cross valid method: Througout / σ(F): 0 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 57.702 Å2 / ksol: 0.354 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 275.05 Å2 / Biso mean: 67.293 Å2 / Biso min: 12.96 Å2
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Refinement step | Cycle: LAST / Resolution: 2.301→39.065 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 15
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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