[English] 日本語
Yorodumi- PDB-6t51: NMR structure of KRAS22RT G-quadruplex forming within KRAS promot... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6t51 | ||||||
---|---|---|---|---|---|---|---|
Title | NMR structure of KRAS22RT G-quadruplex forming within KRAS promoter region at physological temperature | ||||||
Components | KRAS22RT | ||||||
Keywords | DNA / KRAS oncogene / promoter region / G-quadruplex | ||||||
Function / homology | : / DNA / DNA (> 10) Function and homology information | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / molecular dynamics | ||||||
Authors | Marquevielle, J. / Salgado, G. | ||||||
Citation | Journal: Biomol.Nmr Assign. / Year: 2018 Title: 1H,13C, and15N chemical shift assignments of a G-quadruplex forming sequence within the KRAS proto-oncogene promoter region. Authors: Marquevielle, J. / Kumar, M.V.V. / Mergny, J.L. / Salgado, G.F. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 6t51.cif.gz | 166.6 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb6t51.ent.gz | 122.8 KB | Display | PDB format |
PDBx/mmJSON format | 6t51.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/t5/6t51 ftp://data.pdbj.org/pub/pdb/validation_reports/t5/6t51 | HTTPS FTP |
---|
-Related structure data
Similar structure data | |
---|---|
Other databases |
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: DNA chain | Mass: 6986.514 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) | ||
---|---|---|---|
#2: Chemical | Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Details |
| |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample |
| |||||||||||||||
Sample conditions | Ionic strength: 50 KCl; 10KPi mM / Label: KRAS22RT / pH: 6.66 / Pressure: 1 atm / Temperature: 310 K |
-NMR measurement
NMR spectrometer |
|
---|
-Processing
NMR software |
| ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: molecular dynamics / Software ordinal: 1 | ||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 750 / Conformers submitted total number: 10 |