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- PDB-6t00: Crystal structure of Cold Shock Protein B (CSP-B) containing 4-F-... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6t00 | ||||||
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Title | Crystal structure of Cold Shock Protein B (CSP-B) containing 4-F-Phe modified residues | ||||||
![]() | Cold shock protein CspD | ||||||
![]() | STRUCTURAL PROTEIN / fluorine cold shock protein | ||||||
Function / homology | ![]() nucleoid / regulation of gene expression / nucleic acid binding / DNA binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zhou, T. / Mayans, O. | ||||||
![]() | ![]() Title: What does fluorine do to a protein? Thermodynamic, and highly-resolved structural insights into fluorine-labelled variants of the cold shock protein. Authors: Welte, H. / Zhou, T. / Mihajlenko, X. / Mayans, O. / Kovermann, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 48.6 KB | Display | ![]() |
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PDB format | ![]() | 28.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6szzC ![]() 1cspS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 7426.987 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: B4122_0412, B4417_4327, CJ481_22435, DFO69_3150, ETA10_05025, ETK61_05130, ETL41_18150, FA024_03420, SC09_Contig19orf00064 Production host: ![]() ![]() |
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#2: Chemical | ChemComp-NHE / |
#3: Chemical | ChemComp-GOL / |
#4: Water | ChemComp-HOH / |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.93 Å3/Da / Density % sol: 58.05 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop Details: 100 mM CHES/ Sodium hydroxide pH 9.5, 1000 mM Sodium citrate tribasic |
-Data collection
Diffraction | Mean temperature: 80 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jul 12, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→39.79 Å / Num. obs: 5551 / % possible obs: 99.9 % / Redundancy: 12.43 % / CC1/2: 0.999 / Rsym value: 0.087 / Net I/σ(I): 17.23 |
Reflection shell | Resolution: 2.1→2.2 Å / Num. unique obs: 692 / CC1/2: 0.429 / Rsym value: 2.252 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1csp Resolution: 2.1→39.79 Å / SU ML: 0.2781 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 18.9329
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 63.45 Å2 | ||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→39.79 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: -7.78124837802 Å / Origin y: 14.6094842513 Å / Origin z: -2.47862220803 Å
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Refinement TLS group | Selection details: (chain 'A' and resid 1 through 67) |