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- PDB-6s9o: Designed Armadillo Repeat protein internal Lock1 fused to target ... -

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Basic information

Entry
Database: PDB / ID: 6s9o
TitleDesigned Armadillo Repeat protein internal Lock1 fused to target peptide KRKRKLKFKR
Componentsdesigned Armadillo repeat protein with internal Lock1 fused to target peptide KRKRKLKFKR
KeywordsDE NOVO PROTEIN / peptide binder / repeat protein / designed armadillo repeat protein
Biological speciessynthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.17 Å
AuthorsErnst, P. / Zosel, F. / Reichen, C. / Schuler, B. / Pluckthun, A.
CitationJournal: Acs Chem.Biol. / Year: 2020
Title: Structure-Guided Design of a Peptide Lock for Modular Peptide Binders.
Authors: Ernst, P. / Zosel, F. / Reichen, C. / Nettels, D. / Schuler, B. / Pluckthun, A.
History
DepositionJul 15, 2019Deposition site: PDBE / Processing site: PDBE
Revision 1.0Feb 19, 2020Provider: repository / Type: Initial release
Revision 1.1Mar 4, 2020Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.title

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: designed Armadillo repeat protein with internal Lock1 fused to target peptide KRKRKLKFKR
B: designed Armadillo repeat protein with internal Lock1 fused to target peptide KRKRKLKFKR
C: designed Armadillo repeat protein with internal Lock1 fused to target peptide KRKRKLKFKR
D: designed Armadillo repeat protein with internal Lock1 fused to target peptide KRKRKLKFKR
E: designed Armadillo repeat protein with internal Lock1 fused to target peptide KRKRKLKFKR
F: designed Armadillo repeat protein with internal Lock1 fused to target peptide KRKRKLKFKR
hetero molecules


Theoretical massNumber of molelcules
Total (without water)217,11432
Polymers215,8526
Non-polymers1,26226
Water39622
1
A: designed Armadillo repeat protein with internal Lock1 fused to target peptide KRKRKLKFKR
hetero molecules


Theoretical massNumber of molelcules
Total (without water)36,3008
Polymers35,9751
Non-polymers3257
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: designed Armadillo repeat protein with internal Lock1 fused to target peptide KRKRKLKFKR
hetero molecules


Theoretical massNumber of molelcules
Total (without water)36,1624
Polymers35,9751
Non-polymers1863
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: designed Armadillo repeat protein with internal Lock1 fused to target peptide KRKRKLKFKR
hetero molecules


Theoretical massNumber of molelcules
Total (without water)36,1805
Polymers35,9751
Non-polymers2044
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
D: designed Armadillo repeat protein with internal Lock1 fused to target peptide KRKRKLKFKR
hetero molecules


Theoretical massNumber of molelcules
Total (without water)36,0964
Polymers35,9751
Non-polymers1203
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
5
E: designed Armadillo repeat protein with internal Lock1 fused to target peptide KRKRKLKFKR
hetero molecules


Theoretical massNumber of molelcules
Total (without water)36,3629
Polymers35,9751
Non-polymers3878
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
6
F: designed Armadillo repeat protein with internal Lock1 fused to target peptide KRKRKLKFKR
hetero molecules


Theoretical massNumber of molelcules
Total (without water)36,0152
Polymers35,9751
Non-polymers401
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)168.700, 82.400, 191.780
Angle α, β, γ (deg.)90.000, 90.360, 90.000
Int Tables number5
Space group name H-MC121
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and (resid 11 through 74 or resid 76...
21(chain B and (resid 11 through 74 or resid 76...
31(chain C and (resid 11 through 74 or resid 76...
41(chain D and (resid 11 through 74 or resid 76...
51(chain E and (resid 11 through 74 or resid 76...
61(chain F and (resid 11 through 74 or resid 76...

NCS domain segments:
Dom-IDComponent-IDEns-IDSelection detailsAuth asym-IDAuth seq-ID
111(chain A and (resid 11 through 74 or resid 76...A11 - 74
121(chain A and (resid 11 through 74 or resid 76...A76 - 161
131(chain A and (resid 11 through 74 or resid 76...A163 - 203
141(chain A and (resid 11 through 74 or resid 76...A9 - 351
151(chain A and (resid 11 through 74 or resid 76...A247 - 332
161(chain A and (resid 11 through 74 or resid 76...A342 - 351
211(chain B and (resid 11 through 74 or resid 76...B11 - 74
221(chain B and (resid 11 through 74 or resid 76...B76 - 161
231(chain B and (resid 11 through 74 or resid 76...B163 - 203
241(chain B and (resid 11 through 74 or resid 76...B11 - 351
251(chain B and (resid 11 through 74 or resid 76...B247 - 332
261(chain B and (resid 11 through 74 or resid 76...B342 - 351
311(chain C and (resid 11 through 74 or resid 76...C11 - 74
321(chain C and (resid 11 through 74 or resid 76...C76 - 161
331(chain C and (resid 11 through 74 or resid 76...C163 - 203
341(chain C and (resid 11 through 74 or resid 76...C205 - 245
351(chain C and (resid 11 through 74 or resid 76...C247 - 351
411(chain D and (resid 11 through 74 or resid 76...D11 - 74
421(chain D and (resid 11 through 74 or resid 76...D76 - 161
431(chain D and (resid 11 through 74 or resid 76...D163 - 203
441(chain D and (resid 11 through 74 or resid 76...D205 - 245
451(chain D and (resid 11 through 74 or resid 76...D11 - 351
511(chain E and (resid 11 through 74 or resid 76...E11 - 74
521(chain E and (resid 11 through 74 or resid 76...E76 - 161
531(chain E and (resid 11 through 74 or resid 76...E163 - 203
541(chain E and (resid 11 through 74 or resid 76...E205 - 245
551(chain E and (resid 11 through 74 or resid 76...E11 - 351
611(chain F and (resid 11 through 74 or resid 76...F11 - 74
621(chain F and (resid 11 through 74 or resid 76...F76 - 161
631(chain F and (resid 11 through 74 or resid 76...F163 - 203
641(chain F and (resid 11 through 74 or resid 76...F205 - 245
651(chain F and (resid 11 through 74 or resid 76...F9 - 351

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Components

#1: Protein
designed Armadillo repeat protein with internal Lock1 fused to target peptide KRKRKLKFKR


Mass: 35975.371 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) synthetic construct (others) / Production host: Escherichia coli (E. coli)
#2: Chemical
ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 16 / Source method: obtained synthetically / Formula: Ca
#3: Chemical
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL / Ethylene glycol


Mass: 62.068 Da / Num. of mol.: 10 / Source method: obtained synthetically / Formula: C2H6O2
#4: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 22 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.12 Å3/Da / Density % sol: 60.59 %
Crystal growTemperature: 277.14 K / Method: vapor diffusion
Details: 25 % PEG smear low 0.04 M CaCl2, 0.04 M Na-formate 0.1 M Tris pH 8

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X06SA / Wavelength: 0.999998 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Nov 6, 2017
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.999998 Å / Relative weight: 1
ReflectionResolution: 3.17→48.453 Å / Num. obs: 44419 / % possible obs: 98.5 % / Redundancy: 6.918 % / Biso Wilson estimate: 69.217 Å2 / CC1/2: 0.993 / Rmerge(I) obs: 0.307 / Rrim(I) all: 0.332 / Χ2: 0.86 / Net I/σ(I): 5.82 / Num. measured all: 307274 / Scaling rejects: 101
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. measured obsNum. possibleNum. unique obsCC1/2Rrim(I) all% possible all
3.17-3.257.0341.9350.9523220333033010.7932.0999.1
3.25-3.347.0351.8550.9922336321231750.7332.00498.8
3.34-3.446.9921.7191.1221534313430800.7781.85898.3
3.44-3.546.8371.3161.4520470304629940.7851.42598.3
3.54-3.666.7211.0091.8919477294828980.8671.09498.3
3.66-3.796.5650.8082.4418422286628060.8950.87897.9
3.79-3.936.0690.6492.9616338279026920.9460.7196.5
3.93-4.095.5510.5353.3813821265824900.9490.5993.7
4.09-4.276.5530.4044.6416539256325240.9770.43898.5
4.27-4.487.4580.2866.8817937241524050.9880.30799.6
4.48-4.737.4430.2417.8317118232123000.990.2699.1
4.73-5.017.4240.2517.516289220321940.9910.2799.6
5.01-5.367.4460.3146.3915554209020890.9840.337100
5.36-5.797.4510.316.7414387193119310.9830.333100
5.79-6.347.3890.2717.713211178817880.9810.291100
6.34-7.097.2980.17411.3311874162716270.990.187100
7.09-8.197.230.08717.9410360143314330.9980.094100
8.19-10.027.0730.05924.458544122112080.9980.06498.9
10.02-14.186.8850.04827.3365419649500.9990.05298.5
14.18-48.4536.1840.0525.0533025525340.9990.05596.7

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Processing

Software
NameVersionClassification
PHENIX1.15.2_3472refinement
XSCALEdata scaling
PDB_EXTRACT3.25data extraction
XDSdata reduction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.17→48.453 Å / SU ML: 0.5 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 35.08
RfactorNum. reflection% reflection
Rfree0.2915 2211 5 %
Rwork0.2349 --
obs0.2378 44262 98.19 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 322.64 Å2 / Biso mean: 91.4222 Å2 / Biso min: 21.39 Å2
Refinement stepCycle: final / Resolution: 3.17→48.453 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms15023 0 116 22 15161
Biso mean--89.71 34.09 -
Num. residues----2034
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A8958X-RAY DIFFRACTION11.62TORSIONAL
12B8958X-RAY DIFFRACTION11.62TORSIONAL
13C8958X-RAY DIFFRACTION11.62TORSIONAL
14D8958X-RAY DIFFRACTION11.62TORSIONAL
15E8958X-RAY DIFFRACTION11.62TORSIONAL
16F8958X-RAY DIFFRACTION11.62TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
3.1701-3.2390.37411390.3505263798
3.239-3.31440.38761360.3444256698
3.3144-3.39720.3891330.3367258798
3.3972-3.48910.38911390.336263598
3.4891-3.59170.35191370.2978259098
3.5917-3.70760.31791370.2879259398
3.7076-3.840.36891370.2708260197
3.84-3.99370.34271320.2557250194
3.9937-4.17540.31151340.2529253995
4.1754-4.39540.27311390.2179265399
4.3954-4.67060.2511410.2019267499
4.6706-5.03080.28411380.203263199
5.0308-5.53650.33571420.24052710100
5.5365-6.33610.30231420.23442679100
6.3361-7.9770.26231420.19942709100
7.977-48.4530.1931430.1624274698
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
16.28212.4369-1.16865.1649-3.62422.63650.9905-0.0750.89341.0827-0.86310.2764-0.77120.76830.10530.9983-0.23380.19940.8691-0.04270.5711-47.3238-54.988440.9695
26.78532.4479-1.08077.4528-1.56714.8832-0.1516-0.26-0.46480.20080.5320.78290.58710.11180.14080.6509-0.07980.11090.89980.02130.359-46.4961-52.959130.978
37.07022.2815-2.01558.4752-1.82093.23550.35110.12750.3229-0.7115-0.1083-0.3823-0.9246-0.4402-0.00520.56270.2271-0.07560.65030.02970.3861-43.1299-44.746428.6778
44.0488-0.96-3.04482.4776-0.29383.29240.02830.16010.1349-0.26070.1608-0.0071-0.77120.1026-0.16830.74510.0328-0.00310.4039-0.01790.4172-30.8531-34.570515.8257
55.5705-1.4293-1.77932.65130.72844.11630.26930.16310.0535-0.4603-0.2268-0.1969-0.49980.06-0.22720.701-0.13950.09530.6570.01020.4365-14.8435-37.2150.0659
65.5022-2.5832-1.56924.083-0.28885.19370.0780.5979-0.4334-0.2168-0.2475-0.06320.6502-0.46490.21771.0599-0.06160.02861.0537-0.03490.6082-7.956-48.3838-5.1935
73.948-1.4807-1.14452.25051.03420.47550.1682-0.0252-0.70570.6384-0.62370.4071-0.07210.94770.51590.60340.1369-0.08130.43870.06420.5934-27.0721-45.65929.7084
87.61143.928-6.55762.4523-4.10898.2689-0.65520.4289-0.2137-0.5060.807-0.6230.9787-0.13640.13560.9573-0.0286-0.07850.7831-0.01510.5212-15.0632-65.126712.9139
93.73351.4768-5.47376.97-1.94718.18721.2408-0.49990.21290.93240.26030.7269-0.19590.9645-1.41831.04540.2075-0.0061.09280.18190.6807-13.7576-63.71922.9079
103.81974.7651-4.29428.0792-5.55888.3654-0.52830.220.173-0.2550.23710.40290.2199-0.31610.24740.51520.03710.0660.77010.02530.4638-10.8173-55.531426.0916
113.4785-0.17230.89177.7919-1.49018.42830.02520.2235-0.04220.7588-0.18110.1684-0.05371.63460.10520.54710.0410.10120.8172-0.01890.4522-10.2137-47.830133.0388
126.3782-0.7294-4.16226.5546-1.99844.23960.3244-0.461-0.0881-0.593-0.3866-0.0998-0.55750.53590.37160.8877-0.1577-0.00650.8277-0.07060.3789-15.2576-40.027640.6436
137.6238-2.4462.77512.6933-4.12746.9499-0.0152-0.5407-0.0938-0.05970.2480.077-0.1885-0.3155-0.25170.8815-0.05740.11330.6515-0.15550.408-24.5474-34.584152.4209
144.7628-3.64042.55.6339-5.11278.47231.94850.5824-1.4518-1.3173-0.66820.38411.36090.851-0.64371.0185-0.01450.00050.6611-0.13910.4962-33.4421-40.048354.1986
152.0798-0.511-1.6784.39710.21072.431-0.03-0.53371.06910.2914-0.18990.3522-0.13260.3022-0.14480.8512-0.20470.2521.256-0.0880.584-37.0651-36.043863.8054
165.6022-2.4949-1.95083.77261.08822.9751-0.48020.2558-0.330.1024-0.05280.3278-0.1064-0.93330.45960.9867-0.13270.15250.58130.00780.5798-30.9505-46.663153.9889
178.2198-0.0216-2.88742.17121.64516.03430.25890.8492-0.2576-0.5653-0.33520.22520.2279-0.95670.14310.99520.164-0.05990.66350.0260.5791-61.5635-22.914635.8964
180.92960.57450.94875.24883.1765.7858-0.119-0.4972-0.00350.4727-0.20720.16240.2954-0.03180.28240.4330.1144-0.01540.80640.07260.4364-44.5292-7.765558.5632
199.29221.40597.72194.39840.05786.63-1.8489-3.25821.58061.49770.596-0.2897-1.9716-1.72030.5822.60480.36740.5492.20110.04511.2547-48.4068.468576.4147
202.8011.87613.47482.9074.40376.2939-0.1513-1.16280.9723-0.8499-1.38080.1038-2.1545-3.07231.73081.22270.3352-0.00251.4025-0.31581.0991-56.763-1.473763.8296
218.1044.13233.17069.5863-1.25944.0510.3309-2.78120.18962.7653-0.69220.427-1.906-0.20610.11170.4436-0.04770.11421.2821-0.05180.6899-64.740811.163648.3894
228.3883.92033.22999.03351.91419.03060.24890.37890.0592-0.7325-0.23140.3292-0.1805-1.01540.20030.51220.19130.14380.59850.01040.4693-62.982211.400438.3432
239.41053.31343.56635.92011.93928.96850.2110.59-0.69990.10720.0957-0.0867-0.2277-0.5176-0.20770.6518-0.01120.11830.4662-0.00340.3832-54.73048.632635.6363
242.93121.56732.15943.9807-0.45132.507-0.09590.22050.0596-0.67030.35710.28350.32740.10660.04760.75660.18540.00850.55460.07960.5096-48.02874.706528.4665
251.5793-0.220.38285.76150.416.1174-0.1105-0.0521-0.0827-0.2034-0.14510.14250.69630.15270.26570.61720.03540.13050.73220.04710.4378-45.0023-8.197215.4663
269.67680.7973-2.03581.6055-2.00442.8302-1.54341.8091-1.197-0.47380.8863-0.241.3773-1.6050.27171.2682-0.07950.16051.0516-0.15610.697-53.3798-22.32118.3165
277.070.57081.2627.52852.86071.2294-2.43730.9795-1.5046-2.11310.867-1.71052.5213-1.67161.29962.3671-0.42860.73081.9087-0.22511.4007-54.3767-23.6286-1.7216
286.01881.659-3.53821.4145-0.88551.8935-0.87721.0533-0.4312-0.15380.46740.22382.1964-0.55360.65621.5245-0.09140.22361.04680.1170.8263-60.2106-12.553110.6927
294.48234.01710.13497.5134-3.85794.67020.2467-0.06850.32820.8813-0.05690.6174-0.92970.1902-0.22510.59140.11150.11440.721-0.0450.5884-40.5539-9.3271109.558
305.6063-0.75872.06512.8510.06357.55070.0436-0.00420.0426-0.1953-0.10660.1270.3135-0.03110.08750.4083-0.05210.03040.6217-0.06560.3953-55.9708-20.65290.0614
313.0517-2.7192-2.78817.2477-0.49726.27010.36250.2350.0644-0.6752-0.27290.2856-0.6112-0.4966-0.02150.610.0766-0.10031.3502-0.01570.4854-70.9294-10.656972.9086
321.4528-1.58420.23122.8160.71721.62380.53160.21620.1925-0.35960.00390.1945-1.3572-0.3538-0.39030.9480.0790.08331.09120.18710.7299-62.5954-4.592575.9685
336.0563-4.1698-1.1343.28430.73881.6881-0.21-1.90950.38271.51630.9740.14790.37881.9881-0.40811.29910.00230.06621.63040.02420.9256-21.2236-28.0318101.5227
347.9163-2.8591-0.04499.22261.86231.7973-0.5632-0.6361-0.19311.50860.97360.2121-0.77420.2048-0.43011.13580.27120.21181.26850.04430.6632-17.5266-33.648994.1268
351.32361.41130.58432.37371.89383.71830.1793-0.2378-0.0520.0866-0.0618-0.1235-0.18591.1049-0.12090.48070.16890.02491.108-0.02610.5218-12.6199-50.878679.2701
363.95252.05541.17311.7676-0.0274.4761-0.22090.22520.0833-0.38530.0688-0.1360.00120.21260.08820.68930.23050.04280.6428-0.01030.4984-28.8007-63.551660.0434
375.3611.68832.74834.91382.46243.8058-0.6056-0.8208-0.01651.08370.47250.60861.0982-0.56770.30240.7282-0.17240.05550.88690.02090.589-23.4283-49.763370.3422
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 9 through 39 )A9 - 39
2X-RAY DIFFRACTION2chain 'A' and (resid 40 through 66 )A40 - 66
3X-RAY DIFFRACTION3chain 'A' and (resid 67 through 108 )A67 - 108
4X-RAY DIFFRACTION4chain 'A' and (resid 109 through 234 )A109 - 234
5X-RAY DIFFRACTION5chain 'A' and (resid 235 through 305 )A235 - 305
6X-RAY DIFFRACTION6chain 'A' and (resid 306 through 333 )A306 - 333
7X-RAY DIFFRACTION7chain 'A' and (resid 334 through 351 )A334 - 351
8X-RAY DIFFRACTION8chain 'B' and (resid 11 through 39 )B11 - 39
9X-RAY DIFFRACTION9chain 'B' and (resid 40 through 66 )B40 - 66
10X-RAY DIFFRACTION10chain 'B' and (resid 67 through 108 )B67 - 108
11X-RAY DIFFRACTION11chain 'B' and (resid 109 through 150 )B109 - 150
12X-RAY DIFFRACTION12chain 'B' and (resid 151 through 207 )B151 - 207
13X-RAY DIFFRACTION13chain 'B' and (resid 208 through 276 )B208 - 276
14X-RAY DIFFRACTION14chain 'B' and (resid 277 through 292 )B277 - 292
15X-RAY DIFFRACTION15chain 'B' and (resid 293 through 318 )B293 - 318
16X-RAY DIFFRACTION16chain 'B' and (resid 319 through 351 )B319 - 351
17X-RAY DIFFRACTION17chain 'C' and (resid 8 through 124 )C8 - 124
18X-RAY DIFFRACTION18chain 'C' and (resid 125 through 291 )C125 - 291
19X-RAY DIFFRACTION19chain 'C' and (resid 292 through 318 )C292 - 318
20X-RAY DIFFRACTION20chain 'C' and (resid 319 through 351 )C319 - 351
21X-RAY DIFFRACTION21chain 'D' and (resid 11 through 39 )D11 - 39
22X-RAY DIFFRACTION22chain 'D' and (resid 40 through 66 )D40 - 66
23X-RAY DIFFRACTION23chain 'D' and (resid 67 through 108 )D67 - 108
24X-RAY DIFFRACTION24chain 'D' and (resid 109 through 150 )D109 - 150
25X-RAY DIFFRACTION25chain 'D' and (resid 151 through 263 )D151 - 263
26X-RAY DIFFRACTION26chain 'D' and (resid 264 through 290 )D264 - 290
27X-RAY DIFFRACTION27chain 'D' and (resid 291 through 318 )D291 - 318
28X-RAY DIFFRACTION28chain 'D' and (resid 319 through 351 )D319 - 351
29X-RAY DIFFRACTION29chain 'E' and (resid 11 through 108 )E11 - 108
30X-RAY DIFFRACTION30chain 'E' and (resid 109 through 234 )E109 - 234
31X-RAY DIFFRACTION31chain 'E' and (resid 235 through 318 )E235 - 318
32X-RAY DIFFRACTION32chain 'E' and (resid 319 through 351 )E319 - 351
33X-RAY DIFFRACTION33chain 'F' and (resid 9 through 41 )F9 - 41
34X-RAY DIFFRACTION34chain 'F' and (resid 42 through 82 )F42 - 82
35X-RAY DIFFRACTION35chain 'F' and (resid 83 through 234 )F83 - 234
36X-RAY DIFFRACTION36chain 'F' and (resid 235 through 334 )F235 - 334
37X-RAY DIFFRACTION37chain 'F' and (resid 335 through 351 )F335 - 351

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