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- PDB-6roc: Crystal structure of Borrelia burgdorferi outer surface protein B... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6roc | ||||||
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Title | Crystal structure of Borrelia burgdorferi outer surface protein BBA69, mutant Leu214Met (Se-Met data) | ||||||
![]() | Putative surface protein | ||||||
![]() | MEMBRANE PROTEIN / Outer surface protein / PFam54 | ||||||
Function / homology | Borrelia lipoprotein paralogus family 54/60 / Borrelia Bbcrasp-1 domain containing protein / membrane / Putative surface protein![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Brangulis, K. / Akopjana, I. / Petrovskis, I. / Kazaks, A. / Tars, K. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal structure of Borrelia burgdorferi outer surface protein BBA69 in comparison to the paralogous protein CspA. Authors: Brangulis, K. / Akopjana, I. / Petrovskis, I. / Kazaks, A. / Tars, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 84.7 KB | Display | ![]() |
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PDB format | ![]() | 65.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 293.8 KB | Display | ![]() |
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Full document | ![]() | 297.7 KB | Display | |
Data in XML | ![]() | 15 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 22469.486 Da / Num. of mol.: 2 / Mutation: Leu214 mutated to Met Source method: isolated from a genetically manipulated source Details: First 4 residues (GAMG) are remnants from the expression tag. Source: (gene. exp.) ![]() Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680 / Gene: BB_A69 / Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.25 Å3/Da / Density % sol: 45.25 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, sitting drop / Details: 0.1M (NH4)2SO4 0.1M Tris pH 8.0 25% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | |||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Jan 24, 2019 | |||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength | Wavelength: 0.9797 Å / Relative weight: 1 | |||||||||||||||
Reflection twin |
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Reflection | Resolution: 2.9→52.19 Å / Num. obs: 8521 / % possible obs: 96.7 % / Redundancy: 4.8 % / Rmerge(I) obs: 0.063 / Net I/σ(I): 13.2 | |||||||||||||||
Reflection shell | Resolution: 2.9→3.08 Å / Rmerge(I) obs: 0.225 / Mean I/σ(I) obs: 5.6 / Num. unique obs: 1374 / % possible all: 95.9 |
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Processing
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Refinement | Method to determine structure: ![]() Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 162.17 Å2 / Biso mean: 70.733 Å2 / Biso min: 30 Å2
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Refinement step | Cycle: final / Resolution: 2.9→52.19 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.9→2.975 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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