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Yorodumi- PDB-6prk: X-ray Crystal Structure of Bacillus subtilis RicA in complex with RicF -
+Open data
-Basic information
Entry | Database: PDB / ID: 6prk | |||||||||
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Title | X-ray Crystal Structure of Bacillus subtilis RicA in complex with RicF | |||||||||
Components |
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Keywords | METAL BINDING PROTEIN / RNA processing / biofilms / competence / sporulation | |||||||||
Function / homology | Function and homology information establishment of competence for transformation / sporulation resulting in formation of a cellular spore / cytoplasm Similarity search - Function | |||||||||
Biological species | Bacillus subtilis (bacteria) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.2 Å | |||||||||
Authors | Khaja, F.T. / Jeffrey, P.D. / Neiditch, M.B. / Dubnau, D. | |||||||||
Funding support | United States, 2items
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Citation | Journal: Mbio / Year: 2019 Title: Structure-Function Studies of the Bacillus subtilis Ric Proteins Identify the Fe-S Cluster-Ligating Residues and Their Roles in Development and RNA Processing. Authors: Adusei-Danso, F. / Khaja, F.T. / DeSantis, M. / Jeffrey, P.D. / Dubnau, E. / Demeler, B. / Neiditch, M.B. / Dubnau, D. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6prk.cif.gz | 111.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6prk.ent.gz | 87.2 KB | Display | PDB format |
PDBx/mmJSON format | 6prk.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pr/6prk ftp://data.pdbj.org/pub/pdb/validation_reports/pr/6prk | HTTPS FTP |
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-Related structure data
Related structure data | 6prhSC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 14399.207 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus subtilis (strain 168) (bacteria) Strain: 168 / Gene: ylbF, BSU14990 / Production host: Escherichia coli (E. coli) / References: UniProt: O34412 |
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#2: Protein | Mass: 14180.994 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus subtilis (strain 168) (bacteria) Strain: 168 / Gene: ymcA, BSU17020 / Production host: Escherichia coli (E. coli) / References: UniProt: O31779 |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.44 Å3/Da / Density % sol: 64.25 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 18% (vol/vol) PEG500 MME, 8% (vol/vol) MPD, 100 mM sodium nitrate, 100 mM MOPS, Sodium HEPES pH 7.3 |
-Data collection
Diffraction | Mean temperature: 93 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL9-2 / Wavelength: 0.88557 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Dec 15, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.88557 Å / Relative weight: 1 |
Reflection | Resolution: 3.2→50 Å / Num. obs: 6195 / % possible obs: 99.9 % / Redundancy: 9.95 % / Biso Wilson estimate: 105.49 Å2 / CC1/2: 0.99 / Rrim(I) all: 0.085 / Rsym value: 0.08 / Net I/σ(I): 17.24 |
Reflection shell | Resolution: 3.2→3.26 Å / Num. unique obs: 312 / CC1/2: 0.81 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 6PRH Resolution: 3.2→43.04 Å / Cor.coef. Fo:Fc: 0.961 / Cor.coef. Fo:Fc free: 0.944 / Cross valid method: THROUGHOUT / σ(F): 0 / SU Rfree Blow DPI: 0.475
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Displacement parameters | Biso max: 232.24 Å2 / Biso mean: 144.79 Å2 / Biso min: 89.92 Å2
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Refine analyze | Luzzati coordinate error obs: 0.41 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 3.2→43.04 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.2→3.58 Å / Rfactor Rfree error: 0 / Total num. of bins used: 5
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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