- PDB-6dlk: Crystal structure of DNA polymerase III subunit beta from Rickett... -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 6dlk
Title
Crystal structure of DNA polymerase III subunit beta from Rickettsia rickettsii
Components
Beta sliding clamp
Keywords
TRANSFERASE / SSGCID / DnaN / DNA polymerase III beta / potential drug target / sliding clamp / Structural Genomics / Seattle Structural Genomics Center for Infectious Disease
Function / homology
Function and homology information
DNA polymerase III complex / 3'-5' exonuclease activity / DNA replication / DNA-directed DNA polymerase activity / DNA binding / cytoplasm Similarity search - Function
DNA polymerase III, beta sliding clamp / DNA polymerase III, beta sliding clamp, N-terminal / DNA polymerase III, beta sliding clamp, C-terminal / DNA polymerase III, beta sliding clamp, central / DNA polymerase III beta subunit, N-terminal domain / DNA polymerase III beta subunit, central domain / DNA polymerase III beta subunit, C-terminal domain / DNA polymerase III beta subunit Similarity search - Domain/homology
Mass: 18.015 Da / Num. of mol.: 537 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.66 Å3/Da / Density % sol: 53.74 %
Crystal grow
Temperature: 290 K / Method: vapor diffusion, sitting drop / pH: 5.6 Details: Rigaku Reagents Top96 G6: (0.5 M Ammonium Sulfate, 0.1 M Sodium Citrate:Citric Acid pH 5.6, 1 M Lithium Sulfate) mixed 1:1 with 20 mg/mL protein in sitting drop vapor diffusion. Tray id ...Details: Rigaku Reagents Top96 G6: (0.5 M Ammonium Sulfate, 0.1 M Sodium Citrate:Citric Acid pH 5.6, 1 M Lithium Sulfate) mixed 1:1 with 20 mg/mL protein in sitting drop vapor diffusion. Tray id 291006g9, crystal id qtv9-3
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Data collection
Diffraction
Mean temperature: 100 K
Diffraction source
Source: SYNCHROTRON / Site: ALS / Beamline: 8.2.1 / Wavelength: 0.97872 Å
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