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- PDB-6oik: Muscarinic acetylcholine receptor 2-Go complex -

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Entry
Database: PDB / ID: 6oik
TitleMuscarinic acetylcholine receptor 2-Go complex
Components
  • (Guanine nucleotide-binding protein ...) x 3
  • Antibody fragment
  • Muscarinic acetylcholine receptor M2,Muscarinic acetylcholine receptor M2
KeywordsSIGNALING PROTEIN / G-protein coupled receptor-G-protein complex / neurotransmitter receptor
Function / homology
Function and homology information


ADP signalling through P2Y purinoceptor 12 / Adrenaline,noradrenaline inhibits insulin secretion / Ca2+ pathway / G alpha (q) signalling events / G alpha (12/13) signalling events / G beta:gamma signalling through PI3Kgamma / G beta:gamma signalling through PLC beta / G alpha (s) signalling events / Prostacyclin signalling through prostacyclin receptor / ADP signalling through P2Y purinoceptor 1 ...ADP signalling through P2Y purinoceptor 12 / Adrenaline,noradrenaline inhibits insulin secretion / Ca2+ pathway / G alpha (q) signalling events / G alpha (12/13) signalling events / G beta:gamma signalling through PI3Kgamma / G beta:gamma signalling through PLC beta / G alpha (s) signalling events / Prostacyclin signalling through prostacyclin receptor / ADP signalling through P2Y purinoceptor 1 / G alpha (i) signalling events / Inactivation, recovery and regulation of the phototransduction cascade / Glucagon signaling in metabolic regulation / G-protein activation / Activation of the phototransduction cascade / Non-genomic estrogen signaling / G alpha (z) signalling events / Presynaptic function of Kainate receptors / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / Activation of G protein gated Potassium channels / Cargo recognition for clathrin-mediated endocytosis / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / Olfactory Signaling Pathway / Clathrin-mediated endocytosis / G beta:gamma signalling through BTK / G beta:gamma signalling through CDC42 / Thrombin signalling through proteinase activated receptors (PARs) / Muscarinic acetylcholine receptors / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / Vasopressin regulates renal water homeostasis via Aquaporins / Glucagon-type ligand receptors / PLC beta mediated events / Thromboxane signalling through TP receptor / phospholipase C-activating G protein-coupled acetylcholine receptor signaling pathway / G protein-coupled acetylcholine receptor activity / symmetric synapse / adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway / corticotropin-releasing hormone receptor 1 binding / cholinergic synapse / dopamine receptor signaling pathway / regulation of smooth muscle contraction / G protein-coupled serotonin receptor activity / neurotransmitter receptor activity / mu-type opioid receptor binding / G protein-coupled serotonin receptor binding / sensory perception of taste / cellular response to catecholamine stimulus / cardiac muscle cell apoptotic process / G protein-coupled acetylcholine receptor signaling pathway / protein heterotrimerization / alkylglycerophosphoethanolamine phosphodiesterase activity / arrestin family protein binding / G-protein beta-subunit binding / rhodopsin mediated signaling pathway / adenylate cyclase-activating dopamine receptor signaling pathway / photoreceptor outer segment membrane / G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger / photoreceptor disc membrane / G-protein beta/gamma-subunit complex binding / spectrin binding / negative regulation of calcium ion transport / heterotrimeric G-protein complex / Wnt signaling pathway, calcium modulating pathway / GTPase activating protein binding / axon terminus / G protein-coupled receptor activity / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / extracellular vesicle / asymmetric synapse / response to morphine / cellular response to prostaglandin E stimulus / regulation of heart contraction / retina development in camera-type eye / G protein-coupled receptor binding / phospholipase C-activating G protein-coupled receptor signaling pathway / integral component of postsynaptic membrane / clathrin-coated vesicle membrane / GTPase binding / photoreceptor inner segment / forebrain development / muscle contraction / locomotory behavior / regulation of synaptic vesicle exocytosis / response to cytokine / response to virus / integral component of presynaptic membrane / positive regulation of GTPase activity / cell body / neuron projection development / odontogenesis of dentin-containing tooth / nervous system development / lysosomal membrane / response to hydrogen peroxide / protein folding / Ras protein signal transduction / chemical synaptic transmission / cellular response to hypoxia / membrane organization / positive regulation of cytosolic calcium ion concentration / platelet activation
G-protein coupled receptors family 1 signature. / G-protein alpha subunit / GGL domain / WD domain, G-beta repeat / Trp-Asp (WD) repeats signature. / G-protein gamma subunit domain profile. / Trp-Asp (WD) repeats profile. / G-protein gamma-like domain superfamily / G-protein coupled receptors family 1 profile. / Trp-Asp (WD) repeats circular profile. ...G-protein coupled receptors family 1 signature. / G-protein alpha subunit / GGL domain / WD domain, G-beta repeat / Trp-Asp (WD) repeats signature. / G-protein gamma subunit domain profile. / Trp-Asp (WD) repeats profile. / G-protein gamma-like domain superfamily / G-protein coupled receptors family 1 profile. / Trp-Asp (WD) repeats circular profile. / 7 transmembrane receptor (rhodopsin family) / G protein alpha subunit, helical insertion / G-protein beta WD-40 repeat / WD40 repeat, conserved site / WD40-repeat-containing domain / GPCR, rhodopsin-like, 7TM / Guanine nucleotide-binding protein, beta subunit / WD40/YVTN repeat-like-containing domain superfamily / G-protein gamma-like domain / G-protein, gamma subunit / WD40-repeat-containing domain superfamily / WD40 repeat / G-protein, beta subunit / G-protein alpha subunit, group I / Muscarinic acetylcholine receptor M2 / Guanine nucleotide binding protein (G-protein), alpha subunit / Muscarinic acetylcholine receptor family / G protein-coupled receptor, rhodopsin-like / G-alpha domain profile. / P-loop containing nucleoside triphosphate hydrolase
Muscarinic acetylcholine receptor M2 / Guanine nucleotide-binding protein G(o) subunit alpha / Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 / Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
Specimen sourceHomo sapiens (human)
Mus musculus (house mouse)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsMaeda, S. / Qianhui, Q. / Skiniotis, G. / Kobilka, B.
Funding supportUnited States , 1件
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical SciencesR01GM083118United States
CitationJournal: Science / Year: 2019
Title: Structures of the M1 and M2 muscarinic acetylcholine receptor/G-protein complexes.
Authors: Shoji Maeda / Qianhui Qu / Michael J Robertson / Georgios Skiniotis / Brian K Kobilka /
Abstract: Muscarinic acetylcholine receptors are G protein-coupled receptors that respond to acetylcholine and play important signaling roles in the nervous system. There are five muscarinic receptor subtypes ...Muscarinic acetylcholine receptors are G protein-coupled receptors that respond to acetylcholine and play important signaling roles in the nervous system. There are five muscarinic receptor subtypes (M1R to M5R), which, despite sharing a high degree of sequence identity in the transmembrane region, couple to different heterotrimeric GTP-binding proteins (G proteins) to transmit signals. M1R, M3R, and M5R couple to the G family, whereas M2R and M4R couple to the G family. Here, we present and compare the cryo-electron microscopy structures of M1R in complex with G and M2R in complex with G The M1R-G complex exhibits distinct features, including an extended transmembrane helix 5 and carboxyl-terminal receptor tail that interacts with G protein. Detailed analysis of these structures provides a framework for understanding the molecular determinants of G-protein coupling selectivity.
Validation Report
SummaryFull reportAbout validation report
DateDeposition: Apr 9, 2019 / Release: May 8, 2019
RevisionDateData content typeGroupCategoryItemProviderType
1.0May 8, 2019Structure modelrepositoryInitial release
1.1May 22, 2019Structure modelData collection / Database referencescitation / citation_author_citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.name

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Structure visualization

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Assembly

Deposited unit
R: Muscarinic acetylcholine receptor M2,Muscarinic acetylcholine receptor M2
A: Guanine nucleotide-binding protein G(o) subunit alpha
B: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
G: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
H: Antibody fragment
hetero molecules


Theoretical massNumber of molelcules
Total (without water)153,3997
Polymers152,7645
Non-polymers6352
Water0
1


TypeNameSymmetry operationNumber
identity operation1_5551
Buried area9700 Å2
ΔGint-67 kcal/mol
Surface area47980 Å2

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Components

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Protein/peptide , 2 types, 2 molecules RH

#1: Protein/peptide Muscarinic acetylcholine receptor M2,Muscarinic acetylcholine receptor M2


Mass: 39729.910 Da / Num. of mol.: 1 / Mutation: N8A, N11D, N14D,N11D, N14D
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: CHRM2 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P08172
#5: Protein/peptide Antibody fragment /


Mass: 27340.482 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Production host: Trichoplusia ni (cabbage looper)

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Guanine nucleotide-binding protein ... , 3 types, 3 molecules ABG

#2: Protein/peptide Guanine nucleotide-binding protein G(o) subunit alpha


Mass: 40104.551 Da / Num. of mol.: 1 / Mutation: E9D, R10K, L13V, A18M
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: GNAO1 / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: P09471
#3: Protein/peptide Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 / Transducin beta chain 1


Mass: 37728.152 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: GNB1 / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: P62873
#4: Protein/peptide Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 / G gamma-I


Mass: 7861.143 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: GNG2 / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: P59768

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Non-polymers , 2 types, 2 molecules

#6: Chemical ChemComp-IXO / 4-(4,5-dihydro-1,2-oxazol-3-yloxy)-N,N,N-trimethylbut-2-yn-1-aminium / Iperoxo


Mass: 197.254 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C10H17N2O2
#7: Chemical ChemComp-2CU / 3-amino-5-chloro-N-cyclopropyl-4-methyl-6-[2-(4-methylpiperazin-1-yl)-2-oxoethoxy]thieno[2,3-b]pyridine-2-carboxamide / LY2119620 positive allosteric modulator of M2/M4 receptor


Mass: 437.944 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C19H24ClN5O3S

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

Component

Source: RECOMBINANT / Type: COMPLEX

IDNameEntity IDParent-ID
1muscarinic acetylcholine receptor 2 in complex with heterotrimeric GoA protein1, 2, 3, 4, 50
2Muscarinic acetylcholine receptor M211
3Guanine nucleotide-binding protein G(o) subunit alpha, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, Antibody fragment2, 3, 4, 51
Molecular weightValue: 0.15 MDa / Experimental value: NO
Source (natural)

Ncbi tax-ID: 9606 / Organism: Homo sapiens (human)

IDEntity assembly-ID
12
23
Source (recombinant)
IDEntity assembly-IDOrganismNcbi tax-ID
12Spodoptera frugiperda (fall armyworm)7108
23Trichoplusia ni (cabbage looper)7111
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportDetails: unspecified
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy
Image recordingElectron dose: 7 e/Å2 / Detector mode: COUNTING / Film or detector model: GATAN K2 SUMMIT (4k x 4k)

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Processing

EM softwareName: RELION / Version: 2.1 / Category: image acquisition
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 261730 / Symmetry type: POINT

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