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- EMDB-20079: Muscarinic acetylcholine receptor 2-Go complex -

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Basic information

Entry
Database: EMDB / ID: EMD-20079
TitleMuscarinic acetylcholine receptor 2-Go complex
Map data
Samplemuscarinic acetylcholine receptor 2 in complex with heterotrimeric GoA protein
  • (Muscarinic acetylcholine receptor ...) x 2
  • (Guanine nucleotide-binding protein ...) x 4
  • Antibody fragment
  • (ligand) x 2
Function / homology
Function and homology information


ADP signalling through P2Y purinoceptor 12 / Adrenaline,noradrenaline inhibits insulin secretion / Ca2+ pathway / G alpha (q) signalling events / G alpha (12/13) signalling events / G beta:gamma signalling through PI3Kgamma / G beta:gamma signalling through PLC beta / G alpha (s) signalling events / Prostacyclin signalling through prostacyclin receptor / ADP signalling through P2Y purinoceptor 1 ...ADP signalling through P2Y purinoceptor 12 / Adrenaline,noradrenaline inhibits insulin secretion / Ca2+ pathway / G alpha (q) signalling events / G alpha (12/13) signalling events / G beta:gamma signalling through PI3Kgamma / G beta:gamma signalling through PLC beta / G alpha (s) signalling events / Prostacyclin signalling through prostacyclin receptor / ADP signalling through P2Y purinoceptor 1 / G alpha (i) signalling events / Inactivation, recovery and regulation of the phototransduction cascade / Glucagon signaling in metabolic regulation / G-protein activation / Activation of the phototransduction cascade / Non-genomic estrogen signaling / G alpha (z) signalling events / Presynaptic function of Kainate receptors / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / Activation of G protein gated Potassium channels / Cargo recognition for clathrin-mediated endocytosis / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / Olfactory Signaling Pathway / Clathrin-mediated endocytosis / G beta:gamma signalling through BTK / G beta:gamma signalling through CDC42 / Thrombin signalling through proteinase activated receptors (PARs) / Muscarinic acetylcholine receptors / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / Vasopressin regulates renal water homeostasis via Aquaporins / Glucagon-type ligand receptors / PLC beta mediated events / Thromboxane signalling through TP receptor / phospholipase C-activating G protein-coupled acetylcholine receptor signaling pathway / G protein-coupled acetylcholine receptor activity / symmetric synapse / adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway / corticotropin-releasing hormone receptor 1 binding / cholinergic synapse / dopamine receptor signaling pathway / regulation of smooth muscle contraction / G protein-coupled serotonin receptor activity / neurotransmitter receptor activity / mu-type opioid receptor binding / G protein-coupled serotonin receptor binding / sensory perception of taste / cellular response to catecholamine stimulus / cardiac muscle cell apoptotic process / G protein-coupled acetylcholine receptor signaling pathway / protein heterotrimerization / alkylglycerophosphoethanolamine phosphodiesterase activity / arrestin family protein binding / G-protein beta-subunit binding / rhodopsin mediated signaling pathway / adenylate cyclase-activating dopamine receptor signaling pathway / photoreceptor outer segment membrane / G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger / photoreceptor disc membrane / G-protein beta/gamma-subunit complex binding / spectrin binding / negative regulation of calcium ion transport / heterotrimeric G-protein complex / Wnt signaling pathway, calcium modulating pathway / GTPase activating protein binding / axon terminus / G protein-coupled receptor activity / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / extracellular vesicle / asymmetric synapse / response to morphine / cellular response to prostaglandin E stimulus / regulation of heart contraction / retina development in camera-type eye / G protein-coupled receptor binding / phospholipase C-activating G protein-coupled receptor signaling pathway / integral component of postsynaptic membrane / clathrin-coated vesicle membrane / GTPase binding / photoreceptor inner segment / forebrain development / muscle contraction / locomotory behavior / regulation of synaptic vesicle exocytosis / response to cytokine / response to virus / integral component of presynaptic membrane / positive regulation of GTPase activity / cell body / neuron projection development / odontogenesis of dentin-containing tooth / nervous system development / lysosomal membrane / response to hydrogen peroxide / protein folding / Ras protein signal transduction / chemical synaptic transmission / cellular response to hypoxia / membrane organization / positive regulation of cytosolic calcium ion concentration / platelet activation
G-protein coupled receptors family 1 signature. / G-protein alpha subunit / GGL domain / WD domain, G-beta repeat / Trp-Asp (WD) repeats signature. / G-protein gamma subunit domain profile. / Trp-Asp (WD) repeats profile. / G-protein gamma-like domain superfamily / G-protein coupled receptors family 1 profile. / Trp-Asp (WD) repeats circular profile. ...G-protein coupled receptors family 1 signature. / G-protein alpha subunit / GGL domain / WD domain, G-beta repeat / Trp-Asp (WD) repeats signature. / G-protein gamma subunit domain profile. / Trp-Asp (WD) repeats profile. / G-protein gamma-like domain superfamily / G-protein coupled receptors family 1 profile. / Trp-Asp (WD) repeats circular profile. / 7 transmembrane receptor (rhodopsin family) / G protein alpha subunit, helical insertion / G-protein beta WD-40 repeat / WD40 repeat, conserved site / WD40-repeat-containing domain / GPCR, rhodopsin-like, 7TM / Guanine nucleotide-binding protein, beta subunit / WD40/YVTN repeat-like-containing domain superfamily / G-protein gamma-like domain / G-protein, gamma subunit / WD40-repeat-containing domain superfamily / WD40 repeat / G-protein, beta subunit / G-protein alpha subunit, group I / Muscarinic acetylcholine receptor M2 / Guanine nucleotide binding protein (G-protein), alpha subunit / Muscarinic acetylcholine receptor family / G protein-coupled receptor, rhodopsin-like / G-alpha domain profile. / P-loop containing nucleoside triphosphate hydrolase
Muscarinic acetylcholine receptor M2 / Guanine nucleotide-binding protein G(o) subunit alpha / Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 / Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
SourceHomo sapiens (human) / Mus musculus (house mouse)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.6 Å
AuthorsMaeda S / Qianhui Q / Skiniotis G / Kobilka B
CitationJournal: Science / Year: 2019
Title: Structures of the M1 and M2 muscarinic acetylcholine receptor/G-protein complexes.
Authors: Shoji Maeda / Qianhui Qu / Michael J Robertson / Georgios Skiniotis / Brian K Kobilka /
Abstract: Muscarinic acetylcholine receptors are G protein-coupled receptors that respond to acetylcholine and play important signaling roles in the nervous system. There are five muscarinic receptor subtypes ...Muscarinic acetylcholine receptors are G protein-coupled receptors that respond to acetylcholine and play important signaling roles in the nervous system. There are five muscarinic receptor subtypes (M1R to M5R), which, despite sharing a high degree of sequence identity in the transmembrane region, couple to different heterotrimeric GTP-binding proteins (G proteins) to transmit signals. M1R, M3R, and M5R couple to the G family, whereas M2R and M4R couple to the G family. Here, we present and compare the cryo-electron microscopy structures of M1R in complex with G and M2R in complex with G The M1R-G complex exhibits distinct features, including an extended transmembrane helix 5 and carboxyl-terminal receptor tail that interacts with G protein. Detailed analysis of these structures provides a framework for understanding the molecular determinants of G-protein coupling selectivity.
Validation ReportPDB-ID: 6oik

SummaryFull reportAbout validation report
DateDeposition: Apr 9, 2019 / Header (metadata) release: May 1, 2019 / Map release: May 8, 2019 / Update: May 22, 2019

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.025
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.025
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: : PDB-6oik
  • Surface level: 0.025
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

FileDownload / File: emd_20079.map.gz / Format: CCP4 / Size: 40.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.06 Å/pix.
x 220 pix.
= 233.2 Å
1.06 Å/pix.
x 220 pix.
= 233.2 Å
1.06 Å/pix.
x 220 pix.
= 233.2 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.06 Å
Density
Contour LevelBy AUTHOR: 0.01 / Movie #1: 0.025
Minimum - Maximum-0.072588935 - 0.12493647
Average (Standard dev.)0.000032668606 (±0.0033500856)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions220220220
Spacing220220220
CellA=B=C: 233.19998 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.061.061.06
M x/y/z220220220
origin x/y/z0.0000.0000.000
length x/y/z233.200233.200233.200
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS220220220
D min/max/mean-0.0730.1250.000

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Supplemental data

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Sample components

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Entire muscarinic acetylcholine receptor 2 in complex with heterotrimeri...

EntireName: muscarinic acetylcholine receptor 2 in complex with heterotrimeric GoA protein
Number of components: 10

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Component #1: protein, muscarinic acetylcholine receptor 2 in complex with hete...

ProteinName: muscarinic acetylcholine receptor 2 in complex with heterotrimeric GoA protein
Recombinant expression: No
MassTheoretical: 150 kDa

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Component #2: protein, Muscarinic acetylcholine receptor M2

ProteinName: Muscarinic acetylcholine receptor M2 / Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Spodoptera frugiperda (fall armyworm)

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Component #3: protein, Guanine nucleotide-binding protein G(o) subunit alpha, G...

ProteinName: Guanine nucleotide-binding protein G(o) subunit alpha, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, Antibody fragment
Recombinant expression: No
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Trichoplusia ni (cabbage looper)

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Component #4: protein, Muscarinic acetylcholine receptor M2,Muscarinic acetylch...

ProteinName: Muscarinic acetylcholine receptor M2,Muscarinic acetylcholine receptor M2
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 39.72991 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Spodoptera frugiperda (fall armyworm)

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Component #5: protein, Guanine nucleotide-binding protein G(o) subunit alpha

ProteinName: Guanine nucleotide-binding protein G(o) subunit alpha / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 40.104551 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Trichoplusia ni (cabbage looper)

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Component #6: protein, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subuni...

ProteinName: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 37.728152 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Trichoplusia ni (cabbage looper)

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Component #7: protein, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subuni...

ProteinName: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 7.861143 kDa
SourceSpecies: Homo sapiens (human)
Source (engineered)Expression System: Trichoplusia ni (cabbage looper)

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Component #8: protein, Antibody fragment

ProteinName: Antibody fragment / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 27.340482 kDa
SourceSpecies: Mus musculus (house mouse)
Source (engineered)Expression System: Trichoplusia ni (cabbage looper)

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Component #9: ligand, 4-(4,5-dihydro-1,2-oxazol-3-yloxy)-N,N,N-trimethylbut-2-y...

LigandName: 4-(4,5-dihydro-1,2-oxazol-3-yloxy)-N,N,N-trimethylbut-2-yn-1-aminium
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.197254 kDa

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Component #10: ligand, 3-amino-5-chloro-N-cyclopropyl-4-methyl-6-[2-(4-methylpip...

LigandName: 3-amino-5-chloro-N-cyclopropyl-4-methyl-6-[2-(4-methylpiperazin-1-yl)-2-oxoethoxy]thieno[2,3-b]pyridine-2-carboxamide
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 0.437944 kDa

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Experimental details

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Sample preparation

SpecimenSpecimen state: Particle / Method: cryo EM
Sample solutionpH: 7.5
Support filmunspecified
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 7 e/Å2 / Illumination mode: FLOOD BEAM
LensImaging mode: BRIGHT FIELD
Specimen HolderModel: OTHER
CameraDetector: GATAN K2 SUMMIT (4k x 4k)

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Image processing

ProcessingMethod: single particle reconstruction / Number of projections: 261730
3D reconstructionResolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF

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Atomic model buiding

Output model

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