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- PDB-6oat: Structure of the Ganjam virus OTU bound to sheep ISG15 -

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Basic information

Entry
Database: PDB / ID: 6oat
TitleStructure of the Ganjam virus OTU bound to sheep ISG15
Components
  • Interferon stimulated gene 17
  • RNA-dependent RNA polymerase
KeywordsHYDROLASE / DUB / deISGylase / Innate Immunity
Function / homology
Function and homology information


viral RNA genome replication / RNA-directed RNA polymerase activity / DNA-templated transcription
Similarity search - Function
: / Cathepsin B; Chain A - #80 / OTU-like cysteine protease / RNA-directed RNA polymerase L, N-terminal / L protein N-terminus / OTU domain / OTU domain profile. / RNA-dependent RNA polymerase, bunyaviral / Bunyavirus RNA dependent RNA polymerase / RNA-directed RNA polymerase, negative-strand RNA virus ...: / Cathepsin B; Chain A - #80 / OTU-like cysteine protease / RNA-directed RNA polymerase L, N-terminal / L protein N-terminus / OTU domain / OTU domain profile. / RNA-dependent RNA polymerase, bunyaviral / Bunyavirus RNA dependent RNA polymerase / RNA-directed RNA polymerase, negative-strand RNA virus / RdRp of negative ssRNA viruses with segmented genomes catalytic domain profile. / Cathepsin B; Chain A / Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1 / Ubiquitin-like (UB roll) / Ubiquitin family / Ubiquitin homologues / Ubiquitin domain profile. / Ubiquitin-like domain / Ubiquitin-like domain superfamily / Roll / Alpha-Beta Complex / Alpha Beta
Similarity search - Domain/homology
prop-2-en-1-amine / RNA-dependent RNA polymerase / Interferon stimulated gene 17
Similarity search - Component
Biological speciesGanjam virus
Ovis aries (sheep)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.17 Å
AuthorsDzimianski, J.V. / Williams, I.L. / Pegan, S.D.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)1R01AI109008 United States
CitationJournal: Plos One / Year: 2019
Title: Determining the molecular drivers of species-specific interferon-stimulated gene product 15 interactions with nairovirus ovarian tumor domain proteases.
Authors: Dzimianski, J.V. / Scholte, F.E.M. / Williams, I.L. / Langley, C. / Freitas, B.T. / Spengler, J.R. / Bergeron, E. / Pegan, S.D.
History
DepositionMar 18, 2019Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 8, 2020Provider: repository / Type: Initial release
Revision 1.1Oct 11, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_conn
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id
Revision 1.2Dec 25, 2024Group: Advisory / Derived calculations / Structure summary
Category: pdbx_entry_details / pdbx_modification_feature ...pdbx_entry_details / pdbx_modification_feature / pdbx_validate_close_contact / struct_conn

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: RNA-dependent RNA polymerase
B: Interferon stimulated gene 17
C: RNA-dependent RNA polymerase
D: Interferon stimulated gene 17
hetero molecules


Theoretical massNumber of molelcules
Total (without water)75,1146
Polymers74,9994
Non-polymers1142
Water00
1
A: RNA-dependent RNA polymerase
B: Interferon stimulated gene 17
hetero molecules


Theoretical massNumber of molelcules
Total (without water)37,5573
Polymers37,5002
Non-polymers571
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
C: RNA-dependent RNA polymerase
D: Interferon stimulated gene 17
hetero molecules


Theoretical massNumber of molelcules
Total (without water)37,5573
Polymers37,5002
Non-polymers571
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)55.388, 55.388, 497.310
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number169
Space group name H-MP61
Space group name HallP61

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Components

#1: Protein RNA-dependent RNA polymerase


Mass: 20052.535 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Ganjam virus / Production host: Escherichia coli (E. coli) / References: UniProt: A0A191KWA2
#2: Protein Interferon stimulated gene 17


Mass: 17447.154 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Ovis aries (sheep) / Gene: ISG17 / Production host: Escherichia coli (E. coli) / References: UniProt: Q9GKP4
#3: Chemical ChemComp-AYE / prop-2-en-1-amine / ALLYLAMINE


Mass: 57.094 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C3H7N
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.09 Å3/Da / Density % sol: 60.24 %
Crystal growTemperature: 295 K / Method: vapor diffusion, hanging drop / Details: 0.1 M MES pH 5.5, 15% PEG 20,000

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 18-ID / Wavelength: 1 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Dec 9, 2016
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 3.15→50 Å / Num. obs: 12288 / % possible obs: 85 % / Redundancy: 6.7 % / Biso Wilson estimate: 97.7 Å2 / CC1/2: 0.994 / Rpim(I) all: 0.074 / Net I/σ(I): 11.2
Reflection shellResolution: 3.15→3.2 Å / Mean I/σ(I) obs: 1.1 / Num. unique obs: 1139 / CC1/2: 0.662

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Processing

Software
NameVersionClassification
PHENIX1.16_3549refinement
PHENIX1.16_3549refinement
HKL-2000data reduction
HKL-2000data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6OAR

6oar


Resolution: 3.17→41.44 Å / SU ML: 0.5909 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 37.1458
RfactorNum. reflection% reflection
Rfree0.3042 1237 10.11 %
Rwork0.2506 --
obs0.2562 12240 83.82 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso mean: 117.52 Å2
Refinement stepCycle: LAST / Resolution: 3.17→41.44 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4912 0 8 0 4920
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00275016
X-RAY DIFFRACTIONf_angle_d0.616780
X-RAY DIFFRACTIONf_chiral_restr0.0432756
X-RAY DIFFRACTIONf_plane_restr0.0042884
X-RAY DIFFRACTIONf_dihedral_angle_d12.06873000
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.17-3.290.37561280.36721144X-RAY DIFFRACTION77.66
3.29-3.440.38751460.36291253X-RAY DIFFRACTION85.3
3.44-3.620.39831480.3431289X-RAY DIFFRACTION90.49
3.62-3.850.36971350.3081266X-RAY DIFFRACTION85.58
3.85-4.150.31661400.28521253X-RAY DIFFRACTION87.23
4.15-4.570.3391300.2571199X-RAY DIFFRACTION80.84
4.57-5.230.28631380.23611236X-RAY DIFFRACTION85.71
5.23-6.580.32261380.25411210X-RAY DIFFRACTION83.52
6.58-41.440.22991340.16871154X-RAY DIFFRACTION78.58
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
16.087921299613.31214857161-4.53421551014.6646625048-6.215334455028.292957830760.5589497447350.2511606852260.6807175407981.731751198480.197641033512-0.712426937-1.61529271330.622306406992-0.7839240723081.127117824980.173316281716-0.1140369719441.52274112189-0.6879185250931.9469580699815.270731971216.0695370904-3.59099535353
29.462849087060.646343786812-2.682025146976.194602388854.324594380074.058159006370.8085240331390.594592139965-0.2163326785820.108877482562-0.881357827862-2.2342760586-0.576701347015-1.894201067040.1580268892211.347987121920.622088529595-0.1568399238292.113339606120.5480118105791.2635122487510.38204025496.02986042509-8.62200053989
36.4430307825-6.38845778120.5120000039387.65846689952-2.599836021478.7121960588-0.398074679412-0.1199967116191.86687981088-0.679616863015-0.358531848089-2.327954108560.5761030198550.0895272974280.7739581957351.448678177310.516882007055-0.3783853427410.921637437491-0.345733823891.333692165853.203829967194.71745854004-13.8580962985
40.0996107595541-0.0809491697193-0.06978837453180.2944887890160.1865464710820.111554009840.2361086331890.5112499139130.15743598831-0.55787133229-0.364887953179-0.1693036447380.1737202241890.1000103500470.2651329627840.4235402311321.16188601367-0.1729698200991.77329642543-0.1496511979141.320953708048.578708655256.960945346480.335516984225
51.540226866552.804689402090.3551366016699.30344241929-3.8872984265.10901674305-0.647002761628-0.257546430177-0.6187474761550.9573683368971.10050959581-1.6855089837-0.255746958403-0.747339156259-0.3066583918991.11578028749-0.187191160293-0.3030555636131.104324700380.1799356703060.8938903192796.6396407979618.58587770855.97542526039
67.760319288178.385890046930.09684619802329.87349836441.126452530659.068371332750.352454349956-0.5614399137850.632094130832-0.533256460574-0.670784573547-0.75322291041-0.8173923404130.01632458041730.5876175211950.7267761130470.218348323916-0.07886650085920.6319419651750.02328263948850.961798269502-10.307768244919.90969116513.78969443414
74.9075178556-2.28657950457-3.589919221434.804682983190.8069204653982.84177604820.5556637192510.7918348677810.884368615036-0.862367167328-0.317076351429-0.736742649696-0.306091073114-0.852550087543-0.1573473902361.575016420250.501954839129-0.1750327448660.8195628174480.004170522597220.759281484589-10.455315386818.3718719727-3.80339291677
84.127151742720.0157793885514-2.824317513384.97226145364-3.441147660334.307874963511.032706243150.607315790875-0.275168122404-0.3302980324831.421497964840.359825743289-0.4967512784650.0259824774122-1.52366002391.38123107117-0.293679227645-0.4795010720960.7152974921110.2210245087831.30961310207-4.9734411144426.5213517127-6.79959834742
93.256970419830.4364781157971.490349539332.092679341643.279650722955.63163199786-0.09641135365540.5448320016320.356731266488-0.6795041790890.2976962997270.790368304134-2.95071652605-0.270459063551-0.01099710417911.6999882340.173569425876-0.2969824781390.9903303478770.003467501336981.07440275049-13.548812407926.4413160971-5.40985926829
102.9599110204-0.4196918309831.292790317280.743040134203-1.240478999522.185657569180.6404578371590.4711158453610.292592796227-0.7576858035150.5523786295180.471634785250.87005893313-0.0257567834840.6595826283372.283429574721.00054018786-0.5835782325840.739793021350.09760763038930.887473421667-13.839876253717.0156697608-17.5400984328
112.794174094631.610943206792.397513221118.44715749387-0.7180563294753.06219772558-0.4162281771970.2450589850880.0394535705818-0.5657952290611.79249589607-0.5614145712850.913783813225-0.114522775901-1.308095200271.249072176610.08868949184280.2265936031660.6473677885280.01906303426021.18098104431-10.19081709388.76508245711-21.5559055951
127.09713306361-1.961714789215.721509092749.495353711071.713539406188.505943072940.0558228131596-0.3668473724630.1882780971862.400842397860.712377059496-2.209435931011.64438356726-0.491273037251-0.7901722709751.398539916950.242989038248-0.256494798880.746807933371-0.07942051871591.08512740116-10.50594912981.48090145294-16.1536724904
130.041190937664-0.4716232585720.01473137287384.66055621148-0.05386585522511.13311100750.2791354735160.205028334209-1.003871782390.01725403599450.4350121907012.467632911740.326721201605-0.105835253743-0.301888343812.574702836591.02914045482-0.8354567623050.833520101198-0.2769139245691.46183181831-20.37538241229.49729095841-14.3965054998
141.858319634861.16679668892-1.9140612120.796968087267-1.647757157135.327269441290.0709531132869-0.6801772256870.08906930132720.8803118882120.619900306292-0.318575442768-0.5392693674640.497250712903-0.5814122410971.653425024271.17118836235-0.2347090458551.16084723671-0.211367457690.858261434635-15.659329155912.1948562765-4.26327853485
154.122847258741.954469374070.1910909146982.690079703422.625706360923.6494680683-0.748940749896-0.15424042311-0.429259152994-0.473413393630.40237153991.40397663252-0.631304507608-0.01171856067290.4223094402011.586485595150.462824579608-0.4526384355830.2753826053520.003475425868931.34595896739-7.3793394389611.4778019272-12.9434696601
165.057228685193.50538597384-1.946617858984.40060183256-4.206604938554.902427397811.203745120420.967356346090.938930296089-2.458304422310.751372295609-1.12630188447-1.5346309146-0.370237036146-1.57904494652.11963527121-0.3135754278550.3929047806290.703404544711-0.05826039992490.933822307168-3.1991944732916.9868570958-18.1130715458
172.608115426961.52615510952.431244967417.582410883912.883771367993.15852007319-0.190352421366-0.62076777407-0.034778683441-0.8086673181361.66869417973-0.802980938951-1.077360839020.813450796302-1.14441596880.8995289718620.06681472324760.06269536893520.931529288841-0.02968110696560.9632081527241.1506522096511.9636593487-2.2094157484
182.667597879460.4417495878082.011230726795.22838560264-5.440317840378.1779868382-1.148901545670.3066400107810.454894090619-0.0388201398707-1.04685060525-1.41607042199-0.176496979658-0.4104548071751.830291259881.017503444520.339384988263-0.07632469836490.645537054433-0.2172574705951.153838134313.496222678699.40421804032-1.4033663298
194.75144992485-3.66015346739-1.032864548552.904565286141.502796229537.41657511271-0.11515376062-0.6346065894110.4776873576340.4863528358160.347635971945-1.80001578883-0.95641036681.40648936391-0.09159025785790.842831611705-0.1201260463660.001599433424450.712400034209-0.05494469946271.06625465197-1.5704032132814.8292232756-1.04989054502
205.96722054732.13831294794-2.002844416073.91668850155-1.596644627322.61338782477-0.1146870216580.7948258026392.355770688410.2476117696430.0134625414091-0.5465741134030.3231960202820.1573296463530.2255574320461.19624167932-0.596320568647-0.2304142465391.32020631133-0.07137649472451.780134354465.7293566038624.4775000620.478330012421
219.35756488805-2.205711072172.419506050619.56447545402-0.9657768145680.680653403677-0.1448345276821.558223507870.0925799771074-0.6156462306560.188412585451-0.7560472270370.7928370956950.1128735548830.06216506744282.098285724870.3776154625060.02297339348881.40840748544-0.1710316202330.9371867045228.63162252994-29.8778146644-37.7598744983
228.05632174056-1.875473297620.5904588982153.030210517941.362191567165.575589986580.6881485297941.02819014505-2.368539993922.1731047645-0.7432579587650.9533339167180.5212122319540.4850020001450.1575642403162.074883724230.536482275894-0.1785175939561.00027940971-0.3026083439951.290796671765.48394653251-36.1838862719-30.1536962402
239.43614372680.490171761494-3.484768676390.8796260672080.06001253723772.452371624941.206611002851.419866633891.049684879930.02599285809460.440232216836-0.04378620701931.35683813188-0.854950786288-1.852469027841.796250886860.13794458876-0.2796940149191.217801916710.2984710446041.150447988946.43040381336-21.5498159385-40.566167857
246.800640510925.339346668475.571748931748.01178534562.722318433178.659170786830.6044807449951.25304662652.54284938167-0.0958809260381-1.061410780831.17145761381-0.2812411776510.338537394910.495095597622.602489389190.353001339218-0.1455369239870.8700342807620.1063365638411.18308133241.83889020748-20.5609045762-32.0924898682
255.722085417645.342646658410.2708851006488.50252878875.729561662268.60039438279-0.9308847796321.793432801370.8272555514540.5051927053030.06835773072411.05037656159-0.898808797906-2.057701159750.9318427813151.063266732550.591563151815-0.1391390622491.65507488466-0.2776470495960.990270386966-2.58054441947-25.2555881512-25.7520219286
266.663303147961.2223687806-0.2261099336662.216857349220.1577326336731.971079760552.17660708518-0.170762674581-0.196948350861-2.65166958186-1.736920429882.64104110883-1.116491562030.55854349809-0.3517274109111.72821166956-0.0875383194141-0.3700816852371.05894509641-0.002374081797021.261868922676.68434230348-25.0814639779-23.4856923106
273.44390052253-1.18945633754-1.068215886628.1498594373.125144484984.63409252114-0.7916985569590.0204185422708-0.613145994982-0.200687056716-0.6339789455220.53438814076-2.48982027868-0.1674785216561.25872750991.70324753876-0.0275496099412-0.001687410348491.20048469532-0.2211703648660.99393836817519.9627686032-29.3272778397-27.7965822071
285.440682611190.436442647286-4.292297753854.335200549472.943974906025.95570430661-2.757494992531.803997415061.312563687630.3626088594931.239309310730.6987862365971.271392694471.043006543611.297508046431.419617711290.395595564196-0.3154607940841.87736329072-0.1670428649611.6044609033314.8359037927-17.5608587612-28.5954614444
298.8505308438-4.56398193333.590432093232.36206209853-2.045406185715.48693188477-0.707274980740.6495553141380.116575049331-0.416231690715-1.13664096116-1.87118852254-0.1006273902880.7482607094711.174712281511.49784812027-1.021504526660.5748199660711.76214738875-0.2189591157871.8644488409416.6873139786-19.2980167852-36.4098424181
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787.768856356492.745994398171.562145371481.45726616228-1.572174743579.482176288261.01123386551.6500521424-0.856764504228-0.922543183101-1.457557782090.5279654057630.1721139129450.8182302054310.5608014821790.9549904244390.327128086959-0.1259175567251.40130974492-0.1709399707030.800194271494-36.572392982114.700062381730.7578587458
795.712330057761.93856207939-1.051514743670.674801772779-0.2611202305230.842443413614-0.2528655876610.25152855008-0.297378652272-0.418132908901-0.373883465928-0.0633429394801-1.08597935382-2.404391150650.57615265210.8363993656710.569195914970.002377071000412.06075003832-0.5434424340631.48878761654-43.483923888112.914177541430.8704371218
809.500293483-5.02351528931-7.162361689353.470340130332.474729503887.706835216220.0661578163510.602408665003-2.78970566726-0.364181398122-0.4896835251641.15619992220.287527676625-3.03458082250.0807389683650.4884430168890.0158504291643-0.3035857862111.200577500680.03396722410741.72553945948-29.205546623815.57875193321.1450564513
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1(chain A and resid 2:6)
2X-RAY DIFFRACTION2(chain A and resid 7:11)
3X-RAY DIFFRACTION3(chain A and resid 12:17)
4X-RAY DIFFRACTION4(chain A and resid 18:23)
5X-RAY DIFFRACTION5(chain A and resid 24:31)
6X-RAY DIFFRACTION6(chain A and resid 32:38)
7X-RAY DIFFRACTION7(chain A and resid 39:45)
8X-RAY DIFFRACTION8(chain A and resid 46:51)
9X-RAY DIFFRACTION9(chain A and resid 52:61)
10X-RAY DIFFRACTION10(chain A and resid 62:70)
11X-RAY DIFFRACTION11(chain A and resid 71:74)
12X-RAY DIFFRACTION12(chain A and resid 75:84)
13X-RAY DIFFRACTION13(chain A and resid 85:95)
14X-RAY DIFFRACTION14(chain A and resid 96:102)
15X-RAY DIFFRACTION15(chain A and resid 103:108)
16X-RAY DIFFRACTION16(chain A and resid 109:113)
17X-RAY DIFFRACTION17(chain A and resid 114:123)
18X-RAY DIFFRACTION18(chain A and resid 124:135)
19X-RAY DIFFRACTION19(chain A and resid 136:154)
20X-RAY DIFFRACTION20(chain A and resid 155:159)
21X-RAY DIFFRACTION21(chain B and resid 2:8)
22X-RAY DIFFRACTION22(chain B and resid 9:15)
23X-RAY DIFFRACTION23(chain B and resid 16:23)
24X-RAY DIFFRACTION24(chain B and resid 24:34)
25X-RAY DIFFRACTION25(chain B and resid 35:41)
26X-RAY DIFFRACTION26(chain B and resid 42:46)
27X-RAY DIFFRACTION27(chain B and resid 47:51)
28X-RAY DIFFRACTION28(chain B and resid 52:59)
29X-RAY DIFFRACTION29(chain B and resid 60:65)
30X-RAY DIFFRACTION30(chain B and resid 66:72)
31X-RAY DIFFRACTION31(chain B and resid 73:82)
32X-RAY DIFFRACTION32(chain B and resid 83:87)
33X-RAY DIFFRACTION33(chain B and resid 88:93)
34X-RAY DIFFRACTION34(chain B and resid 94:105)
35X-RAY DIFFRACTION35(chain B and resid 106:117)
36X-RAY DIFFRACTION36(chain B and resid 118:125)
37X-RAY DIFFRACTION37(chain B and resid 126:133)
38X-RAY DIFFRACTION38(chain B and resid 134:143)
39X-RAY DIFFRACTION39(chain B and resid 144:150)
40X-RAY DIFFRACTION40(chain B and resid 151:156)
41X-RAY DIFFRACTION41(chain C and resid 2:15)
42X-RAY DIFFRACTION42(chain C and resid 16:21)
43X-RAY DIFFRACTION43(chain C and resid 22:31)
44X-RAY DIFFRACTION44(chain C and resid 32:37)
45X-RAY DIFFRACTION45(chain C and resid 38:46)
46X-RAY DIFFRACTION46(chain C and resid 47:52)
47X-RAY DIFFRACTION47(chain C and resid 53:57)
48X-RAY DIFFRACTION48(chain C and resid 58:64)
49X-RAY DIFFRACTION49(chain C and resid 65:75)
50X-RAY DIFFRACTION50(chain C and resid 76:83)
51X-RAY DIFFRACTION51(chain C and resid 84:91)
52X-RAY DIFFRACTION52(chain C and resid 92:98)
53X-RAY DIFFRACTION53(chain C and resid 99:105)
54X-RAY DIFFRACTION54(chain C and resid 106:111)
55X-RAY DIFFRACTION55(chain C and resid 112:120)
56X-RAY DIFFRACTION56(chain C and resid 121:126)
57X-RAY DIFFRACTION57(chain C and resid 127:136)
58X-RAY DIFFRACTION58(chain C and resid 137:143)
59X-RAY DIFFRACTION59(chain C and resid 144:154)
60X-RAY DIFFRACTION60(chain C and resid 155:159)
61X-RAY DIFFRACTION61(chain D and resid 4:8)
62X-RAY DIFFRACTION62(chain D and resid 9:14)
63X-RAY DIFFRACTION63(chain D and resid 15:20)
64X-RAY DIFFRACTION64(chain D and resid 21:27)
65X-RAY DIFFRACTION65(chain D and resid 28:36)
66X-RAY DIFFRACTION66(chain D and resid 37:44)
67X-RAY DIFFRACTION67(chain D and resid 45:51)
68X-RAY DIFFRACTION68(chain D and resid 52:57)
69X-RAY DIFFRACTION69(chain D and resid 58:65)
70X-RAY DIFFRACTION70(chain D and resid 66:72)
71X-RAY DIFFRACTION71(chain D and resid 73:77)
72X-RAY DIFFRACTION72(chain D and resid 78:84)
73X-RAY DIFFRACTION73(chain D and resid 85:90)
74X-RAY DIFFRACTION74(chain D and resid 91:97)
75X-RAY DIFFRACTION75(chain D and resid 98:103)
76X-RAY DIFFRACTION76(chain D and resid 104:115)
77X-RAY DIFFRACTION77(chain D and resid 116:123)
78X-RAY DIFFRACTION78(chain D and resid 124:133)
79X-RAY DIFFRACTION79(chain D and resid 134:147)
80X-RAY DIFFRACTION80(chain D and resid 148:156)

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