PDB-6oar: Structure of the Kupe virus OTU bound to the C-terminal domain of...

Basic information

• Entry: Database: PDB / ID: 6oar
• Title: Structure of the Kupe virus OTU bound to the C-terminal domain of sheep ISG15

Components

• Interferon stimulated gene 17
• RNA-dependent RNA polymerase

• Keywords: HYDROLASE / DUB / deISGylase / Innate Immunity

Function / homology

Function:

RNA-directed RNA polymerase / viral RNA genome replication / RNA-directed RNA polymerase activity / DNA-templated transcription

Domain/homology:

: / Cathepsin B; Chain A - #80 / OTU-like cysteine protease / RNA-directed RNA polymerase L, N-terminal / L protein N-terminus / OTU domain / OTU domain profile. / RNA-dependent RNA polymerase, bunyaviral / Bunyavirus RNA dependent RNA polymerase / RNA-directed RNA polymerase, negative-strand RNA virus / RdRp of negative ssRNA viruses with segmented genomes catalytic domain profile. / Cathepsin B; Chain A / Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1 / Ubiquitin-like (UB roll) / Ubiquitin family / Ubiquitin homologues / Ubiquitin domain profile. / Ubiquitin-like domain / Ubiquitin-like domain superfamily / Roll / Alpha-Beta Complex / Alpha Beta

Component:

prop-2-en-1-amine / RNA-directed RNA polymerase L / Interferon stimulated gene 17

• Biological species: Kupe virus; Ovis aries (sheep)
• Method: X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.063 Å
• Authors: Dzimianski, J.V. / Williams, I.L. / Pegan, S.D.

Funding support

United States, 1items

Organization	Grant number	Country
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)	1R01AI109008	United States

• Citation: Journal: Plos One / Year: 2019; Title: Determining the molecular drivers of species-specific interferon-stimulated gene product 15 interactions with nairovirus ovarian tumor domain proteases.; Authors: Dzimianski, J.V. / Scholte, F.E.M. / Williams, I.L. / Langley, C. / Freitas, B.T. / Spengler, J.R. / Bergeron, E. / Pegan, S.D.

History

Deposition	Mar 18, 2019	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Jan 8, 2020	Provider: repository / Type: Initial release
Revision 1.1	Oct 11, 2023	Group: Data collection / Database references / Derived calculations / Refinement description Category: chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_conn Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id

Assembly

Deposited unit

A: RNA-dependent RNA polymerase

B: Interferon stimulated gene 17

C: RNA-dependent RNA polymerase

D: Interferon stimulated gene 17

E: RNA-dependent RNA polymerase

F: Interferon stimulated gene 17

G: RNA-dependent RNA polymerase

H: Interferon stimulated gene 17

hetero molecules

Summary:

• 117 kDa, 8 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	117,464	12
Polymers	117,236	8
Non-polymers	228	4
Water	6,467	359

1

A: RNA-dependent RNA polymerase

B: Interferon stimulated gene 17

hetero molecules

Summary:

• defined by author&software
• 29.4 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	29,366	3
Polymers	29,309	2
Non-polymers	57	1
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	2100 Å2
ΔGint	-5 kcal/mol
Surface area	11260 Å2
Method	PISA

2

C: RNA-dependent RNA polymerase

D: Interferon stimulated gene 17

hetero molecules

Summary:

• defined by author&software
• 29.4 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	29,366	3
Polymers	29,309	2
Non-polymers	57	1
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	2120 Å2
ΔGint	-5 kcal/mol
Surface area	10990 Å2
Method	PISA

3

E: RNA-dependent RNA polymerase

F: Interferon stimulated gene 17

hetero molecules

Summary:

• defined by author&software
• 29.4 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	29,366	3
Polymers	29,309	2
Non-polymers	57	1
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	2100 Å2
ΔGint	-7 kcal/mol
Surface area	10990 Å2
Method	PISA

4

G: RNA-dependent RNA polymerase

H: Interferon stimulated gene 17

hetero molecules

Summary:

• defined by author&software
• 29.4 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	29,366	3
Polymers	29,309	2
Non-polymers	57	1
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	2100 Å2
ΔGint	-4 kcal/mol
Surface area	11330 Å2
Method	PISA

Unit cell

Length a, b, c (Å)	41.796, 158.758, 171.040
Angle α, β, γ (deg.)	90.00, 90.00, 90.00
Int Tables number	19
Space group name H-M	P212121

Components

#1: Protein

A C E G
RNA-dependent RNA polymerase

Details:

Mass: 20392.881 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Kupe virus / Production host: Escherichia coli (E. coli) / References: UniProt: B8PWH5

#2: Protein

B D F H
Interferon stimulated gene 17

Details:

Mass: 8916.149 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Ovis aries (sheep) / Gene: ISG17 / Production host: Escherichia coli (E. coli) / References: UniProt: Q9GKP4

#3: Chemical

A-201 C-201 E-201 G-201
ChemComp-AYE / prop-2-en-1-amine / ALLYLAMINE

Details:

Mass: 57.094 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Formula: C₃H₇N

#4: Water

ChemComp-HOH / water

Details:

Mass: 18.015 Da / Num. of mol.: 359 / Source method: isolated from a natural source / Formula: H₂O

Experimental details

Experiment

• Experiment: Method: X-RAY DIFFRACTION / Number of used crystals: 1

Sample preparation

• Crystal: Density Matthews: 2.54 ų/Da / Density % sol: 51.55 %
• Crystal grow: Temperature: 293 K / Method: vapor diffusion, hanging drop; Details: 0.1 M sodium acetate pH 4.1, 2.2 M potassium acetate

Data collection

• Diffraction: Mean temperature: 100 K / Serial crystal experiment: N
• Diffraction source: Source: SYNCHROTRON / Site: NSLS-II / Beamline: 17-ID-1 / Wavelength: 0.92 Å
• Detector: Type: DECTRIS EIGER X 9M / Detector: PIXEL / Date: Jul 20, 2018
• Radiation: Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
• Radiation wavelength: Wavelength: 0.92 Å / Relative weight: 1
• Reflection: Resolution: 2.06→50 Å / Num. obs: 70403 / % possible obs: 99.8 % / Redundancy: 5.1 % / CC_1/2: 0.994 / Net I/σ(I): 15.9
• Reflection shell: Resolution: 2.06→2.11 Å / Redundancy: 4.6 % / Mean I/σ(I) obs: 1.51 / Num. unique obs: 6137 / CC_1/2: 0.699 / % possible all: 99.9

Processing

Software

Name	Version	Classification
PHENIX	(1.11.1_2575: ???)	refinement
HKL-2000		data reduction
HKL-2000		data scaling
PHASER		phasing

Refinement

Method to determine structure: MOLECULAR REPLACEMENT
Starting model: composite homology model generated from PDB entries 4HXD, 5JZE, 3PSE, and 3PHX

4hxd

5jze

3pse

3phx

Resolution: 2.063→41.289 Å / SU ML: 0.22 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 24.54

	Rfactor	Num. reflection	% reflection
Rfree	0.2166	3510	4.99 %
Rwork	0.1764	-	-
obs	0.1785	70368	98.51 %

• Solvent computation: Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å

Refinement step

Cycle: LAST / Resolution: 2.063→41.289 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	7512	0	16	359	7887

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.003	7711
X-RAY DIFFRACTION	f_angle_d	0.543	10429
X-RAY DIFFRACTION	f_dihedral_angle_d	15.509	4569
X-RAY DIFFRACTION	f_chiral_restr	0.047	1161
X-RAY DIFFRACTION	f_plane_restr	0.004	1350

LS refinement shell

Resolution (Å)	Rfactor Rfree	Num. reflection Rfree	Rfactor Rwork	Num. reflection Rwork	Refine-ID	% reflection obs (%)
2.0627-2.091	0.3056	102	0.2613	1831	X-RAY DIFFRACTION	68
2.091-2.1208	0.3008	120	0.2527	2640	X-RAY DIFFRACTION	100
2.1208-2.1525	0.3254	129	0.2533	2644	X-RAY DIFFRACTION	100
2.1525-2.1861	0.2708	126	0.2329	2727	X-RAY DIFFRACTION	100
2.1861-2.222	0.2963	160	0.2281	2663	X-RAY DIFFRACTION	100
2.222-2.2603	0.2737	131	0.2371	2632	X-RAY DIFFRACTION	100
2.2603-2.3014	0.282	139	0.2147	2721	X-RAY DIFFRACTION	100
2.3014-2.3456	0.2512	127	0.2122	2689	X-RAY DIFFRACTION	100
2.3456-2.3935	0.2574	137	0.2057	2633	X-RAY DIFFRACTION	100
2.3935-2.4455	0.2673	132	0.2023	2751	X-RAY DIFFRACTION	100
2.4455-2.5024	0.2398	132	0.1969	2665	X-RAY DIFFRACTION	100
2.5024-2.565	0.2612	149	0.1983	2681	X-RAY DIFFRACTION	100
2.565-2.6343	0.2407	135	0.2039	2721	X-RAY DIFFRACTION	100
2.6343-2.7118	0.2379	150	0.2053	2651	X-RAY DIFFRACTION	100
2.7118-2.7994	0.2463	133	0.2119	2750	X-RAY DIFFRACTION	100
2.7994-2.8994	0.2739	135	0.2057	2671	X-RAY DIFFRACTION	100
2.8994-3.0154	0.2723	127	0.2028	2736	X-RAY DIFFRACTION	100
3.0154-3.1526	0.2796	157	0.1881	2692	X-RAY DIFFRACTION	100
3.1526-3.3188	0.2227	132	0.167	2717	X-RAY DIFFRACTION	100
3.3188-3.5266	0.2172	140	0.1608	2753	X-RAY DIFFRACTION	100
3.5266-3.7987	0.1763	151	0.1559	2740	X-RAY DIFFRACTION	100
3.7987-4.1807	0.1631	164	0.1312	2717	X-RAY DIFFRACTION	100
4.1807-4.7848	0.1447	149	0.1213	2723	X-RAY DIFFRACTION	99
4.7848-6.0254	0.1907	177	0.1473	2784	X-RAY DIFFRACTION	100
6.0254-41.2968	0.1988	176	0.1802	2926	X-RAY DIFFRACTION	99

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°2)	L12 (°2)	L13 (°2)	L22 (°2)	L23 (°2)	L33 (°2)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å2)	T12 (Å2)	T13 (Å2)	T22 (Å2)	T23 (Å2)	T33 (Å2)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	7.6667	4.7254	-1.8617	3.508	-0.2234	2.0832	-0.4435	0.1086	0.3918	-0.2187	-0.0423	0.1121	-0.4607	-0.8237	0.4886	0.3131	0.0058	0.0272	0.5052	0.0601	0.4836	-9.8179	11.2023	-15.4766
2	3.2333	0.3013	2.8702	5.7819	-1.9595	3.4195	-0.3652	-0.3435	0.3415	1.5903	0.2449	0.3547	-0.6263	0.0409	0.2009	0.6424	0.0272	0.0435	0.4481	0.0065	0.3176	0.6957	13.0671	-4.6702
3	8.8465	1.2605	1.0655	8.5507	3.9841	2.1598	-0.2702	-0.2228	0.2907	0.6534	0.0942	0.5675	-0.7576	-0.3259	0.1112	0.4893	0.0498	-0.0082	0.2883	0.0804	0.2465	0.6837	12.0721	-9.5514
4	8.9123	-0.4407	-1.3605	4.3101	-4.0806	4.6504	0.5566	1.3761	-0.6738	-1.0177	0.5369	0.8258	2.3404	-0.9422	-1.0039	0.4882	-0.138	-0.1312	0.505	0.0125	0.4866	-3.7736	8.4012	-25.3349
5	6.202	5.4681	-5.6938	5.0509	-4.7864	5.3998	-0.6719	0.8346	-0.3404	-0.4309	0.9802	0.2638	0.1013	-1.625	-0.3533	0.186	0.0009	-0.0191	0.5336	0.091	0.3739	-2.3212	17.242	-28.2784
6	2.8556	0.174	-0.7476	3.362	-0.0236	5.4947	0.0574	0.0469	0.1146	0.2939	0.0227	-0.329	-0.6485	0.0802	-0.1551	0.2666	-0.0143	-0.0256	0.2128	0.0568	0.2267	10.0482	25.4526	-24.6644
7	2.9494	4.1652	-2.5155	8.0299	-7.3863	9.6917	-0.3042	0.2058	0.515	-0.0019	0.4509	0.2514	-0.451	-0.7942	-0.2749	0.5205	0.1125	0.091	0.303	0.0924	0.3806	1.9613	32.2141	-24.9943
8	2.5207	0.3037	2.1761	5.1741	5.9518	8.1978	0.9865	-0.0885	0.9368	1.1043	0.5994	-0.1769	-1.5426	0.4101	-1.1692	0.5203	-0.0282	0.135	0.2402	0.0223	0.465	10.7305	34.5824	-24.3115
9	7.3176	1.0189	-0.006	6.7369	3.5292	6.6081	0.3038	-0.5039	1.7786	0.6972	-0.1167	-0.1306	-1.7558	0.2508	-0.2014	0.7095	-0.135	0.0178	0.2883	-0.0732	0.4627	11.6376	33.7385	-14.1193
10	2.8467	-3.5153	0.5307	7.5214	1.7126	7.3332	0.5435	-0.5783	0.8047	0.7846	-0.6007	-0.7801	-1.7651	1.1492	-0.2477	1.0619	-0.1908	-0.1573	0.4511	-0.12	0.4279	13.0103	29.1785	-4.8926
11	1.9156	2.6868	2.7736	5.4428	5.0265	4.7936	-0.0134	-0.2963	0.3382	1.3063	-0.0478	-0.5884	0.8623	1.3318	0.1043	0.8927	-0.0444	-0.1786	0.5479	0.0071	0.369	13.552	21.2043	-3.5496
12	9.2333	-1.1725	1.2594	0.6918	0.7985	3.6235	0.0801	-1.4679	-0.6294	0.8505	0.3405	-0.1232	0.9189	1.2044	-0.4482	0.9233	-0.2302	-0.5441	1.1936	0.1703	0.5745	21.1432	22.1189	-4.1466
13	9.6219	3.1096	5.0816	2.7207	3.1676	4.0402	-0.5833	-2.0936	0.55	0.6767	0.3382	-0.5367	-0.9003	1.1346	0.4265	0.7311	-0.2266	-0.2224	0.8355	-0.186	0.7229	21.9137	29.0727	-9.982
14	9.5671	1.4666	-5.3926	4.4722	-1.0016	3.0838	0.4471	-0.2481	0.6632	0.3689	-0.3372	-0.5044	-1.6434	0.8431	-0.0642	0.5458	-0.1713	-0.0866	0.5164	0.0417	0.458	18.4932	26.354	-20.0685
15	6.1455	-1.5122	-1.5188	7.2499	1.2525	5.5118	0.2796	-0.4617	0.2543	0.9757	-0.2679	-0.145	-0.4108	0.1026	-0.0314	0.6301	-0.0565	-0.0554	0.3182	-0.0134	0.3177	8.4435	24.1993	-10.2811
16	5.8188	1.6704	1.3637	4.1473	-2.7309	3.3649	0.0119	0.0624	0.2533	0.4288	0.0539	0.2449	-0.4969	-0.1718	-0.0245	0.2691	0.037	0.0207	0.2027	0.0656	0.1559	2.2336	20.1221	-15.5194
17	2.6495	1.0598	-4.0589	7.1787	-1.7496	9.1025	-0.3143	0.9499	-0.85	-1.4625	0.1578	-1.4999	0.2829	0.9219	0.2553	0.5593	-0.0288	0.1518	0.5308	-0.0907	0.4436	3.4772	6.8157	-24.0604
18	2.1791	3.446	2.0355	8.2063	1.9939	2.552	0.0667	0.3751	0.6172	0.4906	0.0627	0.8002	-0.5559	-1.1369	-0.1505	0.4517	0.0663	0.1053	0.4991	0.065	0.3013	-2.9866	18.8765	-11.7276
19	3.0078	-2.8466	-3.6876	7.4676	0.9465	6.0204	-0.0211	0.0024	-0.2534	0.1573	-0.0608	0.0187	-0.0418	-0.0461	0.0267	0.2851	0.0327	-0.0045	0.2867	0.0183	0.2444	5.6534	17.4851	-22.4187
20	7.5502	6.298	5.5019	5.2754	4.5936	3.9954	-0.235	-0.3467	2.4154	-0.236	-0.1215	1.3761	-0.6159	-1.4471	0.0656	0.2273	0.1476	0.0567	0.7206	0.1299	0.514	-7.1976	19.7745	-25.3438
21	5.2725	-5.1165	6.6959	5.0262	-6.5325	9.1151	0.6374	-0.3137	-1.0496	-0.1066	-0.1359	-0.758	0.9889	1.5621	-0.2654	0.5288	0.0677	-0.0745	0.5051	0.0837	0.4849	12.1053	-4.7443	2.1963
22	3.5557	-3.1858	2.4765	3.2589	-0.9131	8.4862	0.3683	0.2894	0.0172	-0.3156	-0.2286	-0.2856	0.5054	0.0286	-0.1906	0.343	-0.014	-0.0532	0.2636	0.0142	0.278	6.3935	0.7406	-11.1961
23	7.6942	4.9137	5.7155	3.155	3.6796	4.4935	0.6153	-0.1296	-2.4089	-0.5308	0.3867	-1.406	0.9673	0.1421	-0.8173	0.5593	0.0079	-0.1099	0.3828	0.0062	0.4922	15.6651	-5.0187	0.1853
24	5.5279	1.9499	-2.3682	5.0563	0.6243	1.9975	0.0272	-0.5775	-0.7156	0.8808	-0.1112	-0.7384	-0.6288	-0.2668	-0.0135	0.4441	-0.0041	-0.1393	0.41	0.0341	0.2917	20.0931	2.5402	4.0178
25	4.2978	-2.2715	2.0317	4.2886	2.0551	4.1118	-0.0372	0.3258	-0.3463	0.3811	0.1148	-0.6876	0.6419	0.9108	-0.0086	0.4085	0.0213	-0.0454	0.3582	0.0061	0.3785	20.2016	1.7965	-4.2001
26	3.4401	2.2225	-2.336	9.8626	-2.4637	2.0852	0.0211	0.4303	-0.4206	-0.3101	-0.4232	-0.6816	-0.08	0.6602	0.4362	0.398	0.0767	-0.0203	0.437	-0.0339	0.4091	17.2814	-1.3301	-10.7167
27	8.604	3.3598	2.5155	5.6785	5.3679	5.2836	-0.1746	-0.0901	-0.8546	-0.8691	0.2683	-2.7656	0.4656	0.9824	-0.0545	0.5952	-0.0107	0.1067	0.5576	0.0268	0.4867	19.9233	5.0042	-13.8133
28	9.9988	-0.3325	-1.3158	2.0843	-1.8726	6.5942	-0.0794	0.8007	0.2344	-0.5968	-0.0688	0.2632	-0.0317	0.1184	-0.0073	0.4458	-0.0052	-0.0734	0.2984	0.0132	0.2793	13.9296	9.608	-7.3211
29	3.019	-0.5928	-3.0759	1.5693	-2.1258	8.1506	0.261	-1.3311	0.0915	1.6325	-0.2633	-0.1209	-1.1564	-0.3472	-0.0105	0.4616	-0.0058	-0.0699	0.2994	-0.0301	0.212	8.0666	10.1305	3.0878
30	4.2047	-2.6356	3.6766	2.5826	-1.7724	3.507	-0.8742	0.4589	0.8974	-0.1666	-0.0341	-0.7036	-1.0242	0.3617	0.8398	0.5856	-0.1005	-0.1697	0.4092	0.0457	0.4761	19.525	10.7214	2.2135
31	7.8657	-5.8853	0.3707	4.5789	-0.9088	2.1533	0.2943	0.2711	-0.1985	0.3648	-0.5685	-0.9293	-0.1149	0.1375	0.1531	0.4503	-0.0008	-0.157	0.4697	0.0247	0.3605	15.7928	6.6543	7.2242
32	3.3614	2.0644	-3.0898	4.0188	-1.0385	4.9989	-0.3158	-0.1562	-0.1275	0.1359	-0.0673	-0.3469	0.479	-0.4594	0.3954	0.4359	0.0095	-0.0986	0.3143	0.0262	0.3188	8.1728	1.6664	0.9331
33	2.3877	3.5782	-3.7691	5.4657	-5.7117	5.9167	-0.231	-0.114	-0.1988	0.4772	-0.625	-1.0624	-0.1897	0.9055	0.9493	0.4183	0.0112	-0.0684	0.407	0.0949	0.3432	13.3666	14.3439	-13.7877
34	2.1957	1.5373	0.7089	4.3293	4.191	4.5757	0.3179	0.3562	-1.6959	0.5809	-0.0819	-0.8612	1.0516	1.9435	-0.0748	0.4017	0.1374	0.0556	0.5553	0.0844	0.7021	20.7172	2.7708	-47.0874
35	9.4933	-3.4232	5.5661	2.2659	-2.0462	3.2727	-0.1234	1.4697	-0.4557	-0.823	-0.2473	-1.0313	-0.0188	0.8453	0.4526	0.407	-0.0079	0.123	0.4064	-0.0233	0.4059	11.8854	5.9278	-57.3306
36	2.5898	-0.4534	1.5072	6.444	-1.6705	6.2479	0.025	0.4368	-0.2397	-0.0995	-0.2441	-0.4048	-0.46	0.3551	0.0789	0.2938	-0.0382	0.032	0.3102	-0.0023	0.2834	10.2839	7.8242	-54.7011
37	5.6068	6.2384	5.805	7.8851	6.8394	6.1692	0.6234	-1.4404	-0.8696	1.1173	0.1999	-0.9053	1.4711	0.0666	-0.5966	0.4066	0.1456	0.0026	0.3883	0.0227	0.4547	15.0038	5.2695	-38.4942
38	5.5051	-5.2098	-4.2429	5.2828	4.1488	3.3208	-0.6773	-0.2394	-0.1002	2.1461	0.4471	-0.6105	0.0034	0.1512	0.2983	0.3444	0.098	0.0048	0.3825	0.0066	0.3745	14.214	17.2752	-34.5463
39	7.9341	-1.5768	1.67	7.3428	0.599	3.8546	-0.5059	0.3416	0.4027	-0.1367	0.3039	-0.2804	-0.3377	0.295	0.2001	0.3246	-0.036	0.0167	0.2208	-0.0007	0.3246	12.2456	25.3454	-41.8533
40	3.8803	-4.4926	-3.5413	5.2463	4.0745	3.2286	0.2678	-0.2536	1.2291	0.105	0.3713	-2.068	-0.9404	1.6339	-0.5484	0.5959	-0.0716	-0.145	0.4221	-0.0651	0.7457	18.1563	30.7311	-37.8279
41	5.5921	0.1828	0.076	5.8199	3.2855	4.4126	0.2726	-0.0012	1.7438	-0.5009	0.6928	-0.034	-1.332	0.617	-0.5077	0.417	-0.0363	0.111	0.2188	-0.0586	0.4986	13.1516	32.4174	-43.8213
42	4.4792	-0.1603	-3.2774	2.2137	0.9189	4.003	-0.0999	-0.0193	2.0494	-0.2413	0.6517	-0.7092	-1.56	0.5151	-0.5066	0.6294	-0.0764	0.1298	0.3485	-0.0226	0.5539	11.4457	32.5955	-51.8671
43	8.3984	1.911	2.1638	4.6461	0.6919	3.4086	0.2622	0.4808	0.1548	-1.8241	-0.2112	-0.5703	-0.2316	0.1173	0.0265	0.829	-0.0445	0.1083	0.3162	0.0402	0.3284	7.5243	26.5853	-61.4203
44	8.4821	-6.4189	-3.0722	4.8701	2.377	1.184	0.6495	0.5852	-0.9707	-0.5665	-1.1998	1.0634	-0.1073	-0.7529	0.7338	0.5932	0.0273	-0.0617	0.7205	-0.0958	0.4709	-2.4433	22.3682	-59.8702
45	4.6976	-2.0163	3.7476	8.6248	1.7772	4.4821	-0.9718	0.5182	-0.2471	-0.3258	0.4742	0.6144	-1.1578	-0.1841	0.207	0.8563	0.1645	-0.1499	0.4577	0.0115	0.4979	0.1547	31.857	-58.9936
46	9.6546	2.4831	3.1799	1.7053	-0.0399	6.8472	-0.3347	-0.5839	0.9708	-0.5491	0.0161	-0.0976	-1.3565	-0.4562	0.2252	0.7389	0.1717	-0.0083	0.3269	0.0494	0.3247	2.1961	33.9734	-51.0504
47	1.9216	1.0614	0.8253	5.7214	6.2817	7.0121	0.03	-0.067	0.6836	-1.0652	-0.1753	0.7614	-2.4717	-0.4213	0.0305	0.4404	0.0437	-0.0074	0.3137	-0.0245	0.3504	1.4945	28.4802	-43.582
48	5.2186	2.1305	-1.2639	5.3727	-1.4231	5.0883	-0.168	0.0861	-0.2275	-0.7538	-0.124	-0.542	-0.3599	0.4955	0.3008	0.451	-0.042	0.0799	0.2598	0.0234	0.3154	11.0762	23.0224	-54.9887
49	2.5404	1.1235	0.3569	7.8461	1.1599	2.3259	-0.232	0.7432	0.3046	-0.7783	0.3944	-0.5509	-0.2364	-0.0439	-0.0819	0.2048	0.0325	0.0664	0.2638	0.0082	0.3008	14.1287	15.2765	-50.0496
50	4.94	-3.5487	2.7511	7.9802	1.5176	3.8215	-0.1677	-3.4182	-1.3995	3.1161	0.0484	0.9738	-0.7753	0.3007	0.1032	0.6602	-0.0216	0.2195	0.8012	0.1476	0.4662	5.1378	5.9121	-36.9113
51	7.9918	4.1428	-4.4288	7.0806	-4.7341	3.7114	-0.2479	-0.2433	-0.2236	-0.3678	0.0737	-0.1928	0.5871	0.1835	0.0611	0.2987	0.0092	0.0594	0.2029	-0.0112	0.228	8.8609	8.3262	-48.6553
52	2.1509	-0.7523	-1.7569	4.6496	1.4617	7.0444	0.028	-0.2818	-0.171	-0.1479	-0.0538	-0.2876	-0.0173	0.3799	0.0385	0.1783	0.0119	0.0525	0.2594	0.008	0.2909	12.0124	14.6966	-46.0432
53	2.9641	-3.1208	0.21	3.5366	1.1278	7.9985	-0.5164	-0.3597	1.7798	0.4296	0.6131	-1.5223	0.0034	0.5145	-0.1644	0.2097	0.0392	-0.0301	0.3602	-0.0403	0.4754	17.8033	14.3869	-39.101
54	3.8438	1.5305	5.3815	2.4849	0.5047	9.6341	0.4058	0.7125	-0.7481	-0.2575	0.0645	0.4847	-0.2687	-0.7561	-0.3637	0.259	0.1153	-0.0361	0.5294	-0.0846	0.3496	-6.7974	-0.5707	-63.3421
55	3.1436	2.1705	2.2881	3.0045	-1.0395	7.0082	0.1366	-0.269	0.615	1.2907	0.1037	-0.0301	0.2999	-0.5612	-0.2597	0.259	0.0389	0.0257	0.315	-0.0421	0.3518	0.0956	1.1731	-50.9856
56	4.6852	-2.9217	5.563	2.3936	-3.2091	6.8443	-0.0045	0.341	-0.1064	-0.103	0.2265	0.6494	0.5966	-1.5263	-0.054	0.2591	0.0422	-0.034	0.4782	0.0002	0.3214	-9.9999	-0.9019	-60.2388
57	7.2694	-1.2525	3.2741	3.2192	4.4264	2.0006	-0.1129	-1.0083	-0.0359	-0.8158	-0.3178	1.1236	-0.3003	-1.3002	0.5704	0.3908	0.0904	-0.0404	0.686	0.0403	0.3256	-14.1576	6.9756	-67.8908
58	1.2035	1.1116	0.4531	1.9874	-0.4102	0.897	0.5509	-0.0197	0.0389	-0.2512	-0.5336	1.005	-0.4719	-0.5332	-0.1088	-0.9738	0.8507	-0.1361	0.2585	-0.0505	0.3469	-11.5636	9.4082	-59.1887
59	2.5502	-1.3213	-2.7203	8.1445	1.8546	3.0788	-0.6209	-0.6911	-0.5133	0.6381	0.4018	0.5064	0.1031	-0.6155	0.1637	0.2496	0.0726	0.0756	0.5715	-0.0427	0.4042	-10.478	4.5311	-52.0238
60	9.2978	4.743	-7.0856	3.295	-4.5476	7.928	0.3803	-1.1111	0.7785	0.8896	-0.1023	1.5885	-1.5521	-0.4841	-0.3642	0.4288	0.1781	0.008	0.6109	-0.0836	0.399	-9.5995	12.0815	-49.2645
61	2.4945	3.8381	-0.2954	6.6598	1.8214	7.2764	-0.0893	-0.6734	0.1685	-0.323	-0.4405	0.3406	-0.8005	-0.4092	0.4312	0.3075	0.1096	0.0353	0.3102	0.0026	0.304	-2.9668	12.7876	-55.5158
62	7.8331	2.762	0.0696	1.3035	-0.791	1.8993	0.1628	1.4822	-0.2562	-1.3917	0.1857	-0.4956	-0.875	0.7035	-0.315	0.4942	0.0687	0.0741	0.4671	-0.0226	0.2045	1.8119	9.0059	-66.5964
63	6.0355	-5.9608	5.8515	6.03	-5.904	5.7972	-0.629	-0.0022	1.193	0.0554	0.2616	-0.3037	-0.6894	-1.1096	0.1834	0.5387	0.18	-0.0525	0.5197	0.044	0.5132	-7.5678	15.7975	-65.5184
64	2.0965	1.0147	1.0764	3.6421	3.53	3.423	-0.1379	-0.0724	0.7721	-1.0749	0.2664	0.4519	-0.1336	-0.2449	0.0439	0.4253	0.0741	-0.0522	0.6087	0.0643	0.3402	-6.6771	9.6828	-70.5804
65	1.0499	0.912	1.5342	8.4586	5.1679	8.069	0.1169	0.2919	-0.0626	-0.6368	-0.1574	0.1853	-0.3916	-0.1915	0.0999	0.2239	0.0807	-0.0168	0.3191	-0.0206	0.2334	-1.7361	2.9037	-62.9647
66	9.4358	3.6105	6.051	7.563	4.8947	5.2403	-0.8055	-0.3729	0.5009	-0.7999	-0.1083	0.4895	-1.2683	-0.6924	1.0024	0.3176	0.1029	-0.0107	0.3786	-0.053	0.2483	0.6062	17.6491	-49.6655
67	7.4126	-3.9309	0.5406	3.4014	-3.0397	5.7664	0.011	0.378	-0.0699	2.155	0.1768	2.965	-0.8785	-1.9191	0.0602	0.5597	0.0562	0.2488	0.5401	-0.0296	0.7628	-16.3527	-8.3057	-45.977
68	8.4192	-2.4378	-2.8665	6.6507	-2.1839	8.4141	0.0018	0.7401	-0.2076	-0.6723	-0.0646	0.7947	0.4038	-1.0028	0.0908	0.2216	-0.0477	-0.0728	0.371	-0.0165	0.3597	-7.5046	-14.4673	-57.3527
69	5.8799	-2.4088	-2.0473	5.2685	-3.5046	5.0827	0.426	0.3798	1.2707	0.8174	-0.4402	0.3126	-0.8052	0.298	0.0171	0.3346	-0.0523	0.0623	0.274	-0.0929	0.4454	-5.2972	-7.2343	-45.6262
70	2.848	2.0599	0.2345	3.9034	-2.6351	3.2719	0.3442	-1.165	1.0664	2.186	-0.3643	1.2489	-0.9922	-1.3717	0.0444	0.5679	-0.0246	0.1921	0.4986	-0.0676	0.5225	-10.1529	-9.4453	-36.3545
71	5.6517	-6.0178	5.5592	6.7853	-6.4704	6.3086	-0.4364	-1.1315	0.5353	1.701	0.6969	0.5933	-0.6192	-1.5113	-0.0239	0.642	-0.002	0.1384	0.4201	-0.0605	0.4486	-9.977	-19.0496	-33.9222
72	6.7169	-1.2934	1.3809	5.776	0.9198	5.0517	-0.0951	-0.3106	-0.5929	0.9517	-0.1785	0.2694	0.3598	-0.0726	0.2987	0.3689	-0.0837	0.0518	0.2169	-0.0152	0.3527	-4.4054	-29.7383	-38.7258
73	7.0781	-6.0868	1.5839	6.5391	0.78	3.9073	-0.6497	-0.6088	-0.9584	0.8398	0.703	2.084	1.2204	-1.606	-0.0372	0.3861	-0.1961	0.0548	0.5078	0.0095	0.6674	-16.1046	-31.4567	-40.6193
74	6.9279	-0.3217	-1.0809	3.8385	-3.571	9.7975	0.3098	-0.0825	-0.4581	0.296	-0.448	0.314	1.6245	-0.483	0.1404	0.5505	-0.1135	0.02	0.2001	-0.0407	0.433	-7.656	-37.3228	-40.2016
75	6.1942	2.0638	-0.8821	4.7241	-1.8339	5.6173	0.0225	0.2898	-0.5452	-0.7859	-0.0673	0.2343	0.3428	0.1215	0.0225	0.4884	-0.0372	-0.0356	0.2234	-0.072	0.3428	-3.9098	-34.3756	-57.1273
76	9.7164	-1.2194	-3.991	2.1093	2.6177	9.9394	-0.1167	-0.1512	-0.152	-0.4783	-0.1263	-0.5375	-0.3596	1.2866	0.3178	0.4765	-0.0328	0.1291	0.4888	-0.0282	0.5721	5.6952	-25.9328	-59.452
77	9.1903	5.9518	4.3372	4.3198	4.1435	9.3336	0.0076	1.0732	-1.4448	0.4865	0.7095	-1.1224	0.3742	0.9014	-0.6254	0.4223	0.0368	0.0601	0.351	-0.1423	0.443	6.3327	-37.54	-58.0421
78	9.2028	6.7658	-1.8769	8.2028	-3.4455	1.8908	-0.4224	-0.0257	-0.2246	0.3784	0.1658	-0.3958	1.4575	0.0354	0.3334	0.4689	0.0679	0.0354	0.254	-0.0923	0.4176	3.2232	-37.5691	-50.8229
79	2.7085	2.4084	0.856	3.8354	-1.2417	2.6326	0.1706	0.2113	-0.6013	0.1257	-0.8438	0.0347	2.0689	0.927	0.4132	0.573	0.0778	-0.0365	0.3223	-0.0333	0.4154	4.6447	-34.3144	-42.1949
80	1.0705	-1.5525	-0.2536	2.7443	-0.4591	3.1138	-0.0743	0.2743	0.0624	-0.3295	-0.03	0.1754	-0.0405	0.0845	0.0616	0.3006	-0.0756	0.01	0.2486	-0.0535	0.3548	-3.0305	-27.6767	-51.6448
81	4.3441	-3.5419	-3.7787	4.7456	1.2386	5.0152	0.1993	0.5106	-0.5361	-0.3916	-0.1461	1.0801	0.2882	-0.8398	-0.0296	0.4043	-0.1359	-0.0529	0.3372	-0.0643	0.4281	-12.4398	-29.839	-55.0001
82	5.0082	2.2543	0.704	6.9276	1.7114	3.7165	0.0265	-0.1725	0.2565	0.2068	-0.1061	0.6571	0.1756	-0.5879	0.0233	0.1541	-0.059	0.0059	0.242	0.0003	0.29	-7.5284	-18.8277	-46.4285
83	5.9739	-5.4349	3.7155	4.9642	-3.4134	2.5345	-0.4787	-2.7465	0.9803	1.8256	0.4655	-0.8035	-0.2733	-1.0196	0.0682	0.9682	-0.0877	-0.0326	0.7847	-0.0703	0.5642	0.2658	-9.7659	-35.9488
84	0.4673	0.5706	-0.784	4.758	-0.9668	5.1591	0.1476	-0.0958	0.01	0.1352	-0.112	0.6408	0.0179	-0.4992	-0.023	0.177	-0.0378	0.018	0.2714	-0.0402	0.3634	-9.515	-17.5111	-47.2757
85	6.4662	2.4524	-0.0758	2.2089	-0.7279	8.8851	-0.1509	-0.4509	0.3325	0.3313	-0.2095	-0.2688	0.3742	0.7068	0.2402	0.2739	-0.0587	-0.0355	0.2709	0.0463	0.3141	2.357	-20.9365	-39.5427
86	4.3731	-5.3232	1.1972	8.788	0.5476	5.1814	-0.3792	-0.5808	-0.7219	1.0366	0.4247	0.9394	-0.0197	-0.707	-0.1885	0.4214	-0.0651	0.1986	0.3723	-0.0253	0.4335	-12.5528	-19.1846	-37.4907
87	2.498	-1.028	1.549	1.9563	0.901	2.551	0.7427	0.0081	0.2884	-0.6082	-0.1918	-0.3291	-0.5522	1.5522	-0.7766	0.2989	0.0313	0.0899	0.4046	-0.0393	0.3655	11.1308	-3.9339	-67.619
88	3.351	0.5597	-3.5007	8.2769	2.7293	4.9789	-0.3406	-0.4852	-0.8597	0.4248	0.0734	-0.3604	0.9058	0.983	0.3238	0.2215	0.0872	0.0027	0.3034	0.047	0.2721	3.9564	-8.0801	-52.716
89	9.151	0.5186	-5.3038	5.5898	-1.1129	3.2264	0.6164	-0.0668	0.3825	0.359	0.0595	-0.493	-0.286	0.3868	-0.7437	0.2045	-0.0058	0.011	0.2646	-0.0015	0.2697	8.4237	-3.5568	-56.5191
90	2.6295	-1.0952	0.5865	5.1689	3.5326	3.5287	-0.0538	-0.3315	0.7205	-0.9784	0.8536	-1.4954	-0.6261	1.1795	-0.6464	0.281	-0.0503	0.1421	0.4864	-0.1461	0.4811	15.6897	-9.0864	-69.3386
91	4.8905	-1.6981	2.9035	5.0637	2.816	5.6439	0.3188	-0.1177	-0.3285	-0.4012	0.3103	-0.9741	0.4184	0.7215	-0.5158	0.2229	0.1134	0.0445	0.3196	0.0008	0.3641	14.2618	-15.6804	-63.0283
92	5.567	-0.1508	-3.167	5.653	3.1419	4.7411	-0.3228	-0.1506	-0.9796	0.653	0.1131	-2.1962	0.6823	1.8718	0.3825	0.1841	0.0389	0.0064	0.4861	-0.0476	0.594	16.5643	-12.0739	-56.5314
93	3.9333	-1.3665	-2.5952	2.0081	-3.3497	8.1094	-0.4716	-0.8727	0.4862	1.838	0.649	-1.0668	-0.156	0.6784	0.0737	0.4392	-0.0629	-0.1737	0.424	-0.0105	0.5456	14.2591	-11.2082	-50.9411
94	2.4745	3.9474	-1.6842	7.0587	-0.2447	8.985	0.1988	-0.3397	-0.2894	0.2525	-0.1643	-0.7736	1.0506	1.0486	-0.096	0.2732	0.0842	0.008	0.3153	-0.0048	0.3564	8.677	-18.9298	-55.1986
95	5.2783	4.2446	-2.6797	4.8329	0.9349	9.0887	-0.1559	1.2758	0.238	-0.7506	0.2049	0.7478	1.0445	-0.4358	-0.1908	0.3209	-0.0189	0.001	0.3179	-0.0403	0.3335	0.7368	-14.8139	-66.109
96	4.6016	-1.8467	-4.5762	8.6841	5.1132	5.9177	-0.369	-0.2595	-1.1458	-0.3684	0.2723	-0.4569	1.1991	0.9583	0.0038	0.5484	0.1138	0.0815	0.4067	-0.0694	0.533	10.5271	-21.0222	-66.4183
97	2.2871	2.4899	-2.502	5.7752	-7.2808	9.5169	0.1479	0.3493	-0.6832	-1.2484	0.3121	-0.4564	0.3731	0.0571	-0.3397	0.4272	0.0017	0.052	0.4148	-0.1064	0.2943	8.5675	-15.9221	-71.3643
98	7.5432	-1.1201	-1.3075	4.5778	6.0311	7.9272	-0.1064	0.1925	0.4917	-0.8623	0.5525	0.1828	-0.7661	-0.271	-0.3793	0.3377	-0.0255	0.0483	0.3289	0.0117	0.2438	4.995	-5.7846	-68.2836
99	1.3548	0.898	-1.1756	4.3098	-5.2648	6.3035	0.0043	0.0548	-0.2281	0.1843	-0.1976	-0.3754	0.0431	0.2521	0.1714	0.231	0.0116	0.0006	0.2364	-0.025	0.2618	4.0346	-17.9444	-52.9285
100	4.2585	3.0981	3.5082	3.7994	-0.445	8.7661	0.5637	-0.6371	0.2612	-0.1976	-0.3576	-1.3862	-0.0576	0.4325	-0.1025	0.7399	-0.0546	-0.1436	0.5397	-0.0969	0.662	16.7892	-15.1254	-16.3718
101	7.2108	0.8515	-0.8211	9.2773	2.9849	4.8464	0.6376	-0.2888	-0.2403	1.494	-0.0854	-0.8735	-0.0771	-0.1534	-0.2969	0.5495	0.0559	-0.1732	0.3917	0.001	0.4351	8.3761	-13.4427	-6.7817
102	5.7323	0.394	-1.2455	7.2747	-1.6922	3.9974	0.0591	-0.4885	-0.5835	0.9895	-0.2451	-0.1151	1.0959	0.5645	0.0598	0.8946	0.0403	-0.0496	0.4313	-0.0534	0.3311	4.2231	-16.5032	-5.8824
103	8.6385	2.0131	-0.116	2.6241	2.3789	3.1135	0.6746	1.2103	-0.0587	-1.4098	0.6308	-0.5702	-1.4628	0.1175	-1.2854	0.563	-0.0066	0.0422	0.4604	-0.0251	0.4184	8.5454	-11.3956	-23.1
104	5.6576	6.0084	4.8994	6.4455	5.2674	4.3074	-0.105	0.2896	-0.5098	-0.8067	1.2157	-2.3705	-0.2687	1.7415	-1.1159	0.3931	-0.0638	0.0968	0.5848	-0.1356	0.5971	10.7941	-18.263	-26.2635
105	7.2556	-0.8648	2.705	3.2339	3.8399	7.3728	-0.4539	0.283	-0.3832	0.352	-0.1538	0.3795	1.3802	-0.7918	0.3396	0.6154	-0.1718	-0.0283	0.3358	-0.0404	0.3431	-3.6878	-27.5183	-25.6979
106	2.9085	2.8706	1.3881	4.6283	-0.3442	4.5816	0.3848	0.5797	-0.8133	0.4789	-0.1254	-0.4467	1.4345	0.6145	-0.2599	0.9611	0.0767	-0.0421	0.3342	-0.045	0.392	2.0792	-32.3366	-20.4335
107	5.5821	-1.1028	-5.1136	8.6238	3.6986	5.6699	-0.4093	0.0795	-1.2654	-0.0012	0.4683	0.2926	2.1677	-0.2065	0.0768	1.0236	-0.1139	-0.0467	0.355	-0.0882	0.6001	-0.0198	-38.0284	-25.0265
108	7.63	-2.8633	2.7594	1.9178	-2.2499	2.7785	-0.5876	0.279	-1.7028	1.0765	-0.211	0.2796	1.2958	-0.2795	0.7312	1.2093	-0.2507	0.0877	0.4502	0.0399	0.7889	-4.4851	-38.2588	-16.8034
109	1.3059	-0.8397	1.5076	4.3982	5.3293	1.9969	-0.1324	-0.5493	-0.6494	1.3464	-0.2517	0.5297	1.3285	-1.3772	-0.5173	1.913	-0.4455	0.0741	0.2497	0.3479	0.4461	-5.0758	-35.2633	-6.161
110	3.5787	-2.1974	0.4745	1.3655	-0.7172	7.8984	-0.6323	0.521	-0.4289	-0.0043	0.6114	0.3458	1.1555	-1.1372	-0.0546	1.5059	-0.2955	0.1056	0.7387	0.0897	0.4588	-7.4244	-25.9465	-1.1143
111	6.7724	-1.5999	-0.3568	0.404	0.0829	0.0186	-0.0093	-3.018	0.6298	1.2154	-0.0004	1.0029	-0.5964	-2.5511	0.0069	1.0897	-0.1039	0.2879	1.6025	-0.0883	0.644	-12.9855	-21.5052	-3.4209
112	1.9275	-1.217	-2.3466	0.819	1.6737	9.561	-0.3377	0.2412	-0.1135	-0.3814	0.2315	-0.0035	0.2993	-0.7989	0.1167	1.1187	-0.4577	0.1701	0.7511	0.0681	0.6448	-14.6799	-32.4797	-7.732
113	8.9743	4.6825	3.4159	3.4389	0.8549	2.19	0.5246	-0.9987	-1.6055	0.1058	-0.4095	-0.353	0.9782	-1.9319	-0.1924	0.914	-0.2953	0.1156	0.6789	-0.0394	0.5298	-12.3055	-29.0738	-19.1498
114	5.3886	-0.4277	-1.8014	2.3471	-3.3085	7.0325	0.1062	0.2319	-0.534	1.2153	-0.3991	-0.1575	1.6217	0.0684	0.1345	1.1197	-0.1307	-0.0084	0.403	-0.0141	0.4282	-0.2552	-29.1141	-8.076
115	3.6553	-0.9982	-0.4295	5.5583	0.5365	0.3658	-0.3172	-0.0257	0.0104	-0.02	0.3178	-0.0804	0.7177	-0.1822	0.0551	0.6214	-0.0391	-0.0153	0.332	-0.0436	0.263	3.7072	-19.4646	-16.712
116	6.7987	-4.4307	4.9929	3.2196	-3.7656	4.4619	-0.5141	3.5874	1.0132	-0.6737	-1.0691	0.6113	-0.938	1.5947	1.3628	0.7828	-0.0174	-0.0637	0.8214	0.0453	0.4737	2.2516	-9.8437	-26.4204
117	3.5228	-1.2344	-0.6921	8.9738	-5.2302	7.7619	0.0578	0.4397	-0.1257	0.6848	-0.1518	-0.5456	0.249	0.7869	-0.0011	0.5283	-0.0445	-0.1415	0.3745	-0.0749	0.3141	7.0535	-19.1231	-11.2991
118	8.382	-1.5648	3.0711	5.3913	-0.1214	7.0639	0.1586	0.1156	-0.2441	-0.0139	0.0086	-0.254	0.9339	-0.091	-0.1805	0.4327	-0.0524	0.0235	0.2469	-0.0401	0.2843	0.9783	-21.3078	-20.9061
119	4.4571	3.458	-5.3112	8.516	-2.126	9.2657	-0.8901	-0.7609	-2.1642	-0.2591	0.8969	-2.046	0.7144	2.1932	-0.3399	0.4403	0.0668	-0.0253	0.6759	-0.1953	0.6745	13.3302	-23.2721	-24.4901
120	6.1879	-5.4159	-5.9559	4.7997	5.2626	5.7975	0.288	-0.4658	1.1441	0.3333	0.4303	0.2581	-0.3459	-0.7785	-0.621	0.5879	-0.0311	0.1238	0.4904	-0.023	0.3855	-6.5775	2.5228	1.8553
121	4.0404	-1.1817	-2.9905	3.1075	-0.9501	4.1626	0.0388	0.6781	-0.3207	0.1166	0.0593	0.5791	1.0404	-0.1202	-0.1705	0.3578	-0.0604	0.0268	0.3611	0.0119	0.3219	-0.8906	-5.9146	-11.517
122	3.7274	-3.5878	-5.6553	7.4292	5.7288	8.8685	-0.0544	0.51	0.3312	-0.1768	0.4119	-0.0786	0.2443	-0.2662	-0.3696	0.4154	-0.0213	0.05	0.4033	0.0205	0.2567	-3.3015	0.8552	-7.5611
123	5.92	5.0372	-5.4795	4.338	-4.6793	5.0971	0.566	-0.3777	1.1411	1.1869	0.0779	1.5314	-1.2277	-0.8104	-0.5356	0.4642	0.0261	0.1152	0.4755	-0.0372	0.5182	-12.82	0.8926	4.7792
124	5.4452	-0.315	-4.0901	0.2832	1.2199	6.7269	0.0054	-0.0561	-0.1991	0.7297	-0.2156	0.5295	-0.2101	-0.9267	0.0128	0.5427	-0.1743	0.1159	0.5218	-0.0133	0.361	-14.6981	-5.8331	0.5957
125	4.1751	4.0031	-0.8884	4.0072	-0.5619	7.7753	-0.4086	0.4655	0.7515	-0.3401	0.2443	1.9266	0.3397	-1.5908	0.0431	0.3779	-0.0662	0.0146	0.6536	0.0009	0.4191	-13.5359	-2.9603	-7.9563
126	2.5082	2.0159	-0.9561	4.2688	-3.7178	3.7344	0.1311	1.3582	-0.0123	-0.6582	-0.3464	0.2867	-0.0788	-1.6713	0.1473	0.6869	0.002	-0.0279	0.8951	-0.0491	0.5205	-12.6464	-6.2344	-14.1164
127	3.0335	-1.1304	0.9525	2.5975	-3.0002	3.5412	0.4358	1.1547	0.0928	-0.0027	0.1713	0.3945	0.8447	-0.1516	-0.3181	0.6573	-0.1538	0.0238	0.4685	-0.0094	0.3746	-10.1836	-13.543	-8.9912
128	5.7902	-5.7677	-0.3005	5.7545	-0.3064	8.7327	-0.7309	-1.7209	0.0242	1.0113	0.67	-0.5414	1.8567	0.351	-0.0364	0.5083	0.0486	0.0013	0.4815	-0.0169	0.3177	-2.3703	-10.9038	2.4226
129	4.0384	-5.7345	-1.7666	8.085	2.4758	2.0812	0.5746	-0.0863	-0.9534	0.6783	-0.6332	1.4321	-0.1026	-0.3802	-0.152	0.7309	-0.2036	0.1714	0.5245	-0.0243	0.5501	-12.4504	-13.5971	2.8665
130	5.0948	-4.7216	-0.1711	6.1505	1.9585	1.9286	0.1817	-0.3191	-0.679	0.1153	-0.6424	0.5743	0.5435	0.271	0.5506	0.5517	0.1262	0.1423	0.6478	0.0458	0.4359	-9.9711	-8.6877	7.794
131	6.3622	-0.5378	-2.8026	2.2461	-3.6778	8.4919	-0.3195	-0.6428	0.3563	1.277	0.1836	0.1305	-0.029	0.6723	0.2639	0.5725	-0.0522	0.0726	0.3953	-0.0662	0.2781	-2.0146	-1.3856	4.5796
132	1.853	1.2971	1.1242	6.6488	6.5085	6.3674	0.0771	-0.033	0.1763	0.7108	-0.3887	1.0934	0.6834	-0.7391	0.3184	0.5378	-0.1487	0.0665	0.4554	0.0134	0.3289	-5.7536	-14.3271	-9.8873

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	(chain A and resid 2:8)
2	X-RAY DIFFRACTION	2	(chain A and resid 9:14)
3	X-RAY DIFFRACTION	3	(chain A and resid 15:21)
4	X-RAY DIFFRACTION	4	(chain A and resid 22:27)
5	X-RAY DIFFRACTION	5	(chain A and resid 28:33)
6	X-RAY DIFFRACTION	6	(chain A and resid 34:46)
7	X-RAY DIFFRACTION	7	(chain A and resid 47:55)
8	X-RAY DIFFRACTION	8	(chain A and resid 56:60)
9	X-RAY DIFFRACTION	9	(chain A and resid 61:67)
10	X-RAY DIFFRACTION	10	(chain A and resid 68:73)
11	X-RAY DIFFRACTION	11	(chain A and resid 74:79)
12	X-RAY DIFFRACTION	12	(chain A and resid 80:86)
13	X-RAY DIFFRACTION	13	(chain A and resid 87:92)
14	X-RAY DIFFRACTION	14	(chain A and resid 93:101)
15	X-RAY DIFFRACTION	15	(chain A and resid 102:110)
16	X-RAY DIFFRACTION	16	(chain A and resid 111:122)
17	X-RAY DIFFRACTION	17	(chain A and resid 123:130)
18	X-RAY DIFFRACTION	18	(chain A and resid 131:139)
19	X-RAY DIFFRACTION	19	(chain A and resid 140:154)
20	X-RAY DIFFRACTION	20	(chain A and resid 155:159)
21	X-RAY DIFFRACTION	21	(chain B and resid 80:86)
22	X-RAY DIFFRACTION	22	(chain B and resid 87:94)
23	X-RAY DIFFRACTION	23	(chain B and resid 95:99)
24	X-RAY DIFFRACTION	24	(chain B and resid 100:105)
25	X-RAY DIFFRACTION	25	(chain B and resid 106:110)
26	X-RAY DIFFRACTION	26	(chain B and resid 111:115)
27	X-RAY DIFFRACTION	27	(chain B and resid 116:120)
28	X-RAY DIFFRACTION	28	(chain B and resid 121:125)
29	X-RAY DIFFRACTION	29	(chain B and resid 126:130)
30	X-RAY DIFFRACTION	30	(chain B and resid 131:135)
31	X-RAY DIFFRACTION	31	(chain B and resid 136:140)
32	X-RAY DIFFRACTION	32	(chain B and resid 141:150)
33	X-RAY DIFFRACTION	33	(chain B and resid 151:156)
34	X-RAY DIFFRACTION	34	(chain C and resid 2:6)
35	X-RAY DIFFRACTION	35	(chain C and resid 7:13)
36	X-RAY DIFFRACTION	36	(chain C and resid 14:22)
37	X-RAY DIFFRACTION	37	(chain C and resid 23:29)
38	X-RAY DIFFRACTION	38	(chain C and resid 30:34)
39	X-RAY DIFFRACTION	39	(chain C and resid 35:50)
40	X-RAY DIFFRACTION	40	(chain C and resid 51:56)
41	X-RAY DIFFRACTION	41	(chain C and resid 57:61)
42	X-RAY DIFFRACTION	42	(chain C and resid 62:66)
43	X-RAY DIFFRACTION	43	(chain C and resid 67:77)
44	X-RAY DIFFRACTION	44	(chain C and resid 78:84)
45	X-RAY DIFFRACTION	45	(chain C and resid 85:89)
46	X-RAY DIFFRACTION	46	(chain C and resid 90:95)
47	X-RAY DIFFRACTION	47	(chain C and resid 96:100)
48	X-RAY DIFFRACTION	48	(chain C and resid 101:112)
49	X-RAY DIFFRACTION	49	(chain C and resid 113:120)
50	X-RAY DIFFRACTION	50	(chain C and resid 121:126)
51	X-RAY DIFFRACTION	51	(chain C and resid 127:132)
52	X-RAY DIFFRACTION	52	(chain C and resid 133:152)
53	X-RAY DIFFRACTION	53	(chain C and resid 153:158)
54	X-RAY DIFFRACTION	54	(chain D and resid 80:87)
55	X-RAY DIFFRACTION	55	(chain D and resid 88:93)
56	X-RAY DIFFRACTION	56	(chain D and resid 94:98)
57	X-RAY DIFFRACTION	57	(chain D and resid 99:103)
58	X-RAY DIFFRACTION	58	(chain D and resid 104:110)
59	X-RAY DIFFRACTION	59	(chain D and resid 111:115)
60	X-RAY DIFFRACTION	60	(chain D and resid 116:120)
61	X-RAY DIFFRACTION	61	(chain D and resid 121:124)
62	X-RAY DIFFRACTION	62	(chain D and resid 125:130)
63	X-RAY DIFFRACTION	63	(chain D and resid 131:135)
64	X-RAY DIFFRACTION	64	(chain D and resid 136:141)
65	X-RAY DIFFRACTION	65	(chain D and resid 142:151)
66	X-RAY DIFFRACTION	66	(chain D and resid 152:156)
67	X-RAY DIFFRACTION	67	(chain E and resid 2:6)
68	X-RAY DIFFRACTION	68	(chain E and resid 7:18)
69	X-RAY DIFFRACTION	69	(chain E and resid 19:23)
70	X-RAY DIFFRACTION	70	(chain E and resid 24:28)
71	X-RAY DIFFRACTION	71	(chain E and resid 29:33)
72	X-RAY DIFFRACTION	72	(chain E and resid 34:47)
73	X-RAY DIFFRACTION	73	(chain E and resid 48:52)
74	X-RAY DIFFRACTION	74	(chain E and resid 53:63)
75	X-RAY DIFFRACTION	75	(chain E and resid 64:76)
76	X-RAY DIFFRACTION	76	(chain E and resid 77:83)
77	X-RAY DIFFRACTION	77	(chain E and resid 84:88)
78	X-RAY DIFFRACTION	78	(chain E and resid 89:94)
79	X-RAY DIFFRACTION	79	(chain E and resid 95:99)
80	X-RAY DIFFRACTION	80	(chain E and resid 100:108)
81	X-RAY DIFFRACTION	81	(chain E and resid 109:113)
82	X-RAY DIFFRACTION	82	(chain E and resid 114:121)
83	X-RAY DIFFRACTION	83	(chain E and resid 122:126)
84	X-RAY DIFFRACTION	84	(chain E and resid 127:146)
85	X-RAY DIFFRACTION	85	(chain E and resid 147:152)
86	X-RAY DIFFRACTION	86	(chain E and resid 153:158)
87	X-RAY DIFFRACTION	87	(chain F and resid 80:85)
88	X-RAY DIFFRACTION	88	(chain F and resid 86:91)
89	X-RAY DIFFRACTION	89	(chain F and resid 92:96)
90	X-RAY DIFFRACTION	90	(chain F and resid 97:102)
91	X-RAY DIFFRACTION	91	(chain F and resid 103:108)
92	X-RAY DIFFRACTION	92	(chain F and resid 109:113)
93	X-RAY DIFFRACTION	93	(chain F and resid 114:118)
94	X-RAY DIFFRACTION	94	(chain F and resid 119:124)
95	X-RAY DIFFRACTION	95	(chain F and resid 125:130)
96	X-RAY DIFFRACTION	96	(chain F and resid 131:135)
97	X-RAY DIFFRACTION	97	(chain F and resid 136:140)
98	X-RAY DIFFRACTION	98	(chain F and resid 141:147)
99	X-RAY DIFFRACTION	99	(chain F and resid 148:156)
100	X-RAY DIFFRACTION	100	(chain G and resid 2:6)
101	X-RAY DIFFRACTION	101	(chain G and resid 7:12)
102	X-RAY DIFFRACTION	102	(chain G and resid 13:20)
103	X-RAY DIFFRACTION	103	(chain G and resid 21:26)
104	X-RAY DIFFRACTION	104	(chain G and resid 27:31)
105	X-RAY DIFFRACTION	105	(chain G and resid 32:41)
106	X-RAY DIFFRACTION	106	(chain G and resid 42:50)
107	X-RAY DIFFRACTION	107	(chain G and resid 51:58)
108	X-RAY DIFFRACTION	108	(chain G and resid 59:63)
109	X-RAY DIFFRACTION	109	(chain G and resid 64:72)
110	X-RAY DIFFRACTION	110	(chain G and resid 73:77)
111	X-RAY DIFFRACTION	111	(chain G and resid 78:84)
112	X-RAY DIFFRACTION	112	(chain G and resid 85:94)
113	X-RAY DIFFRACTION	113	(chain G and resid 95:101)
114	X-RAY DIFFRACTION	114	(chain G and resid 102:113)
115	X-RAY DIFFRACTION	115	(chain G and resid 114:122)
116	X-RAY DIFFRACTION	116	(chain G and resid 123:127)
117	X-RAY DIFFRACTION	117	(chain G and resid 128:138)
118	X-RAY DIFFRACTION	118	(chain G and resid 139:154)
119	X-RAY DIFFRACTION	119	(chain G and resid 155:159)
120	X-RAY DIFFRACTION	120	(chain H and resid 80:86)
121	X-RAY DIFFRACTION	121	(chain H and resid 87:91)
122	X-RAY DIFFRACTION	122	(chain H and resid 92:96)
123	X-RAY DIFFRACTION	123	(chain H and resid 97:101)
124	X-RAY DIFFRACTION	124	(chain H and resid 102:107)
125	X-RAY DIFFRACTION	125	(chain H and resid 108:114)
126	X-RAY DIFFRACTION	126	(chain H and resid 115:119)
127	X-RAY DIFFRACTION	127	(chain H and resid 120:123)
128	X-RAY DIFFRACTION	128	(chain H and resid 124:129)
129	X-RAY DIFFRACTION	129	(chain H and resid 130:135)
130	X-RAY DIFFRACTION	130	(chain H and resid 136:140)
131	X-RAY DIFFRACTION	131	(chain H and resid 141:147)
132	X-RAY DIFFRACTION	132	(chain H and resid 148:156)