+Open data
-Basic information
Entry | Database: PDB / ID: 6n4a | ||||||
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Title | PII-like SbtB from Cyanobium sp PCC 7001 (apo) | ||||||
Components | PII-like SbtB | ||||||
Keywords | SIGNALING PROTEIN / Pii-like signalling protein / SbtB / cyanobacteria | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Cyanobium sp. PCC 7001 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.3 Å | ||||||
Authors | Kaczmarski, J.A. / Jackson, C.J. | ||||||
Citation | Journal: Biorxiv / Year: 2019 Title: Structure and function of SbtB from Cyanobium sp. 7001 Authors: Jackson, C. / Kaczmarski, J.A. / Price, D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6n4a.cif.gz | 108.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6n4a.ent.gz | 84.3 KB | Display | PDB format |
PDBx/mmJSON format | 6n4a.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6n4a_validation.pdf.gz | 442.5 KB | Display | wwPDB validaton report |
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Full document | 6n4a_full_validation.pdf.gz | 450.3 KB | Display | |
Data in XML | 6n4a_validation.xml.gz | 12.3 KB | Display | |
Data in CIF | 6n4a_validation.cif.gz | 18.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/n4/6n4a ftp://data.pdbj.org/pub/pdb/validation_reports/n4/6n4a | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 11396.966 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Cyanobium sp. PCC 7001 (bacteria) / Gene: CPCC7001_1671 / Production host: Escherichia coli (E. coli) / References: UniProt: B5II98 #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.14 Å3/Da / Density % sol: 42.49 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion / Details: 25 mM Na2H/KH2PO4 20% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: Australian Synchrotron / Beamline: MX1 / Wavelength: 0.953657 Å |
Detector | Type: ADSC QUANTUM 210r / Detector: CCD / Date: Jun 12, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.953657 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→49.03 Å / Num. obs: 24704 / % possible obs: 98 % / Redundancy: 3.8 % / Net I/σ(I): 15.2 |
Reflection shell | Resolution: 2.3→2.38 Å / Redundancy: 3.8 % / Num. unique obs: 2386 / CC1/2: 0.574 / % possible all: 95.2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.3→49.03 Å / Cross valid method: THROUGHOUT /
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Displacement parameters | Biso max: 141.6 Å2 / Biso mean: 75.1516 Å2 / Biso min: 32.46 Å2 | ||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.3→49.03 Å
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