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- PDB-6mn5: Crystal structure of aminoglycoside acetyltransferase AAC(3)-IVa,... -

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Basic information

Entry
Database: PDB / ID: 6mn5
TitleCrystal structure of aminoglycoside acetyltransferase AAC(3)-IVa, H154A mutant, in complex with gentamicin C1A
ComponentsAminoglycoside N(3)-acetyltransferase, AAC(3)-IVa
KeywordsTRANSFERASE/ANTIBIOTIC / ANTIBIOTIC_NAT FAMILY / ACETYLTRANSFERASE / AMINOGLYCOSIDE / ANTIBIOTIC RESISTANCE / COENZYME A / GENTAMICIN / AAC(3)-IVA / CSGID / TRANSFERASE / CENTER FOR STRUCTURAL GENOMICS OF INFECTIOUS DISEASES / National Institute of Allergy and Infectious Diseases / NIAID / TRANSFERASE-ANTIBIOTIC complex
Function / homology
Function and homology information


aminoglycoside 3-N-acetyltransferase activity / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups / response to antibiotic / metal ion binding
Similarity search - Function
Aminoglycoside N(3)-acetyltransferase / Aminoglycoside 3-N-acetyltransferase / Aminoglycoside 3-N-acetyltransferase-like
Similarity search - Domain/homology
Chem-LLL / Chem-PE3 / Aminoglycoside N(3)-acetyltransferase
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.58 Å
AuthorsStogios, P.J. / Evdokimova, E. / Kim, Y. / Di Leo, R. / Savchenko, A. / Joachimiak, A. / Satchell, K.J. / Center for Structural Genomics of Infectious Diseases (CSGID)
Funding support United States, 2items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)HHSN272201700060C United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)HHSN272201200026C United States
CitationJournal: Commun Biol / Year: 2022
Title: Structural and molecular rationale for the diversification of resistance mediated by the Antibiotic_NAT family.
Authors: Stogios, P.J. / Bordeleau, E. / Xu, Z. / Skarina, T. / Evdokimova, E. / Chou, S. / Diorio-Toth, L. / D'Souza, A.W. / Patel, S. / Dantas, G. / Wright, G.D. / Savchenko, A.
History
DepositionOct 1, 2018Deposition site: RCSB / Processing site: RCSB
Revision 1.0Oct 24, 2018Provider: repository / Type: Initial release
Revision 1.1Dec 18, 2019Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.2Apr 6, 2022Group: Database references / Derived calculations / Structure summary
Category: chem_comp / citation ...chem_comp / citation / citation_author / database_2 / entity / pdbx_entity_nonpoly
Item: _chem_comp.name / _citation.country ..._chem_comp.name / _citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _entity.pdbx_description / _pdbx_entity_nonpoly.name
Revision 1.3Oct 11, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Aminoglycoside N(3)-acetyltransferase, AAC(3)-IVa
B: Aminoglycoside N(3)-acetyltransferase, AAC(3)-IVa
C: Aminoglycoside N(3)-acetyltransferase, AAC(3)-IVa
D: Aminoglycoside N(3)-acetyltransferase, AAC(3)-IVa
E: Aminoglycoside N(3)-acetyltransferase, AAC(3)-IVa
F: Aminoglycoside N(3)-acetyltransferase, AAC(3)-IVa
hetero molecules


Theoretical massNumber of molelcules
Total (without water)180,75779
Polymers169,8986
Non-polymers10,85973
Water9,170509
1
A: Aminoglycoside N(3)-acetyltransferase, AAC(3)-IVa
hetero molecules


Theoretical massNumber of molelcules
Total (without water)30,08317
Polymers28,3161
Non-polymers1,76616
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
2
B: Aminoglycoside N(3)-acetyltransferase, AAC(3)-IVa
hetero molecules


Theoretical massNumber of molelcules
Total (without water)30,53115
Polymers28,3161
Non-polymers2,21514
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
3
C: Aminoglycoside N(3)-acetyltransferase, AAC(3)-IVa
hetero molecules


Theoretical massNumber of molelcules
Total (without water)30,59316
Polymers28,3161
Non-polymers2,27715
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
4
D: Aminoglycoside N(3)-acetyltransferase, AAC(3)-IVa
hetero molecules


Theoretical massNumber of molelcules
Total (without water)30,62316
Polymers28,3161
Non-polymers2,30715
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
5
E: Aminoglycoside N(3)-acetyltransferase, AAC(3)-IVa
hetero molecules


Theoretical massNumber of molelcules
Total (without water)29,5569
Polymers28,3161
Non-polymers1,2408
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
6
F: Aminoglycoside N(3)-acetyltransferase, AAC(3)-IVa
hetero molecules


Theoretical massNumber of molelcules
Total (without water)29,3706
Polymers28,3161
Non-polymers1,0545
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
Unit cell
Length a, b, c (Å)77.609, 131.881, 266.872
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

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Protein , 1 types, 6 molecules ABCDEF

#1: Protein
Aminoglycoside N(3)-acetyltransferase, AAC(3)-IVa


Mass: 28316.393 Da / Num. of mol.: 6 / Mutation: H154A
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli)
Gene: aacC4, aac(3), aac(3)-IV, aac(3)IV, aac3-VI, aac3-VI_1, aac3-VI_2, B9T59_28975, BEN53_26235, BJJ90_27515, BK373_27930, BK400_28640, BVL39_27730, BZL31_07055, BZL69_29365, C5P01_27985, C7235_ ...Gene: aacC4, aac(3), aac(3)-IV, aac(3)IV, aac3-VI, aac3-VI_1, aac3-VI_2, B9T59_28975, BEN53_26235, BJJ90_27515, BK373_27930, BK400_28640, BVL39_27730, BZL31_07055, BZL69_29365, C5P01_27985, C7235_00480, CR538_26885, CWS33_26775, CXB56_01995, pEC012_00026, SAMEA3472055_02859, SAMEA3472064_03809, SAMEA3472078_05206, SAMEA3472108_05065, SAMEA3472117_05674, SAMEA3472119_04576, SAMEA3472121_05663, SAMEA3485115_04534, SAMEA3751392_04817, SAMEA3752267_04833, SAMEA3752372_05888, SAMEA3752378_04585, SAMEA3752509_02517, SAMEA3752558_04925, SAMEA3752685_02521, SAMEA3753064_04967, SAMEA3753137_02787, SAMEA3753290_03667, SAMEA3753300_05389, SAMEA3753355_02348
Plasmid: pET19bTEV / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)Gold
References: UniProt: Q306W4, aminoglycoside 3-N-acetyltransferase

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Non-polymers , 7 types, 582 molecules

#2: Chemical
ChemComp-LLL / (2R,3R,4R,5R)-2-((1S,2S,3R,4S,6R)-4,6-DIAMINO-3-((2R,3R,6S)-3-AMINO-6-(AMINOMETHYL)-TETRAHYDRO-2H-PYRAN-2-YLOXY)-2-HYDR OXYCYCLOHEXYLOXY)-5-METHYL-4-(METHYLAMINO)-TETRAHYDRO-2H-PYRAN-3,5-DIOL / GENTAMICIN C1A


Mass: 449.542 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: C19H39N5O7
#3: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: Zn
#4: Chemical
ChemComp-EPE / 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / HEPES


Mass: 238.305 Da / Num. of mol.: 12 / Source method: obtained synthetically / Formula: C8H18N2O4S / Comment: pH buffer*YM
#5: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C3H8O3
#6: Chemical...
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 41 / Source method: obtained synthetically / Formula: C2H6O2
#7: Chemical ChemComp-PE3 / 3,6,9,12,15,18,21,24,27,30,33,36,39-TRIDECAOXAHENTETRACONTANE-1,41-DIOL / POLYETHYLENE GLYCOL


Mass: 634.751 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C28H58O15
#8: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 509 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 4.05 Å3/Da / Density % sol: 69.61 %
Crystal growTemperature: 298 K / Method: vapor diffusion, sitting drop / pH: 7.5 / Details: 0.1 M HEPES pH 7, 30% PEG 1k, 1 mM gentamicin

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.97929 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Mar 1, 2018
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97929 Å / Relative weight: 1
ReflectionResolution: 2.58→40 Å / Num. obs: 83508 / % possible obs: 95.7 % / Redundancy: 3.7 % / Rmerge(I) obs: 0.086 / Rpim(I) all: 0.048 / Net I/σ(I): 14.08
Reflection shellResolution: 2.58→2.62 Å / Redundancy: 2.5 % / Rmerge(I) obs: 0.542 / Mean I/σ(I) obs: 1.08 / Num. unique all: 3527 / CC1/2: 0.703 / Rpim(I) all: 0.371 / % possible all: 82

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Processing

Software
NameVersionClassification
PHENIX(dev_3026: ???)refinement
HKL-3000data reduction
HKL-3000data scaling
PHENIXphasing
PHENIXmodel building
Cootmodel building
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 3SMA

3sma


Resolution: 2.58→38.401 Å / SU ML: 0.28 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 23.72
RfactorNum. reflection% reflectionSelection details
Rfree0.2214 2000 2.4 %RANDOM
Rwork0.1866 ---
obs0.1874 83420 95.61 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Refinement stepCycle: LAST / Resolution: 2.58→38.401 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms11813 0 608 509 12930
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00612697
X-RAY DIFFRACTIONf_angle_d0.87917239
X-RAY DIFFRACTIONf_dihedral_angle_d22.294849
X-RAY DIFFRACTIONf_chiral_restr0.0441922
X-RAY DIFFRACTIONf_plane_restr0.0052206
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.5786-2.6430.28121230.26815014X-RAY DIFFRACTION83
2.643-2.71450.29261350.2585496X-RAY DIFFRACTION93
2.7145-2.79430.31291430.25535801X-RAY DIFFRACTION96
2.7943-2.88450.27891430.24495828X-RAY DIFFRACTION97
2.8845-2.98750.26331440.24955885X-RAY DIFFRACTION97
2.9875-3.10710.28011450.22855924X-RAY DIFFRACTION99
3.1071-3.24850.25791470.21715962X-RAY DIFFRACTION99
3.2485-3.41960.25451450.20775917X-RAY DIFFRACTION98
3.4196-3.63370.20491440.19065846X-RAY DIFFRACTION97
3.6337-3.9140.19451470.16526008X-RAY DIFFRACTION98
3.914-4.30740.18071470.15055949X-RAY DIFFRACTION98
4.3074-4.92960.19491430.14855845X-RAY DIFFRACTION96
4.9296-6.20660.22471480.17835985X-RAY DIFFRACTION97
6.2066-38.40490.2081460.18055960X-RAY DIFFRACTION92
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.89163.9432-4.34433.9223-4.35124.69720.1701-0.67630.1760.55410.15620.26860.0766-1.0248-0.38540.4419-0.05070.01280.6719-0.04050.433727.26128.24826.8004
27.5337-0.7592-3.65786.4857-1.92358.85160.24540.0634-0.10240.2675-0.16040.30850.0005-0.5196-0.14320.2069-0.0417-0.04780.44060.00930.299628.860825.41216.908
32.6647-0.4058-0.14032.86250.89232.34250.20460.4206-0.2545-0.2022-0.1568-0.03850.1172-0.4055-0.02040.2939-0.0329-0.00150.6751-0.03950.410627.621816.47954.0604
48.858-0.7509-0.80654.25295.40236.86530.09331.9744-0.4678-1.2103-0.0062-0.37060.06390.285-0.16230.5044-0.030.0890.7793-0.03060.491136.939520.8487-1.4933
57.8521-2.45560.54943.971-1.04476.35440.16710.428-0.1045-0.0886-0.16-0.7113-0.06330.7401-0.00150.2404-0.05140.01230.4605-0.09840.462242.915519.72728.798
64.68633.09066.53766.50453.79319.11210.210.6378-0.955-0.2058-0.1234-0.1690.0991-0.0311-0.12710.6284-0.01910.10850.7835-0.11250.751541.85065.0038-2.1002
78.92834.21166.88093.78092.81862.08470.2761.0718-0.516-0.5226-0.14210.1574-0.0511-0.0855-0.0550.71210.04590.0290.847-0.27770.730238.04824.1063-2.5632
85.4966-4.5133-2.05415.82042.75882.38840.0306-0.2576-0.09470.38450.0235-0.4970.1810.278-0.04430.333-0.0068-0.07730.45590.03460.473349.037313.013215.9366
95.1691-1.3351-0.29163.180.82096.09550.09780.64440.1248-0.36740.0953-0.1239-0.20620.747-0.16940.3402-0.0642-0.08090.6276-0.04940.42056.818320.8858-5.8845
106.7575-2.27973.48912.8637-2.00344.44470.0267-0.0017-0.3277-0.09850.0996-0.04930.02610.2046-0.12290.2925-0.0423-0.00360.4291-0.03990.343710.97115.61310.7479
116.86950.76563.12318.143-4.22744.08760.6462-1.4058-0.42450.9841-0.23480.97390.0762-0.9019-0.57120.4992-0.03240.05860.82310.03590.60271.331914.414818.4511
123.77892.93561.43674.16580.01515.73420.234-0.3675-0.25640.06890.12940.41060.2335-0.1976-0.29320.28450.0086-0.05760.50920.02530.546-3.934710.7358.454
137.7505-1.30295.29862.587-4.10068.0716-0.023-0.1927-0.50.11740.3687-0.1650.12990.0646-0.58440.5737-0.0235-0.1380.58240.08650.79072.4184-1.851120.2415
143.3867-0.63043.64640.93740.33286.04420.0895-0.3792-0.80240.5264-0.415-0.42670.08220.85230.50230.8071-0.1832-0.17440.86960.34170.9976.3397-1.454620.524
154.40671.4539-2.52918.1293-6.25415.6021-0.04850.3956-0.2812-0.85530.39681.16840.3601-0.4017-0.27080.5094-0.0575-0.17420.6049-0.12930.5445-7.37647.9509-3.9954
164.18151.1856-0.43866.6003-2.71454.20220.14520.2869-1.30320.04930.0312-0.08720.7706-0.002-0.16750.4519-0.0241-0.15970.5226-0.12030.8192-7.5405-1.5065.1951
173.9421-1.6152.5959.4040.77392.532-0.03-0.59390.01750.52890.5010.1614-0.1418-0.0642-0.53050.4053-0.02750.07060.6549-0.0870.37161.183318.0082-18.013
181.6450.7823-1.38539.4234-4.56577.1246-0.1793-0.0144-0.0997-0.06150.3548-0.25020.2957-0.1104-0.14830.4007-0.0673-0.03580.6086-0.1320.41983.31617.5728-28.1102
194.4607-0.81022.13347.7602-0.61064.5477-0.14220.0657-0.30050.28440.177-0.78870.43910.8892-0.0580.4512-0.05630.09620.6132-0.06820.38510.59210.3823-41.2472
203.8871-0.4142-0.65996.85251.44044.83150.03860.5890.1008-0.74420.2164-0.4947-0.19170.3637-0.22260.5102-0.04650.0430.5423-0.0550.36047.545815.9484-44.316
219.32521.63140.52244.07280.25922.73860.24970.24250.4665-0.3755-0.0105-0.5987-0.48720.9315-0.14980.4709-0.07380.14360.6269-0.07880.462214.651925.574-38.0757
222.74073.73661.24436.95231.1930.792-0.23030.4051-0.3136-0.69220.8481-0.7582-0.24930.7537-0.62340.71590.05060.19341.1508-0.24950.764324.341713.6564-49.6684
237.15033.6093-6.68646.5442-4.65839.2090.4526-0.56170.70870.40630.0569-0.5205-0.78291.0007-0.4550.4988-0.10420.0350.7953-0.16640.596617.801930.0571-29.3212
247.0202-0.1774-3.31952.2179-0.90147.8545-0.8656-0.3428-0.0146-0.15211.1266-1.0980.00241.8754-0.32260.6697-0.13860.09361.3178-0.27671.124735.486523.1663-37.2566
258.3083-4.7573-5.33224.14565.00346.14850.530.27420.359-0.3367-0.068-0.72790.16860.1728-0.41250.51970.0166-0.03680.59160.05250.377634.766826.034535.0156
263.1034-1.24290.49644.3893-2.92328.6422-0.1366-0.1287-0.44280.5340.35910.40890.0364-0.3794-0.17980.41190.00410.05540.54770.04620.35629.262320.516652.8729
273.57682.1431-4.73175.9809-4.58156.9557-0.4624-1.23110.29381.25820.85450.8746-0.7731-0.8417-0.31771.08980.40570.17541.11580.11370.695922.872426.639463.3881
287.05992.20355.42218.8916-2.58026.5144-0.479-1.16410.42261.33160.33130.4269-0.9458-1.360.15880.65960.220.17570.75530.07310.669321.497532.293854.6899
297.1556-2.5484-1.1383.13980.34360.2170.08520.332-0.1741.04870.59570.5012-0.3699-1.8724-0.58830.74610.13160.20721.09640.27110.843211.246321.675858.2083
306.0726-6.63375.97647.2693-6.53675.9574-0.1536-1.0872-0.0808-0.57320.1976-0.22370.9353-0.2165-0.17381.11330.04990.49441.41760.39761.295311.918913.993364.9217
318.9093-0.9093-4.13624.80151.27455.86580.07380.00920.42290.18480.0980.9612-0.3426-1.2196-0.1820.570.18170.070.94640.15980.602213.262132.28144.0248
323.498-3.24943.5537.5443-0.97874.9412-0.08531.1535-0.11680.21121.09021.27760.5296-1.7846-0.98080.6619-0.1350.09411.65760.44931.355-1.454218.75450.889
334.463-1.7608-4.22388.3288-0.41984.5040.347-0.04230.56940.7214-0.8942-1.0534-1.56481.55440.7360.8622-0.1559-0.14231.13140.40810.834447.66211.003972.4506
342.8946-2.07860.2414.4824-4.9447.84680.09670.467-0.0731-1.2944-0.9645-0.38361.86051.30910.31141.11610.20970.26110.80180.25910.571843.01424.997752.1118
351.7433-0.1378-1.99460.01430.15212.3157-0.164-0.5372-1.9421-1.1766-0.5375-0.78472.0532.53930.77661.89021.13920.5511.50170.30931.184952.7587-2.385652.4346
360.3195-0.0952-0.96533.3741-1.10922.2661-0.26210.3867-0.546-1.207-0.28530.14122.70440.7090.40332.61870.54020.30331.18340.20180.965144.8861-10.672754.3533
373.791.34381.05068.63674.11111.9642-0.7397-0.4168-0.18040.4045-0.7321-0.3330.8191-0.2825-0.30473.6193-0.3060.00270.28880.64881.70636.6706-23.601456.0957
385.62290.9075-0.49666.31962.22718.554-0.42090.15320.25680.5788-1.10241.06141.303-2.74170.95250.9294-0.19340.17651.251-0.51150.7032-8.1828-3.2588-46.9516
393.98371.38421.43727.26980.66688.46410.6964-0.5512-0.82631.7906-0.6379-0.08683.3285-1.0624-0.03611.8159-0.1772-0.02020.8323-0.08350.60050.9965-6.2277-28.9493
402.3767-0.71130.33860.3363-0.32180.4231-0.2938-1.4204-2.1221.9163-0.18320.18752.2337-1.54920.7462.6591-0.84830.26021.40140.27781.2642-8.0751-15.8554-32.6529
41-0.1008-0.14760.14660.4898-0.21760.00140.5458-0.4541-0.56650.7468-0.3258-0.73713.24440.1962-0.20173.44390.1971-0.33071.06830.00551.21198.7642-18.1059-26.9253
423.27691.94820.81717.18960.27890.89020.4848-0.1928-1.20521.2874-0.55540.04083.1365-0.3678-0.02022.8983-0.35070.09321.1386-0.23131.0189-4.1056-23.0476-42.5376
435.2559-3.8767-1.83434.1847-1.54166.7469-0.9333-0.269-0.91032.42610.5013-0.76423.38030.75220.68943.60560.743-0.40911.3742-0.43142.163712.3791-31.3087-37.6657
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid -1 through 17 )
2X-RAY DIFFRACTION2chain 'A' and (resid 18 through 52 )
3X-RAY DIFFRACTION3chain 'A' and (resid 53 through 103 )
4X-RAY DIFFRACTION4chain 'A' and (resid 104 through 128 )
5X-RAY DIFFRACTION5chain 'A' and (resid 129 through 159 )
6X-RAY DIFFRACTION6chain 'A' and (resid 160 through 174 )
7X-RAY DIFFRACTION7chain 'A' and (resid 175 through 190 )
8X-RAY DIFFRACTION8chain 'A' and (resid 191 through 257 )
9X-RAY DIFFRACTION9chain 'B' and (resid -1 through 39 )
10X-RAY DIFFRACTION10chain 'B' and (resid 40 through 103 )
11X-RAY DIFFRACTION11chain 'B' and (resid 104 through 128 )
12X-RAY DIFFRACTION12chain 'B' and (resid 129 through 159 )
13X-RAY DIFFRACTION13chain 'B' and (resid 160 through 174 )
14X-RAY DIFFRACTION14chain 'B' and (resid 175 through 190 )
15X-RAY DIFFRACTION15chain 'B' and (resid 191 through 213 )
16X-RAY DIFFRACTION16chain 'B' and (resid 214 through 257 )
17X-RAY DIFFRACTION17chain 'C' and (resid -1 through 17 )
18X-RAY DIFFRACTION18chain 'C' and (resid 18 through 52 )
19X-RAY DIFFRACTION19chain 'C' and (resid 53 through 83 )
20X-RAY DIFFRACTION20chain 'C' and (resid 84 through 128 )
21X-RAY DIFFRACTION21chain 'C' and (resid 129 through 159 )
22X-RAY DIFFRACTION22chain 'C' and (resid 160 through 190 )
23X-RAY DIFFRACTION23chain 'C' and (resid 191 through 232 )
24X-RAY DIFFRACTION24chain 'C' and (resid 233 through 257 )
25X-RAY DIFFRACTION25chain 'D' and (resid -1 through 17 )
26X-RAY DIFFRACTION26chain 'D' and (resid 18 through 103 )
27X-RAY DIFFRACTION27chain 'D' and (resid 104 through 128 )
28X-RAY DIFFRACTION28chain 'D' and (resid 129 through 143 )
29X-RAY DIFFRACTION29chain 'D' and (resid 144 through 174 )
30X-RAY DIFFRACTION30chain 'D' and (resid 175 through 190 )
31X-RAY DIFFRACTION31chain 'D' and (resid 191 through 234 )
32X-RAY DIFFRACTION32chain 'D' and (resid 235 through 258 )
33X-RAY DIFFRACTION33chain 'E' and (resid -1 through 17 )
34X-RAY DIFFRACTION34chain 'E' and (resid 18 through 128 )
35X-RAY DIFFRACTION35chain 'E' and (resid 129 through 143 )
36X-RAY DIFFRACTION36chain 'E' and (resid 144 through 234 )
37X-RAY DIFFRACTION37chain 'E' and (resid 235 through 257 )
38X-RAY DIFFRACTION38chain 'F' and (resid -1 through 52 )
39X-RAY DIFFRACTION39chain 'F' and (resid 53 through 128 )
40X-RAY DIFFRACTION40chain 'F' and (resid 129 through 143 )
41X-RAY DIFFRACTION41chain 'F' and (resid 144 through 185 )
42X-RAY DIFFRACTION42chain 'F' and (resid 186 through 232 )
43X-RAY DIFFRACTION43chain 'F' and (resid 233 through 257 )

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