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Open data
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Basic information
Entry | Database: PDB / ID: 6mc2 | ||||||||||||||||||||||||||||
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Title | Crystal structure of a tetrameric DNA fold-back quadruplex | ||||||||||||||||||||||||||||
![]() | DNA (5'-D(*![]() DNA / quadruplex / fold-back / hexad / non-canonical | Function / homology | DNA | ![]() Biological species | synthetic construct (others) | Method | ![]() ![]() ![]() ![]() Chu, B. / Paukstelis, P.J. | Funding support | | ![]()
![]() ![]() Title: Crystal Structure of a Tetrameric DNA Fold-Back Quadruplex. Authors: Chu, B. / Zhang, D. / Hwang, W. / Paukstelis, P.J. History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 42.9 KB | Display | ![]() |
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PDB format | ![]() | 30.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 383.6 KB | Display | ![]() |
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Full document | ![]() | 385.6 KB | Display | |
Data in XML | ![]() | 5.3 KB | Display | |
Data in CIF | ![]() | 7.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: DNA chain | Mass: 2780.836 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #2: Chemical | ChemComp-MG / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.93 Å3/Da / Density % sol: 36.39 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, sitting drop / pH: 6.4 Details: 100 mM magnesium chloride, 6 mM hexamminecobalt(III) chloride, 30 mM sodium cacodylate, equilibrated against 20% MPD |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Oct 23, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.919 Å / Relative weight: 1 |
Reflection | Resolution: 1.05→23.48 Å / Num. obs: 19770 / % possible obs: 99.85 % / Redundancy: 3.5 % / Rmerge(I) obs: 0.07 / Rpim(I) all: 0.043 / Net I/σ(I): 9.2 |
Reflection shell | Resolution: 1.05→1.09 Å / Rmerge(I) obs: 0.228 / Num. unique obs: 1947 / Rpim(I) all: 0.16 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 10.177 Å2
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Refinement step | Cycle: 1 / Resolution: 1.05→23.48 Å
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Refine LS restraints |
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