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Open data
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Basic information
Entry | Database: PDB / ID: 6n4g | |||||||||||||||||||||||||||||||
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Title | Crystal structure of a tetrameric DNA fold-back quadruplex | |||||||||||||||||||||||||||||||
![]() | DNA (5'-D(*![]() DNA / quadruplex / fold-back / hexad / non-canonical | Function / homology | : / DNA | ![]() Biological species | synthetic construct (others) | Method | ![]() ![]() ![]() ![]() Chu, B. / Paukstelis, P.J. | Funding support | | ![]()
![]() ![]() Title: Crystal Structure of a Tetrameric DNA Fold-Back Quadruplex. Authors: Chu, B. / Zhang, D. / Hwang, W. / Paukstelis, P.J. History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 39.8 KB | Display | ![]() |
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PDB format | ![]() | 28.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 377.8 KB | Display | ![]() |
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Full document | ![]() | 377.8 KB | Display | |
Data in XML | ![]() | 4.8 KB | Display | |
Data in CIF | ![]() | 6.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: DNA chain | Mass: 2771.822 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #2: Chemical | ChemComp-BA / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.13 Å3/Da / Density % sol: 42.39 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, sitting drop / pH: 6.4 Details: 15% MPD, 120 mM barium chloride, 30 mM sodium cacodylate, equilibrated against 20% MPD |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Apr 23, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.92 Å / Relative weight: 1 |
Reflection | Resolution: 1.4→28.41 Å / Num. obs: 9187 / % possible obs: 99.5 % / Redundancy: 3.3 % / Rmerge(I) obs: 0.08 / Rpim(I) all: 0.052 / Net I/σ(I): 8.1 |
Reflection shell | Resolution: 1.4→1.42 Å / Rmerge(I) obs: 0.336 / Num. unique obs: 452 / Rpim(I) all: 0.224 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 18.634 Å2
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Refinement step | Cycle: 1 / Resolution: 1.4→28.41 Å
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