+Open data
-Basic information
Entry | Database: PDB / ID: 5in7 | |||||||||
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Title | X-RAY STRUCTURE OF THE N-TERMINAL DOMAIN OF HUMAN DOUBLECORTIN | |||||||||
Components | Neuronal migration protein doublecortinDevelopment of the nervous system | |||||||||
Keywords | TRANSFERASE / DCX DOMAIN / UBIQUITIN-LIKE FOLD / MICROTUBULE ASSOCIATED / SIGNALING PROTEIN | |||||||||
Function / homology | Function and homology information axoneme assembly / Neurofascin interactions / microtubule associated complex / central nervous system development / neuron migration / retina development in camera-type eye / nervous system development / microtubule binding / microtubule / cytoskeleton ...axoneme assembly / Neurofascin interactions / microtubule associated complex / central nervous system development / neuron migration / retina development in camera-type eye / nervous system development / microtubule binding / microtubule / cytoskeleton / neuron projection / intracellular signal transduction / protein kinase binding / cytosol Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.48 Å | |||||||||
Authors | Ruf, A. / Benz, J. / Burger, D. / D'Arcy, B. / Debulpaep, M. / Di Lello, P. / Fry, D. / Huber, W. / Kremer, T. / Laeremans, T. ...Ruf, A. / Benz, J. / Burger, D. / D'Arcy, B. / Debulpaep, M. / Di Lello, P. / Fry, D. / Huber, W. / Kremer, T. / Laeremans, T. / Matile, H. / Ross, A. / Rudolph, M.G. / Rufer, A.C. / Sharma, A. / Steinmetz, M.O. / Steyaert, J. / Schoch, G. / Stihle, M. / Thoma, R. | |||||||||
Funding support | Switzerland, 2items
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Citation | Journal: J.Biol.Chem. / Year: 2016 Title: Crystal Structures of the Human Doublecortin C- and N-terminal Domains in Complex with Specific Antibodies. Authors: Burger, D. / Stihle, M. / Sharma, A. / Di Lello, P. / Benz, J. / D'Arcy, B. / Debulpaep, M. / Fry, D. / Huber, W. / Kremer, T. / Laeremans, T. / Matile, H. / Ross, A. / Rufer, A.C. / Schoch, ...Authors: Burger, D. / Stihle, M. / Sharma, A. / Di Lello, P. / Benz, J. / D'Arcy, B. / Debulpaep, M. / Fry, D. / Huber, W. / Kremer, T. / Laeremans, T. / Matile, H. / Ross, A. / Rufer, A.C. / Schoch, G. / Steinmetz, M.O. / Steyaert, J. / Rudolph, M.G. / Thoma, R. / Ruf, A. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5in7.cif.gz | 49.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5in7.ent.gz | 37.9 KB | Display | PDB format |
PDBx/mmJSON format | 5in7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/in/5in7 ftp://data.pdbj.org/pub/pdb/validation_reports/in/5in7 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 12125.414 Da / Num. of mol.: 2 / Fragment: N-TERMINAL DOMAIN, UNP RESIDUES 127-231 / Mutation: K215D, K216D Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: DCX, DBCN, LISX / Production host: Escherichia coli (E. coli) / References: UniProt: O43602 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.61 Å3/Da / Density % sol: 52.88 % |
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Crystal grow | Temperature: 293 K / Method: evaporation Details: crystals were either obtained out of 20mM CAPS pH 10.5, 100 mM NaCl, 5 mM TCEP or 20 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT PH range: 7.5 - 10.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X10SA / Wavelength: 1 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: May 13, 2010 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.48→45 Å / Num. obs: 8852 / % possible obs: 99.9 % / Redundancy: 12.9 % / Biso Wilson estimate: 62.99 Å2 / CC1/2: 1 / Rsym value: 0.16 / Net I/σ(I): 9.6 |
Reflection shell | Resolution: 2.48→2.54 Å / Redundancy: 13.1 % / Mean I/σ(I) obs: 2.7 / Rsym value: 0.692 / % possible all: 99.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.48→44.69 Å / Cor.coef. Fo:Fc: 0.9387 / Cor.coef. Fo:Fc free: 0.9123 / SU R Cruickshank DPI: 0.413 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.458 / SU Rfree Blow DPI: 0.255 / SU Rfree Cruickshank DPI: 0.253
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Displacement parameters | Biso mean: 52.44 Å2
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Refine analyze | Luzzati coordinate error obs: 0.337 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.48→44.69 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.48→2.77 Å / Total num. of bins used: 5
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