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- PDB-6m64: Crystal structure of SMAD2 in complex with CBP -

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Basic information

Entry
Database: PDB / ID: 6m64
TitleCrystal structure of SMAD2 in complex with CBP
Components
  • CBP
  • Mothers against decapentaplegic homolog 2
KeywordsSIGNALING PROTEIN / TGF-beta / Complex / Transcription factor
Function / homology
Function and homology information


regulation of binding / zygotic specification of dorsal/ventral axis / paraxial mesoderm morphogenesis / homomeric SMAD protein complex / activin responsive factor complex / SMAD4 MH2 Domain Mutants in Cancer / SMAD2/3 MH2 Domain Mutants in Cancer / nodal signaling pathway / SMAD protein complex / endoderm formation ...regulation of binding / zygotic specification of dorsal/ventral axis / paraxial mesoderm morphogenesis / homomeric SMAD protein complex / activin responsive factor complex / SMAD4 MH2 Domain Mutants in Cancer / SMAD2/3 MH2 Domain Mutants in Cancer / nodal signaling pathway / SMAD protein complex / endoderm formation / heteromeric SMAD protein complex / co-SMAD binding / odontoblast differentiation / determination of left/right asymmetry in lateral mesoderm / FOXO-mediated transcription of cell cycle genes / regulation of transforming growth factor beta receptor signaling pathway / pericardium development / secondary palate development / trophoblast cell migration / SMAD2/3 Phosphorylation Motif Mutants in Cancer / TGFBR1 KD Mutants in Cancer / Transcriptional regulation of pluripotent stem cells / embryonic foregut morphogenesis / transforming growth factor beta receptor binding / Germ layer formation at gastrulation / primary miRNA processing / peptide lactyltransferase (CoA-dependent) activity / pulmonary valve morphogenesis / type I transforming growth factor beta receptor binding / Formation of definitive endoderm / SMAD protein signal transduction / NFE2L2 regulating ER-stress associated genes / protein N-acetyltransferase activity / Signaling by Activin / Activation of the TFAP2 (AP-2) family of transcription factors / NFE2L2 regulating inflammation associated genes / activin receptor signaling pathway / Formation of axial mesoderm / positive regulation of BMP signaling pathway / Signaling by NODAL / N-terminal peptidyl-lysine acetylation / regulation of smoothened signaling pathway / response to cholesterol / LRR FLII-interacting protein 1 (LRRFIP1) activates type I IFN production / NFE2L2 regulates pentose phosphate pathway genes / embryonic cranial skeleton morphogenesis / I-SMAD binding / NFE2L2 regulating MDR associated enzymes / pancreas development / MRF binding / RUNX1 regulates transcription of genes involved in differentiation of myeloid cells / aortic valve morphogenesis / signal transduction involved in regulation of gene expression / Regulation of FOXO transcriptional activity by acetylation / Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells / RUNX3 regulates NOTCH signaling / Nuclear events mediated by NFE2L2 / NOTCH4 Intracellular Domain Regulates Transcription / negative regulation of ossification / Regulation of NFE2L2 gene expression / insulin secretion / Regulation of gene expression by Hypoxia-inducible Factor / anterior/posterior pattern specification / NOTCH3 Intracellular Domain Regulates Transcription / ureteric bud development / negative regulation of transcription by RNA polymerase I / TRAF6 mediated IRF7 activation / endocardial cushion morphogenesis / NFE2L2 regulating tumorigenic genes / NFE2L2 regulating anti-oxidant/detoxification enzymes / organ growth / protein-lysine-acetyltransferase activity / adrenal gland development / embryonic digit morphogenesis / protein acetylation / homeostatic process / Notch-HLH transcription pathway / Formation of paraxial mesoderm / positive regulation of transforming growth factor beta receptor signaling pathway / SMAD binding / histone acetyltransferase activity / acetyltransferase activity / FOXO-mediated transcription of cell death genes / stimulatory C-type lectin receptor signaling pathway / Zygotic genome activation (ZGA) / TP53 Regulates Transcription of Genes Involved in Cytochrome C Release / R-SMAD binding / TGF-beta receptor signaling activates SMADs / mesoderm formation / histone acetyltransferase complex / negative regulation of cell differentiation / canonical NF-kappaB signal transduction / anatomical structure morphogenesis / cell fate commitment / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / cellular response to nutrient levels / Attenuation phase / positive regulation of double-strand break repair via homologous recombination / phosphatase binding / positive regulation of epithelial to mesenchymal transition
Similarity search - Function
MAD homology, MH1 / Dwarfin / SMAD MH1 domain superfamily / MAD homology domain 1 (MH1) profile. / SMAD domain, Dwarfin-type / MH2 domain / MAD homology domain 2 (MH2) profile. / Domain B in dwarfin family proteins / MAD homology 1, Dwarfin-type / MH1 domain ...MAD homology, MH1 / Dwarfin / SMAD MH1 domain superfamily / MAD homology domain 1 (MH1) profile. / SMAD domain, Dwarfin-type / MH2 domain / MAD homology domain 2 (MH2) profile. / Domain B in dwarfin family proteins / MAD homology 1, Dwarfin-type / MH1 domain / Domain A in dwarfin family proteins / SMAD-like domain superfamily / Nuclear receptor coactivator, CREB-bp-like, interlocking / Nuclear receptor coactivator, CREB-bp-like, interlocking domain superfamily / Creb binding / Zinc finger, TAZ-type / TAZ domain superfamily / TAZ zinc finger / Zinc finger TAZ-type profile. / TAZ zinc finger, present in p300 and CBP / : / Histone acetyltransferase p300-like, PHD domain / Coactivator CBP, KIX domain / CREB-binding protein/p300, atypical RING domain / CBP/p300-type histone acetyltransferase domain / CBP/p300, atypical RING domain superfamily / KIX domain / CREB-binding protein/p300, atypical RING domain / KIX domain profile. / CBP/p300-type histone acetyltransferase (HAT) domain profile. / Histone acetyltransferase Rtt109/CBP / Histone acetylation protein / Histone acetylation protein / Coactivator CBP, KIX domain superfamily / Zinc finger ZZ-type signature. / Zinc-binding domain, present in Dystrophin, CREB-binding protein. / Zinc finger, ZZ type / Zinc finger, ZZ-type / Zinc finger, ZZ-type superfamily / Zinc finger ZZ-type profile. / SMAD/FHA domain superfamily / Nuclear receptor coactivator, interlocking / Bromodomain, conserved site / Bromodomain signature. / Bromodomain / Bromodomain profile. / bromo domain / Bromodomain / Bromodomain-like superfamily / Zinc finger, RING/FYVE/PHD-type
Similarity search - Domain/homology
Mothers against decapentaplegic homolog 2 / CREB-binding protein
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.45 Å
AuthorsMiyazono, K. / Ito, T. / Wada, H. / Tanokura, M.
Funding support Japan, 3items
OrganizationGrant numberCountry
Japan Society for the Promotion of Science (JSPS)15K14708 Japan
Japan Society for the Promotion of Science (JSPS)17K19581 Japan
Japan Society for the Promotion of Science (JSPS)23228003 Japan
CitationJournal: Sci.Signal. / Year: 2020
Title: Structural basis for transcriptional coactivator recognition by SMAD2 in TGF-beta signaling.
Authors: Miyazono, K.I. / Ito, T. / Fukatsu, Y. / Wada, H. / Kurisaki, A. / Tanokura, M.
History
DepositionMar 13, 2020Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Nov 25, 2020Provider: repository / Type: Initial release
Revision 1.1Dec 30, 2020Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.identifier_ORCID / _citation_author.name
Revision 1.2Nov 29, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Mothers against decapentaplegic homolog 2
B: CBP
C: Mothers against decapentaplegic homolog 2
D: CBP
E: Mothers against decapentaplegic homolog 2
F: CBP


Theoretical massNumber of molelcules
Total (without water)79,8096
Polymers79,8096
Non-polymers00
Water11,782654
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area10130 Å2
ΔGint-47 kcal/mol
Surface area27770 Å2
MethodPISA
Unit cell
Length a, b, c (Å)68.371, 70.803, 165.106
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

#1: Protein Mothers against decapentaplegic homolog 2 / Mothers against DPP homolog 2 / JV18-1 / Mad-related protein 2 / hMAD-2 / SMAD family member 2 / hSMAD2


Mass: 23481.596 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: SMAD2, MADH2, MADR2 / Plasmid: pET48 / Production host: Escherichia coli (E. coli) / References: UniProt: Q15796
#2: Protein/peptide CBP


Mass: 3121.352 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Plasmid: pET48 / Production host: Escherichia coli (E. coli) / References: UniProt: Q92793*PLUS
#3: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 654 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.5 Å3/Da / Density % sol: 50.87 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.2 / Details: 0.1 M HEPES pH 7.2, 0.2 M NaCl and 31% PEG3350

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Data collection

DiffractionMean temperature: 95 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Photon Factory / Beamline: AR-NE3A / Wavelength: 1 Å
DetectorType: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Dec 1, 2018
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 1.45→47.14 Å / Num. obs: 142487 / % possible obs: 100 % / Redundancy: 13 % / CC1/2: 1 / Rpim(I) all: 0.019 / Rsym value: 0.046 / Net I/σ(I): 24.7
Reflection shellResolution: 1.45→1.47 Å / Mean I/σ(I) obs: 2.6 / Num. unique obs: 6970 / CC1/2: 0.807 / Rpim(I) all: 0.412 / Rsym value: 0.988

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Processing

Software
NameVersionClassification
PHENIX(1.12_2829: ???)refinement
XDSdata reduction
XDSdata scaling
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 5ZOJ

5zoj


Resolution: 1.45→36.673 Å / SU ML: 0.14 / Cross valid method: FREE R-VALUE / σ(F): 0.45 / Phase error: 19.51
RfactorNum. reflection% reflection
Rfree0.1893 13995 5.1 %
Rwork0.1693 --
obs0.1703 142379 99.99 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Refinement stepCycle: LAST / Resolution: 1.45→36.673 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms5190 0 0 654 5844
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0135343
X-RAY DIFFRACTIONf_angle_d1.2867270
X-RAY DIFFRACTIONf_dihedral_angle_d14.6091946
X-RAY DIFFRACTIONf_chiral_restr0.106781
X-RAY DIFFRACTIONf_plane_restr0.01957
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.45-1.46650.28434540.26848710X-RAY DIFFRACTION100
1.4665-1.48380.32274650.26088628X-RAY DIFFRACTION100
1.4838-1.50180.27784440.25368729X-RAY DIFFRACTION100
1.5018-1.52090.28294300.24298698X-RAY DIFFRACTION100
1.5209-1.54090.25025550.22878670X-RAY DIFFRACTION100
1.5409-1.5620.24654950.22018608X-RAY DIFFRACTION100
1.562-1.58430.21754690.20788663X-RAY DIFFRACTION100
1.5843-1.60790.22545370.20048633X-RAY DIFFRACTION100
1.6079-1.63310.22044630.19618684X-RAY DIFFRACTION100
1.6331-1.65980.21494830.19358666X-RAY DIFFRACTION100
1.6598-1.68850.23174780.1928705X-RAY DIFFRACTION100
1.6885-1.71920.23045330.18198529X-RAY DIFFRACTION100
1.7192-1.75220.23114770.18288741X-RAY DIFFRACTION100
1.7522-1.7880.17964310.17398712X-RAY DIFFRACTION100
1.788-1.82690.21013910.17748754X-RAY DIFFRACTION100
1.8269-1.86940.20394560.17678724X-RAY DIFFRACTION100
1.8694-1.91610.20575010.17688632X-RAY DIFFRACTION100
1.9161-1.96790.19974900.17378683X-RAY DIFFRACTION100
1.9679-2.02580.19274990.16928621X-RAY DIFFRACTION100
2.0258-2.09120.20344860.17198691X-RAY DIFFRACTION100
2.0912-2.16590.19454000.16688700X-RAY DIFFRACTION100
2.1659-2.25260.16954510.16088693X-RAY DIFFRACTION100
2.2526-2.35520.19064170.16588790X-RAY DIFFRACTION100
2.3552-2.47930.20794110.16738694X-RAY DIFFRACTION100
2.4793-2.63460.20854490.1768726X-RAY DIFFRACTION100
2.6346-2.83790.20484080.17118747X-RAY DIFFRACTION100
2.8379-3.12340.19164370.17568696X-RAY DIFFRACTION100
3.1234-3.5750.17494700.15768683X-RAY DIFFRACTION100
3.575-4.50290.15114980.14618645X-RAY DIFFRACTION100
4.5029-36.6730.16395170.15298642X-RAY DIFFRACTION100
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
16.25556.69855.50377.81746.04154.8481-0.16770.30810.1826-0.05160.4308-0.2039-0.33930.6056-0.1210.2132-0.04880.03480.3061-0.00520.2416-10.423712.7364-12.8108
22.48150.2196-0.26683.0591-0.41181.8679-0.06610.0476-0.0549-0.08360.0014-0.08090.09190.08050.07220.15210.0111-0.01370.1498-0.02380.1425-27.5886-0.9864-20.9842
36.56123.90330.91578.8169-0.27572.88580.01470.07550.1046-0.3045-0.08960.68150.0038-0.10010.06440.13980.0476-0.0080.1904-0.06030.1267-36.22122.3146-20.0761
41.8797-0.05940.29420.62090.28781.9337-0.0186-0.14780.2520.0604-0.06860.1456-0.0068-0.10640.10490.17310.0152-0.00890.1649-0.05770.2613-36.253411.3823-8.7577
54.52760.04796.35240.9977-0.37838.8882-0.2101-0.22690.5020.0578-0.14570.0614-0.2148-0.13340.4010.16310.02940.00410.1848-0.07670.2658-24.417317.7101-1.5697
66.1094.02465.50892.58283.63374.72630.183-0.0907-0.04510.0714-0.0621-0.1190.136-0.1996-0.19450.1772-0.00590.00360.2133-0.05660.2226-23.79716.3568-1.4115
72.4896-1.42810.73117.0946-3.42012.1006-0.1405-0.25050.00520.47180.07020.55710.0758-0.30310.02890.2165-0.04690.04080.2121-0.05930.2251-40.2036-2.5175-6.3359
83.84692.97612.48592.65331.90831.6593-0.07-0.08290.1058-0.0324-0.0196-0.0354-0.0326-0.05890.09830.16550.0088-0.00510.1706-0.04240.2052-17.7769.3892-6.169
91.9054-3.1071-0.79196.79660.7830.9278-0.5645-0.335-0.40122.45880.357-0.28080.84870.25210.20870.94080.0811-0.02120.3865-0.0160.3784-9.8076-2.679412.0806
107.0727-0.46365.08983.0777-0.32983.6614-0.11890.08590.32720.0741-0.02390.1576-0.25570.15130.21780.1954-0.0079-0.01870.1914-0.06830.2744-12.691922.98380.6902
113.1687-2.42510.86076.9605-1.19282.19220.1220.28770.328-0.4949-0.1155-0.0946-0.11690.1247-0.01990.2372-0.0017-0.02490.1848-0.02020.191-20.5868-6.2855-31.3191
122.62610.8802-0.33276.9435-2.04184.08880.0561-0.00520.16170.27630.078-0.1787-0.12480.0066-0.13320.12610.0014-0.04010.1521-0.03550.1373-15.3564-14.0401-25.5689
137.02780.88630.05063.3638-5.38899.4452-0.15830.30790.2884-0.10530.2447-0.4307-0.0810.2212-0.0760.18450.0222-0.01870.2686-0.06460.2003-4.783-14.4843-27.5938
144.362.7819-2.644.7469-2.7456.50230.3317-0.218-0.32540.4077-0.0628-0.03020.36950.3223-0.25750.25950.0383-0.10640.1895-0.03710.1957-11.4721-23.5405-18.3654
156.5192-6.73855.35589.0698-6.38016.2420.24930.6730.2165-0.373-0.4479-0.48160.16010.58370.16180.25990.0345-0.01370.2955-0.05320.1687-15.3378-21.1953-37.4776
167.8773-0.8130.644.40050.98281.63520.04640.0848-0.0076-0.227-0.01090.17160.045-0.0678-0.03690.2245-0.0102-0.04910.1709-0.02420.1443-27.9468-25.6785-35.6153
174.6358-5.8424-1.44228.45350.51372.40640.34430.2206-0.2369-0.704-0.15970.38780.059-0.0558-0.16610.2969-0.0063-0.09240.2063-0.02670.1598-33.2406-13.1433-41.6698
184.3507-2.28364.57883.2242-3.69435.47530.03490.0931-0.41110.06120.14250.4895-0.2547-0.1942-0.21420.2617-0.0193-0.03420.18010.00910.229-33.017-13.5768-30.4723
192.12970.303-2.44336.28261.99127.87930.15140.0297-0.46950.0488-0.19140.44660.9413-0.58130.04010.381-0.0409-0.0830.22250.0070.239-25.911-29.7971-22.4624
202.7876-3.44962.11635.6262-2.85142.1685-0.0237-0.0837-0.0909-0.00140.13080.2243-0.0385-0.1898-0.0590.22490.0069-0.03950.2128-0.01370.1433-31.7971-5.1842-32.4094
217.9475-4.3113-1.44996.7057-0.77188.21270.13340.5108-0.1183-0.5001-0.2291-0.08090.37670.02860.06510.2890.0009-0.0670.1880.01790.1563-35.7213-1.6825-45.9351
222.8231-0.3834-2.2831.16591.77057.81860.01190.23620.0652-0.09830.1703-0.15180.06040.1157-0.1750.15690.0224-0.04030.1329-0.02370.1699-6.3929-6.0036-14.0757
232.15691.4258-1.61885.4905-3.00266.1387-0.0185-0.00830.1350.03710.15630.17250.1606-0.2408-0.110.13120.0033-0.030.1283-0.0490.1768-14.27950.0822-1.9829
248.9744-2.3264-6.94992.73093.4967.13350.1965-0.24120.42240.12140.4015-0.58480.01970.6172-0.64970.19390.0436-0.0820.2583-0.12130.31013.136-2.9335-3.3631
251.4066-0.8793-1.01610.85470.96462.7035-0.1207-0.2094-0.08930.53220.4585-0.360.70480.7104-0.2970.37520.1611-0.15960.3757-0.13980.36986.0892-17.3697-5.8336
264.0532-5.0647-4.20678.06857.9268.4459-0.14110.2812-0.36260.7010.0520.20471.037-0.08770.15760.37480.0164-0.06710.2377-0.07150.2816-1.7935-20.7842-12.3131
275.4511-4.00312.68383.5316-3.70267.3562-0.022-0.4969-0.55620.66250.0826-0.16640.79070.2941-0.04720.43570.0624-0.04040.24950.0010.3157-9.783-16.24854.9082
281.398-0.5989-0.72660.92440.95313.82530.04950.415-0.31320.11270.049-0.07660.52130.0796-0.04440.22930.0305-0.06950.2354-0.11470.2608-0.2347-17.3469-19.6215
290.37061.54540.99987.17123.69463.21640.19291.0675-0.5171-0.41170.2724-0.75380.04770.8313-0.60890.2730.1076-0.05030.6061-0.32220.596713.9869-17.7782-24.1969
308.56263.44596.91975.09123.42929.4406-0.1966-0.27340.62140.123-0.4690.7738-0.269-0.83810.56550.1901-0.0167-0.01620.3314-0.11940.503319.8797-0.7088-14.711
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 265 through 274 )
2X-RAY DIFFRACTION2chain 'A' and (resid 275 through 322 )
3X-RAY DIFFRACTION3chain 'A' and (resid 323 through 341 )
4X-RAY DIFFRACTION4chain 'A' and (resid 342 through 380 )
5X-RAY DIFFRACTION5chain 'A' and (resid 381 through 400 )
6X-RAY DIFFRACTION6chain 'A' and (resid 401 through 418 )
7X-RAY DIFFRACTION7chain 'A' and (resid 419 through 436 )
8X-RAY DIFFRACTION8chain 'A' and (resid 437 through 455 )
9X-RAY DIFFRACTION9chain 'A' and (resid 456 through 467 )
10X-RAY DIFFRACTION10chain 'B' and (resid 1950 through 1968 )
11X-RAY DIFFRACTION11chain 'C' and (resid 267 through 280 )
12X-RAY DIFFRACTION12chain 'C' and (resid 281 through 300 )
13X-RAY DIFFRACTION13chain 'C' and (resid 301 through 309 )
14X-RAY DIFFRACTION14chain 'C' and (resid 310 through 332 )
15X-RAY DIFFRACTION15chain 'C' and (resid 333 through 354 )
16X-RAY DIFFRACTION16chain 'C' and (resid 355 through 380 )
17X-RAY DIFFRACTION17chain 'C' and (resid 381 through 400 )
18X-RAY DIFFRACTION18chain 'C' and (resid 401 through 418 )
19X-RAY DIFFRACTION19chain 'C' and (resid 419 through 436 )
20X-RAY DIFFRACTION20chain 'C' and (resid 437 through 460 )
21X-RAY DIFFRACTION21chain 'D' and (resid 1950 through 1969 )
22X-RAY DIFFRACTION22chain 'E' and (resid 266 through 300 )
23X-RAY DIFFRACTION23chain 'E' and (resid 301 through 332 )
24X-RAY DIFFRACTION24chain 'E' and (resid 333 through 354 )
25X-RAY DIFFRACTION25chain 'E' and (resid 355 through 400 )
26X-RAY DIFFRACTION26chain 'E' and (resid 401 through 418 )
27X-RAY DIFFRACTION27chain 'E' and (resid 419 through 436 )
28X-RAY DIFFRACTION28chain 'E' and (resid 437 through 458 )
29X-RAY DIFFRACTION29chain 'F' and (resid 1951 through 1971 )
30X-RAY DIFFRACTION30chain 'F' and (resid 1972 through 1977 )

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