+Open data
-Basic information
Entry | Database: PDB / ID: 6lz9 | |||||||||
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Title | t8E4 antibody Fab complexed with the active form of HGF | |||||||||
Components |
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Keywords | IMMUNE SYSTEM / antibody / HGF / hepatocyte growth factor / active form | |||||||||
Function / homology | Function and homology information regulation of p38MAPK cascade / regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling / Drug-mediated inhibition of MET activation / skeletal muscle cell proliferation / MET activates STAT3 / negative regulation of hydrogen peroxide-mediated programmed cell death / MET interacts with TNS proteins / MET Receptor Activation / hepatocyte growth factor receptor signaling pathway / MET receptor recycling ...regulation of p38MAPK cascade / regulation of branching involved in salivary gland morphogenesis by mesenchymal-epithelial signaling / Drug-mediated inhibition of MET activation / skeletal muscle cell proliferation / MET activates STAT3 / negative regulation of hydrogen peroxide-mediated programmed cell death / MET interacts with TNS proteins / MET Receptor Activation / hepatocyte growth factor receptor signaling pathway / MET receptor recycling / MET activates PTPN11 / MET activates RAP1 and RAC1 / myoblast proliferation / MET activates PI3K/AKT signaling / MET activates PTK2 signaling / positive regulation of DNA biosynthetic process / cellular response to hepatocyte growth factor stimulus / negative regulation of release of cytochrome c from mitochondria / chemoattractant activity / negative regulation of interleukin-6 production / positive regulation of interleukin-10 production / epithelial to mesenchymal transition / positive regulation of osteoblast differentiation / MET activates RAS signaling / negative regulation of peptidyl-serine phosphorylation / negative regulation of extrinsic apoptotic signaling pathway via death domain receptors / Interleukin-7 signaling / cell chemotaxis / negative regulation of autophagy / platelet alpha granule lumen / liver development / epithelial cell proliferation / growth factor activity / cell morphogenesis / Negative regulation of MET activity / negative regulation of inflammatory response / Constitutive Signaling by Aberrant PI3K in Cancer / positive regulation of peptidyl-tyrosine phosphorylation / Platelet degranulation / PIP3 activates AKT signaling / mitotic cell cycle / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / RAF/MAP kinase cascade / Interleukin-4 and Interleukin-13 signaling / positive regulation of MAPK cascade / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of cell migration / positive regulation of protein phosphorylation / signaling receptor binding / negative regulation of apoptotic process / positive regulation of transcription by RNA polymerase II / extracellular space / extracellular region / membrane / identical protein binding Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) Mus musculus (house mouse) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.8 Å | |||||||||
Authors | Kitago, Y. / Umitsu, M. / Takagi, J. | |||||||||
Funding support | Japan, 2items
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Citation | Journal: To Be Published Title: t8E4 antibody Fab complexed with the active form of HGF Authors: Umitsu, M. / Kitago, Y. / Takagi, J. #1: Journal: Sci Rep / Year: 2016 Title: Probing conformational and functional states of human hepatocyte growth factor by a panel of monoclonal antibodies. Authors: Umitsu, M. / Sakai, K. / Ogasawara, S. / Kaneko, M.K. / Asaki, R. / Tamura-Kawakami, K. / Kato, Y. / Matsumoto, K. / Takagi, J. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6lz9.cif.gz | 315.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6lz9.ent.gz | 214.9 KB | Display | PDB format |
PDBx/mmJSON format | 6lz9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lz/6lz9 ftp://data.pdbj.org/pub/pdb/validation_reports/lz/6lz9 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 12280.603 Da / Num. of mol.: 1 / Fragment: K4 domain / Mutation: N402Q, T476G, K491I, Q492E, L493G Source method: isolated from a genetically manipulated source Details: This molecule was produced as one polypeptide with chain B, and this chain if N-terminal portion. After the artificial enzymatic cleavage with Factor Xa, they are connected with each other by a disulfide bond. Source: (gene. exp.) Homo sapiens (human) / Gene: HGF, HPTA / Cell line (production host): Expi293F / Production host: Homo sapiens (human) / References: UniProt: P14210 |
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#2: Protein | Mass: 27107.373 Da / Num. of mol.: 1 / Fragment: SP domain / Mutation: C561S, N566Q, N653Q Source method: isolated from a genetically manipulated source Details: This molecule was produced as one polypeptide with chain A, and this chain if C-terminal portion. After the artificial enzymatic cleavage with Factor Xa, they are connected with each other by a disulfide bond. Source: (gene. exp.) Homo sapiens (human) / Gene: HGF, HPTA / Cell line (production host): Expi293F / Production host: Homo sapiens (human) / References: UniProt: P14210 |
#3: Antibody | Mass: 24075.049 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: PCA residue at the N-terminus is the self-cyclized Gln residue. Source: (gene. exp.) Mus musculus (house mouse) / Description: B cells fused with myeloma cells / Cell (production host): hybridoma / Production host: hybrid (others) |
#4: Antibody | Mass: 23847.162 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Description: B cells fused with myeloma cells / Cell (production host): hybridoma / Production host: hybrid (others) |
#5: Water | ChemComp-HOH / |
Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.15 Å3/Da / Density % sol: 61.01 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: 10 % (w/v) PEG 10000, 0.1M Magnesium acetate, 0.1M MES pH 6.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Ambient temp details: 100 / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL41XU / Wavelength: 1 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Oct 27, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→50 Å / Num. obs: 28218 / % possible obs: 99.7 % / Redundancy: 9.9 % / Biso Wilson estimate: 72.92 Å2 / CC1/2: 0.998 / Net I/σ(I): 13.8 |
Reflection shell | Resolution: 2.8→2.97 Å / Num. unique obs: 4412 / CC1/2: 0.673 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1SHY, 1OPG Resolution: 2.8→47.87 Å / SU ML: 0.5316 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 35.986 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 78.66 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.8→47.87 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 29.1260065328 Å / Origin y: 63.1646002203 Å / Origin z: 32.4677032777 Å
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Refinement TLS group | Selection details: all |