ジャーナル: Structure / 年: 2019 タイトル: Capsid Structure of a Freshwater Cyanophage Siphoviridae Mic1. 著者: Hua Jin / Yong-Liang Jiang / Feng Yang / Jun-Tao Zhang / Wei-Fang Li / Ke Zhou / Jue Ju / Yuxing Chen / Cong-Zhao Zhou / 要旨: Cyanobacteria are the most abundant photosynthetic microorganisms, the global distribution of which is mainly regulated by the corresponding cyanophages. A systematic screening of water samples in ...Cyanobacteria are the most abundant photosynthetic microorganisms, the global distribution of which is mainly regulated by the corresponding cyanophages. A systematic screening of water samples in the Lake Chaohu enabled us to isolate a freshwater siphocyanophage that infects Microcystis wesenbergii, thus termed Mic1. Using cryoelectron microscopy, we solved the 3.5-Å structure of Mic1 capsid. The major capsid protein gp40 of an HK97-like fold forms two types of capsomers, hexons and pentons. The capsomers interact with each other via the interweaved N-terminal arms of gp40 in addition to a tail-in-mouth joint along the three-fold symmetric axis, resulting in the assembly of capsid in a mortise-and-tenon pattern. The novel-fold cement protein gp47 sticks at the two-fold symmetric axis and further fixes the capsid. These findings provide structural insights into the assembly of cyanophages, and set up a platform to explore the mechanism of specific interactions and co-evolution with cyanobacteria.
A: major capsid protein B: major capsid protein C: major capsid protein D: major capsid protein E: major capsid protein F: major capsid protein U: major capsid protein V: major capsid protein W: major capsid protein X: major capsid protein Y: major capsid protein Z: major capsid protein a: major capsid protein 0: cement protein 3: cement protein 1: cement protein 4: cement protein 2: cement protein 6: cement protein 5: cement protein 7: cement protein b: cement protein 8: cement protein c: cement protein 9: cement protein d: cement protein
A: major capsid protein B: major capsid protein C: major capsid protein D: major capsid protein E: major capsid protein F: major capsid protein U: major capsid protein V: major capsid protein W: major capsid protein X: major capsid protein Y: major capsid protein Z: major capsid protein a: major capsid protein 0: cement protein 3: cement protein 1: cement protein 4: cement protein 2: cement protein 6: cement protein 5: cement protein 7: cement protein b: cement protein 8: cement protein c: cement protein 9: cement protein d: cement protein
A: major capsid protein B: major capsid protein C: major capsid protein D: major capsid protein E: major capsid protein F: major capsid protein U: major capsid protein V: major capsid protein W: major capsid protein X: major capsid protein Y: major capsid protein Z: major capsid protein a: major capsid protein 0: cement protein 3: cement protein 1: cement protein 4: cement protein 2: cement protein 6: cement protein 5: cement protein 7: cement protein b: cement protein 8: cement protein c: cement protein 9: cement protein d: cement protein
x 5
icosahedral pentamer
3.13 MDa, 130 ポリマー
分子量 (理論値)
分子数
合計 (水以外)
3,125,102
130
ポリマ-
3,125,102
130
非ポリマー
0
0
水
0
タイプ
名称
対称操作
数
identity operation
1_555
x,y,z
1
point symmetry operation
4
4
A: major capsid protein B: major capsid protein C: major capsid protein D: major capsid protein E: major capsid protein F: major capsid protein U: major capsid protein V: major capsid protein W: major capsid protein X: major capsid protein Y: major capsid protein Z: major capsid protein a: major capsid protein 0: cement protein 3: cement protein 1: cement protein 4: cement protein 2: cement protein 6: cement protein 5: cement protein 7: cement protein b: cement protein 8: cement protein c: cement protein 9: cement protein d: cement protein