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Open data
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Basic information
Entry | Database: PDB / ID: 6ini | |||||||||
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Title | a glycosyltransferase complex with UDP and the product | |||||||||
![]() | UDP-glycosyltransferase 76G1 | |||||||||
![]() | TRANSFERASE / complex | |||||||||
Function / homology | ![]() UDP-glucosyltransferase activity / Transferases; Glycosyltransferases; Hexosyltransferases Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Zhu, X. / Yang, T. / Naismith, J.H. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Hydrophobic recognition allows the glycosyltransferase UGT76G1 to catalyze its substrate in two orientations. Authors: Yang, T. / Zhang, J. / Ke, D. / Yang, W. / Tang, M. / Jiang, J. / Cheng, G. / Li, J. / Cheng, W. / Wei, Y. / Li, Q. / Naismith, J.H. / Zhu, X. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 213.1 KB | Display | ![]() |
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PDB format | ![]() | 166.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.5 MB | Display | ![]() |
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Full document | ![]() | 1.5 MB | Display | |
Data in XML | ![]() | 21.6 KB | Display | |
Data in CIF | ![]() | 31.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6infC ![]() 6ingC ![]() 6inhC ![]() 2pq6S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 53158.227 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: Q6VAB4, Transferases; Glycosyltransferases; Hexosyltransferases |
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-Non-polymers , 5 types, 277 molecules ![](data/chem/img/UDP.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/AUO.gif)
![](data/chem/img/AQ9.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/AUO.gif)
![](data/chem/img/AQ9.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-UDP / |
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#3: Chemical | ChemComp-GOL / |
#4: Chemical | ChemComp-AUO / ( |
#5: Chemical | ChemComp-AQ9 / |
#6: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 47.01 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 0.1M sodium citrate buffer at pH 5.4 and 20% PEG 4000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jan 11, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.978 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→42.88 Å / Num. obs: 54657 / % possible obs: 100 % / Redundancy: 19.1 % / Rmerge(I) obs: 0.084 / Rpim(I) all: 0.027 / Rrim(I) all: 0.088 / Net I/σ(I): 18.9 |
Reflection shell | Resolution: 1.7→1.74 Å / Rmerge(I) obs: 1.139 / Num. unique obs: 4025 / Rpim(I) all: 0.38 / Rrim(I) all: 1.201 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2PQ6 Resolution: 1.7→42.88 Å / Cor.coef. Fo:Fc: 0.974 / Cor.coef. Fo:Fc free: 0.965 / SU B: 4.25 / SU ML: 0.067 / Cross valid method: THROUGHOUT / ESU R: 0.095 / ESU R Free: 0.093 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.9 Å / Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 31.239 Å2
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Refinement step | Cycle: 1 / Resolution: 1.7→42.88 Å
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Refine LS restraints |
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