+Open data
-Basic information
Entry | Database: PDB / ID: 6inf | ||||||
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Title | a glycosyltransferase complex with UDP | ||||||
Components | UDP-glycosyltransferase 76G1 | ||||||
Keywords | TRANSFERASE / complex | ||||||
Function / homology | Function and homology information UDP-glucosyltransferase activity / Transferases; Glycosyltransferases; Hexosyltransferases Similarity search - Function | ||||||
Biological species | Stevia rebaudiana (plant) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.69 Å | ||||||
Authors | Zhu, X. / Yang, T. / Naismith, J.H. | ||||||
Funding support | China, 1items
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Citation | Journal: Nat Commun / Year: 2019 Title: Hydrophobic recognition allows the glycosyltransferase UGT76G1 to catalyze its substrate in two orientations. Authors: Yang, T. / Zhang, J. / Ke, D. / Yang, W. / Tang, M. / Jiang, J. / Cheng, G. / Li, J. / Cheng, W. / Wei, Y. / Li, Q. / Naismith, J.H. / Zhu, X. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6inf.cif.gz | 202.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6inf.ent.gz | 160.6 KB | Display | PDB format |
PDBx/mmJSON format | 6inf.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/in/6inf ftp://data.pdbj.org/pub/pdb/validation_reports/in/6inf | HTTPS FTP |
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-Related structure data
Related structure data | 6ingC 6inhC 6iniC 2pq6S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 53158.227 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Stevia rebaudiana (plant) / Gene: UGT76G1 / Production host: Escherichia coli (E. coli) References: UniProt: Q6VAB4, Transferases; Glycosyltransferases; Hexosyltransferases |
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#2: Chemical | ChemComp-UDP / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 47.01 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 0.1M sodium citrate buffer at pH 5.4 and 20% PEG 4000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL19U1 / Wavelength: 0.978 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Dec 14, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.978 Å / Relative weight: 1 |
Reflection | Resolution: 1.69→49.58 Å / Num. obs: 56938 / % possible obs: 100 % / Redundancy: 19.2 % / CC1/2: 0.999 / Rmerge(I) obs: 0.069 / Rpim(I) all: 0.022 / Rrim(I) all: 0.072 / Net I/σ(I): 20.2 |
Reflection shell | Resolution: 1.69→1.73 Å / Rmerge(I) obs: 1.007 / Mean I/σ(I) obs: 4 / Num. measured obs: 81062 / Num. unique obs: 4173 / CC1/2: 0.948 / Rpim(I) all: 0.333 / Rrim(I) all: 1.061 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2PQ6 Resolution: 1.69→48.83 Å / Cor.coef. Fo:Fc: 0.968 / Cor.coef. Fo:Fc free: 0.954 / SU B: 4.158 / SU ML: 0.07 / Cross valid method: THROUGHOUT / ESU R: 0.097 / ESU R Free: 0.095 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 38.083 Å2
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Refinement step | Cycle: 1 / Resolution: 1.69→48.83 Å
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Refine LS restraints |
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