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- PDB-6ias: structure of human NKp46 in complex with antibody NKp46-1 and NKp46-4 -
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Open data
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Basic information
Entry | Database: PDB / ID: 6ias | ||||||
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Title | structure of human NKp46 in complex with antibody NKp46-1 and NKp46-4 | ||||||
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![]() | IMMUNE SYSTEM / THERAPEUTIC ANTIBODY | ||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta![]() | ||||||
Biological species | synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
![]() | Roussel, A. / Amigues, B. | ||||||
![]() | ![]() Title: Multifunctional Natural Killer Cell Engagers Targeting NKp46 Trigger Protective Tumor Immunity. Authors: Gauthier, L. / Morel, A. / Anceriz, N. / Rossi, B. / Blanchard-Alvarez, A. / Grondin, G. / Trichard, S. / Cesari, C. / Sapet, M. / Bosco, F. / Rispaud-Blanc, H. / Guillot, F. / Cornen, S. / ...Authors: Gauthier, L. / Morel, A. / Anceriz, N. / Rossi, B. / Blanchard-Alvarez, A. / Grondin, G. / Trichard, S. / Cesari, C. / Sapet, M. / Bosco, F. / Rispaud-Blanc, H. / Guillot, F. / Cornen, S. / Roussel, A. / Amigues, B. / Habif, G. / Caraguel, F. / Arrufat, S. / Remark, R. / Romagne, F. / Morel, Y. / Narni-Mancinelli, E. / Vivier, E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 182.4 KB | Display | ![]() |
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PDB format | ![]() | 149.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 423.1 KB | Display | ![]() |
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Full document | ![]() | 427.3 KB | Display | |
Data in XML | ![]() | 21 KB | Display | |
Data in CIF | ![]() | 31.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 23233.980 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: ![]() ![]() |
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#2: Antibody | Mass: 23244.791 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: ![]() ![]() |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.6 Å3/Da / Density % sol: 52.75 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: 0.1M MMT pH8.5-9.5, 20-30%(w/v) PEG 1500 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 9M / Detector: PIXEL / Date: Mar 30, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9801 Å / Relative weight: 1 |
Reflection | Resolution: 1.725→37.25 Å / Num. obs: 50086 / % possible obs: 99.5 % / Redundancy: 6.9 % / Biso Wilson estimate: 32.64 Å2 / Net I/σ(I): 8.8 |
Reflection shell | Resolution: 1.725→1.82 Å |
-Phasing
Phasing | Method: ![]() |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso max: 134.01 Å2 / Biso mean: 41.55 Å2 / Biso min: 17.03 Å2
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Refine analyze | Luzzati coordinate error obs: 0.24 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.75→36.76 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.75→1.79 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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