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- PDB-6f5p: A mechanism for the activation of the influenza virus transcriptase -

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ID or keywords:

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Basic information

Entry
Database: PDB / ID: 6f5p
TitleA mechanism for the activation of the influenza virus transcriptase
Components
  • ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA
  • DNA-directed RNA polymerase subunitPolymerase
  • Polymerase acidic protein
  • Polymerase basic protein 2
  • RNA-directed RNA polymerase catalytic subunit
KeywordsREPLICATION / influenza virus RNA polymerase / Pol II / transcription
Function / homology
Function and homology information


microfibril binding / Abortive elongation of HIV-1 transcript in the absence of Tat / FGFR2 alternative splicing / MicroRNA (miRNA) biogenesis / Viral Messenger RNA Synthesis / Signaling by FGFR2 IIIa TM / RNA Pol II CTD phosphorylation and interaction with CE during HIV infection / RNA Pol II CTD phosphorylation and interaction with CE / symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity / Formation of the Early Elongation Complex ...microfibril binding / Abortive elongation of HIV-1 transcript in the absence of Tat / FGFR2 alternative splicing / MicroRNA (miRNA) biogenesis / Viral Messenger RNA Synthesis / Signaling by FGFR2 IIIa TM / RNA Pol II CTD phosphorylation and interaction with CE during HIV infection / RNA Pol II CTD phosphorylation and interaction with CE / symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity / Formation of the Early Elongation Complex / Formation of the HIV-1 Early Elongation Complex / mRNA Capping / cap snatching / PIWI-interacting RNA (piRNA) biogenesis / mRNA Splicing - Minor Pathway / HIV Transcription Initiation / RNA Polymerase II HIV Promoter Escape / Transcription of the HIV genome / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / 7-methylguanosine mRNA capping / viral transcription / Processing of Capped Intron-Containing Pre-mRNA / Pausing and recovery of Tat-mediated HIV elongation / Tat-mediated HIV elongation arrest and recovery / RNA polymerase II activity / RNA polymerase II transcribes snRNA genes / HIV elongation arrest and recovery / Pausing and recovery of HIV elongation / Tat-mediated elongation of the HIV-1 transcript / Formation of HIV-1 elongation complex containing HIV-1 Tat / Formation of HIV elongation complex in the absence of HIV Tat / RNA polymerase II, core complex / RNA Polymerase II Transcription Elongation / Formation of RNA Pol II elongation complex / RNA Polymerase II Pre-transcription Events / Inhibition of DNA recombination at telomere / mRNA Splicing - Major Pathway / positive regulation of RNA splicing / virion component / promoter-specific chromatin binding / DNA-templated transcription termination / TP53 Regulates Transcription of DNA Repair Genes / Transcriptional regulation by small RNAs / Transcription-Coupled Nucleotide Excision Repair (TC-NER) / Formation of TC-NER Pre-Incision Complex / kinase binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / Activation of anterior HOX genes in hindbrain development during early embryogenesis / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / chromosome / Hydrolases; Acting on ester bonds; Exoribonucleases producing 5'-phosphomonoesters / endonuclease activity / Estrogen-dependent gene expression / host cell cytoplasm / transcription by RNA polymerase II / Hydrolases; Acting on ester bonds / RNA-directed RNA polymerase / viral RNA genome replication / RNA-dependent RNA polymerase activity / nucleotide binding / DNA-templated transcription / host cell nucleus / ubiquitin protein ligase binding / regulation of DNA-templated transcription / DNA binding / RNA binding / nucleoplasm / metal ion binding / nucleus / cytoplasm
Similarity search - Function
Influenza RNA-dependent RNA polymerase subunit PB1 / Influenza RNA-dependent RNA polymerase subunit PB1 / Influenza RNA-dependent RNA polymerase subunit PB2 / : / : / : / : / : / Influenza RNA polymerase PB2 N-terminal region / Influenza RNA polymerase PB2 second domain ...Influenza RNA-dependent RNA polymerase subunit PB1 / Influenza RNA-dependent RNA polymerase subunit PB1 / Influenza RNA-dependent RNA polymerase subunit PB2 / : / : / : / : / : / Influenza RNA polymerase PB2 N-terminal region / Influenza RNA polymerase PB2 second domain / Influenza RNA polymerase PB2 middle domain / Influenza RNA polymerase PB2 6th domain / Influenza RNA polymerase PB2 C-terminal domain / : / : / Influenza RNA polymerase PB2 helical domain / Influenza RNA polymerase PB2 CAP binding domain / Polymerase acidic protein / Influenza RNA-dependent RNA polymerase subunit PA / Influenza RNA-dependent RNA polymerase subunit PA, endonuclease domain / Influenza RNA-dependent RNA polymerase subunit PA / RNA-directed RNA polymerase, negative-strand RNA virus / RdRp of negative ssRNA viruses with segmented genomes catalytic domain profile. / RNA polymerase II, heptapeptide repeat, eukaryotic / RNA polymerase Rpb1, domain 6 / RNA polymerase Rpb1, domain 6 / RNA polymerase Rpb1 C-terminal repeat / Eukaryotic RNA polymerase II heptapeptide repeat. / RNA polymerase Rpb1, domain 7 / RNA polymerase Rpb1, domain 7 superfamily / RNA polymerase Rpb1, domain 7 / RNA polymerase Rpb1, domain 3 superfamily / RNA polymerase Rpb1, clamp domain superfamily / DNA-directed RNA polymerase, subunit beta-prime / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 3 / RNA polymerase Rpb1, domain 1 / RNA polymerase Rpb1, domain 1 / RNA polymerase, alpha subunit / RNA polymerase Rpb1, domain 4 / RNA polymerase Rpb1, domain 2 / RNA polymerase Rpb1, domain 4 / RNA polymerase, N-terminal / RNA polymerase Rpb1, funnel domain superfamily / RNA polymerase I subunit A N-terminus / RNA polymerase Rpb1, domain 5 / RNA polymerase Rpb1, domain 5
Similarity search - Domain/homology
DNA-directed RNA polymerase II subunit RPB1 / Polymerase basic protein 2 / RNA-directed RNA polymerase catalytic subunit / Polymerase acidic protein
Similarity search - Component
Biological speciesInfluenza C virus
Fopius arisanus (insect)
Homo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / Resolution: 4.14 Å
AuthorsSerna Martin, I. / Grimes, J.M.
Funding support United Kingdom, 3items
OrganizationGrant numberCountry
Medical Research Council (United Kingdom)MR/K000241/1 United Kingdom
Wellcome Trust200835/Z/16/Z United Kingdom
Wellcome Trust092931/Z/10/Z United Kingdom
CitationJournal: Mol Cell / Year: 2018
Title: A Mechanism for the Activation of the Influenza Virus Transcriptase.
Authors: Itziar Serna Martin / Narin Hengrung / Max Renner / Jane Sharps / Mónica Martínez-Alonso / Simonas Masiulis / Jonathan M Grimes / Ervin Fodor /
Abstract: Influenza virus RNA polymerase (FluPol), a heterotrimer composed of PB1, PB2, and PA subunits (P3 in influenza C), performs both transcription and replication of the viral RNA genome. For ...Influenza virus RNA polymerase (FluPol), a heterotrimer composed of PB1, PB2, and PA subunits (P3 in influenza C), performs both transcription and replication of the viral RNA genome. For transcription, FluPol interacts with the C-terminal domain (CTD) of RNA polymerase II (Pol II), which enables FluPol to snatch capped RNA primers from nascent host RNAs. Here, we describe the co-crystal structure of influenza C virus polymerase (FluPol) bound to a Ser5-phosphorylated CTD (pS-CTD) peptide. The position of the CTD-binding site at the interface of PB1, P3, and the flexible PB2 C-terminal domains suggests that CTD binding stabilizes the transcription-competent conformation of FluPol. In agreement, both cap snatching and capped primer-dependent transcription initiation by FluPol are enhanced in the presence of pS-CTD. Mutations of amino acids in the CTD-binding site reduce viral mRNA synthesis. We propose a model for the activation of the influenza virus transcriptase through its association with pS-CTD of Pol II.
History
DepositionDec 2, 2017Deposition site: PDBE / Processing site: PDBE
Revision 1.0Sep 19, 2018Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Polymerase acidic protein
D: Polymerase acidic protein
B: RNA-directed RNA polymerase catalytic subunit
C: RNA-directed RNA polymerase catalytic subunit
E: Polymerase basic protein 2
F: Polymerase basic protein 2
G: DNA-directed RNA polymerase subunit
H: ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA
hetero molecules


Theoretical massNumber of molelcules
Total (without water)516,28512
Polymers516,1878
Non-polymers974
Water0
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: homology, SAXS, gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area77730 Å2
ΔGint-443 kcal/mol
Surface area182040 Å2
MethodPISA
Unit cell
Length a, b, c (Å)185.700, 185.700, 597.590
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number96
Space group name H-MP43212
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-ID
11
21
12
22
13
23

NCS domain segments:
Dom-IDComponent-IDEns-IDSelection details
111(chain A and (resseq 1:47 or (resid 48 and (name...
211(chain D and (resseq 1:47 or (resid 48 and (name...
112(chain B and (resseq 1:191 or resseq 203:230 or resseq...
212(chain C and (resseq 1:191 or resseq 203:230 or resseq...
113(chain F and (resseq 1:49 or resseq 51:249 or resseq 251:771))
213(chain E and (resseq 1:49 or resseq 51:249 or resseq 251:771))

NCS ensembles :
ID
1
2
3

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Components

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Protein , 3 types, 6 molecules ADBCEF

#1: Protein Polymerase acidic protein / RNA-directed RNA polymerase subunit P2


Mass: 82025.531 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Influenza C virus (strain C/Johannesburg/1/1966)
Strain: C/Johannesburg/1/1966 / Gene: PA, P3 / Production host: Spodoptera frugiperda (fall armyworm)
References: UniProt: Q9IMP5, Hydrolases; Acting on ester bonds
#2: Protein RNA-directed RNA polymerase catalytic subunit / Polymerase basic protein 1 / PB1 / RNA-directed RNA polymerase subunit P1


Mass: 86145.945 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Influenza C virus (strain C/Johannesburg/1/1966)
Strain: C/Johannesburg/1/1966 / Gene: PB1 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: Q9IMP4, RNA-directed RNA polymerase
#3: Protein Polymerase basic protein 2 / RNA-directed RNA polymerase subunit P3


Mass: 87949.422 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Influenza C virus (strain C/Johannesburg/1/1966)
Strain: C/Johannesburg/1/1966 / Gene: PB2 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: Q9IMP3

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Protein/peptide , 2 types, 2 molecules GH

#4: Protein/peptide DNA-directed RNA polymerase subunit / Polymerase


Mass: 3216.893 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Fopius arisanus (insect) / Gene: RpII215_0, g.45518 / Production host: synthetic construct (others)
References: UniProt: P24928*PLUS, DNA-directed RNA polymerase
#5: Protein/peptide ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA-ALA


Mass: 728.793 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human)

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Non-polymers , 1 types, 4 molecules

#6: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Mg

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 4.99 Å3/Da / Density % sol: 75.35 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop
Details: 70% (v/v) Morpheus G2 (Molecular Dimensions) and 7.5% (v/v) glycerol

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I04 / Wavelength: 0.9763 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jul 7, 2014
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9763 Å / Relative weight: 1
ReflectionResolution: 4.1→132.4 Å / Num. obs: 75524 / % possible obs: 100 % / Redundancy: 13.5 % / CC1/2: 0.998 / Rpim(I) all: 0.055 / Net I/σ(I): 7.9
Reflection shellResolution: 4.14→4.21 Å / Redundancy: 2.1 % / Mean I/σ(I) obs: 0.9 / CC1/2: 0.733 / Rpim(I) all: 0.568 / % possible all: 60.6

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Processing

Software
NameVersionClassification
PHENIX(1.10.1_2155: ???)refinement
xia2data reduction
xia2data scaling
PHENIXphasing
RefinementResolution: 4.14→131.31 Å / SU ML: 0.64 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 31.93
RfactorNum. reflection% reflection
Rfree0.2958 2816 5.06 %
Rwork0.26 --
obs0.2618 55611 68.9 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Refinement stepCycle: LAST / Resolution: 4.14→131.31 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms35282 0 0 0 35282
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00535891
X-RAY DIFFRACTIONf_angle_d0.81248264
X-RAY DIFFRACTIONf_dihedral_angle_d16.88322148
X-RAY DIFFRACTIONf_chiral_restr0.055302
X-RAY DIFFRACTIONf_plane_restr0.0056176
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDTypeRms dev position (Å)
11A5622X-RAY DIFFRACTIONPOSITIONAL
12D5622X-RAY DIFFRACTIONPOSITIONAL0.067
21B5636X-RAY DIFFRACTIONPOSITIONAL
22C5636X-RAY DIFFRACTIONPOSITIONAL0.057
31F6120X-RAY DIFFRACTIONPOSITIONAL
32E6120X-RAY DIFFRACTIONPOSITIONAL0.041
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
16.26890.46391.04094.9626-0.24362.83320.09960.3430.1464-0.0954-0.0737-0.0619-0.55251.0157-0.00981.2621-0.2696-0.19811.2916-0.26820.831226.5964-28.44487.7486
20.84690.4720.24181.64070.631.2949-0.19640.1640.1594-0.48680.1888-0.0217-0.14460.10480.12250.8606-0.1402-0.18911.13450.14040.7974-4.7957-77.2638-26.9135
34.9645-2.5896-1.50475.72072.40073.2741-0.1836-0.20890.1026-0.31730.30830.36780.6293-0.0256-0.1150.7438-0.3576-0.19650.84540.2040.703-15.0044-90.1109-20.3334
46.2489-0.58950.84852.6398-1.62333.2083-0.34660.1356-0.5493-0.23980.2736-0.71081.36130.73240.07481.73560.63550.24791.40780.42321.487114.5107-154.8666-60.1149
51.3026-0.48520.45273.5301-1.29642.86270.55290.6794-0.0638-0.7411-1.0117-1.00250.24891.57740.29870.48190.01190.28271.96080.23590.641518.9817-115.1242-95.4539
64.45872.9814-1.26726.3235-0.61417.2876-0.46220.9727-1.517-1.04560.42680.67881.1202-1.3460.05441.2354-0.40810.0861.3563-0.12851.0919-20.2236-87.92-112.9827
70.8377-0.5285-2.31743.59930.99824.11920.29450.17030.1186-0.9067-0.4566-0.1549-1.41270.13120.11121.2256-0.1119-0.03060.85110.03750.8137-0.072-92.5993-100.1314
86.77561.04024.43082.2731-0.55996.0991-0.1180.01050.4511-0.0042-0.0452-0.1901-1.37370.24340.07581.1442-0.30130.140.80160.01140.6774-4.4691-83.2624-95.1976
95.386-2.28730.25453.935-1.04583.78770.3140.41410.35530.00550.0524-0.8495-0.68360.8952-0.00371.0231-0.46060.13841.35880.23790.990922.3711-89.3646-98.604
105.54579.6858-3.95843.5081-9.4415.14210.5864-0.0069-0.68140.5525-0.4441-0.2569-1.44480.2857-0.08541.2885-0.23110.16611.1250.08641.537-14.4874-71.8848-24.4477
115.9174-0.8786-2.6192.170.58965.0133-0.1186-0.1357-0.3046-0.35570.1757-0.1595-0.060.3752-0.13360.931-0.3877-0.29280.7436-0.1040.92749.2452-49.0683-25.5375
121.4744-0.705-0.81721.7609-0.83012.7846-0.3390.13410.31660.03860.1799-0.0182-0.2199-0.59950.23440.5679-0.1794-0.18380.8106-0.04850.7128-5.8022-52.9596-22.2225
134.561-1.9181-2.59575.36114.45941.9713-0.18390.7181-0.6493-0.8394-0.27650.55260.1294-0.25250.18581.0531-0.3657-0.24040.74760.18911.035-0.5289-56.0544-43.718
147.8533-3.24951.72674.6516-0.28944.1929-0.3002-0.3642-0.59450.04610.36971.4817-0.4482-0.7209-0.00120.7520.0659-0.11540.8148-0.02140.9235-18.663-55.2669-13.7068
158.1318-1.4941-0.59729.51924.11848.57640.8197-0.4834-0.6159-0.80490.5272-1.34390.69570.3217-1.25090.83180.00690.00950.78080.05030.7216.1574-89.49191.2211
164.22453.7954-3.45139.4122-1.81144.9066-0.39050.6859-1.7438-0.576-0.3595-1.58021.12010.18610.53340.94890.12170.00731.2054-0.05861.55334.1267-68.8491-6.1967
17-1.4942-0.7722-0.61137.78137.14672.1278-0.04440.11570.212-0.9318-0.0057-0.3371-1.01881.26680.15331.377-0.3412-0.06381.33790.18031.104315.1462-98.6349-98.6611
185.67782.766-0.59626.0088-0.10293.80730.48580.51120.019-0.165-0.4749-0.0750.70540.49580.07520.90440.26250.07870.91260.2090.745713.5835-132.4994-96.9733
19-2.10251.46030.10154.72622.82953.8377-0.11980.11520.20590.2751-0.29160.1907-0.31781.5210.37980.94760.06070.09962.05450.33891.028721.9534-113.8849-87.8564
205.8238-3.16612.91612.3444-1.25056.22830.1292-0.16140.032-0.113-0.2834-0.3260.25911.02010.01780.73920.1280.0361.01750.16770.829415.7993-124.4026-108.7753
210.8485-1.295-0.23522.4925-0.56591.95320.37160.15410.9919-0.0011-0.417-0.8256-0.28371.42790.02411.1066-0.42450.11452.22840.28291.29329.5302-106.5063-86.8248
227.6314-0.10643.8694.5365-1.980711.03450.7867-0.168-0.31870.337-0.31720.97330.582-0.5924-0.39921.1946-0.5304-0.07750.81820.10220.8195-12.1885-97.2751-73.2404
230.6644-3.87790.31658.63033.45983.7142-0.16280.5089-0.1519-0.4707-0.6762.0082-0.1722-0.4390.73911.1792-0.4674-0.14131.4484-0.05341.3877-18.1301-132.1405-76.8395
241.31971.7768-0.9998.5008-2.2844-0.3696-0.58410.4465-0.1767-0.17410.6148-0.15270.6523-0.24840.16660.9914-0.04330.03622.0726-0.23451.242528.8939-71.5539-12.4663
251.3753-0.0303-0.71448.40333.61426.4882-0.14140.27060.2931-1.47270.6856-1.8564-0.57920.8732-0.35281.1666-0.13610.24311.101-0.00980.901613.5767-80.96524.6727
263.86773.56811.10689.77053.57298.0128-0.49410.2085-0.9415-0.90351.05240.0438-0.57291.4373-0.74320.7192-0.0644-0.35691.3620.26711.421823.9963-63.265111.8385
273.18854.2582.49378.97283.46513.2570.3279-1.6751.02460.0347-0.72260.8956-0.3418-0.69990.48190.94070.25070.12291.1073-0.17890.9453-4.503-70.485613.0198
287.6459-0.57282.55543.7057-2.4295.8534-0.8277-0.55430.22461.1520.66190.0209-0.70970.05240.23921.16890.10130.04070.8471-0.20720.9338-1.2109-43.855521.3914
296.8779-1.64932.16938.54741.12016.3129-0.5725-0.44560.29231.20140.15651.015-0.739-0.75540.33621.2130.24790.11111.19730.07771.0114-23.3549-46.035219.1712
302.85970.30893.12830.2301-1.79714.8655-0.0328-0.54820.98020.3061-0.3954-0.3308-0.09550.46010.55511.60360.4265-0.14621.5175-0.46691.4637.8173-44.346628.6648
31-0.7242-1.4295-1.58497.3053-4.46744.8220.45-1.0449-0.12532.46960.2335-0.76280.09492.9542-0.56331.9256-0.2719-0.50062.4257-0.21571.748735.2566-55.373939.6338
322.7873-1.84150.89779.1137-0.83162.6060.5991-0.3806-0.11540.9275-0.3366-1.63080.06351.0302-0.26691.0536-0.2761-0.42262.17450.17731.199735.6328-44.964414.023
331.8922-0.6213-0.29884.30180.61331.205-0.47160.36110.5782-0.75310.32540.66610.174-0.1290.17711.5712-0.5957-0.09631.5024-0.00761.0186-16.2666-126.9327-83.795
342.9563-0.76191.5872.2969-1.20035.99980.58720.3878-0.3174-0.4178-0.19890.93080.8269-0.0841-0.17520.9983-0.26850.01750.83720.04251.2074-11.5203-107.9535-68.5228
351.53493.1756-1.25934.9602-3.70287.3796-0.0622-0.4582-0.38040.35840.2153-0.16380.19960.8234-0.23840.93680.14220.01881.32260.03970.9882-0.4361-116.8669-59.9147
363.3957-0.1072-1.4813.02380.11995.7291-0.4531-0.7602-0.0870.1409-0.0214-0.79480.62161.78960.34880.97580.1436-0.30871.50380.25281.035228.0553-118.401-52.904
371.8006-0.01720.56580.15980.10941.59770.1929-0.5338-0.12750.4718-0.1302-0.1547-0.08071.43340.01571.45920.0524-0.08222.34160.22711.440430.8823-115.9722-47.181
383.65032.6453.17586.89774.5724.6622-0.0236-0.4705-0.03160.3558-0.3429-0.49060.4745-0.88040.44371.3088-0.22130.15031.20760.42631.2739-10.0176-137.7539-30.3537
397.3856-2.036-1.70784.5064-0.20446.72340.2607-0.2784-0.80360.35470.16140.681.02510.8966-0.41911.8293-0.25150.23761.08340.06890.8754-3.1343-148.17-55.0216
402.3222-5.12450.78749.9115-2.34210.71090.59450.14742.20441.89051.43941.4015-0.8598-0.1206-0.97691.422-0.4585-0.0031.87580.01632.8819-41.2895-60.046-15.6557
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 167 )
2X-RAY DIFFRACTION2chain 'A' and (resid 168 through 429 )
3X-RAY DIFFRACTION3chain 'A' and (resid 430 through 708 )
4X-RAY DIFFRACTION4chain 'D' and (resid 1 through 167 )
5X-RAY DIFFRACTION5chain 'D' and (resid 168 through 284 )
6X-RAY DIFFRACTION6chain 'D' and (resid 285 through 343 )
7X-RAY DIFFRACTION7chain 'D' and (resid 344 through 429 )
8X-RAY DIFFRACTION8chain 'D' and (resid 430 through 633 )
9X-RAY DIFFRACTION9chain 'D' and (resid 634 through 708 )
10X-RAY DIFFRACTION10chain 'B' and (resid 1 through 49 )
11X-RAY DIFFRACTION11chain 'B' and (resid 50 through 267 )
12X-RAY DIFFRACTION12chain 'B' and (resid 268 through 374 )
13X-RAY DIFFRACTION13chain 'B' and (resid 375 through 413 )
14X-RAY DIFFRACTION14chain 'B' and (resid 414 through 519 )
15X-RAY DIFFRACTION15chain 'B' and (resid 520 through 618 )
16X-RAY DIFFRACTION16chain 'B' and (resid 619 through 753 )
17X-RAY DIFFRACTION17chain 'C' and (resid 1 through 49 )
18X-RAY DIFFRACTION18chain 'C' and (resid 50 through 223 )
19X-RAY DIFFRACTION19chain 'C' and (resid 224 through 316 )
20X-RAY DIFFRACTION20chain 'C' and (resid 317 through 413 )
21X-RAY DIFFRACTION21chain 'C' and (resid 414 through 519 )
22X-RAY DIFFRACTION22chain 'C' and (resid 520 through 618 )
23X-RAY DIFFRACTION23chain 'C' and (resid 619 through 753 )
24X-RAY DIFFRACTION24chain 'E' and (resid 1 through 71 )
25X-RAY DIFFRACTION25chain 'E' and (resid 72 through 162 )
26X-RAY DIFFRACTION26chain 'E' and (resid 163 through 217 )
27X-RAY DIFFRACTION27chain 'E' and (resid 218 through 258 )
28X-RAY DIFFRACTION28chain 'E' and (resid 259 through 370 )
29X-RAY DIFFRACTION29chain 'E' and (resid 371 through 506 )
30X-RAY DIFFRACTION30chain 'E' and (resid 507 through 574 )
31X-RAY DIFFRACTION31chain 'E' and (resid 575 through 716 )
32X-RAY DIFFRACTION32chain 'E' and (resid 717 through 771 )
33X-RAY DIFFRACTION33chain 'F' and (resid 1 through 71 )
34X-RAY DIFFRACTION34chain 'F' and (resid 72 through 162 )
35X-RAY DIFFRACTION35chain 'F' and (resid 163 through 258 )
36X-RAY DIFFRACTION36chain 'F' and (resid 259 through 417 )
37X-RAY DIFFRACTION37chain 'F' and (resid 418 through 574 )
38X-RAY DIFFRACTION38chain 'F' and (resid 575 through 716 )
39X-RAY DIFFRACTION39chain 'F' and (resid 717 through 771 )
40X-RAY DIFFRACTION40chain 'G' and (resid 104 through 115 )

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