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- PDB-6f5o: A mechanism for the activation of the influenza virus transcriptase -

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Basic information

Entry
Database: PDB / ID: 6f5o
TitleA mechanism for the activation of the influenza virus transcriptase
Components
  • 3' promoter vRNA
  • 5' promoter vRNA
  • Polymerase acidic protein
  • Polymerase basic protein 2
  • RNA-directed RNA polymerase catalytic subunit
KeywordsVIRAL PROTEIN / influenza virus RNA dependent RNA polymerase
Function / homologyInfluenza RNA-dependent RNA polymerase subunit PA / RdRp of negative ssRNA viruses with segmented genomes catalytic domain profile. / Influenza RNA-dependent RNA polymerase subunit PB2 / Influenza RNA-dependent RNA polymerase subunit PA / Influenza RNA-dependent RNA polymerase subunit PB1 / PA/PA-X superfamily / Polymerase acidic protein / PB2, C-terminal / RNA-directed RNA polymerase, negative-strand RNA virus / Influenza RNA-dependent RNA polymerase subunit PB2 ...Influenza RNA-dependent RNA polymerase subunit PA / RdRp of negative ssRNA viruses with segmented genomes catalytic domain profile. / Influenza RNA-dependent RNA polymerase subunit PB2 / Influenza RNA-dependent RNA polymerase subunit PA / Influenza RNA-dependent RNA polymerase subunit PB1 / PA/PA-X superfamily / Polymerase acidic protein / PB2, C-terminal / RNA-directed RNA polymerase, negative-strand RNA virus / Influenza RNA-dependent RNA polymerase subunit PB2 / Influenza RNA-dependent RNA polymerase subunit PB1 / viral transcription / cap snatching / suppression by virus of host RNA polymerase II activity / 7-methylguanosine mRNA capping / virion / RNA-directed RNA polymerase / endonuclease activity / viral RNA genome replication / RNA-directed 5'-3' RNA polymerase activity / host cell cytoplasm / Hydrolases, Acting on ester bonds / transcription, DNA-templated / host cell nucleus / nucleotide binding / RNA binding / metal ion binding / Polymerase basic protein 2 / RNA-directed RNA polymerase catalytic subunit / Polymerase acidic protein
Function and homology information
Specimen sourceInfluenza B virus
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / 9.8 Å resolution
AuthorsSerna Martin, I. / Grimes, J.M.
CitationJournal: Mol. Cell / Year: 2018
Title: A Mechanism for the Activation of the Influenza Virus Transcriptase.
Authors: Itziar Serna Martin / Narin Hengrung / Max Renner / Jane Sharps / Mónica Martínez-Alonso / Simonas Masiulis / Jonathan M Grimes / Ervin Fodor
Abstract: Influenza virus RNA polymerase (FluPol), a heterotrimer composed of PB1, PB2, and PA subunits (P3 in influenza C), performs both transcription and replication of the viral RNA genome. For ...Influenza virus RNA polymerase (FluPol), a heterotrimer composed of PB1, PB2, and PA subunits (P3 in influenza C), performs both transcription and replication of the viral RNA genome. For transcription, FluPol interacts with the C-terminal domain (CTD) of RNA polymerase II (Pol II), which enables FluPol to snatch capped RNA primers from nascent host RNAs. Here, we describe the co-crystal structure of influenza C virus polymerase (FluPol) bound to a Ser5-phosphorylated CTD (pS-CTD) peptide. The position of the CTD-binding site at the interface of PB1, P3, and the flexible PB2 C-terminal domains suggests that CTD binding stabilizes the transcription-competent conformation of FluPol. In agreement, both cap snatching and capped primer-dependent transcription initiation by FluPol are enhanced in the presence of pS-CTD. Mutations of amino acids in the CTD-binding site reduce viral mRNA synthesis. We propose a model for the activation of the influenza virus transcriptase through its association with pS-CTD of Pol II.
Validation Report
SummaryFull reportAbout validation report
DateDeposition: Dec 2, 2017 / Release: Jun 27, 2018
RevisionDateData content typeGroupCategoryItemProviderType
1.0Jun 27, 2018Structure modelrepositoryInitial release
1.1Jul 4, 2018Structure modelData collection / Database referencescitation_citation.journal_volume / _citation.page_first / _citation.page_last

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Assembly

Deposited unit
A: Polymerase acidic protein
B: RNA-directed RNA polymerase catalytic subunit
C: Polymerase basic protein 2
R: 3' promoter vRNA
V: 5' promoter vRNA


Theoretical massNumber of molelcules
Total (without water)264,5735
Polyers264,5735
Non-polymers00
Water0
1


TypeNameSymmetry operationNumber
identity operation1_5551
Buried area (Å2)40350
ΔGint (kcal/M)-245
Surface area (Å2)90220
MethodPISA

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Components

#1: Protein/peptide Polymerase acidic protein


Mass: 83232.109 Da / Num. of mol.: 1 / Source: (gene. exp.) Influenza B virus / Gene: PA / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: Q5V8Z9
#2: Protein/peptide RNA-directed RNA polymerase catalytic subunit


Mass: 84433.266 Da / Num. of mol.: 1 / Source: (gene. exp.) Influenza B virus / Gene: PB1 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: Q5V8Y6, RNA-directed RNA polymerase
#3: Protein/peptide Polymerase basic protein 2


Mass: 88028.211 Da / Num. of mol.: 1 / Source: (gene. exp.) Influenza B virus / Gene: PB2 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: Q5V8X3
#4: RNA chain 3' promoter vRNA


Mass: 4321.563 Da / Num. of mol.: 1 / Source: (synth.) Influenza B virus (B/Memphis/13/2003)
#5: RNA chain 5' promoter vRNA


Mass: 4557.820 Da / Num. of mol.: 1 / Source: (synth.) Influenza B virus (B/Memphis/13/2003)

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / Reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Influenza C virus polymerase bound to promoter vRNA / Type: COMPLEX / Entity ID: 1, 2, 3, 4, 5 / Source: RECOMBINANT
Molecular weightExperimental value: NO
Source (natural)Organism: Influenza B virus
Source (recombinant)Organism: Spodoptera frugiperda (fall armyworm)
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Tecnai Polara / Image courtesy: FEI Company
MicroscopyMicroscope model: FEI POLARA 300
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy
Image recordingElectron dose: 2.09 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k)

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Processing

EM software
IDNameVersionCategory
7UCSF Chimeramodel fitting
9RELIONvlioninitial Euler assignment
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
SymmetryPoint symmetry: C1
3D reconstructionResolution: 9.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number of particles: 9159 / Symmetry type: POINT
Atomic model buildingRef protocol: RIGID BODY FIT

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