SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AF, BF, CF, DF" IN EACH CHAIN ON SHEET ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AF, BF, CF, DF" IN EACH CHAIN ON SHEET RECORDS ARE ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL.
Mass: 18.015 Da / Num. of mol.: 4639 / Source method: isolated from a natural source / Formula: H2O
Compound details
INVOLVED IN THE CATABOLISM OF URACIL AND THYMIDINE LEADING TO THE FORMATION OF BETA-ALANINE. ...INVOLVED IN THE CATABOLISM OF URACIL AND THYMIDINE LEADING TO THE FORMATION OF BETA-ALANINE. CATALYSES THE REDUCTION OF URACIL AND THYMINE.
Sequence details
RESIDUE GLY 60 IS EXCHANGED FOR ASP, AS COMPARED TO THE SEQUENCE DEPOSITED IN SWISS-PROT
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.4 Å3/Da / Density % sol: 50 %
Crystal grow
pH: 4.7 Details: 100 MM SODIUM CITRATE PH 4.7, 16-20 % POLYETHYLENE GLYCOL 6000, 1 MM DTT
Crystal grow
*PLUS
Temperature: 20 ℃ / Method: vapor diffusion, hanging drop
Resolution: 1.9→30 Å / Num. obs: 326433 / % possible obs: 97.4 % / Redundancy: 3.3 % / Biso Wilson estimate: 9.1 Å2 / Rsym value: 0.067 / Net I/σ(I): 10.9
Reflection shell
Resolution: 1.9→1.97 Å / Redundancy: 1.6 % / Mean I/σ(I) obs: 3.9 / Rsym value: 0.24 / % possible all: 91.2
Reflection
*PLUS
Num. measured all: 2358836 / Rmerge(I) obs: 0.066
Reflection shell
*PLUS
% possible obs: 91.2 % / Rmerge(I) obs: 0.24
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Processing
Software
Name
Version
Classification
DENZO
datareduction
SCALEPACK
datascaling
SHARP
phasing
SOLVE
phasing
CNS
1
refinement
Refinement
Method to determine structure: OTHER / Resolution: 1.9→19.97 Å / Rfactor Rfree error: 0.002 / Data cutoff high absF: 4006295.34 / Isotropic thermal model: OVERALL / Cross valid method: THROUGHOUT / σ(F): 0 Details: THE C-TERMINAL RESIDUE WAS NOT SEEN IN THE DENSITY MAPS
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