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- PDB-6ehj: Human N-myristoyltransferase (NMT1) with Myristoyl-CoA and peptid... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6ehj | |||||||||
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Title | Human N-myristoyltransferase (NMT1) with Myristoyl-CoA and peptide bound | |||||||||
![]() | Glycylpeptide N-tetradecanoyltransferase 1 | |||||||||
![]() | TRANSFERASE / Myristoylation / complex / substrate peptide | |||||||||
Function / homology | ![]() myristoyltransferase activity / peptidyl-lysine N6-myristoyltransferase activity / N-terminal peptidyl-glycine N-myristoylation / Late Phase of HIV Life Cycle / cellular ketone metabolic process / regulation of opsin-mediated signaling pathway / Activation, myristolyation of BID and translocation to mitochondria / glycylpeptide N-tetradecanoyltransferase / glycylpeptide N-tetradecanoyltransferase activity / protein localization to membrane ...myristoyltransferase activity / peptidyl-lysine N6-myristoyltransferase activity / N-terminal peptidyl-glycine N-myristoylation / Late Phase of HIV Life Cycle / cellular ketone metabolic process / regulation of opsin-mediated signaling pathway / Activation, myristolyation of BID and translocation to mitochondria / glycylpeptide N-tetradecanoyltransferase / glycylpeptide N-tetradecanoyltransferase activity / protein localization to membrane / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups / Inactivation, recovery and regulation of the phototransduction cascade / in utero embryonic development / plasma membrane / cytoplasm / cytosol Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Perez-Dorado, I. / Ritzefeld, M. / Tate, E.W. | |||||||||
![]() | ![]() Title: High-resolution snapshots of human N-myristoyltransferase in action illuminate a mechanism promoting N-terminal Lys and Gly myristoylation. Authors: Dian, C. / Perez-Dorado, I. / Riviere, F. / Asensio, T. / Legrand, P. / Ritzefeld, M. / Shen, M. / Cota, E. / Meinnel, T. / Tate, E.W. / Giglione, C. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 183.8 KB | Display | ![]() |
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PDB format | ![]() | 140.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.3 MB | Display | ![]() |
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Full document | ![]() | 1.3 MB | Display | |
Data in XML | ![]() | 32.5 KB | Display | |
Data in CIF | ![]() | 45 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6qrmC ![]() 6sjzC ![]() 6sk2C ![]() 6sk3C ![]() 6sk8C ![]() 6skjC ![]() 4c2yS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 45453.348 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: NMT1 residues 109-114, 182-184, 316-317, 408-413, and 3 more residues belonging to the N-terminal tag ("GPH") were not modeled as they were flexible and thus not observed in the electron density map. Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: P30419, glycylpeptide N-tetradecanoyltransferase |
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-Non-polymers , 10 types, 262 molecules ![](data/chem/img/GOL.gif)
![](data/chem/img/MYA.gif)
![](data/chem/img/GLY.gif)
![](data/chem/img/SER.gif)
![](data/chem/img/ASN.gif)
![](data/chem/img/LYS.gif)
![](data/chem/img/PRO.gif)
![](data/chem/img/MYR.gif)
![](data/chem/img/COA.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/MYA.gif)
![](data/chem/img/GLY.gif)
![](data/chem/img/SER.gif)
![](data/chem/img/ASN.gif)
![](data/chem/img/LYS.gif)
![](data/chem/img/PRO.gif)
![](data/chem/img/MYR.gif)
![](data/chem/img/COA.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-GOL / #3: Chemical | ChemComp-MYA / | #4: Chemical | #5: Chemical | ChemComp-SER / #6: Chemical | #7: Chemical | ChemComp-LYS / #8: Chemical | #9: Chemical | #10: Chemical | #11: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 47.02 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 16-18% (v/w) PEG 4K, 5 mM NiCl2, 100 mM sodium citrate pH 4.5, and 2.5% (v/v) glycerol |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: May 18, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97625 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→47.082 Å / Num. obs: 49357 / % possible obs: 99.8 % / Redundancy: 6.7 % / Biso Wilson estimate: 32.87 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.112 / Rpim(I) all: 0.047 / Net I/σ(I): 12.3 |
Reflection shell | Resolution: 2.1→2.15 Å / Redundancy: 4.1 % / Rmerge(I) obs: 0.968 / Mean I/σ(I) obs: 1.3 / Num. unique obs: 3595 / CC1/2: 0.508 / Rpim(I) all: 0.047 / % possible all: 99.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4C2Y Resolution: 2.1→47.08 Å / SU ML: 0.29 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 25.26 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 100.29 Å2 / Biso mean: 37.5869 Å2 / Biso min: 14.94 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.1→47.08 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 18
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