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- PDB-6ech: Pyruvate Kinase Isoform L-type with phosphorylated Ser12 (pS12) i... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6ech | ||||||
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Title | Pyruvate Kinase Isoform L-type with phosphorylated Ser12 (pS12) in complex with FBP | ||||||
![]() | Pyruvate kinase PKLR | ||||||
![]() | TRANSFERASE / PKL / pyruvate kinase / Glycolysis | ||||||
Function / homology | ![]() Glycolysis / pyruvate biosynthetic process / ATP biosynthetic process / pyruvate kinase / pyruvate kinase activity / response to other organism / response to metal ion / monosaccharide binding / response to ATP / response to lithium ion ...Glycolysis / pyruvate biosynthetic process / ATP biosynthetic process / pyruvate kinase / pyruvate kinase activity / response to other organism / response to metal ion / monosaccharide binding / response to ATP / response to lithium ion / potassium ion binding / response to glucose / response to cAMP / cellular response to epinephrine stimulus / response to nutrient / glycolytic process / cellular response to insulin stimulus / kinase activity / response to heat / carbohydrate metabolic process / response to hypoxia / magnesium ion binding / ATP binding / plasma membrane / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
![]() | Padyana, A. / Tong, S. | ||||||
![]() | ![]() Title: Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production. Authors: Gassaway, B.M. / Cardone, R.L. / Padyana, A.K. / Petersen, M.C. / Judd, E.T. / Hayes, S. / Tong, S. / Barber, K.W. / Apostolidi, M. / Abulizi, A. / Sheetz, J.B. / Aerni, H.R. / Gross, S. / ...Authors: Gassaway, B.M. / Cardone, R.L. / Padyana, A.K. / Petersen, M.C. / Judd, E.T. / Hayes, S. / Tong, S. / Barber, K.W. / Apostolidi, M. / Abulizi, A. / Sheetz, J.B. / Aerni, H.R. / Gross, S. / Kung, C. / Samuel, V.T. / Shulman, G.I. / Kibbey, R.G. / Rinehart, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 447.4 KB | Display | ![]() |
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PDB format | ![]() | 358.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.9 MB | Display | ![]() |
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Full document | ![]() | 2.9 MB | Display | |
Data in XML | ![]() | 89.7 KB | Display | |
Data in CIF | ![]() | 128 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6eckC ![]() 4ip7S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
-Protein / Sugars , 2 types, 8 molecules ABCD![](data/chem/img/FBP.gif)
![](data/chem/img/FBP.gif)
#1: Protein | Mass: 59910.461 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Sugar | ChemComp-FBP / |
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-Non-polymers , 9 types, 1259 molecules ![](data/chem/img/MG.gif)
![](data/chem/img/ATP.gif)
![](data/chem/img/OXL.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/PG4.gif)
![](data/chem/img/PGE.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/ATP.gif)
![](data/chem/img/OXL.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/PG4.gif)
![](data/chem/img/PGE.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | ChemComp-MG / #4: Chemical | #5: Chemical | ChemComp-OXL / #6: Chemical | ChemComp-EDO / #7: Chemical | #8: Chemical | ChemComp-PG4 / | #9: Chemical | ChemComp-PGE / | #10: Chemical | ChemComp-CL / | #11: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.93 Å3/Da / Density % sol: 57.96 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 0.1 M HEPES, pH 7.5, 10% ethylene glycol, 8-12% PEG8000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Jun 14, 2018 | ||||||||||||||||||||||||||||||
Radiation | Monochromator: double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||
Reflection | Resolution: 2.19→49.52 Å / Num. obs: 144911 / % possible obs: 100 % / Redundancy: 6.7 % / CC1/2: 0.998 / Rmerge(I) obs: 0.085 / Rpim(I) all: 0.035 / Rrim(I) all: 0.092 / Net I/σ(I): 14.5 | ||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Phasing
Phasing | Method: ![]() |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 4IP7 Resolution: 2.19→49.52 Å / Cor.coef. Fo:Fc: 0.963 / Cor.coef. Fo:Fc free: 0.936 / SU B: 6.53 / SU ML: 0.156 / SU R Cruickshank DPI: 0.2071 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.207 / ESU R Free: 0.19 Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 144.69 Å2 / Biso mean: 45.771 Å2 / Biso min: 24.52 Å2
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Refinement step | Cycle: final / Resolution: 2.19→49.52 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.19→2.247 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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