+Open data
-Basic information
Entry | Database: PDB / ID: 6cpo | ||||||
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Title | Crystal structure of DR15 presenting the RQ13 peptide | ||||||
Components |
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Keywords | IMMUNE SYSTEM / IMMUNE RECEPTOR | ||||||
Function / homology | Function and homology information host cellular component / Synthesis And Processing Of GAG, GAGPOL Polyproteins / host cell nuclear membrane / regulation of interleukin-4 production / regulation of interleukin-10 production / positive regulation of T cell mediated immune response to tumor cell / myeloid dendritic cell antigen processing and presentation / antigen processing and presentation of endogenous peptide antigen via MHC class II / autolysosome membrane / regulation of T-helper cell differentiation ...host cellular component / Synthesis And Processing Of GAG, GAGPOL Polyproteins / host cell nuclear membrane / regulation of interleukin-4 production / regulation of interleukin-10 production / positive regulation of T cell mediated immune response to tumor cell / myeloid dendritic cell antigen processing and presentation / antigen processing and presentation of endogenous peptide antigen via MHC class II / autolysosome membrane / regulation of T-helper cell differentiation / positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation / MHC class II receptor activity / positive regulation of CD4-positive, alpha-beta T cell activation / antigen processing and presentation of peptide or polysaccharide antigen via MHC class II / positive regulation of memory T cell differentiation / positive regulation of monocyte differentiation / CD4 receptor binding / positive regulation of kinase activity / inflammatory response to antigenic stimulus / integrase activity / intermediate filament / Integration of viral DNA into host genomic DNA / Autointegration results in viral DNA circles / transport vesicle membrane / T-helper 1 type immune response / polysaccharide binding / Minus-strand DNA synthesis / Plus-strand DNA synthesis / Uncoating of the HIV Virion / 2-LTR circle formation / Translocation of ZAP-70 to Immunological synapse / Phosphorylation of CD3 and TCR zeta chains / viral budding via host ESCRT complex / Early Phase of HIV Life Cycle / Vpr-mediated nuclear import of PICs / positive regulation of insulin secretion involved in cellular response to glucose stimulus / Integration of provirus / APOBEC3G mediated resistance to HIV-1 infection / humoral immune response / macrophage differentiation / Generation of second messenger molecules / negative regulation of type II interferon production / immunological synapse / PD-1 signaling / epidermis development / Binding and entry of HIV virion / negative regulation of inflammatory response to antigenic stimulus / viral life cycle / negative regulation of T cell proliferation / Membrane binding and targetting of GAG proteins / MHC class II antigen presentation / detection of bacterium / T cell receptor binding / trans-Golgi network membrane / lumenal side of endoplasmic reticulum membrane / protein tetramerization / Assembly Of The HIV Virion / clathrin-coated endocytic vesicle membrane / HIV-1 retropepsin / : / ER to Golgi transport vesicle membrane / retroviral ribonuclease H / Budding and maturation of HIV virion / exoribonuclease H / : / exoribonuclease H activity / structural constituent of cytoskeleton / protein processing / cognition / host multivesicular body / positive regulation of T cell mediated cytotoxicity / peptide antigen assembly with MHC class II protein complex / MHC class II protein complex / RNA-directed DNA polymerase / peptide antigen binding / viral genome integration into host DNA / viral penetration into host nucleus / establishment of integrated proviral latency / endocytic vesicle membrane / antigen processing and presentation of exogenous peptide antigen via MHC class II / positive regulation of immune response / RNA-directed DNA polymerase activity / Interferon gamma signaling / positive regulation of T cell activation / Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases / RNA-DNA hybrid ribonuclease activity / Downstream TCR signaling / MHC class II protein complex binding / late endosome membrane / peptidase activity / T cell receptor signaling pathway / early endosome membrane / viral nucleocapsid / positive regulation of canonical NF-kappaB signal transduction / DNA recombination / positive regulation of MAPK cascade / adaptive immune response / positive regulation of viral entry into host cell / Hydrolases; Acting on ester bonds / lysosome Similarity search - Function | ||||||
Biological species | Homo sapiens (human) HIV-1 M:B_HXB2R (virus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.4 Å | ||||||
Authors | Farenc, C. / Gras, S. / Rossjohn, J. | ||||||
Citation | Journal: Sci Immunol / Year: 2018 Title: CD4+T cell-mediated HLA class II cross-restriction in HIV controllers. Authors: Galperin, M. / Farenc, C. / Mukhopadhyay, M. / Jayasinghe, D. / Decroos, A. / Benati, D. / Tan, L.L. / Ciacchi, L. / Reid, H.H. / Rossjohn, J. / Chakrabarti, L.A. / Gras, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6cpo.cif.gz | 174.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6cpo.ent.gz | 136.8 KB | Display | PDB format |
PDBx/mmJSON format | 6cpo.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cp/6cpo ftp://data.pdbj.org/pub/pdb/validation_reports/cp/6cpo | HTTPS FTP |
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-Related structure data
Related structure data | 6cphC 6cplC 6cpnC 6cqjC 6cqlC 6cqnC 6cqqC 6cqrC 4mdoS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
-Protein , 2 types, 4 molecules ADBE
#1: Protein | Mass: 21185.930 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: HLA-DRA, HLA-DRA1 / Production host: Homo sapiens (human) / References: UniProt: P01903 #2: Protein | Mass: 22073.605 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: HLA-DRB1 / Production host: Homo sapiens (human) / References: UniProt: D7RIH9, UniProt: P01911*PLUS |
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-Protein/peptide / Sugars , 2 types, 6 molecules CF
#3: Protein/peptide | Mass: 1600.797 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) HIV-1 M:B_HXB2R (virus) / References: UniProt: P04591, UniProt: P04585*PLUS #4: Sugar | ChemComp-NAG / |
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-Non-polymers , 3 types, 281 molecules
#5: Chemical | ChemComp-PGE / | ||
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#6: Chemical | #7: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.48 Å3/Da / Density % sol: 50.46 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / Details: PBS pH 7.5, Na acetate, PEG 4000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Australian Synchrotron / Beamline: MX1 / Wavelength: 0.9537 Å |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Apr 9, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9537 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→48.2 Å / Num. obs: 34562 / % possible obs: 100 % / Redundancy: 9 % / Biso Wilson estimate: 40.08 Å2 / Rpim(I) all: 0.076 / Net I/σ(I): 9 |
Reflection shell | Resolution: 2.4→2.49 Å / Num. unique obs: 3604 / Rpim(I) all: 0.408 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4MDO Resolution: 2.4→47.08 Å / Cor.coef. Fo:Fc: 0.92 / Cor.coef. Fo:Fc free: 0.887 / Rfactor Rfree error: 0 / SU R Cruickshank DPI: 0.442 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.438 / SU Rfree Blow DPI: 0.254 / SU Rfree Cruickshank DPI: 0.258
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Displacement parameters | Biso mean: 33.31 Å2
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Refine analyze | Luzzati coordinate error obs: 0.32 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: 1 / Resolution: 2.4→47.08 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.4→2.47 Å / Rfactor Rfree error: 0 / Total num. of bins used: 17
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