- PDB-6ckn: Crystal structure of an AF10 fragment -
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基本情報
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データベース: PDB / ID: 6ckn
タイトル
Crystal structure of an AF10 fragment
要素
Protein AF-10
キーワード
DNA BINDING PROTEIN / Structural Genomics / Structural Genomics Consortium / SGC
機能・相同性
機能・相同性情報
nucleosome binding / histone binding / chromatin binding / regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / protein-containing complex / DNA binding / nucleoplasm / nucleus / metal ion binding 類似検索 - 分子機能
温度: 291 K / 手法: 蒸気拡散法, シッティングドロップ法 詳細: This crystal's crystallization record is incomplete. We attempted to reconstruct the condition through reference to records from related crystals: 20% PEG-3350, 0.2 M magnesium nitrate. Additive: 1,6-hexanediol
構造決定の手法: 単波長異常分散 / 解像度: 2.49→39.32 Å / Cor.coef. Fo:Fc: 0.888 / Cor.coef. Fo:Fc free: 0.901 / Rfactor Rfree error: 0 / SU R Cruickshank DPI: 0.377 / 交差検証法: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.407 / SU Rfree Blow DPI: 0.257 / SU Rfree Cruickshank DPI: 0.253 詳細: Se-Met SAD phasing with shelx/phaser/parrot. Autotracing with BUCCANEER. Refinement with REFMAC and autobuster.