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Yorodumi- PDB-3bnq: Crystal Structure of the Homo sapiens Mitochondrial Ribosomal Dec... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3bnq | ||||||
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Title | Crystal Structure of the Homo sapiens Mitochondrial Ribosomal Decoding Site in the Presence of SrCl2 (A1555G mutant, Br-derivative) | ||||||
Components | (A site of human mitochondrial ribosome, ...) x 2 | ||||||
Keywords | RNA / ribosome / decoding site | ||||||
Function / homology | : / PAROMOMYCIN / STRONTIUM ION / RNA / RNA (> 10) Function and homology information | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MAD / Resolution: 2 Å | ||||||
Authors | Kondo, J. / Westhof, E. | ||||||
Citation | Journal: Nucleic Acids Res. / Year: 2008 Title: The bacterial and mitochondrial ribosomal A-site molecular switches possess different conformational substates Authors: Kondo, J. / Westhof, E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3bnq.cif.gz | 67.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3bnq.ent.gz | 48.9 KB | Display | PDB format |
PDBx/mmJSON format | 3bnq.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 3bnq_validation.pdf.gz | 787.6 KB | Display | wwPDB validaton report |
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Full document | 3bnq_full_validation.pdf.gz | 791.7 KB | Display | |
Data in XML | 3bnq_validation.xml.gz | 8.4 KB | Display | |
Data in CIF | 3bnq_validation.cif.gz | 11.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bn/3bnq ftp://data.pdbj.org/pub/pdb/validation_reports/bn/3bnq | HTTPS FTP |
-Related structure data
Related structure data | 3bnlC 3bnnC 3bnoC 3bnpC 3bnrC 3bnsC 3bntC C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Components on special symmetry positions |
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-Components
-A site of human mitochondrial ribosome, ... , 2 types, 4 molecules ABCD
#1: RNA chain | Mass: 7394.280 Da / Num. of mol.: 3 / Mutation: A1555G / Source method: obtained synthetically #2: RNA chain | | Mass: 6781.949 Da / Num. of mol.: 1 / Mutation: A1555G / Source method: obtained synthetically |
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-Non-polymers , 4 types, 144 molecules
#3: Chemical | ChemComp-SR / #4: Chemical | #5: Chemical | ChemComp-PAR / | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.33 Å3/Da / Density % sol: 47.2 % | ||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: Sodium cacodylate, KCl, spermine tetrachloride, paromomycin, 2-methyl-2,4-pentanediol, SrCl2, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||||||||||||||
Components of the solutions |
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-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID23-1 / Wavelength: 0.92070, 0.92110, 0.91625 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: May 20, 2007 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength |
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Reflection | Redundancy: 3.5 % / Av σ(I) over netI: 7 / Number: 60538 / Rmerge(I) obs: 0.049 / Rsym value: 0.049 / D res high: 2 Å / D res low: 49.662 Å / Num. obs: 17465 / % possible obs: 97.4 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Diffraction reflection shell |
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Reflection | Resolution: 2→49.791 Å / Num. obs: 17519 / % possible obs: 97.4 % / Redundancy: 3.5 % / Rmerge(I) obs: 0.05 / Rsym value: 0.05 / Net I/σ(I): 6.9 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell |
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-Phasing
Phasing | Method: MAD | ||||||||||||||||||||||||
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Phasing set |
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Phasing MAD set |
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-Processing
Software |
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Refinement | Method to determine structure: MAD / Resolution: 2→49.791 Å / FOM work R set: 0.795 / σ(F): 0
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Solvent computation | Bsol: 65.271 Å2 | ||||||||||||||||||||||||
Displacement parameters | Biso mean: 47.179 Å2
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Refinement step | Cycle: LAST / Resolution: 2→49.791 Å
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Refine LS restraints |
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Xplor file |
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