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- PDB-6bva: Ubiquitin Variant (UbV.Fl10.1) bound to a human Skp1-Fbl10 fragme... -

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Basic information

Entry
Database: PDB / ID: 6bva
TitleUbiquitin Variant (UbV.Fl10.1) bound to a human Skp1-Fbl10 fragment complex.
Components
  • Lysine-specific demethylase 2B
  • Polyubiquitin-B
  • S-phase kinase-associated protein 1
KeywordsPROTEIN BINDING / Ubiquitination
Function / homology
Function and homology information


initiation of neural tube closure / fourth ventricle development / midbrain-hindbrain boundary morphogenesis / third ventricle development / histone H3K36me/H3K36me2 demethylase activity / [histone H3]-dimethyl-L-lysine36 demethylase / embryonic camera-type eye morphogenesis / lateral ventricle development / negative regulation of neural precursor cell proliferation / F-box domain binding ...initiation of neural tube closure / fourth ventricle development / midbrain-hindbrain boundary morphogenesis / third ventricle development / histone H3K36me/H3K36me2 demethylase activity / [histone H3]-dimethyl-L-lysine36 demethylase / embryonic camera-type eye morphogenesis / lateral ventricle development / negative regulation of neural precursor cell proliferation / F-box domain binding / hindbrain development / PcG protein complex / unmethylated CpG binding / histone H3K36 demethylase activity / Cul7-RING ubiquitin ligase complex / positive regulation of ubiquitin protein ligase activity / Loss of Function of FBXW7 in Cancer and NOTCH1 Signaling / maintenance of protein location in nucleus / hypothalamus gonadotrophin-releasing hormone neuron development / female meiosis I / positive regulation of protein monoubiquitination / mitochondrion transport along microtubule / fat pad development / SCF ubiquitin ligase complex / ubiquitin ligase complex scaffold activity / midbrain development / SCF-dependent proteasomal ubiquitin-dependent protein catabolic process / positive regulation of stem cell population maintenance / female gonad development / seminiferous tubule development / Prolactin receptor signaling / male meiosis I / protein monoubiquitination / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator / cullin family protein binding / ubiquitin-like ligase-substrate adaptor activity / histone demethylase activity / protein K48-linked ubiquitination / Nuclear events stimulated by ALK signaling in cancer / energy homeostasis / regulation of neuron apoptotic process / regulation of proteasomal protein catabolic process / Maturation of protein E / Maturation of protein E / ER Quality Control Compartment (ERQC) / forebrain development / Myoclonic epilepsy of Lafora / FLT3 signaling by CBL mutants / Prevention of phagosomal-lysosomal fusion / IRAK2 mediated activation of TAK1 complex / Alpha-protein kinase 1 signaling pathway / Glycogen synthesis / IRAK1 recruits IKK complex / IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation / Membrane binding and targetting of GAG proteins / Endosomal Sorting Complex Required For Transport (ESCRT) / IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation / PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1 / Negative regulation of FLT3 / Constitutive Signaling by NOTCH1 HD Domain Mutants / Regulation of FZD by ubiquitination / TICAM1,TRAF6-dependent induction of TAK1 complex / NOTCH2 Activation and Transmission of Signal to the Nucleus / TICAM1-dependent activation of IRF3/IRF7 / APC/C:Cdc20 mediated degradation of Cyclin B / p75NTR recruits signalling complexes / Downregulation of ERBB4 signaling / APC-Cdc20 mediated degradation of Nek2A / PINK1-PRKN Mediated Mitophagy / TRAF6-mediated induction of TAK1 complex within TLR4 complex / TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling / Pexophagy / Regulation of innate immune responses to cytosolic DNA / InlA-mediated entry of Listeria monocytogenes into host cells / VLDLR internalisation and degradation / Downregulation of ERBB2:ERBB3 signaling / NF-kB is activated and signals survival / NRIF signals cell death from the nucleus / Regulation of PTEN localization / Activated NOTCH1 Transmits Signal to the Nucleus / Regulation of BACH1 activity / Translesion synthesis by REV1 / Synthesis of active ubiquitin: roles of E1 and E2 enzymes / Translesion synthesis by POLK / MAP3K8 (TPL2)-dependent MAPK1/3 activation / TICAM1, RIP1-mediated IKK complex recruitment / Downregulation of TGF-beta receptor signaling / Activation of IRF3, IRF7 mediated by TBK1, IKKε (IKBKE) / Translesion synthesis by POLI / Gap-filling DNA repair synthesis and ligation in GG-NER / Josephin domain DUBs / neuron projection morphogenesis / Regulation of activated PAK-2p34 by proteasome mediated degradation / InlB-mediated entry of Listeria monocytogenes into host cell / IKK complex recruitment mediated by RIP1 / JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 / TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) / regulation of mitochondrial membrane potential / N-glycan trimming in the ER and Calnexin/Calreticulin cycle / Autodegradation of Cdh1 by Cdh1:APC/C
Similarity search - Function
PHD-finger / Jumonji, helical domain / Jumonji helical domain / Leucine-rich repeat, cysteine-containing subtype / Leucine-rich repeat - CC (cysteine-containing) subfamily / Cupin-like domain 8 / Cupin-like domain / F-box-like / SKP1 component, dimerisation / S-phase kinase-associated protein 1 ...PHD-finger / Jumonji, helical domain / Jumonji helical domain / Leucine-rich repeat, cysteine-containing subtype / Leucine-rich repeat - CC (cysteine-containing) subfamily / Cupin-like domain 8 / Cupin-like domain / F-box-like / SKP1 component, dimerisation / S-phase kinase-associated protein 1 / SKP1-like, dimerisation domain superfamily / Skp1 family, dimerisation domain / CXXC zinc finger domain / Zinc finger, CXXC-type / Zinc finger CXXC-type profile. / F-box domain / Potassium Channel Kv1.1; Chain A / Potassium Channel Kv1.1; Chain A / S-phase kinase-associated protein 1-like / SKP1 component, POZ domain / Skp1 family, tetramerisation domain / Found in Skp1 protein family / A domain family that is part of the cupin metalloenzyme superfamily. / JmjC domain / JmjC domain profile. / SKP1/BTB/POZ domain superfamily / Zinc finger PHD-type profile. / Zinc finger, PHD-finger / Leucine-rich repeat domain superfamily / Zinc finger, PHD-type / PHD zinc finger / Ubiquitin domain signature. / Ubiquitin conserved site / Ubiquitin domain / Zinc finger, FYVE/PHD-type / Ubiquitin family / Ubiquitin homologues / Ubiquitin domain profile. / Ubiquitin-like domain / Zinc finger, RING/FYVE/PHD-type / Ubiquitin-like domain superfamily / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
Polyubiquitin-B / S-phase kinase-associated protein 1 / Lysine-specific demethylase 2B
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.66 Å
AuthorsManczyk, N. / Sicheri, F.
Funding support Canada, 1items
OrganizationGrant numberCountry
Canadian Institutes of Health Research (CIHR)FDN 143277 Canada
CitationJournal: Structure / Year: 2018
Title: A Structure-Based Strategy for Engineering Selective Ubiquitin Variant Inhibitors of Skp1-Cul1-F-Box Ubiquitin Ligases.
Authors: Gorelik, M. / Manczyk, N. / Pavlenco, A. / Kurinov, I. / Sidhu, S.S. / Sicheri, F.
History
DepositionDec 12, 2017Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jul 18, 2018Provider: repository / Type: Initial release
Revision 1.1Aug 8, 2018Group: Data collection / Database references / Derived calculations
Category: citation / citation_author ...citation / citation_author / pdbx_struct_assembly_gen / pdbx_struct_assembly_prop
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.name / _pdbx_struct_assembly_gen.asym_id_list / _pdbx_struct_assembly_prop.value
Revision 1.2Sep 19, 2018Group: Data collection / Database references / Category: citation / Item: _citation.journal_volume / _citation.page_first
Revision 1.3Jan 8, 2020Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.4Oct 4, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
D: S-phase kinase-associated protein 1
E: Lysine-specific demethylase 2B
B: Polyubiquitin-B
A: Polyubiquitin-B
C: S-phase kinase-associated protein 1
F: Lysine-specific demethylase 2B


Theoretical massNumber of molelcules
Total (without water)68,9236
Polymers68,9236
Non-polymers00
Water72140
1
D: S-phase kinase-associated protein 1
E: Lysine-specific demethylase 2B
B: Polyubiquitin-B


Theoretical massNumber of molelcules
Total (without water)34,4613
Polymers34,4613
Non-polymers00
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3740 Å2
ΔGint-35 kcal/mol
Surface area13720 Å2
MethodPISA
2
A: Polyubiquitin-B
C: S-phase kinase-associated protein 1
F: Lysine-specific demethylase 2B


Theoretical massNumber of molelcules
Total (without water)34,4613
Polymers34,4613
Non-polymers00
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4170 Å2
ΔGint-37 kcal/mol
Surface area13860 Å2
MethodPISA
Unit cell
Length a, b, c (Å)65.360, 81.793, 128.685
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

#1: Protein S-phase kinase-associated protein 1 / Cyclin-A/CDK2-associated protein p19 / p19A / Organ of Corti protein 2 / OCP-2 / Organ of Corti ...Cyclin-A/CDK2-associated protein p19 / p19A / Organ of Corti protein 2 / OCP-2 / Organ of Corti protein II / OCP-II / RNA polymerase II elongation factor-like protein / SIII / Transcription elongation factor B polypeptide 1-like / p19skp1


Mass: 18808.096 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: SKP1, EMC19, OCP2, SKP1A, TCEB1L / Production host: Escherichia coli (E. coli) / References: UniProt: P63208
#2: Protein/peptide Lysine-specific demethylase 2B / CXXC-type zinc finger protein 2 / F-box and leucine-rich repeat protein 10 / F-box protein FBL10 / ...CXXC-type zinc finger protein 2 / F-box and leucine-rich repeat protein 10 / F-box protein FBL10 / F-box/LRR-repeat protein 10 / JmjC domain-containing histone demethylation protein 1B / Jumonji domain-containing EMSY-interactor methyltransferase motif protein / Protein JEMMA / Protein-containing CXXC domain 2 / [Histone-H3]-lysine-36 demethylase 1B


Mass: 5680.714 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: KDM2B, CXXC2, FBL10, FBXL10, JHDM1B, PCCX2 / Production host: Escherichia coli (E. coli)
References: UniProt: Q8NHM5, [histone H3]-dimethyl-L-lysine36 demethylase
#3: Protein Polyubiquitin-B


Mass: 9972.468 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: UBB / Production host: Escherichia coli (E. coli) / References: UniProt: P0CG47
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 40 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.5 Å3/Da / Density % sol: 50.71 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5
Details: 0.1 M Hepes pH 7.5, 0.2 M calcium chloride, 18% (w/v) PEG 6000

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Data collection

DiffractionMean temperature: 93.15 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.9791 Å
DetectorType: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Nov 21, 2016
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9791 Å / Relative weight: 1
ReflectionResolution: 2.66→50 Å / Num. obs: 20716 / % possible obs: 99.8 % / Redundancy: 5.3 % / Rsym value: 0.174 / Net I/σ(I): 16.5
Reflection shellResolution: 2.66→2.76 Å / Redundancy: 4.6 % / Mean I/σ(I) obs: 2.2 / Rsym value: 1.067 / % possible all: 99.9

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Processing

Software
NameVersionClassification
PHENIX(1.12_2829: ???)refinement
HKL-2000data reduction
HKL-2000data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1UBQ, 5IBK, 5JH5

1ubq

5ibk

5jh5


Resolution: 2.66→47.46 Å / SU ML: 0.35 / Cross valid method: FREE R-VALUE / σ(F): 2.01 / Phase error: 28.43 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.263 1060 5.19 %
Rwork0.216 --
obs0.218 20406 99.7 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 2.66→47.46 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4071 0 0 40 4111
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0064147
X-RAY DIFFRACTIONf_angle_d0.8815661
X-RAY DIFFRACTIONf_dihedral_angle_d8.4112498
X-RAY DIFFRACTIONf_chiral_restr0.062687
X-RAY DIFFRACTIONf_plane_restr0.006719
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.66-2.78110.3161260.25032366X-RAY DIFFRACTION100
2.7811-2.92770.28691480.24482373X-RAY DIFFRACTION100
2.9277-3.11110.31921360.24022377X-RAY DIFFRACTION100
3.1111-3.35120.30331270.2492393X-RAY DIFFRACTION100
3.3512-3.68830.27441120.20952417X-RAY DIFFRACTION100
3.6883-4.22180.22051160.19022421X-RAY DIFFRACTION100
4.2218-5.31780.24361590.18642434X-RAY DIFFRACTION100
5.3178-47.46820.25711360.22972565X-RAY DIFFRACTION100
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.0754-1.5272-1.61047.76536.68338.8378-0.6785-0.0362-0.31460.63571.5047-1.2133-0.17521.6481-0.39780.2402-0.0559-0.0470.45560.08070.723826.977816.7573-0.9584
26.56513.8832-3.66573.97450.51236.0485-0.14750.9105-0.58790.3746-0.2796-0.96380.949-0.01470.24570.24330.0461-0.07070.47050.03120.577923.738311.23441.4948
33.5368-0.6777-4.74292.84010.03019.04610.07221.4712-0.312-1.11711.0812-0.6183-0.7563-0.6841-0.69690.4622-0.02310.06720.5628-0.13240.620614.108615.38830.7426
42.13671.0159-3.32556.6833-4.45478.1506-0.21170.27130.8898-0.20460.24370.89360.7586-1.0221-0.32550.4278-0.06340.01740.6123-0.04840.676420.084320.8487-0.7975
59.91513.6918-4.3947.5106-5.46157.4265-0.2685-0.75670.2164-0.42480.1011-0.38590.63940.91340.2920.3810.04910.00410.4522-0.08410.399426.188413.611411.0728
65.4413-6.49122.45537.94-2.49822.0558-1.1497-0.2442-1.7003-0.71570.81411.51161.0926-0.8470.43220.7649-0.08120.32950.4796-0.13232.000213.01435.813611.5749
70.8863-1.0157-1.71542.81221.72177.1872-0.5477-1.599-2.00091.8760.20911.76221.2817-0.54690.28810.90730.01460.2910.95970.30390.93612.54768.486726.1352
87.6782-2.5712-0.00215.7799-0.19352.241-0.0085-1.46160.3130.98940.13330.14180.53580.65850.22970.62810.0707-0.06160.781-0.15250.412422.74317.604818.5589
90.1212-0.0539-0.06064.74515.10376.2805-0.7492-1.91570.51841.35890.335-0.10890.6457-0.0082-0.27460.6890.2243-0.12921.2016-0.32890.476417.307918.769726.8367
101.0247-0.79120.34442.33880.06890.15330.0832-0.6090.63110.26720.5194-0.05960.2571.0081-0.28120.68270.27210.17231.1222-0.58471.020718.829133.377434.4118
110.7739-0.3039-0.15970.11720.06040.0206-0.2229-0.4213-0.32510.18860.15960.3756-0.6294-0.40650.44671.81610.30161.04642.573-0.66370.98455.132329.990143.7777
124.289-1.9422-0.0336.07121.9389.9543-1.04130.05750.1095-0.13310.27550.1462-1.1011-1.30690.20610.63360.185-0.03211.0437-0.3710.480411.712125.07923.7655
135.0518-5.13113.28435.7676-2.30534.0197-0.3618-4.22320.28411.09671.5133-0.3999-0.3466-0.0981-1.06431.67170.31160.16252.002-0.15071.01188.4821.940936.0296
140.75330.4779-0.25390.41630.16020.94680.2496-0.29680.15680.35380.3460.1676-0.35430.16810.85211.76750.6018-0.26271.5196-0.96170.60848.912531.22131.0849
154.98711.24322.11871.83312.97794.83220.20830.0340.05730.53650.52310.36630.2975-1.2706-0.0621.16940.04690.24141.5513-0.21970.66970.696522.295529.3717
166.2129-2.76385.18818.0761-4.05897.0228-0.46-0.06880.3220.04430.910.2901-0.713-0.4731-0.57880.36680.0672-0.01170.6056-0.04660.53994.625526.3851.8427
177.09643.8001-6.2717.9685-3.99786.3604-0.96291.43442.2427-1.12731.06670.1742-0.358-1.5667-0.25830.6154-0.038-0.06210.47510.08110.87227.305538.5865-2.3313
188.6416-4.3547-1.41125.47721.10257.3829-0.11150.43451.84970.14330.2671-0.696-0.35780.6989-0.34660.3164-0.083-0.02870.8368-0.03720.97513.179431.098-3.194
196.6668-1.4272-2.28895.34453.61585.7481-0.8757-1.46870.99760.39670.7054-1.0918-0.6130.1702-0.51980.7405-0.022-0.3883-0.0086-0.24580.611757.352931.44571.8366
209.00753.16427.06276.63275.06697.98080.21821.3105-0.8733-0.7332-0.0368-0.7544-1.0450.8329-0.42420.3703-0.11630.05040.6287-0.02570.373254.655821.628154.4022
219.67052.89224.90392.17071.89422.6164-0.07730.35591.3737-0.3229-0.0859-0.5884-0.8115-0.70050.2180.52690.0118-0.0690.32940.01070.572559.002732.393768.4365
226.3047-1.6756-2.16886.89290.44248.13910.046-0.62562.1059-0.2544-0.02940.1439-0.8820.74870.18240.4509-0.1623-0.03970.3167-0.05460.714752.821838.697566.2797
237.46234.1221-0.31497.65913.77343.9442-0.55640.24681.5803-1.30060.01180.3454-0.5355-0.23830.39870.3001-0.0105-0.02480.44530.18020.512448.727533.172858.8906
249.42783.63623.08783.47435.00088.00240.02830.51751.1957-0.4463-0.41861.0661-0.0591-0.93620.15390.3544-0.0511-0.11850.5341-0.02590.710243.094231.387970.2362
255.24030.31390.83361.34041.35814.97180.169-0.71050.46510.0763-0.56870.3331-0.3451-1.22930.4060.2820.00040.01470.4324-0.12920.432148.405629.938367.8853
267.2161-3.30753.20254.1981-4.29194.5184-0.0163-0.16010.1106-0.29910.3892-0.26740.0413-0.9542-0.32780.28950.07850.03220.3551-0.07630.407234.445517.055965.2144
273.7724-0.7071-0.25615.91911.83943.9548-0.0514-0.37780.07290.10780.0734-0.25240.2780.27680.01190.24160.0230.03870.42970.03080.429839.194514.849660.8596
286.05923.6332-5.29733.4647-1.55076.9374-0.71380.6989-2.1605-0.4926-0.075-0.70950.9310.38170.56850.5061-0.01060.09640.5348-0.18180.546342.55287.922945.2201
290.81490.6945-2.14983.5599-2.95886.0508-0.15580.80440.0797-0.37540.2284-0.1609-0.0111-0.16970.07730.4253-0.10880.02590.83850.13590.275344.624422.915335.4545
307.02360.8787-0.01445.7255-3.296.1735-0.33760.5115-0.6744-0.8107-0.1695-0.3548-0.52560.40570.56850.7207-0.02870.10570.95840.05810.389850.01323.329934.8169
313.94311.1673-2.40698.1701-2.95863.66350.0916-0.434-0.3121-0.32310.2419-1.0416-0.4081.42360.31510.7023-0.13210.10231.20880.16280.39554.098327.698333.9802
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1CHAIN 'D' AND (RESID 0 THROUGH 12 )
2X-RAY DIFFRACTION2CHAIN 'D' AND (RESID 13 THROUGH 23 )
3X-RAY DIFFRACTION3CHAIN 'D' AND (RESID 24 THROUGH 31 )
4X-RAY DIFFRACTION4CHAIN 'D' AND (RESID 32 THROUGH 51 )
5X-RAY DIFFRACTION5CHAIN 'D' AND (RESID 52 THROUGH 64 )
6X-RAY DIFFRACTION6CHAIN 'D' AND (RESID 65 THROUGH 74 )
7X-RAY DIFFRACTION7CHAIN 'D' AND (RESID 75 THROUGH 86 )
8X-RAY DIFFRACTION8CHAIN 'D' AND (RESID 87 THROUGH 110 )
9X-RAY DIFFRACTION9CHAIN 'D' AND (RESID 111 THROUGH 138 )
10X-RAY DIFFRACTION10CHAIN 'D' AND (RESID 139 THROUGH 147 )
11X-RAY DIFFRACTION11CHAIN 'D' AND (RESID 148 THROUGH 161 )
12X-RAY DIFFRACTION12CHAIN 'E' AND (RESID 1064 THROUGH 1076 )
13X-RAY DIFFRACTION13CHAIN 'E' AND (RESID 1077 THROUGH 1087 )
14X-RAY DIFFRACTION14CHAIN 'E' AND (RESID 1088 THROUGH 1097 )
15X-RAY DIFFRACTION15CHAIN 'E' AND (RESID 1098 THROUGH 1103 )
16X-RAY DIFFRACTION16CHAIN 'B' AND (RESID 0 THROUGH 24 )
17X-RAY DIFFRACTION17CHAIN 'B' AND (RESID 25 THROUGH 41 )
18X-RAY DIFFRACTION18CHAIN 'B' AND (RESID 42 THROUGH 80 )
19X-RAY DIFFRACTION19CHAIN 'A' AND (RESID -2 THROUGH 7 )
20X-RAY DIFFRACTION20CHAIN 'A' AND (RESID 8 THROUGH 18 )
21X-RAY DIFFRACTION21CHAIN 'A' AND (RESID 19 THROUGH 24 )
22X-RAY DIFFRACTION22CHAIN 'A' AND (RESID 25 THROUGH 41 )
23X-RAY DIFFRACTION23CHAIN 'A' AND (RESID 42 THROUGH 51 )
24X-RAY DIFFRACTION24CHAIN 'A' AND (RESID 52 THROUGH 63 )
25X-RAY DIFFRACTION25CHAIN 'A' AND (RESID 64 THROUGH 83 )
26X-RAY DIFFRACTION26CHAIN 'C' AND (RESID 1 THROUGH 12 )
27X-RAY DIFFRACTION27CHAIN 'C' AND (RESID 13 THROUGH 64 )
28X-RAY DIFFRACTION28CHAIN 'C' AND (RESID 65 THROUGH 96 )
29X-RAY DIFFRACTION29CHAIN 'C' AND (RESID 97 THROUGH 161 )
30X-RAY DIFFRACTION30CHAIN 'F' AND (RESID 1064 THROUGH 1087 )
31X-RAY DIFFRACTION31CHAIN 'F' AND (RESID 1088 THROUGH 1103 )

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