Journal: PLoS Pathog. / Year: 2018 Title: Cryo-EM structure of the SARS coronavirus spike glycoprotein in complex with its host cell receptor ACE2. Authors: Wenfei Song / Miao Gui / Xinquan Wang / Ye Xiang
Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Vitrification
Cryogen name: ETHANE
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Electron microscopy imaging
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
Microscopy
Microscope model: FEI TITAN KRIOS
Electron gun
Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 / Illumination mode: FLOOD BEAM
Electron lens
Mode: BRIGHT FIELDBright-field microscopy
Image recording
Average exposure time: 8 / Electron dose: 50 / Film or detector model: GATAN K2 SUMMIT (4k x 4k)
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Processing
EM software
Name: RELION / Version: 1.4 / Category: 3D reconstruction
CTF correction
Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Symmetry
Point symmetry: C1
3D reconstruction
Resolution: 3.9 / Resolution method: FSC 0.143 CUT-OFF / Number of particles: 174489 / Symmetry type: POINT
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