|Entry||Database: EMDB / ID: 9584|
|Title||Trypsin-cleaved SARS-CoV spike glycoprotein and ACE2 complex, ACE2-free conformation with one RBD in up position|
|Sample||Trypsin-cleaved SARS-CoV spike glycoprotein:|
|Method||single particle reconstruction / cryo EM / 9 Å resolution|
|Authors||Gui M / Song W|
|Citation||Journal: PLoS Pathog. / Year: 2018|
Title: Cryo-EM structure of the SARS coronavirus spike glycoprotein in complex with its host cell receptor ACE2.
Authors: Wenfei Song / Miao Gui / Xinquan Wang / Ye Xiang
|Date||Deposition: Jul 25, 2018 / Header (metadata) release: Aug 8, 2018 / Map release: Aug 8, 2018 / Last update: Aug 22, 2018|
|Structure viewer||EM map: |
Downloads & links
|File||emd_9584.map.gz (map file in CCP4 format, 7627 KB)|
|Projections & slices|
Images are generated by Spider.
|Voxel size||X=Y=Z: 2.54 Å|
CCP4 map header:
-Entire Trypsin-cleaved SARS-CoV spike glycoprotein
|Entire||Name: Trypsin-cleaved SARS-CoV spike glycoprotein / Number of components: 1|
-Component #1: protein, Trypsin-cleaved SARS-CoV spike glycoprotein
|Protein||Name: Trypsin-cleaved SARS-CoV spike glycoprotein / Recombinant expression: No|
|Mass||Theoretical: 400 kDa|
|Source||Species: SARS coronavirus|
|Source (engineered)||Expression System: Spodoptera frugiperda (fall armyworm)|
|Specimen||Specimen state: particle / Method: cryo EM|
|Sample solution||pH: 7.2|
|Vitrification||Cryogen name: ETHANE|
-Electron microscopy imaging
Model: Titan Krios / Image courtesy: FEI Company
|Imaging||Microscope: FEI TITAN KRIOS|
|Electron gun||Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 5 e/Å2 / Illumination mode: FLOOD BEAM|
|Lens||Imaging mode: BRIGHT FIELD|
|Specimen Holder||Model: OTHER|
|Camera||Detector: GATAN K2 SUMMIT (4k x 4k)|
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