+
Open data
-
Basic information
Entry | Database: PDB / ID: 6a8w | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal structure of the FHA domain of Far9 | ||||||
![]() | Vacuolar protein sorting-associated protein 64 | ||||||
![]() | PROTEIN BINDING / FHA domain / phosphopeptide recognition / homolog of SLMAP | ||||||
Function / homology | ![]() re-entry into mitotic cell cycle after pheromone arrest / protein targeting to vacuole / endoplasmic reticulum-Golgi intermediate compartment / TOR signaling / membrane => GO:0016020 / endoplasmic reticulum membrane / endoplasmic reticulum / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Chen, M. / Zhang, W.Q. / Zhou, Z.C. | ||||||
![]() | ![]() Title: Topological Structure and Dynamic Assembly of the STRIPAK Complex Authors: Chen, M. / Zhou, Z.C. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 37.9 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 27.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 424.2 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 424.2 KB | Display | |
Data in XML | ![]() | 8.2 KB | Display | |
Data in CIF | ![]() | 11 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-
Links
-
Assembly
Deposited unit | ![]()
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
Unit cell |
| |||||||||
Components on special symmetry positions |
|
-
Components
#1: Protein | Mass: 15677.396 Da / Num. of mol.: 1 / Fragment: UNP residues 160-295 / Mutation: L167M, T269M Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: ATCC 204508 / S288c / Gene: VPS64, FAR9, YDR200C, YD9346.10C / Production host: ![]() ![]() |
---|---|
#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 1.97 Å3/Da / Density % sol: 37.43 % |
---|---|
Crystal grow | Temperature: 289 K / Method: vapor diffusion, sitting drop Details: 6%(v/v) Tacsimate pH 6.0; 0.1M MES pH 6.0; 25%(w/v) polyethylene glycol 4,000. |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Oct 11, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9792 Å / Relative weight: 1 |
Reflection | Resolution: 1.85→50 Å / Num. obs: 11148 / % possible obs: 99.6 % / Redundancy: 13.2 % / Biso Wilson estimate: 19.42 Å2 / Rmerge(I) obs: 0.186 / Rpim(I) all: 0.053 / Rrim(I) all: 0.193 / Net I/σ(I): 19.6 |
Reflection shell | Resolution: 1.85→1.88 Å / Redundancy: 11.1 % / Mean I/σ(I) obs: 2.4 / Num. unique obs: 550 / CC1/2: 0.759 / Rpim(I) all: 0.403 / % possible all: 98.7 |
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Details: SF FILE CONTAINS FRIEDEL PAIRS UNDER I_MINUS AND I_PLUS COLUMNS.
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.844→29.707 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell |
|