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- PDB-5udc: Crystal Structure of RSV F A2 Bound to MEDI8897 -

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Basic information

Entry
Database: PDB / ID: 5udc
TitleCrystal Structure of RSV F A2 Bound to MEDI8897
Components
  • Fusion glycoprotein F0
  • MEDI8897 Fab Heavy Chain
  • MEDI8897 Fab Light Chain
KeywordsViral Protein/Immune System / immune system / antibody / fusion glycoprotein / virus / Viral Protein-Immune System complex
Function / homology
Function and homology information


positive regulation of syncytium formation by virus / Translation of respiratory syncytial virus mRNAs / RSV-host interactions / Maturation of hRSV A proteins / Assembly and release of respiratory syncytial virus (RSV) virions / host cell Golgi membrane / Respiratory syncytial virus (RSV) attachment and entry / entry receptor-mediated virion attachment to host cell / symbiont entry into host cell / fusion of virus membrane with host plasma membrane ...positive regulation of syncytium formation by virus / Translation of respiratory syncytial virus mRNAs / RSV-host interactions / Maturation of hRSV A proteins / Assembly and release of respiratory syncytial virus (RSV) virions / host cell Golgi membrane / Respiratory syncytial virus (RSV) attachment and entry / entry receptor-mediated virion attachment to host cell / symbiont entry into host cell / fusion of virus membrane with host plasma membrane / viral envelope / host cell plasma membrane / virion membrane / identical protein binding / plasma membrane
Similarity search - Function
Precursor fusion glycoprotein F0, Paramyxoviridae / Fusion glycoprotein F0 / Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta
Similarity search - Domain/homology
Fusion glycoprotein F0
Similarity search - Component
Biological speciesHomo sapiens (human)
Human respiratory syncytial virus A
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 3.45 Å
AuthorsMcLellan, J.S.
CitationJournal: Sci Transl Med / Year: 2017
Title: A highly potent extended half-life antibody as a potential RSV vaccine surrogate for all infants.
Authors: Zhu, Q. / McLellan, J.S. / Kallewaard, N.L. / Ulbrandt, N.D. / Palaszynski, S. / Zhang, J. / Moldt, B. / Khan, A. / Svabek, C. / McAuliffe, J.M. / Wrapp, D. / Patel, N.K. / Cook, K.E. / ...Authors: Zhu, Q. / McLellan, J.S. / Kallewaard, N.L. / Ulbrandt, N.D. / Palaszynski, S. / Zhang, J. / Moldt, B. / Khan, A. / Svabek, C. / McAuliffe, J.M. / Wrapp, D. / Patel, N.K. / Cook, K.E. / Richter, B.W.M. / Ryan, P.C. / Yuan, A.Q. / Suzich, J.A.
History
DepositionDec 26, 2016Deposition site: RCSB / Processing site: RCSB
Revision 1.0May 17, 2017Provider: repository / Type: Initial release
Revision 2.0Jul 29, 2020Group: Atomic model / Data collection ...Atomic model / Data collection / Derived calculations / Structure summary
Category: atom_site / chem_comp ...atom_site / chem_comp / entity / pdbx_branch_scheme / pdbx_chem_comp_identifier / pdbx_entity_branch / pdbx_entity_branch_descriptor / pdbx_entity_branch_link / pdbx_entity_branch_list / pdbx_entity_nonpoly / pdbx_nonpoly_scheme / pdbx_struct_assembly_gen / struct_asym / struct_conn / struct_site / struct_site_gen
Item: _atom_site.auth_asym_id / _atom_site.auth_seq_id ..._atom_site.auth_asym_id / _atom_site.auth_seq_id / _atom_site.label_asym_id / _atom_site.label_entity_id / _chem_comp.name / _chem_comp.type / _pdbx_struct_assembly_gen.asym_id_list / _struct_conn.pdbx_role / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id
Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 2.1Oct 4, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description / Structure summary
Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_conn / struct_ncs_dom_lim
Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI ..._chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.pdbx_leaving_atom_flag / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
H: MEDI8897 Fab Heavy Chain
L: MEDI8897 Fab Light Chain
F: Fusion glycoprotein F0
B: MEDI8897 Fab Heavy Chain
C: MEDI8897 Fab Light Chain
A: Fusion glycoprotein F0
E: MEDI8897 Fab Heavy Chain
G: MEDI8897 Fab Light Chain
D: Fusion glycoprotein F0
hetero molecules


Theoretical massNumber of molelcules
Total (without water)334,05812
Polymers332,1049
Non-polymers1,9553
Water00
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area31730 Å2
ΔGint-123 kcal/mol
Surface area105660 Å2
MethodPISA
Unit cell
Length a, b, c (Å)103.780, 126.310, 162.310
Angle α, β, γ (deg.)90.000, 102.560, 90.000
Int Tables number4
Space group name H-MP1211
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and (resid 27 through 97 or resid 137 through 512))
21(chain D and resid 27 through 512)
31(chain F and (resid 27 through 97 or resid 137 through 512))
12chain C
22(chain G and resid 1 through 211)
32chain L
13(chain B and (resid 2 through 126 or resid 135 through 211))
23(chain E and resid 2 through 211)
33(chain H and (resid 2 through 126 or resid 135 through 211))

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111ASNASNMETMET(chain A and (resid 27 through 97 or resid 137 through 512))AF27 - 9727 - 97
121PHEPHELEULEU(chain A and (resid 27 through 97 or resid 137 through 512))AF137 - 512137 - 512
211ASNASNLEULEU(chain D and resid 27 through 512)DI27 - 51227 - 512
311ASNASNMETMET(chain F and (resid 27 through 97 or resid 137 through 512))FC27 - 9727 - 97
321PHEPHELEULEU(chain F and (resid 27 through 97 or resid 137 through 512))FC137 - 512137 - 512
112ASPASPARGARGchain CCE1 - 2111 - 211
212ASPASPARGARG(chain G and resid 1 through 211)GH1 - 2111 - 211
312ASPASPARGARGchain LLB1 - 2111 - 211
113VALVALPROPRO(chain B and (resid 2 through 126 or resid 135 through 211))BD2 - 1262 - 139
123THRTHRVALVAL(chain B and (resid 2 through 126 or resid 135 through 211))BD135 - 211148 - 224
213VALVALVALVAL(chain E and resid 2 through 211)EG2 - 2112 - 224
313VALVALPROPRO(chain H and (resid 2 through 126 or resid 135 through 211))HA2 - 1262 - 139
323THRTHRVALVAL(chain H and (resid 2 through 126 or resid 135 through 211))HA135 - 211148 - 224

NCS ensembles :
ID
1
2
3

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Components

#1: Antibody MEDI8897 Fab Heavy Chain


Mass: 24157.061 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): 293F / Production host: Homo sapiens (human)
#2: Antibody MEDI8897 Fab Light Chain


Mass: 23325.865 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): 293F / Production host: Homo sapiens (human)
#3: Protein Fusion glycoprotein F0 / Protein F


Mass: 63218.344 Da / Num. of mol.: 3 / Mutation: S155C, S290C, S190F, V207L
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Human respiratory syncytial virus A (strain A2)
Strain: A2 / Plasmid: paH / Cell (production host): 293F / Production host: Homo sapiens (human) / References: UniProt: P03420
#4: Polysaccharide alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D- ...alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 1883.668 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-2DManpa1-2DManpa1-3[DManpa1-2DManpa1-3[DManpa1-2DManpa1-6]DManpa1-6]DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/3,11,10/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3-3-3-3-3-3-3-3/a4-b1_b4-c1_c3-d1_c6-g1_d2-e1_e2-f1_g3-h1_g6-j1_h2-i1_j2-k1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{[(2+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}}[(6+1)][a-D-Manp]{[(3+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}[(6+1)][a-D-Manp]{[(2+1)][a-D-Manp]{}}}}}}}LINUCSPDB-CARE
#5: Chemical ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Cl

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.38 Å3/Da / Density % sol: 63.65 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8.5
Details: 10% w/v PEG8000, 20% v/v ethylene glycol, 0.1 M Tris/BICINE pH 8.5, 0.02 M carboxylic acids, 0.3 M NDSB-195

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.97918 Å
DetectorType: ADSC QUANTUM 315 / Detector: CCD / Date: Jul 19, 2014
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97918 Å / Relative weight: 1
ReflectionResolution: 3.45→43.14 Å / Num. obs: 53451 / % possible obs: 99 % / Redundancy: 3.1 % / Biso Wilson estimate: 111.68 Å2 / CC1/2: 0.996 / Rmerge(I) obs: 0.085 / Rpim(I) all: 0.056 / Rrim(I) all: 0.102 / Net I/σ(I): 8 / Num. measured all: 165678 / Scaling rejects: 145
Reflection shell
Resolution (Å)Redundancy (%)Rmerge(I) obsNum. unique allCC1/2Diffraction-ID% possible all
3.45-3.563.10.5657270.77199.7
14.22-43.142.90.0340.997189.2

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Phasing

PhasingMethod: molecular replacement

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Processing

Software
NameVersionClassification
SBC-Collectdata collection
MOSFLMdata reduction
Aimless0.5.17data scaling
PHASERphasing
Cootmodel building
PHENIX(1.11.1_2575: ???)refinement
PDB_EXTRACT3.22data extraction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4JHW

4jhw


Resolution: 3.45→43.139 Å / SU ML: 0.44 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 24.73
RfactorNum. reflection% reflectionSelection details
Rfree0.2324 2650 4.96 %Random
Rwork0.1747 ---
obs0.1775 53376 98.86 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 358.08 Å2 / Biso mean: 138.6 Å2 / Biso min: 53.57 Å2
Refinement stepCycle: final / Resolution: 3.45→43.139 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms20195 0 129 0 20324
Biso mean--158.48 --
Num. residues----2635
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00720737
X-RAY DIFFRACTIONf_angle_d0.96128224
X-RAY DIFFRACTIONf_chiral_restr0.0563359
X-RAY DIFFRACTIONf_plane_restr0.0063549
X-RAY DIFFRACTIONf_dihedral_angle_d3.97912535
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A6309X-RAY DIFFRACTION8.431TORSIONAL
12D6309X-RAY DIFFRACTION8.431TORSIONAL
13F6309X-RAY DIFFRACTION8.431TORSIONAL
21C3035X-RAY DIFFRACTION8.431TORSIONAL
22G3035X-RAY DIFFRACTION8.431TORSIONAL
23L3035X-RAY DIFFRACTION8.431TORSIONAL
31B2957X-RAY DIFFRACTION8.431TORSIONAL
32E2957X-RAY DIFFRACTION8.431TORSIONAL
33H2957X-RAY DIFFRACTION8.431TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 19

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
3.4501-3.51280.36191520.31172662281499
3.5128-3.58030.31661290.27726642793100
3.5803-3.65330.28651500.259526412791100
3.6533-3.73270.28921370.24622696283399
3.7327-3.81950.35891380.24872680281899
3.8195-3.9150.27061460.24132632277899
3.915-4.02070.25091340.21526942828100
4.0207-4.1390.2461120.19592697280999
4.139-4.27240.2391630.17062657282099
4.2724-4.4250.22311520.15382658281099
4.425-4.6020.17161200.15072654277499
4.602-4.81120.22381450.13712693283899
4.8112-5.06450.19781280.13762692282099
5.0645-5.38120.19861520.15362638279098
5.3812-5.79580.21411560.15432679283599
5.7958-6.37740.26021160.17312665278198
6.3774-7.29630.21711340.17872686282098
7.2963-9.1780.20091500.14982666281698
9.178-43.14270.22411360.15522672280896
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
17.48893.3247-0.13617.7993-1.89175.2192-0.21351.1665-0.7512-0.08860.25270.54070.6552-0.414-0.02630.6698-0.11450.10221.231-0.4011.3452-63.80312.967-52.471
27.20151.4621-1.54344.8949-0.7274.5394-0.1421-0.71610.25270.67160.10411.25770.44920.13760.01450.7685-0.030.08331.0688-0.25811.3292-65.99711.642-45.47
36.39164.92884.71834.81763.71053.47020.6035-2.4154-0.95921.1681-0.4522-1.0795-1.4642-3.19860.10592.43210.02890.85872.6015-0.02262.1509-86.17512.215-22.478
43.63681.38631.82291.7849-0.95683.86830.5994-1.73020.52530.47810.365-0.6648-0.9367-0.7952-0.73642.0715-0.51040.97112.1239-0.27182.1065-80.58713.298-27.423
56.10050.9671-2.13980.1795-0.38780.82620.01171.25691.08070.30160.31460.1426-0.8753-1.63730.05251.75910.23140.34052.4021-0.02482.3202-88.77918.783-31.546
62.9289-0.69942.57093.675-4.83877.70490.1012-0.634-0.05651.4263-0.2858-0.6870.16220.66510.13871.2235-0.1248-0.02311.524-0.2641.2035-48.63915.653-32.962
73.212.85911.08284.36823.0893.24562.339-2.26690.5992.1884-0.6282-0.8634-0.58690.8269-1.99561.8831-0.21470.23571.7749-0.13391.3864-54.64716.747-28.43
88.84031.52251.18615.9235-3.89513.43720.4539-1.5434-1.33631.5394-0.36060.12470.2123-0.024-0.39591.2625-0.15010.06491.1001-0.00181.1226-55.41111.339-29.163
93.06042.14881.65222.60342.60792.5940.4464-3.4361-0.37021.5336-1.23872.38910.7602-1.17270.66152.4403-0.29511.32383.12350.23163.0721-82.7135.878-16.592
105.41584.8958-4.35774.8367-4.89726.36870.4874-2.0413-1.11392.94541.33050.0378-1.9232-1.6054-1.60672.7079-0.40730.74982.60080.19812.5368-74.7465.702-17.714
110.17650.76-0.27385.0614-1.39540.80731.4124-4.1179-1.8650.0718-1.222-0.83820.22750.1481-0.33041.8264-0.74010.63023.4780.20612.2195-88.886-0.673-15.378
120.37540.23390.20471.8871.42021.0306-0.8948-2.1103-0.41220.3204-1.3599-0.0190.58171.0341.26862.1909-0.73791.04343.25780.93041.0209-81.3326.299-6.215
134.1786-0.1236-0.95390.29470.45871.37060.0080.5963-0.06240.01880.0003-0.09990.0860.18310.00110.68130.05-0.17420.8063-0.00230.9632-8.88523.939-61.255
146.9491-1.1294-3.03520.99050.87774.668-0.02340.2672-0.2256-0.1142-0.14320.40620.2435-0.38460.14070.6499-0.0341-0.16170.9115-0.04640.878-26.78620.022-64.341
157.05183.4319-3.21586.4011.50263.5372-0.0738-0.56410.34650.4834-0.32910.2192-0.565-0.10910.25870.66590.0533-0.22821.1169-0.10690.8405-35.57530.308-50.884
165.04011.1363-1.98041.8238-0.30583.8690.1456-0.383-0.20150.0533-0.0783-0.03030.22470.3462-0.0650.59680.0336-0.1010.6862-0.03790.7292-4.98522.053-62.95
172.01840.6711-0.13253.29720.74041.8713-0.1820.2246-0.024-0.09050.4363-0.5542-0.14990.4784-0.26240.5782-0.0383-0.08581.3836-0.10931.231217.51830.692-65.484
183.62460.6823-4.75783.7035-1.73545.4992-0.54470.4954-0.8145-0.831-0.0385-0.8761-0.59560.23780.67591.0416-0.380.16762.0706-0.12791.526729.9731.045-88.5
193.9343-0.97950.01892.1224-1.41146.84230.4151-0.6915-0.63270.38060.023-0.0680.34260.6473-0.43040.939-0.0766-0.11841.19620.37020.9059-15.61728.742-6.725
206.1375-3.10824.57396.4612-2.29913.49410.64960.43620.13530.425-0.73660.0946-0.22620.44340.14491.3956-0.22520.14161.80760.06910.8946-34.77733.23717.644
216.4028-3.4573-2.45836.36363.2092.1340.125-0.340.12980.11240.19040.5611-1.2391-0.5502-0.3221.40820.22770.15011.40910.5240.9474-27.62947.193-12.185
226.5415-2.77872.93289.3631-1.45452.16750.5310.24521.28551.1515-0.8272-0.5851-1.12240.15190.26681.7763-0.32060.45991.42910.07311.1716-36.54749.10723.22
231.30760.1361-1.11491.8265-0.67213.2746-0.13980.09410.4011-0.1830.1498-0.0232-0.23820.3685-0.02070.9012-0.0773-0.37230.8910.05331.1959-3.53446.441-57.734
244.51370.5463-3.01142.039-0.41083.9956-0.0728-0.682-0.19180.56040.216-0.4636-0.05860.9458-0.16370.76430.0958-0.29731.0706-0.01580.9472-4.0235.735-41.905
256.9766-2.07920.77814.0356-0.55249.04390.36410.3269-0.06720.173-0.14510.5716-0.6011-1.8507-0.20340.65250.0826-0.11210.9347-0.05550.8733-22.15534.352-37.508
264.04420.7148-3.20926.5489-3.75335.07-0.1987-0.24860.54270.34760.0592-0.0756-0.6925-0.09930.13620.71470.012-0.31550.8614-0.10641.0515-2.45846.773-50.123
274.0406-1.0796-0.4661.41751.28497.9199-0.38830.73770.877-0.50230.2875-0.2716-1.08151.05760.08161.1058-0.4169-0.11651.07670.32151.32755.87152.187-78.375
284.34940.9918-0.3391.23510.75164.8084-0.36630.80610.993-0.7568-0.0299-0.4444-0.83670.62070.45241.2323-0.3219-0.14861.15770.53061.40.02155.011-88.475
293.4477-1.0472-0.30451.65591.81961.41010.00091.22120.5042-0.42890.0269-0.8151-0.76640.94770.19811.5555-0.4340.11131.97890.33231.41615.59750.536-94.729
309.1745-1.63340.62564.4641-4.26038.78160.0151-0.5088-0.20.79880.0475-0.5492-1.5978-1.184-0.03551.40480.3588-0.29551.2343-0.34130.8856-51.79179.303-35.453
313.85720.9285-3.20753.36693.10867.62360.7092-0.4592-0.43790.4534-0.3659-0.52890.03780.0052-0.45791.17780.3344-0.16820.8971-0.18181.0413-46.95171.805-44.258
329.8583-1.4246-0.94165.28680.82959.61050.6958-0.02941.80690.23120.5308-1.3472-1.6342-0.1623-1.43351.29710.26060.00660.7124-0.19331.4154-45.90279.806-43.696
338.9558-2.4791-6.08243.44574.42966.96710.17911.2109-0.65320.03780.062-0.0901-0.8699-1.2574-0.22091.15060.2408-0.13560.8402-0.21220.8852-55.48176.466-44.332
343.2228-4.442-2.90076.2774.18483.17920.33850.04480.31820.9016-0.1339-0.87820.60430.1755-0.29041.20060.3518-0.23910.8829-0.06961.0604-46.33267.885-41.706
355.32230.7929-3.44124.4429-4.97177.56710.26220.1228-0.6065-0.3912-1.0619-0.1682-0.2058-1.12560.58431.28590.3971-0.07951.7605-0.33160.9857-75.2679.899-27.755
366.32270.6367-4.40328.502-0.23753.15060.49860.3127-1.6430.0835-1.02780.7652-0.9908-1.88290.32510.93970.148-0.08971.6842-0.17221.4924-78.35472.518-24.53
379.37733.2963-7.98489.0474-2.74819.66790.16150.3452-1.39130.6788-0.1829-1.5805-1.1095-0.5753-0.28021.3759-0.008-0.07281.8298-0.47471.02-73.9781.793-21.083
386.67762.4582-0.75335.225-0.39244.61520.35980.7452-1.0725-0.23-0.50820.86890.2006-0.80350.20571.04210.3065-0.24251.1682-0.52361.4423-59.35254.869-44.713
391.1544-0.7632-0.84128.2740.58562.19110.10790.5732-0.8432-1.1068-0.83291.8416-0.5736-1.09010.65541.22350.293-0.33382.2816-0.75871.7488-89.74271.55-32.809
403.9003-1.7383-3.42053.54534.06335.5331-0.15430.42520.5384-0.44150.0505-0.153-0.5077-0.0920.17150.7224-0.0149-0.35121.04220.26231.0539-19.95140.335-73.697
414.0992-0.5573-2.0320.72271.26585.9618-0.0110.4481.3359-0.1735-0.0173-0.2064-1.4154-0.6470.02311.150.1418-0.40660.80250.12231.4943-24.51555.511-63.502
425.9546-1.5687-0.27964.6823-1.49551.4382-0.595-0.3467-0.11050.9210.2427-0.61460.4442-0.45190.21830.8250.1521-0.31230.864-0.16871.0779-31.32848.648-47.196
433.13231.23980.25797.29056.85788.5847-0.27520.15490.3373-0.4688-0.11020.6344-0.5679-0.22340.48110.85780.1271-0.28780.86520.27361.1717-25.14445.589-72.941
443.0427-2.4366-3.55718.13013.09534.4514-0.79870.2150.1131-0.26310.70350.1407-0.14860.19280.28710.7938-0.1832-0.04671.21330.10730.7134-6.32229.213-89.381
454.3016-0.4601-1.40664.9674-1.27453.9228-0.28721.0028-0.2455-0.4730.4060.0272-0.00640.4002-0.08350.7716-0.1373-0.05851.2807-0.16210.8103-2.43318.008-88.984
469.51262.096-5.79182.3846-3.65785.8625-0.04681.8543-0.312-0.36450.0667-0.8967-0.0876-0.1164-0.10041.0371-0.23010.15121.7315-0.27041.18879.58724.929-100.076
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1( CHAIN H AND RESID 2:82 )H2 - 82
2X-RAY DIFFRACTION2( CHAIN H AND RESID 83:124 )H83 - 124
3X-RAY DIFFRACTION3( CHAIN H AND RESID 125:145 )H125 - 145
4X-RAY DIFFRACTION4( CHAIN H AND RESID 146:188 )H146 - 188
5X-RAY DIFFRACTION5( CHAIN H AND RESID 189:211 )H189 - 211
6X-RAY DIFFRACTION6( CHAIN L AND RESID 1:75 )L1 - 75
7X-RAY DIFFRACTION7( CHAIN L AND RESID 76:90 )L76 - 90
8X-RAY DIFFRACTION8( CHAIN L AND RESID 91:113 )L91 - 113
9X-RAY DIFFRACTION9( CHAIN L AND RESID 114:150 )L114 - 150
10X-RAY DIFFRACTION10( CHAIN L AND RESID 151:174 )L151 - 174
11X-RAY DIFFRACTION11( CHAIN L AND RESID 175:197 )L175 - 197
12X-RAY DIFFRACTION12( CHAIN L AND RESID 198:211 )L198 - 211
13X-RAY DIFFRACTION13( CHAIN F AND RESID 27:74 )F27 - 74
14X-RAY DIFFRACTION14( CHAIN F AND RESID 75:201 )F75 - 201
15X-RAY DIFFRACTION15( CHAIN F AND RESID 202:239 )F202 - 239
16X-RAY DIFFRACTION16( CHAIN F AND RESID 240:332 )F240 - 332
17X-RAY DIFFRACTION17( CHAIN F AND RESID 333:469 )F333 - 469
18X-RAY DIFFRACTION18( CHAIN F AND RESID 470:513 )F470 - 513
19X-RAY DIFFRACTION19( CHAIN B AND RESID 2:111 )B2 - 111
20X-RAY DIFFRACTION20( CHAIN B AND RESID 112:213 )B112 - 213
21X-RAY DIFFRACTION21( CHAIN C AND RESID 1:113 )C1 - 113
22X-RAY DIFFRACTION22( CHAIN C AND RESID 114:211 )C114 - 211
23X-RAY DIFFRACTION23( CHAIN A AND RESID 27:73 )A27 - 73
24X-RAY DIFFRACTION24( CHAIN A AND RESID 74:201 )A74 - 201
25X-RAY DIFFRACTION25( CHAIN A AND RESID 202:239 )A202 - 239
26X-RAY DIFFRACTION26( CHAIN A AND RESID 240:309 )A240 - 309
27X-RAY DIFFRACTION27( CHAIN A AND RESID 310:371 )A310 - 371
28X-RAY DIFFRACTION28( CHAIN A AND RESID 372:458 )A372 - 458
29X-RAY DIFFRACTION29( CHAIN A AND RESID 459:512 )A459 - 512
30X-RAY DIFFRACTION30( CHAIN E AND RESID 1:28 )E1 - 28
31X-RAY DIFFRACTION31( CHAIN E AND RESID 29:59 )E29 - 59
32X-RAY DIFFRACTION32( CHAIN E AND RESID 60:82 )E60 - 82
33X-RAY DIFFRACTION33( CHAIN E AND RESID 83:96 )E83 - 96
34X-RAY DIFFRACTION34( CHAIN E AND RESID 97:111 )E97 - 111
35X-RAY DIFFRACTION35( CHAIN E AND RESID 112:157 )E112 - 157
36X-RAY DIFFRACTION36( CHAIN E AND RESID 158:194 )E158 - 194
37X-RAY DIFFRACTION37( CHAIN E AND RESID 195:215 )E195 - 215
38X-RAY DIFFRACTION38( CHAIN G AND RESID 1:113 )G1 - 113
39X-RAY DIFFRACTION39( CHAIN G AND RESID 114:213 )G114 - 213
40X-RAY DIFFRACTION40( CHAIN D AND RESID 27:73 )D27 - 73
41X-RAY DIFFRACTION41( CHAIN D AND RESID 74:201 )D74 - 201
42X-RAY DIFFRACTION42( CHAIN D AND RESID 202:239 )D202 - 239
43X-RAY DIFFRACTION43( CHAIN D AND RESID 240:309 )D240 - 309
44X-RAY DIFFRACTION44( CHAIN D AND RESID 310:371 )D310 - 371
45X-RAY DIFFRACTION45( CHAIN D AND RESID 372:458 )D372 - 458
46X-RAY DIFFRACTION46( CHAIN D AND RESID 459:513 )D459 - 513

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