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- PDB-5udd: Crystal Structure of RSV F B9320 Bound to MEDI8897 -

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Basic information

Entry
Database: PDB / ID: 5udd
TitleCrystal Structure of RSV F B9320 Bound to MEDI8897
Components
  • Fusion glycoprotein F0
  • MEDI8897 Fab Heavy Chain
  • MEDI8897 Fab Light Chain
KeywordsViral Protein/Immune System / immune system / antibody / fusion glycoprotein / virus / Viral Protein-Immune System complex
Function / homology
Function and homology information


symbiont-mediated induction of syncytium formation / host cell Golgi membrane / entry receptor-mediated virion attachment to host cell / fusion of virus membrane with host plasma membrane / viral envelope / symbiont entry into host cell / host cell plasma membrane / virion membrane / membrane
Similarity search - Function
Precursor fusion glycoprotein F0, Paramyxoviridae / Fusion glycoprotein F0
Similarity search - Domain/homology
Fusion glycoprotein F0
Similarity search - Component
Biological speciesHuman Respiratory syncytial virus 9320
Homo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 4.3 Å
AuthorsMcLellan, J.S.
CitationJournal: Sci Transl Med / Year: 2017
Title: A highly potent extended half-life antibody as a potential RSV vaccine surrogate for all infants.
Authors: Zhu, Q. / McLellan, J.S. / Kallewaard, N.L. / Ulbrandt, N.D. / Palaszynski, S. / Zhang, J. / Moldt, B. / Khan, A. / Svabek, C. / McAuliffe, J.M. / Wrapp, D. / Patel, N.K. / Cook, K.E. / ...Authors: Zhu, Q. / McLellan, J.S. / Kallewaard, N.L. / Ulbrandt, N.D. / Palaszynski, S. / Zhang, J. / Moldt, B. / Khan, A. / Svabek, C. / McAuliffe, J.M. / Wrapp, D. / Patel, N.K. / Cook, K.E. / Richter, B.W.M. / Ryan, P.C. / Yuan, A.Q. / Suzich, J.A.
History
DepositionDec 26, 2016Deposition site: RCSB / Processing site: RCSB
Revision 1.0May 17, 2017Provider: repository / Type: Initial release
Revision 1.1Oct 4, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id
Revision 1.2Nov 20, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Fusion glycoprotein F0
B: Fusion glycoprotein F0
C: Fusion glycoprotein F0
D: Fusion glycoprotein F0
E: Fusion glycoprotein F0
F: Fusion glycoprotein F0
G: Fusion glycoprotein F0
H: Fusion glycoprotein F0
I: Fusion glycoprotein F0
J: MEDI8897 Fab Heavy Chain
K: MEDI8897 Fab Heavy Chain
L: MEDI8897 Fab Heavy Chain
M: MEDI8897 Fab Heavy Chain
N: MEDI8897 Fab Heavy Chain
O: MEDI8897 Fab Heavy Chain
P: MEDI8897 Fab Light Chain
Q: MEDI8897 Fab Light Chain
R: MEDI8897 Fab Light Chain
S: MEDI8897 Fab Light Chain
T: MEDI8897 Fab Light Chain
U: MEDI8897 Fab Light Chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)856,99536
Polymers855,55421
Non-polymers1,44115
Water00
1
A: Fusion glycoprotein F0
B: Fusion glycoprotein F0
C: Fusion glycoprotein F0
J: MEDI8897 Fab Heavy Chain
K: MEDI8897 Fab Heavy Chain
P: MEDI8897 Fab Light Chain
Q: MEDI8897 Fab Light Chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)285,95315
Polymers285,1857
Non-polymers7698
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
D: Fusion glycoprotein F0
E: Fusion glycoprotein F0
F: Fusion glycoprotein F0
L: MEDI8897 Fab Heavy Chain
M: MEDI8897 Fab Heavy Chain
R: MEDI8897 Fab Light Chain
S: MEDI8897 Fab Light Chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)285,47310
Polymers285,1857
Non-polymers2883
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
G: Fusion glycoprotein F0
H: Fusion glycoprotein F0
I: Fusion glycoprotein F0
N: MEDI8897 Fab Heavy Chain
O: MEDI8897 Fab Heavy Chain
T: MEDI8897 Fab Light Chain
U: MEDI8897 Fab Light Chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)285,56911
Polymers285,1857
Non-polymers3844
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)229.020, 229.020, 343.250
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number154
Space group name H-MP3221
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain P and (resid 1 through 107 or resid 111 through 211))
21(chain Q and (resid 1 through 107 or resid 111 through 211))
31(chain R and (resid 1 through 107 or resid 111 through 211))
41(chain S and (resid 1 through 107 or resid 111 through 211))
51(chain T and (resid 1 through 107 or resid 111 through 211))
61chain U
12(chain J and (resid 1 through 113 or resid 116 through 126 or resid 135 through 213))
22(chain K and (resid 1 through 113 or resid 116 through 126 or resid 135 through 213))
32(chain L and (resid 1 through 113 or resid 116 through 126 or resid 135 through 213))
42(chain M and (resid 1 through 113 or resid 116 through 126 or resid 135 through 213))
52(chain N and (resid 1 through 113 or resid 116 through 126 or resid 135 through 213))
62chain O
13(chain A and (resid 27 through 97 or resid 138 through 512))
23(chain B and (resid 27 through 97 or resid 138 through 512))
33(chain C and (resid 27 through 97 or resid 138 through 512))
43(chain D and (resid 27 through 97 or resid 138 through 512))
53(chain E and (resid 27 through 97 or resid 138 through 512))
63(chain F and (resid 27 through 97 or resid 138 through 512))
73(chain G and (resid 27 through 97 or resid 138 through 512))
83(chain H and (resid 27 through 97 or resid 138 through 512))
93(chain I and (resid 27 through 97 or resid 138 through 512))

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111ASPASPLYSLYS(chain P and (resid 1 through 107 or resid 111 through 211))PP1 - 1071 - 107
121ALAALAARGARG(chain P and (resid 1 through 107 or resid 111 through 211))PP111 - 211111 - 211
211ASPASPLYSLYS(chain Q and (resid 1 through 107 or resid 111 through 211))QQ1 - 1071 - 107
221ALAALAARGARG(chain Q and (resid 1 through 107 or resid 111 through 211))QQ111 - 211111 - 211
311ASPASPLYSLYS(chain R and (resid 1 through 107 or resid 111 through 211))RR1 - 1071 - 107
321ALAALAARGARG(chain R and (resid 1 through 107 or resid 111 through 211))RR111 - 211111 - 211
411ASPASPLYSLYS(chain S and (resid 1 through 107 or resid 111 through 211))SS1 - 1071 - 107
421ALAALAARGARG(chain S and (resid 1 through 107 or resid 111 through 211))SS111 - 211111 - 211
511ASPASPLYSLYS(chain T and (resid 1 through 107 or resid 111 through 211))TT1 - 1071 - 107
521ALAALAARGARG(chain T and (resid 1 through 107 or resid 111 through 211))TT111 - 211111 - 211
611ASPASPARGARGchain UUU1 - 2111 - 211
112GLNGLNSERSER(chain J and (resid 1 through 113 or resid 116 through 126 or resid 135 through 213))JJ1 - 1131 - 126
122THRTHRPROPRO(chain J and (resid 1 through 113 or resid 116 through 126 or resid 135 through 213))JJ116 - 126129 - 139
132THRTHRPROPRO(chain J and (resid 1 through 113 or resid 116 through 126 or resid 135 through 213))JJ135 - 213148 - 226
212GLNGLNSERSER(chain K and (resid 1 through 113 or resid 116 through 126 or resid 135 through 213))KK1 - 1131 - 126
222THRTHRPROPRO(chain K and (resid 1 through 113 or resid 116 through 126 or resid 135 through 213))KK116 - 126129 - 139
232THRTHRPROPRO(chain K and (resid 1 through 113 or resid 116 through 126 or resid 135 through 213))KK135 - 213148 - 226
312GLNGLNSERSER(chain L and (resid 1 through 113 or resid 116 through 126 or resid 135 through 213))LL1 - 1131 - 126
322THRTHRPROPRO(chain L and (resid 1 through 113 or resid 116 through 126 or resid 135 through 213))LL116 - 126129 - 139
332THRTHRPROPRO(chain L and (resid 1 through 113 or resid 116 through 126 or resid 135 through 213))LL135 - 213148 - 226
412GLNGLNSERSER(chain M and (resid 1 through 113 or resid 116 through 126 or resid 135 through 213))MM1 - 1131 - 126
422THRTHRPROPRO(chain M and (resid 1 through 113 or resid 116 through 126 or resid 135 through 213))MM116 - 126129 - 139
432THRTHRPROPRO(chain M and (resid 1 through 113 or resid 116 through 126 or resid 135 through 213))MM135 - 213148 - 226
512GLNGLNSERSER(chain N and (resid 1 through 113 or resid 116 through 126 or resid 135 through 213))NN1 - 1131 - 126
522THRTHRPROPRO(chain N and (resid 1 through 113 or resid 116 through 126 or resid 135 through 213))NN116 - 126129 - 139
532THRTHRPROPRO(chain N and (resid 1 through 113 or resid 116 through 126 or resid 135 through 213))NN135 - 213148 - 226
612GLNGLNPROPROchain OOO1 - 2131 - 226
113ASNASNTHRTHR(chain A and (resid 27 through 97 or resid 138 through 512))AA27 - 9727 - 97
123LEULEULEULEU(chain A and (resid 27 through 97 or resid 138 through 512))AA138 - 512138 - 512
213ASNASNTHRTHR(chain B and (resid 27 through 97 or resid 138 through 512))BB27 - 9727 - 97
223LEULEULEULEU(chain B and (resid 27 through 97 or resid 138 through 512))BB138 - 512138 - 512
313ASNASNTHRTHR(chain C and (resid 27 through 97 or resid 138 through 512))CC27 - 9727 - 97
323LEULEULEULEU(chain C and (resid 27 through 97 or resid 138 through 512))CC138 - 512138 - 512
413ASNASNTHRTHR(chain D and (resid 27 through 97 or resid 138 through 512))DD27 - 9727 - 97
423LEULEULEULEU(chain D and (resid 27 through 97 or resid 138 through 512))DD138 - 512138 - 512
513ASNASNTHRTHR(chain E and (resid 27 through 97 or resid 138 through 512))EE27 - 9727 - 97
523LEULEULEULEU(chain E and (resid 27 through 97 or resid 138 through 512))EE138 - 512138 - 512
613ASNASNTHRTHR(chain F and (resid 27 through 97 or resid 138 through 512))FF27 - 9727 - 97
623LEULEULEULEU(chain F and (resid 27 through 97 or resid 138 through 512))FF138 - 512138 - 512
713ASNASNTHRTHR(chain G and (resid 27 through 97 or resid 138 through 512))GG27 - 9727 - 97
723LEULEULEULEU(chain G and (resid 27 through 97 or resid 138 through 512))GG138 - 512138 - 512
813ASNASNTHRTHR(chain H and (resid 27 through 97 or resid 138 through 512))HH27 - 9727 - 97
823LEULEULEULEU(chain H and (resid 27 through 97 or resid 138 through 512))HH138 - 512138 - 512
913ASNASNTHRTHR(chain I and (resid 27 through 97 or resid 138 through 512))II27 - 9727 - 97
923LEULEULEULEU(chain I and (resid 27 through 97 or resid 138 through 512))II138 - 512138 - 512

NCS ensembles :
ID
1
2
3

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Components

#1: Protein
Fusion glycoprotein F0


Mass: 63406.281 Da / Num. of mol.: 9 / Mutation: S155C, S290C, S190F, V207L
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Human Respiratory syncytial virus 9320 / Plasmid: paH / Cell line (production host): 293F / Production host: Homo sapiens (human) / References: UniProt: Q6V2E7
#2: Antibody
MEDI8897 Fab Heavy Chain


Mass: 24157.061 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): 293F / Production host: Homo sapiens (human)
#3: Antibody
MEDI8897 Fab Light Chain


Mass: 23325.865 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): 293F / Production host: Homo sapiens (human)
#4: Chemical
ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 15 / Source method: obtained synthetically / Formula: SO4
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.04 Å3/Da / Density % sol: 59.5 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8
Details: 0.1 M Tris pH 8.0, 1.4 M ammonium sulfate, 0.2 M sodium thiocyanate

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.97924 Å
DetectorType: ADSC QUANTUM 315 / Detector: CCD / Date: Nov 10, 2014
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97924 Å / Relative weight: 1
ReflectionResolution: 4.3→46.311 Å / Num. obs: 71246 / % possible obs: 99.9 % / Redundancy: 7.2 % / Biso Wilson estimate: 91.02 Å2 / CC1/2: 0.971 / Rmerge(I) obs: 0.567 / Net I/σ(I): 3.7
Reflection shell
Resolution (Å)Redundancy (%)Rmerge(I) obsNum. unique allCC1/2Diffraction-ID% possible all
4.3-4.47.51.3766620.6271100
20.62-46.315.30.0790.997190

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Phasing

PhasingMethod: molecular replacement

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Processing

Software
NameVersionClassification
SBC-Collectdata collection
MOSFLMdata reduction
Aimless0.3.11data scaling
PHASERphasing
Cootmodel building
PHENIX(1.11.1_2575: ???)refinement
PDB_EXTRACT3.22data extraction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 5UDE

5ude


Resolution: 4.3→46.311 Å / SU ML: 0.59 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 30.49
RfactorNum. reflection% reflectionSelection details
Rfree0.2945 3459 4.86 %Random
Rwork0.2365 ---
obs0.2393 71119 99.87 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 248.06 Å2 / Biso mean: 101.7324 Å2 / Biso min: 56.74 Å2
Refinement stepCycle: final / Resolution: 4.3→46.311 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms51126 0 75 0 51201
Biso mean--128.88 --
Num. residues----6648
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00952169
X-RAY DIFFRACTIONf_angle_d0.79870938
X-RAY DIFFRACTIONf_chiral_restr0.0498399
X-RAY DIFFRACTIONf_plane_restr0.0058949
X-RAY DIFFRACTIONf_dihedral_angle_d3.44431484
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11P5970X-RAY DIFFRACTION10.213TORSIONAL
12Q5970X-RAY DIFFRACTION10.213TORSIONAL
13R5970X-RAY DIFFRACTION10.213TORSIONAL
14S5970X-RAY DIFFRACTION10.213TORSIONAL
15T5970X-RAY DIFFRACTION10.213TORSIONAL
16U5970X-RAY DIFFRACTION10.213TORSIONAL
21J5986X-RAY DIFFRACTION10.213TORSIONAL
22K5986X-RAY DIFFRACTION10.213TORSIONAL
23L5986X-RAY DIFFRACTION10.213TORSIONAL
24M5986X-RAY DIFFRACTION10.213TORSIONAL
25N5986X-RAY DIFFRACTION10.213TORSIONAL
26O5986X-RAY DIFFRACTION10.213TORSIONAL
31A19372X-RAY DIFFRACTION10.213TORSIONAL
32B19372X-RAY DIFFRACTION10.213TORSIONAL
33C19372X-RAY DIFFRACTION10.213TORSIONAL
34D19372X-RAY DIFFRACTION10.213TORSIONAL
35E19372X-RAY DIFFRACTION10.213TORSIONAL
36F19372X-RAY DIFFRACTION10.213TORSIONAL
37G19372X-RAY DIFFRACTION10.213TORSIONAL
38H19372X-RAY DIFFRACTION10.213TORSIONAL
39I19372X-RAY DIFFRACTION10.213TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 25

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
4.3001-4.35890.34871500.283226372787100
4.3589-4.42120.3271140.280726612775100
4.4212-4.48710.32041550.271627062861100
4.4871-4.55720.28161280.253926662794100
4.5572-4.63180.31211630.252226412804100
4.6318-4.71160.29151560.250626532809100
4.7116-4.79720.29031170.244327322849100
4.7972-4.88940.31111500.248526632813100
4.8894-4.98910.31321440.251726792823100
4.9891-5.09740.28221280.256927262854100
5.0974-5.21580.28861200.253826952815100
5.2158-5.34610.3051360.255826752811100
5.3461-5.49040.32211490.265926782827100
5.4904-5.65170.30321090.268127352844100
5.6517-5.83380.39531660.269327042870100
5.8338-6.04190.32191210.280126882809100
6.0419-6.28320.32121350.265227082843100
6.2832-6.56840.34441260.247627392865100
6.5684-6.91360.32311300.241427132843100
6.9136-7.34520.31011630.247127012864100
7.3452-7.90980.30811390.218227232862100
7.9098-8.7010.24471630.185527072870100
8.701-9.94920.19961180.160528072925100
9.9492-12.4940.18921350.15512778291399
12.494-46.31320.3241440.24212845298998
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.7241-0.05860.57890.74280.06420.81980.12690.2474-0.1571-0.0601-0.0787-0.04880.18710.062-0.05530.89550.1172-0.00820.7091-0.03350.5272-17.771-23.57533.423
20.76610.1415-0.2911.26270.18320.99140.0540.1726-0.0211-0.1088-0.041-0.1332-0.07120.0601-0.00830.72160.0024-0.01350.73470.00070.5556-88.299-67.95133.916
30.87070.18820.00911.3791-0.23860.78210.04280.05360.0394-0.1109-0.11980.1846-0.1238-0.10630.06330.76530.0823-0.01820.7894-0.10610.6263-14.183-106.31432.812
45.2039-1.7013-0.43872.4619-1.36893.78940.0109-0.05410.59-0.14120.1225-0.4589-0.39240.3399-0.13560.82-0.40730.12650.86-0.13670.7298-73.326-21.34362.358
53.98461.0681-1.71624.3267-1.50214.06711.02550.13690.72640.4795-0.17751.3499-0.8701-0.8929-0.72281.20990.01990.36370.6416-0.05481.1695-106.861-20.53268.212
64.4988-1.65780.02231.6534-0.09162.4161-0.36850.25870.7801-0.04610.0828-0.0013-1.8317-1.22570.35031.82450.5058-0.24441.177-0.19270.8441-73.262-1.44815.484
74.6280.1667-0.28513.57870.77410.1535-0.0989-1.40110.57860.008-0.18860.8703-1.0114-1.85880.28851.7511.0925-0.15793.0531-0.16481.3456-106.9992.43312.887
83.42-1.53861.76963.3149-0.30482.5508-0.3874-0.9844-0.50560.55060.47460.434-0.0695-0.377-0.09940.90130.02230.17821.14520.25320.7108-62.365-117.95860.959
93.2483-0.67992.0843.64422.05665.7547-0.0097-0.9791-1.6894-0.13380.7520.58310.3183-0.6531-0.67890.8257-0.02190.15911.05420.471.5673-46.119-147.24565.462
106.05321.87860.38486.06382.17393.5815-0.3893-0.12320.00740.01520.4269-0.2864-0.71490.1508-0.00990.6832-0.05020.10160.4768-0.01150.452-80.09-127.07215.353
116.4464-0.63011.51883.95040.84722.9079-0.5583-0.6311-1.34840.47170.4309-0.33391.22280.08560.14971.20040.19050.25430.63660.04591.0393-67.453-158.62313.528
125.09711.7208-0.38775.18960.7954.26980.0896-0.6154-0.10180.1198-0.2602-0.50920.22140.32120.12450.77870.0472-0.17520.7011-0.01640.657316.099-59.64761.599
135.37731.6965-1.71984.23431.23386.1726-0.1399-0.73080.42060.70550.0189-1.235-0.45190.56670.10460.93760.0424-0.37860.69550.11541.031633.333-31.166.684
142.81180.0372-0.42441.5488-1.30533.2806-0.36950.4340.27941.2239-0.7682-0.4281-1.75313.88140.59290.7038-0.3019-0.19382.56190.31440.796434.48-71.94115.217
152.095-1.59460.77654.50890.29570.33980.40750.59530.52021.3387-1.1344-1.2056-0.69161.65180.67281.6177-0.9983-0.22072.69560.23971.591559.18-48.80311.954
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1( CHAIN A AND RESID 26:513 ) OR ( CHAIN C AND RESID 27:514 ) OR ( CHAIN B AND RESID 26:515 )A26 - 513
2X-RAY DIFFRACTION1( CHAIN A AND RESID 26:513 ) OR ( CHAIN C AND RESID 27:514 ) OR ( CHAIN B AND RESID 26:515 )C27 - 514
3X-RAY DIFFRACTION1( CHAIN A AND RESID 26:513 ) OR ( CHAIN C AND RESID 27:514 ) OR ( CHAIN B AND RESID 26:515 )B26 - 515
4X-RAY DIFFRACTION2( CHAIN E AND RESID 26:514 ) OR ( CHAIN D AND RESID 26:513 ) OR ( CHAIN F AND RESID 26:513 )E26 - 514
5X-RAY DIFFRACTION2( CHAIN E AND RESID 26:514 ) OR ( CHAIN D AND RESID 26:513 ) OR ( CHAIN F AND RESID 26:513 )D26 - 513
6X-RAY DIFFRACTION2( CHAIN E AND RESID 26:514 ) OR ( CHAIN D AND RESID 26:513 ) OR ( CHAIN F AND RESID 26:513 )F26 - 513
7X-RAY DIFFRACTION3( CHAIN I AND RESID 26:512 ) OR ( CHAIN H AND RESID 27:515 ) OR ( CHAIN G AND RESID 27:513 )I26 - 512
8X-RAY DIFFRACTION3( CHAIN I AND RESID 26:512 ) OR ( CHAIN H AND RESID 27:515 ) OR ( CHAIN G AND RESID 27:513 )H27 - 515
9X-RAY DIFFRACTION3( CHAIN I AND RESID 26:512 ) OR ( CHAIN H AND RESID 27:515 ) OR ( CHAIN G AND RESID 27:513 )G27 - 513
10X-RAY DIFFRACTION4( CHAIN P AND RESID 1:109 ) OR ( CHAIN J AND RESID 1:114 )P1 - 109
11X-RAY DIFFRACTION4( CHAIN P AND RESID 1:109 ) OR ( CHAIN J AND RESID 1:114 )J1 - 114
12X-RAY DIFFRACTION5( CHAIN P AND RESID 110:212 ) OR ( CHAIN J AND RESID 115:215 )P110 - 212
13X-RAY DIFFRACTION5( CHAIN P AND RESID 110:212 ) OR ( CHAIN J AND RESID 115:215 )J115 - 215
14X-RAY DIFFRACTION6( CHAIN Q AND RESID 1:109 ) OR ( CHAIN K AND RESID 1:114 )Q1 - 109
15X-RAY DIFFRACTION6( CHAIN Q AND RESID 1:109 ) OR ( CHAIN K AND RESID 1:114 )K1 - 114
16X-RAY DIFFRACTION7( CHAIN Q AND RESID 110:211 ) OR ( CHAIN K AND RESID 115:213 )Q110 - 211
17X-RAY DIFFRACTION7( CHAIN Q AND RESID 110:211 ) OR ( CHAIN K AND RESID 115:213 )K115 - 213
18X-RAY DIFFRACTION8( CHAIN R AND RESID 1:109 ) OR ( CHAIN L AND RESID 1:114 )R1 - 109
19X-RAY DIFFRACTION8( CHAIN R AND RESID 1:109 ) OR ( CHAIN L AND RESID 1:114 )L1 - 114
20X-RAY DIFFRACTION9( CHAIN R AND RESID 110:211 ) OR ( CHAIN L AND RESID 115:213 )R110 - 211
21X-RAY DIFFRACTION9( CHAIN R AND RESID 110:211 ) OR ( CHAIN L AND RESID 115:213 )L115 - 213
22X-RAY DIFFRACTION10( CHAIN S AND RESID 1:109 ) OR ( CHAIN M AND RESID 1:114 )S1 - 109
23X-RAY DIFFRACTION10( CHAIN S AND RESID 1:109 ) OR ( CHAIN M AND RESID 1:114 )M1 - 114
24X-RAY DIFFRACTION11( CHAIN S AND RESID 110:211 ) OR ( CHAIN M AND RESID 115:213 )S110 - 211
25X-RAY DIFFRACTION11( CHAIN S AND RESID 110:211 ) OR ( CHAIN M AND RESID 115:213 )M115 - 213
26X-RAY DIFFRACTION12( CHAIN T AND RESID 1:109 ) OR ( CHAIN N AND RESID 1:114 )T1 - 109
27X-RAY DIFFRACTION12( CHAIN T AND RESID 1:109 ) OR ( CHAIN N AND RESID 1:114 )N1 - 114
28X-RAY DIFFRACTION13( CHAIN T AND RESID 110:211 ) OR ( CHAIN N AND RESID 115:213 )T110 - 211
29X-RAY DIFFRACTION13( CHAIN T AND RESID 110:211 ) OR ( CHAIN N AND RESID 115:213 )N115 - 213
30X-RAY DIFFRACTION14( CHAIN U AND RESID 1:107 ) OR ( CHAIN O AND RESID 1:113 )U1 - 107
31X-RAY DIFFRACTION14( CHAIN U AND RESID 1:107 ) OR ( CHAIN O AND RESID 1:113 )O1 - 113
32X-RAY DIFFRACTION15( CHAIN U AND RESID 111:211 ) OR ( CHAIN O AND RESID 116:213 )U111 - 211
33X-RAY DIFFRACTION15( CHAIN U AND RESID 111:211 ) OR ( CHAIN O AND RESID 116:213 )O116 - 213

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