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- PDB-5tzt: Crystal structure of human CD47 ECD bound to Fab of C47B161 -

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Basic information

Entry
Database: PDB / ID: 5tzt
TitleCrystal structure of human CD47 ECD bound to Fab of C47B161
Components
  • Heavy Chain of Fab C47B161
  • Leukocyte surface antigen CD47
  • Light Chain of Fab C47B161
KeywordsIMMUNE SYSTEM / antigen-Fab complex / immunoglobulin
Function / homology
Function and homology information


cellular response to interleukin-12 / regulation of Fc receptor mediated stimulatory signaling pathway / protein binding involved in heterotypic cell-cell adhesion / positive regulation of monocyte extravasation / regulation of type II interferon production / ATP export / positive regulation of cell-cell adhesion / regulation of interleukin-10 production / cell-cell adhesion mediator activity / regulation of tumor necrosis factor production ...cellular response to interleukin-12 / regulation of Fc receptor mediated stimulatory signaling pathway / protein binding involved in heterotypic cell-cell adhesion / positive regulation of monocyte extravasation / regulation of type II interferon production / ATP export / positive regulation of cell-cell adhesion / regulation of interleukin-10 production / cell-cell adhesion mediator activity / regulation of tumor necrosis factor production / regulation of interleukin-12 production / regulation of nitric oxide biosynthetic process / negative regulation of phagocytosis / regulation of interleukin-6 production / Signal regulatory protein family interactions / thrombospondin receptor activity / tertiary granule membrane / cellular response to interleukin-1 / Integrin cell surface interactions / specific granule membrane / positive regulation of phagocytosis / positive regulation of stress fiber assembly / integrin-mediated signaling pathway / Cell surface interactions at the vascular wall / cellular response to type II interferon / positive regulation of inflammatory response / cell migration / positive regulation of T cell activation / angiogenesis / inflammatory response / apoptotic process / positive regulation of cell population proliferation / Neutrophil degranulation / cell surface / extracellular exosome / plasma membrane
Similarity search - Function
Leukocyte surface antigen CD47 / CD47-like, transmembrane / CD47 immunoglobulin-like / Leukocyte surface antigen CD47, IgV / CD47 transmembrane region / CD47 immunoglobulin-like domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulins ...Leukocyte surface antigen CD47 / CD47-like, transmembrane / CD47 immunoglobulin-like / Leukocyte surface antigen CD47, IgV / CD47 transmembrane region / CD47 immunoglobulin-like domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulins / Immunoglobulin-like fold / Immunoglobulin-like / Sandwich / Mainly Beta
Similarity search - Domain/homology
Leukocyte surface antigen CD47
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.89 Å
AuthorsCardoso, R.M.F.
CitationJournal: Blood Cancer J / Year: 2017
Title: Anti-leukemic activity and tolerability of anti-human CD47 monoclonal antibodies.
Authors: Pietsch, E.C. / Dong, J. / Cardoso, R. / Zhang, X. / Chin, D. / Hawkins, R. / Dinh, T. / Zhou, M. / Strake, B. / Feng, P.H. / Rocca, M. / Santos, C.D. / Shan, X. / Danet-Desnoyers, G. / Shi, ...Authors: Pietsch, E.C. / Dong, J. / Cardoso, R. / Zhang, X. / Chin, D. / Hawkins, R. / Dinh, T. / Zhou, M. / Strake, B. / Feng, P.H. / Rocca, M. / Santos, C.D. / Shan, X. / Danet-Desnoyers, G. / Shi, F. / Kaiser, E. / Millar, H.J. / Fenton, S. / Swanson, R. / Nemeth, J.A. / Attar, R.M.
History
DepositionNov 22, 2016Deposition site: RCSB / Processing site: RCSB
Revision 1.0Mar 15, 2017Provider: repository / Type: Initial release
Revision 1.1Nov 22, 2017Group: Refinement description / Category: software / Item: _software.name
Revision 2.0Jul 29, 2020Group: Data collection / Derived calculations ...Data collection / Derived calculations / Polymer sequence / Structure summary
Category: chem_comp / entity ...chem_comp / entity / entity_poly / pdbx_chem_comp_identifier / pdbx_entity_nonpoly / struct_conn / struct_site / struct_site_gen
Item: _chem_comp.name / _chem_comp.type ..._chem_comp.name / _chem_comp.type / _entity.pdbx_description / _entity_poly.pdbx_seq_one_letter_code_can / _pdbx_entity_nonpoly.name / _struct_conn.pdbx_role
Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 2.1Mar 24, 2021Group: Source and taxonomy / Structure summary / Category: chem_comp / entity_src_gen
Item: _chem_comp.pdbx_synonyms / _entity_src_gen.pdbx_host_org_cell_line / _entity_src_gen.pdbx_host_org_strain
Revision 2.2Oct 4, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
B: Light Chain of Fab C47B161
A: Heavy Chain of Fab C47B161
L: Light Chain of Fab C47B161
H: Heavy Chain of Fab C47B161
C: Leukocyte surface antigen CD47
D: Leukocyte surface antigen CD47
hetero molecules


Theoretical massNumber of molelcules
Total (without water)127,52314
Polymers126,3996
Non-polymers1,1248
Water2,126118
1
B: Light Chain of Fab C47B161
A: Heavy Chain of Fab C47B161
D: Leukocyte surface antigen CD47
hetero molecules


Theoretical massNumber of molelcules
Total (without water)63,8267
Polymers63,2003
Non-polymers6274
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
L: Light Chain of Fab C47B161
H: Heavy Chain of Fab C47B161
C: Leukocyte surface antigen CD47
hetero molecules


Theoretical massNumber of molelcules
Total (without water)63,6977
Polymers63,2003
Non-polymers4974
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)74.569, 60.974, 124.133
Angle α, β, γ (deg.)90.000, 90.050, 90.000
Int Tables number4
Space group name H-MP1211
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain D and (resseq 1:5 or (resid 6 and (name...
21(chain C and (resseq 1:9 or (resid 10 and (name...
12(chain B and (resseq 1:16 or (resid 17 and (name...
22(chain L and (resseq 1 or (resid 2 and (name...
13(chain A and (resseq 1:2 or (resid 3 and (name...
23(chain H and (resseq 1:2 or (resid 3 and (name...

NCS domain segments:
Dom-IDComponent-IDEns-IDSelection detailsAuth asym-IDAuth seq-ID
111(chain D and (resseq 1:5 or (resid 6 and (name...D1 - 5
121(chain D and (resseq 1:5 or (resid 6 and (name...D6
131(chain D and (resseq 1:5 or (resid 6 and (name...D1 - 216
141(chain D and (resseq 1:5 or (resid 6 and (name...D1 - 216
151(chain D and (resseq 1:5 or (resid 6 and (name...D1 - 216
161(chain D and (resseq 1:5 or (resid 6 and (name...D1 - 216
211(chain C and (resseq 1:9 or (resid 10 and (name...C1 - 9
221(chain C and (resseq 1:9 or (resid 10 and (name...C10
231(chain C and (resseq 1:9 or (resid 10 and (name...C1 - 293
241(chain C and (resseq 1:9 or (resid 10 and (name...C1 - 293
251(chain C and (resseq 1:9 or (resid 10 and (name...C1 - 293
261(chain C and (resseq 1:9 or (resid 10 and (name...C1 - 293
112(chain B and (resseq 1:16 or (resid 17 and (name...B1 - 16
122(chain B and (resseq 1:16 or (resid 17 and (name...B17
132(chain B and (resseq 1:16 or (resid 17 and (name...B1 - 218
142(chain B and (resseq 1:16 or (resid 17 and (name...B1 - 218
152(chain B and (resseq 1:16 or (resid 17 and (name...B1 - 218
162(chain B and (resseq 1:16 or (resid 17 and (name...B1 - 218
172(chain B and (resseq 1:16 or (resid 17 and (name...B1 - 218
182(chain B and (resseq 1:16 or (resid 17 and (name...B1 - 218
212(chain L and (resseq 1 or (resid 2 and (name...L1
222(chain L and (resseq 1 or (resid 2 and (name...L2
232(chain L and (resseq 1 or (resid 2 and (name...L1 - 219
242(chain L and (resseq 1 or (resid 2 and (name...L1 - 219
252(chain L and (resseq 1 or (resid 2 and (name...L1 - 219
262(chain L and (resseq 1 or (resid 2 and (name...L1 - 219
272(chain L and (resseq 1 or (resid 2 and (name...L1 - 219
282(chain L and (resseq 1 or (resid 2 and (name...L1 - 219
113(chain A and (resseq 1:2 or (resid 3 and (name...A1 - 2
123(chain A and (resseq 1:2 or (resid 3 and (name...A3
133(chain A and (resseq 1:2 or (resid 3 and (name...A1 - 217
143(chain A and (resseq 1:2 or (resid 3 and (name...A1 - 217
153(chain A and (resseq 1:2 or (resid 3 and (name...A1 - 217
163(chain A and (resseq 1:2 or (resid 3 and (name...A1 - 217
173(chain A and (resseq 1:2 or (resid 3 and (name...A1 - 217
183(chain A and (resseq 1:2 or (resid 3 and (name...A1 - 217
213(chain H and (resseq 1:2 or (resid 3 and (name...H1 - 2
223(chain H and (resseq 1:2 or (resid 3 and (name...H3
233(chain H and (resseq 1:2 or (resid 3 and (name...H1 - 217
243(chain H and (resseq 1:2 or (resid 3 and (name...H1 - 217
253(chain H and (resseq 1:2 or (resid 3 and (name...H1 - 217
263(chain H and (resseq 1:2 or (resid 3 and (name...H1 - 217
273(chain H and (resseq 1:2 or (resid 3 and (name...H1 - 217
283(chain H and (resseq 1:2 or (resid 3 and (name...H1 - 217

NCS ensembles :
ID
1
2
3

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Components

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Antibody , 3 types, 6 molecules BLAHCD

#1: Antibody Light Chain of Fab C47B161


Mass: 24140.988 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): HEK293 / Production host: Homo sapiens (human)
#2: Antibody Heavy Chain of Fab C47B161


Mass: 24311.193 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Cell line (production host): HEK293 / Production host: Homo sapiens (human)
#3: Antibody Leukocyte surface antigen CD47 / Antigenic surface determinant protein OA3 / Integrin-associated protein / IAP / Protein MER6


Mass: 14747.510 Da / Num. of mol.: 2 / Fragment: extracellular domain, UNP residues 19-141 / Mutation: C15G
Source method: isolated from a genetically manipulated source
Details: Residue 1 is a Gln that cyclizes as pyroglutamate (PCA)
Source: (gene. exp.) Homo sapiens (human) / Gene: CD47, MER6 / Cell line (production host): HEK293 / Production host: Homo sapiens (human) / References: UniProt: Q08722

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Sugars , 1 types, 3 molecules

#5: Sugar ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 2 types, 123 molecules

#4: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C3H8O3
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 118 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.23 Å3/Da / Density % sol: 44.92 % / Mosaicity: 1.408 °
Crystal growTemperature: 293.15 K / Method: vapor diffusion, sitting drop / pH: 7.5 / Details: 34% PEG 8000, 0.1M HEPES pH 7.5

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 17-ID / Wavelength: 1 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Nov 5, 2013
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.89→41.378 Å / Num. obs: 24865 / % possible obs: 98.6 % / Redundancy: 3.1 % / Biso Wilson estimate: 53.18 Å2 / Rmerge(I) obs: 0.119 / Rsym value: 0.119 / Net I/av σ(I): 9.093 / Net I/σ(I): 6.9
Reflection shellResolution: 2.89→2.95 Å / Redundancy: 2.9 % / Rmerge(I) obs: 0.465 / CC1/2: 0.763 / % possible all: 90.9

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Phasing

PhasingMethod: molecular replacement

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Processing

Software
NameVersionClassification
HKL-2000data reduction
PHASERphasing
PHENIX1.10_2155refinement
PDB_EXTRACT3.2data extraction
HKL-2000data scaling
DENZOdata reduction
SCALEPACKdata scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 2JJS

2jjs


Resolution: 2.89→41.378 Å / SU ML: 0.48 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 36.15
RfactorNum. reflection% reflectionSelection details
Rfree0.3279 1273 5.13 %Random selection
Rwork0.2573 ---
obs0.2609 24832 98.01 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 199.8 Å2 / Biso mean: 66.4767 Å2 / Biso min: 6.67 Å2
Refinement stepCycle: final / Resolution: 2.89→41.378 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms7962 0 72 118 8152
Biso mean--103.23 42.7 -
Num. residues----1096
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0058240
X-RAY DIFFRACTIONf_angle_d1.01611283
X-RAY DIFFRACTIONf_chiral_restr0.0621306
X-RAY DIFFRACTIONf_plane_restr0.0081453
X-RAY DIFFRACTIONf_dihedral_angle_d15.2134817
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11D789X-RAY DIFFRACTION12.506TORSIONAL
12C789X-RAY DIFFRACTION12.506TORSIONAL
21B1718X-RAY DIFFRACTION12.506TORSIONAL
22L1718X-RAY DIFFRACTION12.506TORSIONAL
31A1664X-RAY DIFFRACTION12.506TORSIONAL
32H1664X-RAY DIFFRACTION12.506TORSIONAL
LS refinement shellResolution: 2.8904→3.0061 Å
RfactorNum. reflection% reflection
Rfree0.3978 141 -
Rwork0.3218 2378 -
obs--91 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.75050.4106-1.75881.9907-1.48155.1489-0.0209-0.17330.1609-0.1752-0.0643-0.08710.01850.37370.04180.2607-0.00560.05620.2086-0.0380.3708-11.98654.161331.2664
29.789-1.54752.69945.9821-1.6785.8973-0.1519-0.8315-0.4342-0.82330.15820.2704-0.74-0.5362-0.17820.28280.0421-0.03840.2172-0.18790.1453-5.0847-4.959737.5325
30.7202-0.80140.10210.9298-0.69472.690.16620.15530.0293-0.25530.01380.1048-0.1422-0.2637-0.15940.5132-0.1566-0.02120.2027-0.00190.4804-8.7526-3.940243.5412
43.7047-1.09512.45520.5876-1.34983.0517-0.23710.0434-0.5263-0.20480.2752-0.2397-0.4191-0.14840.00320.77810.07480.15840.05270.00020.5954-6.4823-1.252967.2697
52.5449-1.05611.260.5855-0.58771.6153-0.5866-0.35780.25510.10140.2534-0.1436-0.24090.040.15620.50720.0287-0.0820.3227-0.01560.41691.42780.947278.8751
67.86360.7015.56830.67190.96427.14560.2816-0.769-1.06550.59740.0297-0.3290.7657-0.7567-0.41920.6275-0.34070.02180.156-0.00740.6136-10.7834-5.158177.0097
71.91670.1463.00525.92684.758.1938-0.11280.5277-0.2185-0.5467-0.5275-0.6860.04860.22730.06270.39090.09820.02240.41310.05440.162213.4509-6.199942.1334
83.57390.44492.36232.4533-1.0242.21970.3158-0.15590.10110.1541-0.0916-0.10520.54-0.1933-0.0930.4673-0.03430.16980.2129-0.0250.444910.3881-0.133531.7929
91.9671-2.4701-0.8826.17463.13852.30550.5125-0.45720.0337-1.106-0.0531-0.4887-0.30060.404-0.18040.7789-0.2140.21070.34670.03580.636117.9479-0.595331.5374
104.32490.91981.17380.6605-1.01613.82160.0778-0.0010.5685-0.12810.0550.6511-0.8295-0.2137-0.19990.5104-0.01060.25290.352-0.02470.466510.64664.849539.8247
111.0354-0.00550.51070.7272-1.2084.22020.3737-0.2003-0.32310.2317-0.35280.2501-0.00140.0094-0.11570.30170.0765-0.110.0874-0.06830.53226.7082-5.471556.0839
121.01392.1609-1.41446.1565-2.98432.699-0.0575-0.29030.10630.04090.0337-0.4330.13090.05760.1360.31920.06030.08690.27120.00180.41667.2901-10.131361.6389
133.1288-0.44412.25691.4973-0.54596.2536-0.17960.0483-0.38880.6813-0.18530.13950.7157-0.27010.31970.5252-0.03660.10690.2032-0.04330.5231-48.1326-31.466726.8445
144.30830.7489-2.30692.34480.72345.03450.09630.132-0.10930.06790.0560.19510.712-0.0412-0.07140.441-0.0517-0.14690.1150.00260.3767-40.7819-30.2457-10.6545
151.53112.35950.26393.87161.31852.35730.35510.1140.32020.1046-0.1649-0.38310.39330.133-0.04190.35550.0759-0.06540.2534-0.00270.4185-24.6349-28.179925.7582
160.50.0376-0.05921.1933-0.14870.517-0.10640.0609-0.72040.21190.01690.1985-0.099-0.0932-0.63090.6115-0.23320.62110.00080.04580.7183-31.8946-37.022828.4679
172.5715-0.20851.29230.1412-0.48114.14140.5552-0.0641-0.85441.06630.4821-0.85610.0811-0.3829-0.39760.4910.15480.01850.22830.15690.6976-24.9828-35.709636.4845
180.7577-1.9124-1.09048.25984.97293.70260.202-0.2948-0.2890.94560.1289-0.71610.74870.4498-0.19890.4910.0826-0.24160.4152-0.06160.7429-19.0776-31.986230.2363
190.71780.25360.22641.1692-1.45314.1613-0.11510.0510.03820.1327-0.047-0.1640.16110.22530.23550.39290.0026-0.02990.1647-0.01670.5664-29.6924-29.321110.1229
200.7005-0.6489-0.18454.7299-1.45171.37570.1567-0.4054-0.28580.8495-0.7885-0.3312-0.15360.45270.29190.40990.13450.1853-0.3261-0.03150.8129-29.8343-22.28120.3778
215.1907-1.8285-3.25441.18620.15374.63350.2967-0.58050.87360.3941-0.16920.4182-0.37370.2536-0.03710.9418-0.15590.09430.4891-0.15540.6322-40.0939-17.849355.0266
225.0152.7923-4.58722.4248-3.45485.13070.02610.72460.65691.2116-0.0999-0.2089-0.2229-0.7994-0.13961.1137-0.2475-0.02120.7595-0.10290.5597-52.9896-19.552654.8169
235.9255-0.962-0.67651.45022.6386.41430.3665-0.78390.51421.4146-0.33481.08480.1510.1335-0.12021.0466-0.29550.25690.49470.01870.6706-41.5443-18.437956.3402
241.2379-0.2727-0.18760.99492.48086.39140.00640.5773-0.99860.12510.1850.15891.83810.3430.02362.00230.22120.4060.8114-0.16050.82862.5966-16.176-1.0836
254.73571.73413.54924.7265-0.17115.44060.6991-0.0611-0.03890.072-0.09870.62510.129-0.9641-0.44740.54440.06060.03910.55410.04550.5442-7.5308-15.41899.649
262.3695-0.1207-1.66931.86121.27152.76990.43660.90430.4348-0.6751-0.4650.21190.02370.1755-0.28960.89720.50630.14970.8281-0.07030.4543-4.7487-14.42843.2549
271.3231-0.2057-0.64332.19411.52811.230.1822-0.0230.8222-0.27630.72440.0736-0.8749-0.0758-0.62910.64710.10550.10170.47870.06010.46771.3287-10.65524.5149
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'B' and (resid 1 through 37 )B1 - 37
2X-RAY DIFFRACTION2chain 'B' and (resid 38 through 53 )B38 - 53
3X-RAY DIFFRACTION3chain 'B' and (resid 54 through 133 )B54 - 133
4X-RAY DIFFRACTION4chain 'B' and (resid 134 through 179 )B134 - 179
5X-RAY DIFFRACTION5chain 'B' and (resid 180 through 202 )B180 - 202
6X-RAY DIFFRACTION6chain 'B' and (resid 203 through 218 )B203 - 218
7X-RAY DIFFRACTION7chain 'A' and (resid 2 through 12 )A2 - 12
8X-RAY DIFFRACTION8chain 'A' and (resid 13 through 64 )A13 - 64
9X-RAY DIFFRACTION9chain 'A' and (resid 65 through 83 )A65 - 83
10X-RAY DIFFRACTION10chain 'A' and (resid 84 through 99 )A84 - 99
11X-RAY DIFFRACTION11chain 'A' and (resid 100 through 149 )A100 - 149
12X-RAY DIFFRACTION12chain 'A' and (resid 150 through 217 )A150 - 217
13X-RAY DIFFRACTION13chain 'L' and (resid 1 through 118 )L1 - 118
14X-RAY DIFFRACTION14chain 'L' and (resid 119 through 219 )L119 - 219
15X-RAY DIFFRACTION15chain 'H' and (resid 2 through 39 )H2 - 39
16X-RAY DIFFRACTION16chain 'H' and (resid 40 through 52 )H40 - 52
17X-RAY DIFFRACTION17chain 'H' and (resid 53 through 64 )H53 - 64
18X-RAY DIFFRACTION18chain 'H' and (resid 65 through 84 )H65 - 84
19X-RAY DIFFRACTION19chain 'H' and (resid 85 through 149 )H85 - 149
20X-RAY DIFFRACTION20chain 'H' and (resid 150 through 217 )H150 - 217
21X-RAY DIFFRACTION21chain 'C' and (resid 2 through 51 )C2 - 51
22X-RAY DIFFRACTION22chain 'C' and (resid 52 through 63 )C52 - 63
23X-RAY DIFFRACTION23chain 'C' and (resid 64 through 111 )C64 - 111
24X-RAY DIFFRACTION24chain 'D' and (resid 2 through 16 )D2 - 16
25X-RAY DIFFRACTION25chain 'D' and (resid 17 through 72 )D17 - 72
26X-RAY DIFFRACTION26chain 'D' and (resid 73 through 100 )D73 - 100
27X-RAY DIFFRACTION27chain 'D' and (resid 101 through 116 )D101 - 116

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