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- PDB-5t01: Human c-Jun DNA binding domain homodimer in complex with methylat... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5t01 | ||||||
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Title | Human c-Jun DNA binding domain homodimer in complex with methylated DNA | ||||||
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![]() | TRANSCRIPTION REGULATOR/DNA / Zta / Zebra / BZLF-1 / AP-1 / Epstein-Barr virus / EBV / 5-methylcytosine / 5mC / DNA methylation / transcription factor / basic leucine-zipper / bZIP / TRANSCRIPTION REGULATOR-DNA complex | ||||||
Function / homology | ![]() cAMP response element binding / transcription factor AP-1 complex / integrated stress response signaling / positive regulation of DNA-templated transcription initiation / release from viral latency / WNT5:FZD7-mediated leishmania damping / SMAD protein signal transduction / positive regulation by host of viral transcription / nuclear chromosome / Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models ...cAMP response element binding / transcription factor AP-1 complex / integrated stress response signaling / positive regulation of DNA-templated transcription initiation / release from viral latency / WNT5:FZD7-mediated leishmania damping / SMAD protein signal transduction / positive regulation by host of viral transcription / nuclear chromosome / Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models / negative regulation of DNA binding / Activation of the AP-1 family of transcription factors / ubiquitin-like protein ligase binding / R-SMAD binding / general transcription initiation factor binding / negative regulation by host of viral transcription / positive regulation of vascular associated smooth muscle cell proliferation / cellular response to cadmium ion / response to endoplasmic reticulum stress / GTPase activator activity / transforming growth factor beta receptor signaling pathway / Regulation of PTEN gene transcription / TP53 Regulates Transcription of DNA Repair Genes / FCERI mediated MAPK activation / MAPK6/MAPK4 signaling / euchromatin / Pre-NOTCH Transcription and Translation / DNA-binding transcription repressor activity, RNA polymerase II-specific / positive regulation of miRNA transcription / Activation of anterior HOX genes in hindbrain development during early embryogenesis / cellular response to reactive oxygen species / RNA polymerase II transcription regulator complex / sequence-specific double-stranded DNA binding / Signaling by ALK fusions and activated point mutants / regulation of cell population proliferation / Senescence-Associated Secretory Phenotype (SASP) / DNA-binding transcription activator activity, RNA polymerase II-specific / Oxidative Stress Induced Senescence / Estrogen-dependent gene expression / RNA polymerase II-specific DNA-binding transcription factor binding / transcription regulator complex / transcription cis-regulatory region binding / DNA-binding transcription factor activity, RNA polymerase II-specific / regulation of cell cycle / positive regulation of apoptotic process / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of DNA-templated transcription / ubiquitin protein ligase binding / chromatin / regulation of transcription by RNA polymerase II / positive regulation of DNA-templated transcription / enzyme binding / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / RNA binding / nucleoplasm / identical protein binding / nucleus Similarity search - Function | ||||||
Biological species | ![]() synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Hong, S. / Horton, J.R. / Cheng, X. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Methyl-dependent and spatial-specific DNA recognition by the orthologous transcription factors human AP-1 and Epstein-Barr virus Zta. Authors: Hong, S. / Wang, D. / Horton, J.R. / Zhang, X. / Speck, S.H. / Blumenthal, R.M. / Cheng, X. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 110.2 KB | Display | ![]() |
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PDB format | ![]() | 82.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 445.7 KB | Display | ![]() |
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Full document | ![]() | 447.2 KB | Display | |
Data in XML | ![]() | 9.7 KB | Display | |
Data in CIF | ![]() | 13.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5szxC ![]() 1fosS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: DNA chain | Mass: 5706.697 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) | ||
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#2: DNA chain | Mass: 5956.901 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) | ||
#3: Protein | Mass: 7591.038 Da / Num. of mol.: 2 / Fragment: DNA binding domain (UNP residues 254-315) / Mutation: C269S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.81 Å3/Da / Density % sol: 56.25 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 5 Details: 0.05 M Citric acid 0.05 M Bis-Tris propane 16% PEG3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX300-HS / Detector: CCD / Date: Feb 27, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.89→35 Å / Num. obs: 23735 / % possible obs: 98.5 % / Redundancy: 6.9 % / Rmerge(I) obs: 0.083 / Net I/σ(I): 20.3 |
Reflection shell | Resolution: 1.89→1.96 Å / Redundancy: 4.2 % / Rmerge(I) obs: 0.8 / Mean I/σ(I) obs: 2.63 / % possible all: 90.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1FOS Resolution: 1.89→27.75 Å / SU ML: 0.24 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 28.09 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.89→27.75 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: -24.4193 Å / Origin y: 16.7252 Å / Origin z: 20.1421 Å
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Refinement TLS group | Selection details: all |