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Open data
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Basic information
Entry | Database: PDB / ID: 5suq | ||||||
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Title | Crystal structure of the THO-Sub2 complex | ||||||
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![]() | HYDROLASE / mRNA export | ||||||
Function / homology | ![]() transcription export complex / mRNA 3'-end processing / subtelomeric heterochromatin formation / transcription-coupled nucleotide-excision repair / mRNA export from nucleus / transcription elongation by RNA polymerase II / spliceosomal complex / mRNA splicing, via spliceosome / chromosome, telomeric region / RNA helicase activity ...transcription export complex / mRNA 3'-end processing / subtelomeric heterochromatin formation / transcription-coupled nucleotide-excision repair / mRNA export from nucleus / transcription elongation by RNA polymerase II / spliceosomal complex / mRNA splicing, via spliceosome / chromosome, telomeric region / RNA helicase activity / RNA helicase / mRNA binding / ATP hydrolysis activity / RNA binding / ATP binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Ren, Y. / Blobel, G. | ||||||
![]() | ![]() Title: Structural and biochemical analyses of the DEAD-box ATPase Sub2 in association with THO or Yra1. Authors: Ren, Y. / Schmiege, P. / Blobel, G. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 661.8 KB | Display | ![]() |
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PDB format | ![]() | 507.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 4.4 MB | Display | ![]() |
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Full document | ![]() | 4.5 MB | Display | |
Data in XML | ![]() | 91.2 KB | Display | |
Data in CIF | ![]() | 153.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5supSC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 50375.859 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: ATCC 204508 / S288c / Gene: SUB2, YDL084W / Production host: ![]() ![]() #2: Protein | Mass: 34059.902 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() #3: Protein | Mass: 195762.641 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() #4: Chemical | ChemComp-KEG / Compound details | THE DEPOSITORS STATE THAT BECAUSE OF THE 6.0 A RESOLUTION OF THE DATA AND LACK OF STRUCTURAL ...THE DEPOSITORS | Sequence details | CHAINS B AND D ARE TWO MOLECULES OF TEX1 PROTEIN FROM SACCHAROMYCES BAYANUS. CHAINS M AND N EACH ...CHAINS B AND D ARE TWO MOLECULES OF TEX1 PROTEIN FROM SACCHAROMY | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal grow | Temperature: 289 K / Method: evaporation / pH: 8.4 Details: 100 mM Tris-HCl (pH 8.4), 17.5% PEG3350, 2% methanol, 0.3 M NDSB-195 |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Aug 15, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.2123 Å / Relative weight: 1 |
Reflection | Resolution: 6→50 Å / Num. obs: 41038 / % possible obs: 98.8 % / Redundancy: 6.5 % / CC1/2: 0.993 / Rmerge(I) obs: 0.14 / Net I/σ(I): 13.3 |
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Processing
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Refinement | Starting model: 5SUP Resolution: 6→49.57 Å / SU ML: 1.53 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 53.87
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Solvent computation | Shrinkage radii: 1.2 Å / VDW probe radii: 1.4 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 6→49.57 Å
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Refine LS restraints |
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LS refinement shell |
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