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- EMDB-11363: Cryo-EM structure of the 90S pre-ribosome from Saccharomyces cere... -

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Basic information

Entry
Database: EMDB / ID: EMD-11363
TitleCryo-EM structure of the 90S pre-ribosome from Saccharomyces cerevisiae, state Dis-C
Map dataMultibody refine focus 3domain
Sample
  • Complex: 90S pre-ribbosome state Dis-C
    • Protein or peptide: x 33 types
    • RNA: x 3 types
  • Ligand: x 3 types
Keywordsribosome / 90S pre-ribosome / 40S pre-ribosome / A1 cleavage / Dhr1
Function / homology
Function and homology information


18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase / 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity / rRNA small subunit pseudouridine methyltransferase Nep1 / Noc4p-Nop14p complex / nuclear microtubule / Mpp10 complex / rRNA (pseudouridine) methyltransferase activity / rRNA modification / regulation of rRNA processing / rRNA (adenine-N6,N6-)-dimethyltransferase activity ...18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase / 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity / rRNA small subunit pseudouridine methyltransferase Nep1 / Noc4p-Nop14p complex / nuclear microtubule / Mpp10 complex / rRNA (pseudouridine) methyltransferase activity / rRNA modification / regulation of rRNA processing / rRNA (adenine-N6,N6-)-dimethyltransferase activity / RNA folding chaperone / endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / single-stranded telomeric DNA binding / rRNA primary transcript binding / sno(s)RNA-containing ribonucleoprotein complex / Negative regulators of DDX58/IFIH1 signaling / rRNA base methylation / mTORC1-mediated signalling / rRNA methylation / Protein hydroxylation / U3 snoRNA binding / positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay / poly(A)+ mRNA export from nucleus / Formation of the ternary complex, and subsequently, the 43S complex / Translation initiation complex formation / Ribosomal scanning and start codon recognition / snoRNA binding / preribosome, small subunit precursor / Major pathway of rRNA processing in the nucleolus and cytosol / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / Formation of a pool of free 40S subunits / L13a-mediated translational silencing of Ceruloplasmin expression / 90S preribosome / Ub-specific processing proteases / proteasome assembly / ribosomal subunit export from nucleus / regulation of translational fidelity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal small subunit export from nucleus / RNA endonuclease activity / nuclear periphery / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosome assembly / maturation of SSU-rRNA / small-subunit processome / translational initiation / enzyme activator activity / helicase activity / maintenance of translational fidelity / rRNA processing / unfolded protein binding / ribosome biogenesis / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / cytoplasmic translation / RNA helicase activity / rRNA binding / structural constituent of ribosome / RNA helicase / ribosome / translation / GTPase activity / mRNA binding / GTP binding / nucleolus / ATP hydrolysis activity / mitochondrion / RNA binding / zinc ion binding / nucleoplasm / ATP binding / identical protein binding / nucleus / cytoplasm / cytosol
Similarity search - Function
Nucleolar protein 14 / Nop14-like family / rRNA-processing protein Fcf1, PIN domain / Small-subunit processome, Utp14 / Utp14 protein / rRNA-processing protein Fcf1/Utp23 / Sas10 C-terminal domain / Fcf1 / Sas10 C-terminal domain / RNA 3'-terminal phosphate cyclase-like, conserved site ...Nucleolar protein 14 / Nop14-like family / rRNA-processing protein Fcf1, PIN domain / Small-subunit processome, Utp14 / Utp14 protein / rRNA-processing protein Fcf1/Utp23 / Sas10 C-terminal domain / Fcf1 / Sas10 C-terminal domain / RNA 3'-terminal phosphate cyclase-like, conserved site / Ribosome biogenesis protein Bms1, N-terminal / RNA 3'-terminal phosphate cyclase signature. / Sas10/Utp3/C1D / U3 small nucleolar ribonucleoprotein complex, subunit Mpp10 / RNA 3'-terminal phosphate cyclase type 2 / Sas10/Utp3/C1D family / Mpp10 protein / RNA 3'-terminal phosphate cyclase / Ribosomal biogenesis, methyltransferase, EMG1/NEP1 / RNA 3'-terminal phosphate cyclase, insert domain / RNA 3'-terminal phosphate cyclase domain / RNA 3'-terminal phosphate cyclase, insert domain superfamily / RNA 3'-terminal phosphate cyclase domain superfamily / RNA 3'-terminal phosphate cyclase / EMG1/NEP1 methyltransferase / RNA 3'-terminal phosphate cyclase (RTC), insert domain / Bystin / Krr1, KH1 domain / Bystin / Krr1 KH1 domain / Ribosomal RNA adenine dimethylase / Ribosomal RNA adenine methylase transferase, conserved site / Ribosomal RNA adenine dimethylases signature. / : / : / Eukaryotic type KH-domain (KH-domain type I) / U3 snoRNP protein/Ribosome production factor 1 / Ribosomal RNA adenine methylase transferase, N-terminal / Ribosomal RNA adenine dimethylases / Large family of predicted nucleotide-binding domains / Ribosome biogenesis protein BMS1/TSR1, C-terminal / AARP2CN / Bms1/Tsr1-type G domain / Ribosome biogenesis protein Bms1/Tsr1 / 40S ribosome biogenesis protein Tsr1 and BMS1 C-terminal / AARP2CN (NUC121) domain / Bms1-type guanine nucleotide-binding (G) domain profile. / AARP2CN (NUC121) domain / Protein of unknown function (DUF663) / Ribosomal RNA adenine methyltransferase KsgA/Erm / Ribosomal RNA adenine dimethylase / rRNA adenine N(6)-methyltransferase family profile. / PIN domain / : / Brix domain / Brix domain / Helicase associated domain (HA2), ratchet-like / Brix domain profile. / Brix / DEAD-box helicase, OB fold / RNA 3'-terminal phosphate cyclase/enolpyruvate transferase, alpha/beta / Oligonucleotide/oligosaccharide-binding (OB)-fold / Helicase associated domain (HA2), winged-helix / Helicase-associated domain / Helicase associated domain (HA2) Add an annotation / tRNA (guanine-N1-)-methyltransferase, N-terminal / Alpha/beta knot methyltransferases / K Homology domain, type 1 superfamily / PIN-like domain superfamily / DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site / DEAH-box subfamily ATP-dependent helicases signature. / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / : / Ribosomal protein S7e signature. / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein S3Ae, conserved site / Ribosomal protein S3Ae signature. / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein S27e signature. / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein S4e signature. / 40S ribosomal protein S4, C-terminal domain / 40S ribosomal protein S4 C-terminus / Ribosomal protein S8e, conserved site / Ribosomal protein S8e signature. / Ribosomal protein S19e / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S6, eukaryotic / Ribosomal protein S7e / Ribosomal protein S7e / 40S ribosomal protein S1/3, eukaryotes / 40S ribosomal protein S11, N-terminal / Ribosomal_S17 N-terminal / Ribosomal S24e conserved site / Ribosomal protein S24e signature. / : / Ribosomal protein S4e, N-terminal
Similarity search - Domain/homology
Small ribosomal subunit protein uS4A / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS11A / Small ribosomal subunit protein eS19A / Small ribosomal subunit protein uS8A / Small ribosomal subunit protein uS12A / Small ribosomal subunit protein eS24A / Small ribosomal subunit protein eS4A / Small ribosomal subunit protein eS6A / Small ribosomal subunit protein eS8A ...Small ribosomal subunit protein uS4A / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS11A / Small ribosomal subunit protein eS19A / Small ribosomal subunit protein uS8A / Small ribosomal subunit protein uS12A / Small ribosomal subunit protein eS24A / Small ribosomal subunit protein eS4A / Small ribosomal subunit protein eS6A / Small ribosomal subunit protein eS8A / Small ribosomal subunit protein uS17A / Small ribosomal subunit protein uS9A / Small ribosomal subunit protein uS13A / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein eS7A / Small ribosomal subunit protein eS1A / Small ribosomal subunit protein eS27A / Essential nuclear protein 1 / Dimethyladenosine transferase / U3 small nucleolar RNA-associated protein MPP10 / U3 small nucleolar ribonucleoprotein protein IMP4 / Probable ATP-dependent RNA helicase DHR1 / U3 small nucleolar RNA-associated protein 14 / rRNA-processing protein FCF1 / Ribosomal RNA small subunit methyltransferase NEP1 / rRNA processing protein RCL1 / Ribosome biogenesis protein BMS1 / Something about silencing protein 10 / Small ribosomal subunit protein eS25A / Small ribosomal subunit protein eS28A / Nucleolar complex protein 14 / Pre-rRNA-processing protein PNO1
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast) / Saccharomyces cerevisiae S288C (yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.5 Å
AuthorsCheng J / Lau B
CitationJournal: Science / Year: 2020
Title: 90 pre-ribosome transformation into the primordial 40 subunit.
Authors: Jingdong Cheng / Benjamin Lau / Giuseppe La Venuta / Michael Ameismeier / Otto Berninghausen / Ed Hurt / Roland Beckmann /
Abstract: Production of small ribosomal subunits initially requires the formation of a 90 precursor followed by an enigmatic process of restructuring into the primordial pre-40 subunit. We elucidate this ...Production of small ribosomal subunits initially requires the formation of a 90 precursor followed by an enigmatic process of restructuring into the primordial pre-40 subunit. We elucidate this process by biochemical and cryo-electron microscopy analysis of intermediates along this pathway in yeast. First, the remodeling RNA helicase Dhr1 engages the 90 pre-ribosome, followed by Utp24 endonuclease-driven RNA cleavage at site A, thereby separating the 5'-external transcribed spacer (ETS) from 18 ribosomal RNA. Next, the 5'-ETS and 90 assembly factors become dislodged, but this occurs sequentially, not en bloc. Eventually, the primordial pre-40 emerges, still retaining some 90 factors including Dhr1, now ready to unwind the final small nucleolar U3-18 RNA hybrid. Our data shed light on the elusive 90 to pre-40 transition and clarify the principles of assembly and remodeling of large ribonucleoproteins.
History
DepositionJul 9, 2020-
Header (metadata) releaseSep 23, 2020-
Map releaseSep 23, 2020-
UpdateMar 6, 2024-
Current statusMar 6, 2024Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.01
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  • Surface view colored by radius
  • Surface level: 0.01
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  • Surface view with fitted model
  • Atomic models: PDB-6zqg
  • Surface level: 0.01
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Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_11363.png

Downloads & links

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Map

FileDownload / File: emd_11363.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationMultibody refine focus 3domain
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.06 Å/pix.
x 480 pix.
= 508.32 Å		1.06 Å/pix.
x 480 pix.
= 508.32 Å		1.06 Å/pix.
x 480 pix.
= 508.32 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.059 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.01 / Movie #1: 0.01
Minimum - Maximum-0.043471146 - 0.09912797
Average (Standard dev.)0.0001424978 (±0.0018802981)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 508.32 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.0591.0591.059
M x/y/z480480480
origin x/y/z0.0000.0000.000
length x/y/z508.320508.320508.320
α/β/γ90.00090.00090.000
start NX/NY/NZ000
NX/NY/NZ100115122
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS480480480
D min/max/mean-0.0430.0990.000

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Supplemental data

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Additional map: Multibody refine focus 3domain

Fileemd_11363_additional.map
AnnotationMultibody refine focus 3domain
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : 90S pre-ribbosome state Dis-C

EntireName: 90S pre-ribbosome state Dis-C
Components
  • Complex: 90S pre-ribbosome state Dis-C
    • Protein or peptide: Nucleolar complex protein 14
    • Protein or peptide: Something about silencing protein 10
    • Protein or peptide: Noc4,Nucleolar complex protein 4,Noc4
    • Protein or peptide: rRNA-processing protein FCF1
    • Protein or peptide: U3 small nucleolar ribonucleoprotein protein IMP4
    • Protein or peptide: U3 small nucleolar RNA-associated protein MPP10
    • Protein or peptide: Ribosome biogenesis protein BMS1
    • Protein or peptide: RNA 3'-terminal phosphate cyclase-like protein
    • Protein or peptide: Ribosomal RNA small subunit methyltransferase NEP1
    • Protein or peptide: Essential nuclear protein 1
    • Protein or peptide: Dimethyladenosine transferase
    • Protein or peptide: Pre-rRNA-processing protein PNO1
    • Protein or peptide: Rps5p
    • Protein or peptide: 40S ribosomal protein S16-A
    • Protein or peptide: 40S ribosomal protein S18-A,40S ribosomal protein S18-A,Rps18
    • Protein or peptide: 40S ribosomal protein S19-A
    • Protein or peptide: 40S ribosomal protein S28-A
    • RNA: 18S rRNA
    • Protein or peptide: 40S ribosomal protein S1-A
    • Protein or peptide: 40S ribosomal protein S4-A
    • Protein or peptide: 40S ribosomal protein S6-A
    • Protein or peptide: 40S ribosomal protein S7-A
    • Protein or peptide: 40S ribosomal protein S8-A
    • Protein or peptide: 40S ribosomal protein S9-A
    • Protein or peptide: 40S ribosomal protein S11-A
    • Protein or peptide: 40S ribosomal protein S13
    • Protein or peptide: 40S ribosomal protein S14-A
    • Protein or peptide: 40S ribosomal protein S25-A
    • Protein or peptide: 40S ribosomal protein S22-A
    • Protein or peptide: 40S ribosomal protein S23-A
    • Protein or peptide: 40S ribosomal protein S24-A
    • Protein or peptide: 40S ribosomal protein S27-A
    • Protein or peptide: U3 small nucleolar RNA-associated protein 14
    • Protein or peptide: Probable ATP-dependent RNA helicase DHR1
    • RNA: U3 snoRNA
    • RNA: Poly-U RNA
  • Ligand: ZINC ION
  • Ligand: GUANOSINE-5'-TRIPHOSPHATE
  • Ligand: MAGNESIUM ION

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Supramolecule #1: 90S pre-ribbosome state Dis-C

SupramoleculeName: 90S pre-ribbosome state Dis-C / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#36
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)

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Macromolecule #1: Nucleolar complex protein 14

MacromoleculeName: Nucleolar complex protein 14 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 94.463195 KDa
SequenceString: MAGSQLKNLK AALKARGLTG QTNVKSKNKK NSKRQAKEYD REEKKKAIAE IREEFNPFEI KAARNKRRDG LPSKTADRIA VGKPGISKQ IGEEQRKRAF EARKMMKNKR GGVIDKRFGE RDKLLTEEEK MLERFTRERQ SQSKRNANLF NLEDDEDDGD M FGDGLTHL ...String:
MAGSQLKNLK AALKARGLTG QTNVKSKNKK NSKRQAKEYD REEKKKAIAE IREEFNPFEI KAARNKRRDG LPSKTADRIA VGKPGISKQ IGEEQRKRAF EARKMMKNKR GGVIDKRFGE RDKLLTEEEK MLERFTRERQ SQSKRNANLF NLEDDEDDGD M FGDGLTHL GQSLSLEDEL ANDEEDFLAS KRFNEDDAEL QQPQRKKTKA EVMKEVIAKS KFYKQERQKA QGIMEDQIDN LD DNFEDVM SELMMTQPKK NPMEPKTDLD KEYDIKVKEL QLDKRAAPSD RTKTEEEKNA EAEEKKRELE QQRLDRMNGM IEL EEGEER GVEDLDDGFW ENEEDYEDDN DGIADSDDDI KFEDQGRDEG FSQILKKKNI SISCPRTHDA LLDQVKKLDL DDHP KIVKN IIKAYQPKLA EGNKEKLGKF TAVLLRHIIF LSNQNYLKNV QSFKRTQNAL ISILKSLSEK YNRELSEECR DYINE MQAR YKKNHFDALS NGDLVFFSII GILFSTSDQY HLVITPALIL MSQFLEQIKF NSLKRIAFGA VLVRIVSQYQ RISKRY IPE VVYFFQKILL TFIVEKENQE KPLDFENIRL DSYELGLPLD VDFTKKRSTI IPLHTLSTMD TEAHPVDQCV SVLLNVM ES LDATISTVWK SLPAFNEIIL PIQQLLSAYT SKYSDFEKPR NILNKVEKLT KFTEHIPLAL QNHKPVSIPT HAPKYEEN F NPDKKSYDPD RTRSEINKMK AQLKKERKFT MKEIRKDAKF EARQRIEEKN KESSDYHAKM AHIVNTINTE EGAEKNKYE RERKLRGGKK

UniProtKB: Nucleolar complex protein 14

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Macromolecule #2: Something about silencing protein 10

MacromoleculeName: Something about silencing protein 10 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 70.364398 KDa
SequenceString: MVRKGSNRTK TSEVGDEINP YGLNEVDDFA SKREKVLLGQ STFGDSNKDD DHSLLEDEDE EEVLAMDEDD ESIDEREDEE EEEEEELDG AAAYKKIFGR NLETDQLPEE DEENGMLDNE NAWGSTKGEY YGADDLDDDE AAKEIEKEAL RQQKKHLEEL N MNDYLDEE ...String:
MVRKGSNRTK TSEVGDEINP YGLNEVDDFA SKREKVLLGQ STFGDSNKDD DHSLLEDEDE EEVLAMDEDD ESIDEREDEE EEEEEELDG AAAYKKIFGR NLETDQLPEE DEENGMLDNE NAWGSTKGEY YGADDLDDDE AAKEIEKEAL RQQKKHLEEL N MNDYLDEE EEEEWVKSAK EFDMGEFKNS TKQADTKTSI TDILNMDDEA RDNYLRTMFP EFAPLSKEFT ELAPKFDELK KS EENEFNK LKLIALGSYL GTISCYYSIL LHELHNNEDF TSMKGHPVME KILTTKEIWR QASELPSSFD VNEGDGSESE ETA NIEAFN EKKLNELQNS EDSDAEDGGK QKQEIDEEER ESDEEEEEED VDIDDFEEYV AQSRLHSKPK TSSMPEADDF IESE IADVD AQDKKARRRT LRFYTSKIDQ QENKKTDRFK GDDDIPYKER LFERQQRLLD EARKRGMHDN NGADLDDKDY GSEDE AVSR SINTQGENDY YQQVQRGKQD KKISRKEAHK NAVIAAREGK LAELAENVSG DGKRAINYQI LKNKGLTPKR NKDNRN SRV KKRKKYQKAQ KKLKSVRAVY SGGQSGVYEG EKTGIKKGLT RSVKFKN

UniProtKB: Something about silencing protein 10

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Macromolecule #3: Noc4,Nucleolar complex protein 4,Noc4

MacromoleculeName: Noc4,Nucleolar complex protein 4,Noc4 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 63.707844 KDa
SequenceString: MVLLISEIKD IAKRLTAAGD RKQYNSIIKL INELVIPENV TQLEEDETEK NLRFLVMSLF QIFRKLFSRG DLTLPSSKKS TLEKEQFVN WCRKVYEAFK TKLLAIISDI PFETSLGLDS LDVYLQLAEL ESTHFASEKG APFFPNKTFR KLIIALWSSN M GEIEDVKS ...String:
MVLLISEIKD IAKRLTAAGD RKQYNSIIKL INELVIPENV TQLEEDETEK NLRFLVMSLF QIFRKLFSRG DLTLPSSKKS TLEKEQFVN WCRKVYEAFK TKLLAIISDI PFETSLGLDS LDVYLQLAEL ESTHFASEKG APFFPNKTFR KLIIALWSSN M GEIEDVKS SGASENLIIV EFTEKYYTKF ADIQYYFQSE FNQLLEDPAY QDLLLKNVGK WLALVNHDKH CSSVDADLEI FV PNPPQAI ENESKFKSNF EKNWLSLLNG QLSLQQYKSI LLILHKRIIP HFHTPTKLMD FLTDSYNLQS SNKNAGVVPI LAL NGLFEL MKRFNLEYPN FYMKLYQIIN PDLMHVKYRA RFFRLMDVFL SSTHLSAHLV ASFIKKLARL TLESPPSAIV TVIP FIYNL IRKHPNCMIM LHNPAFISNP FQTPDQVANL KTLKENYVDP FDVHESDPEL THALDSSLWE LASLMEHYHP NVATL AKIF AQPFKKLSYN MEDFLDWNYD SLLNAESSRK LKTLPTLEFE AFTNVFDNED GDSEASSQGN VYLPGVAW

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Macromolecule #4: rRNA-processing protein FCF1

MacromoleculeName: rRNA-processing protein FCF1 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 21.650729 KDa
SequenceString:
MGKAKKTRKF GLVKRTLNTK KDQRLKKNQE NIKTKEDPEL TRNIPQVSSA LFFQYNQAIK PPYQVLIDTN FINFSIQKKV DIVRGMMDC LLAKCNPLIT DCVMAELEKL GPKYRIALKL ARDPRIKRLS CSHKGTYADD CLVHRVLQHK CYIVATNDAG L KQRIRKIP GIPLMSVGGH AYVIEKLPDV F

UniProtKB: rRNA-processing protein FCF1

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Macromolecule #5: U3 small nucleolar ribonucleoprotein protein IMP4

MacromoleculeName: U3 small nucleolar ribonucleoprotein protein IMP4 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 33.536168 KDa
SequenceString: MLRRQARERR EYLYRKAQEL QDSQLQQKRQ IIKQALAQGK PLPKELAEDE SLQKDFRYDQ SLKESEEADD LQVDDEYAAT SGIMDPRII VTTSRDPSTR LSQFAKEIKL LFPNAVRLNR GNYVMPNLVD ACKKSGTTDL VVLHEHRGVP TSLTISHFPH G PTAQFSLH ...String:
MLRRQARERR EYLYRKAQEL QDSQLQQKRQ IIKQALAQGK PLPKELAEDE SLQKDFRYDQ SLKESEEADD LQVDDEYAAT SGIMDPRII VTTSRDPSTR LSQFAKEIKL LFPNAVRLNR GNYVMPNLVD ACKKSGTTDL VVLHEHRGVP TSLTISHFPH G PTAQFSLH NVVMRHDIIN AGNQSEVNPH LIFDNFTTAL GKRVVCILKH LFNAGPKKDS ERVITFANRG DFISVRQHVY VR TREGVEI AEVGPRFEMR LFELRLGTLE NKDADVEWQL RRFIRTANKK DYL

UniProtKB: U3 small nucleolar ribonucleoprotein protein IMP4

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Macromolecule #6: U3 small nucleolar RNA-associated protein MPP10

MacromoleculeName: U3 small nucleolar RNA-associated protein MPP10 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 67.042492 KDa
SequenceString: MSELFGVLKS NAGRIILKDP SATSKDVKAY IDSVINTCKN GSITKKAELD EITVDGLDAN QVWWQVKLVL DSIDGDLIQG IQELKDVVT PSHNLSDGST LNSSSGEESE LEEAESVFKE KQMLSADVSE IEEQSNDSLS ENDEEPSMDD EKTSAEAARE E FAEEKRIS ...String:
MSELFGVLKS NAGRIILKDP SATSKDVKAY IDSVINTCKN GSITKKAELD EITVDGLDAN QVWWQVKLVL DSIDGDLIQG IQELKDVVT PSHNLSDGST LNSSSGEESE LEEAESVFKE KQMLSADVSE IEEQSNDSLS ENDEEPSMDD EKTSAEAARE E FAEEKRIS SGQDERHSSP DPYGINDKFF DLEKFNRDTL AAEDSNEASE GSEDEDIDYF QDMPSDDEEE EAIYYEDFFD KP TKEPVKK HSDVKDPKED EELDEEEHDS AMDKVKLDLF ADEEDEPNAE GVGEASDKNL SSFEKQQIEI RKQIEQLENE AVA EKKWSL KGEVKAKDRP EDALLTEELE FDRTAKPVPV ITSEVTESLE DMIRRRIQDS NFDDLQRRTL LDITRKSQRP QFEL SDVKS SKSLAEIYED DYTRAEDESA LSEELQKAHS EISELYANLV YKLDVLSSVH FVPKPASTSL EIRVETPTIS MEDAQ PLYM SNASSLAPQE IYNVGKAEKD GEIRLKNGVA MSKEELTRED KNRLRRALKR KRSKANLPNV NKRSKRNDVV DTLSKA KNI TVINQKGEKK DVSGKTKKSR SGPDSTNIKL

UniProtKB: U3 small nucleolar RNA-associated protein MPP10

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Macromolecule #7: Ribosome biogenesis protein BMS1

MacromoleculeName: Ribosome biogenesis protein BMS1 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 135.792281 KDa
SequenceString: MEQSNKQHRK AKEKNTAKKK LHTQGHNAKA FAVAAPGKMA RTMQRSSDVN ERKLHVPMVD RTPEDDPPPF IVAVVGPPGT GKTTLIRSL VRRMTKSTLN DIQGPITVVS GKHRRLTFLE CPADDLNAMI DIAKIADLVL LLIDGNFGFE METMEFLNIA Q HHGMPRVL ...String:
MEQSNKQHRK AKEKNTAKKK LHTQGHNAKA FAVAAPGKMA RTMQRSSDVN ERKLHVPMVD RTPEDDPPPF IVAVVGPPGT GKTTLIRSL VRRMTKSTLN DIQGPITVVS GKHRRLTFLE CPADDLNAMI DIAKIADLVL LLIDGNFGFE METMEFLNIA Q HHGMPRVL GVATHLDLFK SQSTLRASKK RLKHRFWTEV YQGAKLFYLS GVINGRYPDR EILNLSRFIS VMKFRPLKWR NE HPYMLAD RFTDLTHPEL IETQGLQIDR KVAIYGYLHG TPLPSAPGTR VHIAGVGDFS VAQIEKLPDP CPTPFYQQKL DDF EREKMK EEAKANGEIT TASTTRRRKR LDDKDKLIYA PMSDVGGVLM DKDAVYIDIG KKNEEPSFVP GQERGEGEKL MTGL QSVEQ SIAEKFDGVG LQLFSNGTEL HEVADHEGMD VESGEESIED DEGKSKGRTS LRKPRIYGKP VQEEDADIDN LPSDE EPYT NDDDVQDSEP RMVEIDFNNT GEQGAEKLAL ETDSEFEESE DEFSWERTAA NKLKKTESKK RTWNIGKLIY MDNISP EEC IRRWRGEDDD SKDESDIEED VDDDFFRKKD GTVTKEGNKD HAVDLEKFVP YFDTFEKLAK KWKSVDAIKE RFLGAGI LG NDNKTKSDSN EGGEELYGDF EDLEDGNPSE QAEDNSDKES EDEDENEDTN GDDDNSFTNF DAEEKKDLTM EQEREMNA A KKEKLRAQFE IEEGENFKED DENNEYDTWY ELQKAKISKQ LEINNIEYQE MTPEQRQRIE GFKAGSYVRI VFEKVPMEF VKNFNPKFPI VMGGLLPTEI KFGIVKARLR RHRWHKKILK TNDPLVLSLG WRRFQTLPIY TTTDSRTRTR MLKYTPEHTY CNAAFYGPL CSPNTPFCGV QIVANSDTGN GFRIAATGIV EEIDVNIEIV KKLKLVGFPY KIFKNTAFIK DMFSSAMEVA R FEGAQIKT VSGIRGEIKR ALSKPEGHYR AAFEDKILMS DIVILRSWYP VRVKKFYNPV TSLLLKEKTE WKGLRLTGQI RA AMNLETP SNPDSAYHKI ERVERHFNGL KVPKAVQKEL PFKSQIHQMK PQKKKTYMAK RAVVLGGDEK KARSFIQKVL TIS KAKDSK RKEQKASQRK ERLKKLAKME EEKSQRDKEK KKEYFAQNGK RTTMGGDDES RPRKMRR

UniProtKB: Ribosome biogenesis protein BMS1

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Macromolecule #8: RNA 3'-terminal phosphate cyclase-like protein

MacromoleculeName: RNA 3'-terminal phosphate cyclase-like protein / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 40.220559 KDa
SequenceString: MSSSAPKYTT FQGSQNFRLR IVLATLSGKP IKIEKIRSGD LNPGLKDYEV SFLRLIESVT NGSVIEISYT GTTVIYRPGI IVGGASTHI CPSSKPVGYF VEPMLYLAPF SKKKFSILFK GITASHNDAG IEAIKWGLMP VMEKFGVREC ALHTLKRGSP P LGGGEVHL ...String:
MSSSAPKYTT FQGSQNFRLR IVLATLSGKP IKIEKIRSGD LNPGLKDYEV SFLRLIESVT NGSVIEISYT GTTVIYRPGI IVGGASTHI CPSSKPVGYF VEPMLYLAPF SKKKFSILFK GITASHNDAG IEAIKWGLMP VMEKFGVREC ALHTLKRGSP P LGGGEVHL VVDSLIAQPI TMHEIDRPII SSITGVAYST RVSPSLVNRM IDGAKKVLKN LQCEVNITAD VWRGENSGKS PG WGITLVA QSKQKGWSYF AEDIGDAGSI PEELGEKVAC QLLEEISKSA AVGRNQLPLA IVYMVIGKED IGRLRINKEQ IDE RFIILL RDIKKIFNTE VFLKPVDEAD NEDMIATIKG IGFTNTSKKI A

UniProtKB: rRNA processing protein RCL1

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Macromolecule #9: Ribosomal RNA small subunit methyltransferase NEP1

MacromoleculeName: Ribosomal RNA small subunit methyltransferase NEP1 / type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO
EC number: rRNA small subunit pseudouridine methyltransferase Nep1
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 27.936461 KDa
SequenceString: MVEDSRVRDA LKGGDQKALP ASLVPQAPPV LTSKDKITKR MIVVLAMASL ETHKISSNGP GGDKYVLLNC DDHQGLLKKM GRDISEARP DITHQCLLTL LDSPINKAGK LQVYIQTSRG ILIEVNPTVR IPRTFKRFSG LMVQLLHKLS IRSVNSEEKL L KVIKNPIT ...String:
MVEDSRVRDA LKGGDQKALP ASLVPQAPPV LTSKDKITKR MIVVLAMASL ETHKISSNGP GGDKYVLLNC DDHQGLLKKM GRDISEARP DITHQCLLTL LDSPINKAGK LQVYIQTSRG ILIEVNPTVR IPRTFKRFSG LMVQLLHKLS IRSVNSEEKL L KVIKNPIT DHLPTKCRKV TLSFDAPVIR VQDYIEKLDD DESICVFVGA MARGKDNFAD EYVDEKVGLS NYPLSASVAC SK FCHGAED AWNIL

UniProtKB: Ribosomal RNA small subunit methyltransferase NEP1

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Macromolecule #10: Essential nuclear protein 1

MacromoleculeName: Essential nuclear protein 1 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 55.207422 KDa
SequenceString: MARASSTKAR KQRHDPLLKD LDAAQGTLKK INKKKLAQND AANHDAANEE DGYIDSKASR KILQLAKEQQ DEIEGEELAE SERNKQFEA RFTTMSYDDE DEDEDEDEEA FGEDISDFEP EGDYKEEEEI VEIDEEDAAM FEQYFKKSDD FNSLSGSYNL A DKIMASIR ...String:
MARASSTKAR KQRHDPLLKD LDAAQGTLKK INKKKLAQND AANHDAANEE DGYIDSKASR KILQLAKEQQ DEIEGEELAE SERNKQFEA RFTTMSYDDE DEDEDEDEEA FGEDISDFEP EGDYKEEEEI VEIDEEDAAM FEQYFKKSDD FNSLSGSYNL A DKIMASIR EKESQVEDMQ DDEPLANEQN TSRGNISSGL KSGEGVALPE KVIKAYTTVG SILKTWTHGK LPKLFKVIPS LR NWQDVIY VTNPEEWSPH VVYEATKLFV SNLTAKESQK FINLILLERF RDNIETSEDH SLNYHIYRAV KKSLYKPSAF FKG FLFPLV ETGCNVREAT IAGSVLAKVS VPALHSSAAL SYLLRLPFSP PTTVFIKILL DKKYALPYQT VDDCVYYFMR FRIL DDGSN GEDATRVLPV IWHKAFLTFA QRYKNDITQD QRDFLLETVR QRGHKDIGPE IRRELLAGAS REFVDPQEAN DDLMI DVN

UniProtKB: Essential nuclear protein 1

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Macromolecule #11: Dimethyladenosine transferase

MacromoleculeName: Dimethyladenosine transferase / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
EC number: 18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 36.003836 KDa
SequenceString: MGKAAKKKYS GATSSKQVSA EKHLSSVFKF NTDLGQHILK NPLVAQGIVD KAQIRPSDVV LEVGPGTGNL TVRILEQAKN VVAVEMDPR MAAELTKRVR GTPVEKKLEI MLGDFMKTEL PYFDICISNT PYQISSPLVF KLINQPRPPR VSILMFQREF A LRLLARPG ...String:
MGKAAKKKYS GATSSKQVSA EKHLSSVFKF NTDLGQHILK NPLVAQGIVD KAQIRPSDVV LEVGPGTGNL TVRILEQAKN VVAVEMDPR MAAELTKRVR GTPVEKKLEI MLGDFMKTEL PYFDICISNT PYQISSPLVF KLINQPRPPR VSILMFQREF A LRLLARPG DSLYCRLSAN VQMWANVTHI MKVGKNNFRP PPQVESSVVR LEIKNPRPQV DYNEWDGLLR IVFVRKNRTI SA GFKSTTV MDILEKNYKT FLAMNNEMVD DTKGSMHDVV KEKIDTVLKE TDLGDKRAGK CDQNDFLRLL YAFHQVGIHF S

UniProtKB: Dimethyladenosine transferase

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Macromolecule #12: Pre-rRNA-processing protein PNO1

MacromoleculeName: Pre-rRNA-processing protein PNO1 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 30.380623 KDa
SequenceString: MVAPTALKKA TVTPVSGQDG GSSRIIGINN TESIDEDDDD DVLLDDSDNN TAKEEVEGEE GSRKTHESKT VVVDDQGKPR FTSASKTQG NKIKFESRKI MVPPHRMTPL RNSWTKIYPP LVEHLKLQVR MNLKTKSVEL RTNPKFTTDP GALQKGADFI K AFTLGFDL ...String:
MVAPTALKKA TVTPVSGQDG GSSRIIGINN TESIDEDDDD DVLLDDSDNN TAKEEVEGEE GSRKTHESKT VVVDDQGKPR FTSASKTQG NKIKFESRKI MVPPHRMTPL RNSWTKIYPP LVEHLKLQVR MNLKTKSVEL RTNPKFTTDP GALQKGADFI K AFTLGFDL DDSIALLRLD DLYIETFEVK DVKTLTGDHL SRAIGRIAGK DGKTKFAIEN ATRTRIVLAD SKIHILGGFT HI RMARESV VSLILGSPPG KVYGNLRTVA SRLKERY

UniProtKB: Pre-rRNA-processing protein PNO1

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Macromolecule #13: Rps5p

MacromoleculeName: Rps5p / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 25.0726 KDa
SequenceString: MSDTEAPVEV QEDFEVVEEF TPVVLATPIP EEVQQAQTEI KLFNKWSFEE VEVKDASLVD YVQVRQPIFV AHTAGRYANK RFRKAQCPI IERLTNSLMM NGRNNGKKLK AVRIIKHTLD IINVLTDQNP IQVVVDAITN TGPREDTTRV GGGGAARRQA V DVSPLRRV ...String:
MSDTEAPVEV QEDFEVVEEF TPVVLATPIP EEVQQAQTEI KLFNKWSFEE VEVKDASLVD YVQVRQPIFV AHTAGRYANK RFRKAQCPI IERLTNSLMM NGRNNGKKLK AVRIIKHTLD IINVLTDQNP IQVVVDAITN TGPREDTTRV GGGGAARRQA V DVSPLRRV NQAIALLTIG AREAAFRNIK TIAETLAEEL INAAKGSSTS YAIKKKDELE RVAKSNR

UniProtKB: Small ribosomal subunit protein uS7

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Macromolecule #14: 40S ribosomal protein S16-A

MacromoleculeName: 40S ribosomal protein S16-A / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 15.87749 KDa
SequenceString:
MSAVPSVQTF GKKKSATAVA HVKAGKGLIK VNGSPITLVE PEILRFKVYE PLLLVGLDKF SNIDIRVRVT GGGHVSQVYA IRQAIAKGL VAYHQKYVDE QSKNELKKAF TSYDRTLLIA DSRRPEPKKF GGKGARSRFQ KSYR

UniProtKB: Small ribosomal subunit protein uS9A

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Macromolecule #15: 40S ribosomal protein S18-A,40S ribosomal protein S18-A,Rps18

MacromoleculeName: 40S ribosomal protein S18-A,40S ribosomal protein S18-A,Rps18
type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 16.303852 KDa
SequenceString: MSLVVQEQGS FQHILRLLNT NVDGNIKIVY ALTTIKGVGR RYSNLVCKKA DVDLHKRAGE LTQEELERIV QIMQNPTHYK IPAWFLNRQ NDITDGKDYH TLANNVESKL RDDLERLKKI RAH(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) ...String:
MSLVVQEQGS FQHILRLLNT NVDGNIKIVY ALTTIKGVGR RYSNLVCKKA DVDLHKRAGE LTQEELERIV QIMQNPTHYK IPAWFLNRQ NDITDGKDYH TLANNVESKL RDDLERLKKI RAH(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

UniProtKB: Small ribosomal subunit protein uS13A

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Macromolecule #16: 40S ribosomal protein S19-A

MacromoleculeName: 40S ribosomal protein S19-A / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 15.942125 KDa
SequenceString:
MPGVSVRDVA AQDFINAYAS FLQRQGKLEV PGYVDIVKTS SGNEMPPQDA EGWFYKRAAS VARHIYMRKQ VGVGKLNKLY GGAKSRGVR PYKHIDASGS INRKVLQALE KIGIVEISPK GGRRISENGQ RDLDRIAAQT LEEDE

UniProtKB: Small ribosomal subunit protein eS19A

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Macromolecule #17: 40S ribosomal protein S28-A

MacromoleculeName: 40S ribosomal protein S28-A / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 7.605847 KDa
SequenceString:
MDNKTPVTLA KVIKVLGRTG SRGGVTQVRV EFLEDTSRTI VRNVKGPVRE NDILVLMESE REARRLR

UniProtKB: Small ribosomal subunit protein eS28A

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Macromolecule #19: 40S ribosomal protein S1-A

MacromoleculeName: 40S ribosomal protein S1-A / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 28.798467 KDa
SequenceString: MAVGKNKRLS KGKKGQKKRV VDPFTRKEWF DIKAPSTFEN RNVGKTLVNK STGLKSASDA LKGRVVEVCL ADLQGSEDHS FRKIKLRVD EVQGKNLLTN FHGMDFTTDK LRSMVRKWQT LIEANVTVKT SDDYVLRIFA IAFTRKQANQ VKRHSYAQSS H IRAIRKVI ...String:
MAVGKNKRLS KGKKGQKKRV VDPFTRKEWF DIKAPSTFEN RNVGKTLVNK STGLKSASDA LKGRVVEVCL ADLQGSEDHS FRKIKLRVD EVQGKNLLTN FHGMDFTTDK LRSMVRKWQT LIEANVTVKT SDDYVLRIFA IAFTRKQANQ VKRHSYAQSS H IRAIRKVI SEILTKEVQG STLAQLTSKL IPEVINKEIE NATKDIFPLQ NIHVRKVKLL KQPKFDVGAL MALHGEGSGE EK GKKVTGF KDEVLETV

UniProtKB: Small ribosomal subunit protein eS1A

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Macromolecule #20: 40S ribosomal protein S4-A

MacromoleculeName: 40S ribosomal protein S4-A / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 29.46933 KDa
SequenceString: MARGPKKHLK RLAAPHHWLL DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN RLKYALNGRE VKAILMQRHV KVDGKVRTDT TYPAGFMDV ITLDATNENF RLVYDVKGRF AVHRITDEEA SYKLGKVKKV QLGKKGVPYV VTHDGRTIRY PDPNIKVNDT V KIDLASGK ...String:
MARGPKKHLK RLAAPHHWLL DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN RLKYALNGRE VKAILMQRHV KVDGKVRTDT TYPAGFMDV ITLDATNENF RLVYDVKGRF AVHRITDEEA SYKLGKVKKV QLGKKGVPYV VTHDGRTIRY PDPNIKVNDT V KIDLASGK ITDFIKFDAG KLVYVTGGRN LGRIGTIVHK ERHDGGFDLV HIKDSLDNTF VTRLNNVFVI GEQGKPYISL PK GKGIKLS IAEERDRRRA QQGL

UniProtKB: Small ribosomal subunit protein eS4A

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Macromolecule #21: 40S ribosomal protein S6-A

MacromoleculeName: 40S ribosomal protein S6-A / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 27.054486 KDa
SequenceString: MKLNISYPVN GSQKTFEIDD EHRIRVFFDK RIGQEVDGEA VGDEFKGYVF KISGGNDKQG FPMKQGVLLP TRIKLLLTKN VSCYRPRRD GERKRKSVRG AIVGPDLAVL ALVIVKKGEQ ELEGLTDTTV PKRLGPKRAN NIRKFFGLSK EDDVRDFVIR R EVTKGEKT ...String:
MKLNISYPVN GSQKTFEIDD EHRIRVFFDK RIGQEVDGEA VGDEFKGYVF KISGGNDKQG FPMKQGVLLP TRIKLLLTKN VSCYRPRRD GERKRKSVRG AIVGPDLAVL ALVIVKKGEQ ELEGLTDTTV PKRLGPKRAN NIRKFFGLSK EDDVRDFVIR R EVTKGEKT YTKAPKIQRL VTPQRLQRKR HQRALKVRNA QAQREAAAEY AQLLAKRLSE RKAEKAEIRK RRASSLKA

UniProtKB: Small ribosomal subunit protein eS6A

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Macromolecule #22: 40S ribosomal protein S7-A

MacromoleculeName: 40S ribosomal protein S7-A / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 21.658209 KDa
SequenceString:
MSAPQAKILS QAPTELELQV AQAFVELENS SPELKAELRP LQFKSIREID VAGGKKALAI FVPVPSLAGF HKVQTKLTRE LEKKFQDRH VIFLAERRIL PKPSRTSRQV QKRPRSRTLT AVHDKILEDL VFPTEIVGKR VRYLVGGNKI QKVLLDSKDV Q QIDYKLES FQAVYNKLTG KQIVFEIPSE TH

UniProtKB: Small ribosomal subunit protein eS7A

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Macromolecule #23: 40S ribosomal protein S8-A

MacromoleculeName: 40S ribosomal protein S8-A / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 22.537803 KDa
SequenceString: MGISRDSRHK RSATGAKRAQ FRKKRKFELG RQPANTKIGA KRIHSVRTRG GNKKYRALRI ETGNFSWASE GISKKTRIAG VVYHPSNNE LVRTNTLTKA AIVQIDATPF RQWFEAHYGQ TLGKKKNVKE EETVAKSKNA ERKWAARAAS AKIESSVESQ F SAGRLYAC ...String:
MGISRDSRHK RSATGAKRAQ FRKKRKFELG RQPANTKIGA KRIHSVRTRG GNKKYRALRI ETGNFSWASE GISKKTRIAG VVYHPSNNE LVRTNTLTKA AIVQIDATPF RQWFEAHYGQ TLGKKKNVKE EETVAKSKNA ERKWAARAAS AKIESSVESQ F SAGRLYAC ISSRPGQSGR CDGYILEGEE LAFYLRRLTA KK

UniProtKB: Small ribosomal subunit protein eS8A

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Macromolecule #24: 40S ribosomal protein S9-A

MacromoleculeName: 40S ribosomal protein S9-A / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 22.487893 KDa
SequenceString: MPRAPRTYSK TYSTPKRPYE SSRLDAELKL AGEFGLKNKK EIYRISFQLS KIRRAARDLL TRDEKDPKRL FEGNALIRRL VRVGVLSED KKKLDYVLAL KVEDFLERRL QTQVYKLGLA KSVHHARVLI TQRHIAVGKQ IVNIPSFMVR LDSEKHIDFA P TSPFGGAR ...String:
MPRAPRTYSK TYSTPKRPYE SSRLDAELKL AGEFGLKNKK EIYRISFQLS KIRRAARDLL TRDEKDPKRL FEGNALIRRL VRVGVLSED KKKLDYVLAL KVEDFLERRL QTQVYKLGLA KSVHHARVLI TQRHIAVGKQ IVNIPSFMVR LDSEKHIDFA P TSPFGGAR PGRVARRNAA RKAEASGEAA DEADEADEE

UniProtKB: Small ribosomal subunit protein uS4A

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Macromolecule #25: 40S ribosomal protein S11-A

MacromoleculeName: 40S ribosomal protein S11-A / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 17.785934 KDa
SequenceString:
MSTELTVQSE RAFQKQPHIF NNPKVKTSKR TKRWYKNAGL GFKTPKTAIE GSYIDKKCPF TGLVSIRGKI LTGTVVSTKM HRTIVIRRA YLHYIPKYNR YEKRHKNVPV HVSPAFRVQV GDIVTVGQCR PISKTVRFNV VKVSAAAGKA NKQFAKF

UniProtKB: Small ribosomal subunit protein uS17A

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Macromolecule #26: 40S ribosomal protein S13

MacromoleculeName: 40S ribosomal protein S13 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 17.059945 KDa
SequenceString:
MGRMHSAGKG ISSSAIPYSR NAPAWFKLSS ESVIEQIVKY ARKGLTPSQI GVLLRDAHGV TQARVITGNK IMRILKSNGL APEIPEDLY YLIKKAVSVR KHLERNRKDK DAKFRLILIE SRIHRLARYY RTVAVLPPNW KYESATASAL VN

UniProtKB: Small ribosomal subunit protein uS15

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Macromolecule #27: 40S ribosomal protein S14-A

MacromoleculeName: 40S ribosomal protein S14-A / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 14.562655 KDa
SequenceString:
MSNVVQARDN SQVFGVARIY ASFNDTFVHV TDLSGKETIA RVTGGMKVKA DRDESSPYAA MLAAQDVAAK CKEVGITAVH VKIRATGGT RTKTPGPGGQ AALRALARSG LRIGRIEDVT PVPSDSTRKK GGRRGRRL

UniProtKB: Small ribosomal subunit protein uS11A

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Macromolecule #28: 40S ribosomal protein S25-A

MacromoleculeName: 40S ribosomal protein S25-A / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 12.067272 KDa
SequenceString:
MPPKQQLSKA AKAAAALAGG KKSKKKWSKK SMKDRAQHAV ILDQEKYDRI LKEVPTYRYV SVSVLVDRLK IGGSLARIAL RHLEKEGII KPISKHSKQA IYTRATASE

UniProtKB: Small ribosomal subunit protein eS25A

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Macromolecule #29: 40S ribosomal protein S22-A

MacromoleculeName: 40S ribosomal protein S22-A / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 14.650062 KDa
SequenceString:
MTRSSVLADA LNAINNAEKT GKRQVLIRPS SKVIIKFLQV MQKHGYIGEF EYIDDHRSGK IVVQLNGRLN KCGVISPRFN VKIGDIEKW TANLLPARQF GYVILTTSAG IMDHEEARRK HVSGKILGFV Y

UniProtKB: Small ribosomal subunit protein uS8A

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Macromolecule #30: 40S ribosomal protein S23-A

MacromoleculeName: 40S ribosomal protein S23-A / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 16.073896 KDa
SequenceString:
MGKGKPRGLN SARKLRVHRR NNRWAENNYK KRLLGTAFKS SPFGGSSHAK GIVLEKLGIE SKQPNSAIRK CVRVQLIKNG KKVTAFVPN DGCLNFVDEN DEVLLAGFGR KGKAKGDIPG VRFKVVKVSG VSLLALWKEK KEKPRS

UniProtKB: Small ribosomal subunit protein uS12A

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Macromolecule #31: 40S ribosomal protein S24-A

MacromoleculeName: 40S ribosomal protein S24-A / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 15.362848 KDa
SequenceString:
MSDAVTIRTR KVISNPLLAR KQFVVDVLHP NRANVSKDEL REKLAEVYKA EKDAVSVFGF RTQFGGGKSV GFGLVYNSVA EAKKFEPTY RLVRYGLAEK VEKASRQQRK QKKNRDKKIF GTGKRLAKKV ARRNAD

UniProtKB: Small ribosomal subunit protein eS24A

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Macromolecule #32: 40S ribosomal protein S27-A

MacromoleculeName: 40S ribosomal protein S27-A / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 8.893391 KDa
SequenceString:
MVLVQDLLHP TAASEARKHK LKTLVQGPRS YFLDVKCPGC LNITTVFSHA QTAVTCESCS TILCTPTGGK AKLSEGTSFR RK

UniProtKB: Small ribosomal subunit protein eS27A

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Macromolecule #33: U3 small nucleolar RNA-associated protein 14

MacromoleculeName: U3 small nucleolar RNA-associated protein 14 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 103.18975 KDa
SequenceString: MAKKKSKSRS KSSRRVLDAL QLAEREINGE FDNSSDNDKR HDARRNGTVV NLLKRSKGDT NSDEDDIDSE SFEDEELNSD EALGSDDDY DILNSKFSQT IRDKKENANY QEEEDEGGYT SIDEEDLMPL SQVWDMDEKT AQSNGNDDED ASPQLKLQDT D ISSESSSS ...String:
MAKKKSKSRS KSSRRVLDAL QLAEREINGE FDNSSDNDKR HDARRNGTVV NLLKRSKGDT NSDEDDIDSE SFEDEELNSD EALGSDDDY DILNSKFSQT IRDKKENANY QEEEDEGGYT SIDEEDLMPL SQVWDMDEKT AQSNGNDDED ASPQLKLQDT D ISSESSSS EESESESEDD EEEEDPFDEI SEDEEDIELN TITSKLIDET KSKAPKRLDT YGSGEANEYV LPSANAASGA SG KLSLTDM MNVIDDRQVI ENANLLKGKS STYEVPLPQR IQQRHDRKAA YEISRQEVSK WNDIVQQNRR ADHLIFPLNK PTE HNHASA FTRTQDVPQT ELQEKVDQVL QESNLANPEK DSKFEELSTA KMTPEEMRKR TTEMRLMREL MFREERKARR LKKI KSKTY RKIKKKELMK NRELAAVSSD EDNEDHDIAR AKERMTLKHK TNSKWAKDMI KHGMTNDAET REEMEEMLRQ GERLK AKML DRNSDDEEDG RVQTLSDVEN EEKENIDSEA LKSKLGKTGV MNMAFMKNGE AREREANKET LRQLRAVENG DDIKLF ESD EEETNGENIQ INKGRRIYTP GSLESNKDMN ELNDHTRKEN KVDESRSLEN RLRAKNSGQS KNARTNAEGA IIVEEES DG EPLQDGQNNQ QDEEAKDVNP WLANESDEEH TVKKQSSKVN VIDKDSSKNV KAMNKMEKAE LKQKKKKKGK SNDDEDLL L TADDSTRLKI VDPYGGSDDE QGDNVFMFKQ QDVIAEAFAG DDVVAEFQEE KKRVIDDEDD KEVDTTLPGW GEWAGAGSK PKNKKRKFIK KVKGVVNKDK RRDKNLQNVI INEKVNKKNL KYQSSAVPFP FENREQYERS LRMPIGQEWT SRASHQELIK PRIMTKPGQ VIDPLKAPFK

UniProtKB: U3 small nucleolar RNA-associated protein 14

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Macromolecule #34: Probable ATP-dependent RNA helicase DHR1

MacromoleculeName: Probable ATP-dependent RNA helicase DHR1 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA helicase
Source (natural)Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (yeast)
Strain: ATCC 204508 / S288c
Molecular weightTheoretical: 145.171406 KDa
SequenceString: MGTYRKRFNE KARSGHMAKL KELKRIRNKQ FTRQDENDER VENPDSAPAE SSTTEPNANA EILEPLTEEE KKMKKRKLQE LFTPKESKV SRLKKKRLDK FIEHQLKREE RKTIIGKLQD YKIDTSLLTS SKRLGEGRQT KKEEFKEALS LERQGRGNEQ T NEILYEEY ...String:
MGTYRKRFNE KARSGHMAKL KELKRIRNKQ FTRQDENDER VENPDSAPAE SSTTEPNANA EILEPLTEEE KKMKKRKLQE LFTPKESKV SRLKKKRLDK FIEHQLKREE RKTIIGKLQD YKIDTSLLTS SKRLGEGRQT KKEEFKEALS LERQGRGNEQ T NEILYEEY EPKVWDEYGE GGSSEDDDGE DDFEASFGSM PKPTDNEEKK SSGFIDHRPA KFGGSGLSFG FSNIKVINKE SK TPKKKYN WRQRVEMEEL KKHGKEDEMD FDTTSEDDDE EEDQEEEDKM HPSENPLEEV ESADSETGSE KFDQNDVANE FKD WANQEI KKLEGRDQEL VTPTLNIDYK PIIRKEDLDD GLQEAYVPIN ENSTRKAFYV EVSRSDEIQK ARIQLPVFGE EHKI MEAIH HNDVVIICGE TGSGKTTQVP QFLYEAGFGA EDSPDYPGMV GITQPRRVAA VSMAERVANE LGDHGHKVGY QIRFD STAK EDTKVKFMTD GVLLREMMHD FKLTKYSSII IDEAHERNIN TDILIGMLSR CVRLRAKLHK ENPIEHKKLK LIIMSA TLR VSDFSENKTL FPIAPPVLQV DARQFPVSIH FNRRTAFNYT DEAFRKTCKI HQKLPPGAIL VFLTGQQEIT HMVKRLR KE FPFKKNSKYN KDLETPVSKM GINSKTTDLE AEDIDFSVQV IDQDKFKSAI RYEEDEGNSG NGEDEEDEEE EGFEEVLT E GQTANDPLYV LPLYSLLPTK EQMRVFQKPP QGSRLCIVAT NVAETSLTIP GVRYVVDSGR SKERKYNESN GVQSFEVGW VSKASANQRS GRAGRTGPGH CYRLYSSAVF EHDFEQFSKP EILRMPVESI VLQMKSMAIH NIINFPFPTP PDRVALSKAI QLLQYLGAL DNKEMITEDG KKMSLFPLSP RFSKMLLVSD EKACLPYIVA IVSALSVGDP FINEFELGIN EISRKPNPDE N LDDKIREH DESTPGMDPE LKKELRSKFY KSRSQFSKLD KFSDVFRLLS VVSAMDYVPK EQKEIFMKKN FLRGKLMEEI VK LRKQLMY IIKSNTSKEN IAVVIRNEDL KSDIPSVIQI KLLKQMICAG FVDHVAVRAD VLFPDDAKIT NRTSIINIPY IPV LATRTP NIEDCFVYIH PTSILNNLGE MPPKYMLYYS LHLGGNNKTR MNTLCDIAST PLANIARKGL LLTYSKPLTG QGLK TVNLS PTERYCYVVP RFGSTVDNDL KIGWDLNPIA VHQKKQKGQW TVIKFITRKG FQTITGEEKE KK

UniProtKB: Probable ATP-dependent RNA helicase DHR1

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Macromolecule #18: 18S rRNA

MacromoleculeName: 18S rRNA / type: rna / ID: 18 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 566.062188 KDa
SequenceString: UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA UUUGAUAGUU CCUUUACUAC AUGGUAUAAC UGUGGUAAUU C UAGAGCUA ...String:
UAUCUGGUUG AUCCUGCCAG UAGUCAUAUG CUUGUCUCAA AGAUUAAGCC AUGCAUGUCU AAGUAUAAGC AAUUUAUACA GUGAAACUG CGAAUGGCUC AUUAAAUCAG UUAUCGUUUA UUUGAUAGUU CCUUUACUAC AUGGUAUAAC UGUGGUAAUU C UAGAGCUA AUACAUGCUU AAAAUCUCGA CCCUUUGGAA GAGAUGUAUU UAUUAGAUAA AAAAUCAAUG UCUUCGGACU CU UUGAUGA UUCAUAAUAA CUUUUCGAAU CGCAUGGCCU UGUGCUGGCG AUGGUUCAUU CAAAUUUCUG CCCUAUCAAC UUU CGAUGG UAGGAUAGUG GCCUACCAUG GUUUCAACGG GUAACGGGGA AUAAGGGUUC GAUUCCGGAG AGGGAGCCUG AGAA ACGGC UACCACAUCC AAGGAAGGCA GCAGGCGCGC AAAUUACCCA AUCCUAAUUC AGGGAGGUAG UGACAAUAAA UAACG AUAC AGGGCCCAUU CGGGUCUUGU AAUUGGAAUG AGUACAAUGU AAAUACCUUA ACGAGGAACA AUUGGAGGGC AAGUCU GGU GCCAGCAGCC GCGGUAAUUC CAGCUCCAAU AGCGUAUAUU AAAGUUGUUG CAGUUAAAAA GCUCGUAGUU GAACUUU GG GCCCGGUUGG CCGGUCCGAU UUUUUCGUGU ACUGGAUUUC CAACGGGGCC UUUCCUUCUG GCUAACCUUG AGUCCUUG U GGCUCUUGGC GAACCAGGAC UUUUACUUUG AAAAAAUUAG AGUGUUCAAA GCAGGCGUAU UGCUCGAAUA UAUUAGCAU GGAAUAAUAG AAUAGGACGU UUGGUUCUAU UUUGUUGGUU UCUAGGACCA UCGUAAUGAU UAAUAGGGAC GGUCGGGGGC AUCAGUAUU CAAUUGUCAG AGGUGAAAUU CUUGGAUUUA UUGAAGACUA ACUACUGCGA AAGCAUUUGC CAAGGACGUU U UCAUUAAU CAAGAACGAA AGUUAGGGGA UCGAAGAUGA UCAGAUACCG UCGUAGUCUU AACCAUAAAC UAUGCCGACU AG GGAUCGG GUGGUGUUUU UUUAAUGACC CACUCGGCAC CUUACGAGAA AUCAAAGUCU UUGGGUUCUG GGGGGAGUAU GGU CGCAAG GCUAAAACUU AAAGGAAUUG ACGGAAGGGC ACCACCAGGA GUGGAGCCUG CGGCUUAAUU UGACUCAACA CGGG GAAAC UCACCAGGUC CAGACACAAU AAGGAUUGAC AGAUUGAGAG CUCUUUCUUG AUUUUGUGGG UGGUGGUGCA UGGCC GUUC UUAGUUGGUG GAGUGAUUUG UCUGCUUAAU UGCGAUAACG AACGAGACCU UAACCUACUA AAUAGUGGUG CUAGCA UUU GCUGGUUAUC CACUUCUUAG AGGGACUAUC GGUUUCAAGC CGAUGGAAGU UUGAGGCAAU AACAGGUCUG UGAUGCC CU UAGACGUUCU GGGCCGCACG CGCGCUACAC UGACGGAGCC AGCGAGUCUA ACCUUGGCCG AGAGGUCUUG GUAAUCUU G UGAAACUCCG UCGUGCUGGG GAUAGAGCAU UGUAAUUAUU GCUCUUCAAC GAGGAAUUCC UAGUAAGCGC AAGUCAUCA GCUUGCGUUG AUUACGUCCC UGCCCUUUGU ACACACCGCC CGUCGCUAGU ACCGAUUUCC AUCUCAGAGC GGAGAAUUUG GACAAACUU GGUCAUUUAG AGGAACUAAA AGUCGUAACA AGGUUUCCGU AGGUGAACCU GCGGAAGGAU CAUUAAAGA

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Macromolecule #35: U3 snoRNA

MacromoleculeName: U3 snoRNA / type: rna / ID: 35 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 7.459431 KDa
SequenceString:
(M7G)GUCGACGUA CUUCAUAGGA UCA

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Macromolecule #36: Poly-U RNA

MacromoleculeName: Poly-U RNA / type: rna / ID: 36 / Number of copies: 1
Source (natural)Organism: Saccharomyces cerevisiae S288C (yeast)
Molecular weightTheoretical: 2.710535 KDa
SequenceString:
UUUUUUUUU

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Macromolecule #37: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 37 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #38: GUANOSINE-5'-TRIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 38 / Number of copies: 1 / Formula: GTP
Molecular weightTheoretical: 523.18 Da
Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

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Macromolecule #39: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 39 / Number of copies: 39 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
GridModel: Quantifoil / Material: COPPER / Support film - Material: CARBON
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 44.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 102097
Initial angle assignmentType: OTHER / Details: Relion
Final angle assignmentType: OTHER / Details: Relion
FSC plot (resolution estimation)

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